The basic construction of VersaPulse PowerSuite 100W holmium laser system is introduced, then focuses on the discussion of its application security control, application furthermore, and analyses its preoperative, intraoperative and postoperative manipulation, and finally elaborates on the corresponding calibration process.

ObjectiveTo evaluate the clinical efficacy and safty of combined treatment telmisartan plus simvastatin on early diabetic nephropathy. Methods46 patients with early diabetic nephropathy were randomly divided into two groups. Control group ( n ＝ 23 ) received regular insulin therapy, captopril l0mg tid. Study group received telmisartan 80mg qd plus simvastatin 20mg qd on the basis of control group therapy. All patients received 16 weeks treatment. The clinical parameters were measured before and after therapy. ResultsAfter 16 weeks treatment,the urine MAU in both study group and control group were significantly decreased( all P ＜0. 01 ) ,however,the urine MAU in telmisartan plus simvastatin combination group decreased significantly than control group( P ＜ 0. 01 ). Improvement of lipid level in the study group better than the control group( P ＜0. 01 ). Kidney function,fasting blood glucose levels and HbAlc had no significant changes before and after treatment. ConclusionTelmisartan plus simvastatin combined treatment was better than the traditional therapy,which can delay the development of early diabetic nephropathy.

Objective To investigate the effect of infrasound on the growth of bone marrow mescenchymal stem cells (BMSCs) and seek the most reasonable duration for using infrasound. Methods The primary BMSCs were obtained from Sprague-Dawley rats through whole bone marrow adherent cultivation.The cells of passage 3 were divided into trial groups treated with infrasound for 10 min,30 min or 60 min,and control groups which were not treated with infrasound but exposed to air for the same durations.The vitality of cell proliferation was measured using the CCK8 method.Apoptosis and the cell cycle were analysed with flow cytometry (FACS). Results After cultivation for 72 h,the optical density (OD) values for BMSCs treated with infrasound for 10 min,30 min and 60 min were 1.480 ± 0.030,1.348 ± 0.030,1.493 ± 0.030 respectively and after 96 h they were 1.774 ± 0.030,1.731 ± 0.030 and 1.833 ± 0.030.All of which were significantly greater than in the control groups ( 1.479 ± 0.030,1.267 ±0.030 and 1.227 ± 0.030 after 72 h and 1.567 ± 0.030,1.563 ± 0.030 and 1.632 ± 0.030 after 96 h).With the extension of the treatment duration,the OD values of the BMSCs increased or increased after decreasing,and the OD value for BMSCs treated with infrasound for 60 min was the highest.The FACS results indicated no effect of infrasound on apoptosis of BMSCs when the treatment duration was 30 min,but that cell apoptosis could be inhibited when the treatment duration was 10 min or 60 min.The early phase apoptosis rates were 1.07％ ± 0.12％ and 0.97％ ±0.21％ in the trial groups treated for 10 min and 60 min respectively,and 1.43％ ± 0.06％ and 3.33％ ± 0.15％ in the respective control groups,a highly significant difference.The results of cell cycle analysis showed infrasound could disturb the cell division of BMSCs significantly when the treatment duration was less than 30 min,but there was no significant effect when the treatment duration was 60 min. Conclusions Infrasound can promote cell proliferation and disturb cell division,but it did not cause apoptosis of BMSCs.When the treatment duration was 60 min,cell proliferation improved steadily,the apoptosis rate decreased,and the cell cycle did not change.So 60 min is the most reasonable treatment duration for infrasound.

Objective To investigate the expressions of estrogen receptor (ER) and progesterone receptor (PR) in different tumor tissues of invasive ductal carcinoma(IDC) of the breast. Methods A total of 32 cases of IDC samples were collected. ER and PR expressions in 4 different sites of every sample were measured by immunohistochemical(IHC) assays. Results Overall, a high concordance in both estrogen receptor and progesterone receptor expressions are observed between different tumor tissue sites. The best Kappa values were 0.789 and 0.810 for ER and PR expression respectively. The worst Kappa values were 0. 563 and 0.672,respectively. Conclusion ER and PR expression is not significantly different in different tumor tissues of the same IDC sample. Repeated testing in different tumor tissue sites has little contributions in clinical settings.

Objective To investigate the value of SPECT imaging in the evaluation of rCBF improvement after bypass surgery in patients with moyamoya disease (MMD).Methods Retrospective analysis was conducted in 13 patients with MMD (5 children aged 3-8 years and 8 adults aged 45-55 years).The SPECT cerebral perfusion imaging data before and after operation were analyzed.Age matched subjects who underwent cerebral perfusion imaging for accidental headache (10 children,10 adults) were taken as control group.Two-sample t test or paired t test was used to compare rCBF of different groups.The brain DSA and MRI results during follow-up were taken as the reference of perfusion improvement.Results Based on visual judgment of SPECT,11/13 in MMD group showed significant improvement in the blood perfusion reduction area after surgery.The coincidence rates of SPECT-DSA and SPECT-MRI was 8/11 and 9/11,respectively.The rCBF of the control group and MMD group before surgery was (51.19±1.63) ml · 100 g-1 · min-1 and (38.83±2.22) ml · 100 g-1 · min-1 respectively (t=-18.465,P＜0.05).The rCBF of MMD group before and after surgery was significantly different ((38.83±2.22) ml · 100 g-1 · min-1 vs (45.03±2.63) ml · 100 g-1 · min-1,t=-11.707,P＜0.05).The rCBF of MMD group after surgery and the control group was significantly different (t =-8.313,P＜0.05).There was no significant difference of rCBF between the children and adults in the control group ((51.81±1.82) ml · 100 g-1 · min-1 vs (50.55±1.18) ml · 100 g-1 · min-1 ;t=0.081,P＞0.05).Conclusions Brain perfusion SPECT may effectively reflect the regional distribution and changes of blood flow in the ischemic tissue before and after MMD surgery.Thus it may be helpful in evaluation of the effect of surgical treatment.

Objective To evaluate the clinical value of serum high density lipoprotein cholesterol (HDL-C)in the evaluation of synthetic function of the patients with liver diseases.Methods Abbott Aeroset automatic biochemical analyzer was used to detect serum HDL-C level in control group (30 cases) and liver disease group(87 cases).T test was used to analyze the differences of serum HDL-C level in liver disease group and control group,different liver disease groups and control group,and different liver disease groups.Results HDL-C level was (1.28 ±0.20)mmol/L in the control group,(0.77 ± 0.15)mmol/L in the liver disease group,and (0.81 ± 0.13)mmol/L in the hepatitis group,(0.68 ± 0.14)mmol/L in the liver cirrhosis group,(0.54 ± 0.05)mmol/L in the liver cancer group.The HDL-C level in liver disease group and hepatitis group,liver cirrhosis group,liver cancer group was significantly lower than that in the control group(t =15.5569,14.2463,45.4393,25.6344,all P ＜ 0.01).The HDL-C level in liver cirrhosis group and liver cancer group was significantly lower than that in hepatitis group (t =3.6583,16.9057,all P ＜ 0.01).The HDL-C level in liver cancer group was significantly lower than that in liver cirrhosis group(t =4.4103,P ＜ 0.01).Conclusion Serum HDL-C level can be used to evaluate synthetic function of the liver,the lower of serum HDL-C level,the more serious liver synthesis function is impaired.

Purpose:To evaluate the feasibility of balloon catheter dilatation and internal stent placement in the treatment of Takayasu's arterial stenosis.Materials and Methods:Three patients with stenosis of common carotid arteries caused by Takayasu' s disease were treated with PTA by balloon catheter and intravaseular stent placement,the lenghs of stenosis were all beyond 8cm.Balloon angioplasty was performed in one patient,the others were treated with Wallstent stents after PTA.Result:Immediately after treatment,angiography and ultrasound showed that the proportion of stenosis was zero and all appeared to achieve in good result.The artery treated with PTA was completely occluded after 1 year follow-up;the other two patients with stents placement were also examined by angiography and ultrasound at 4,5 months and 4 months respectively after the praeedure.For the patient with left carotid artery stenosis,the proximal part of the segment with the intravascular stent showed a restenosis;the other patient was normal.Conclusion:We considered that intravascular stent placement be might to the treatment for Takayacu' s arteritis with long segment stenosis of common carotid artery.

0.05).Significant difference existed in the coliform qualification rate in the source water samples taken at various times (P0.05).However,significant difference existed in qualification rate of bacteria,fecal coliform,free chlorine residual (P

Objective:To examine the changes of matrix metalloprotein as e-2 (MMP-2) and tissue inhibitors of matrix metalloproteinase-2(TIMP-2) in g ingival crevicular fluid(GCF) after periodontal initial treatment of adult perio dontitis pateins. Methods:42 teeth of 42 adult periodontitis pat eins and 42 teeth of 42 periodontal healthy subjects were included.GCF was samp led with filter paper strips by intra-pocket methed. Assays for MMP-2 and TIM P-2 in GCF were performd by ELISA.Results:Levels of MMP-2 and TIMP-2 were higher in patients than that in the controls(P

Objective To explore the extent and severity of cognitive dysfunction in patients with schizophrenia and in order to offer more credible evidence to the treatment of schizophrenia. Methods Eighty-eight patients with schizophrenia and Fifty healthy controls in Shaoxing city were measured with Wechsler Memory Scale (WMS-RC), Wechsler Adult Intelligence Scale (WAIS-RC), Cancellation Test (CT) and Wisconsin Card Sorting Test (WCST). Results There was statistically significant difference between the two groups in visual recognition, visual reproduction, 1→100, 100→1, accumulation, picture remember, associative learning, touch test, logical memory, reciting figure and memory quotient of WMS-RC (t=2.40, 4.66, 2.90, 3.48, 4.03, 4.40, 2.13, 3.01, 3.97, 2.29, 4.87, respectively; P