1.Effect of tripterygium glycosides on serum ion and β2-GPI/ox-LDL in patients with IgA nephropathy
Chinese Journal of Biochemical Pharmaceutics 2015;(11):139-141
Objective To analyse effect of tripterygium glycosides on serum ion and β2-GPI/ox-LDL in patients with IgA nephropathy. Methods 54 patients who were diagnosed with primary IgA nephropathy in our hospital were collected.All patients were randomly divided into experimental group and control group, 27 cases in each group.Control group was given conventional Benazepri1 Hydrochloride Tablets 10 mg, 1 times per day orally;given Dipyridamole Tablets 50 mg, 3 times per day orally, patients in experimental group were given tripterygium glycosides 20 mg on the basis of control group treatment orally,3 times per day.All the patients were treated for 6 month.After the treatment, the serum levels of Ca,P,β2-GPI/ox-LDL and renal function related index were detected in all patients.Results Compared with control group post-treatment, the serum level of Ca was higher(P<0.05); and the serum level of P was lower in experimental group(P<0.05); the serum level of β2-GPI/ox-LDL was lower in experimental group(P<0.05);the serum levels of Scr and BUN were lower in experimental group(P<0.05).Conclusion The tripterygium glycosides can significantly reduce the serum levels of P,β2-GPI/ox-LDL,Scr and BUN,and increase serum Ca level in patients with IgA nephropathy, improve renal function.
2.Study of the relationship between nicotinamide adenosine dinucleotide phosphate oxidase in peripheral blood mononuclear cell and left ventricular remodeling in patients with acute myocardial infarction
Jianyun LIU ; Peng XU ; Xun LI
Chinese Journal of Postgraduates of Medicine 2012;35(19):4-6
Objective To investigate the relationship between the activity of nicotinamide adenosine dinucleotide phosphate(NADPH ) oxidase in peripheral blood mononuclear cell and left ventricular remodeling in patients with acute myocardial infarction (AMI).Methods Fifty-two patients with AMI hospitalized from December 2010 to June 2011 were selected as AMI group,and 25 patients with paroxysmal supraventricular tachycardia hospitalized in the same period were selected as control group.All patients were examined by Doppler echocardiography and left ventricular mass index (LVMI) was calculated.Fasting blood was drawn on the second day after hospitalization to measure the level of biochemical indexes,malondialdehyde(MDA ) and the activity of NADPH oxidase.The correlation of the level of MDA,the activity of NADPH oxidase and LVMI was analyzed.Results The level of MDA in AMI group was significantly higher than that in control group [(6.14 ±t 2.80) μ mol/L vs(2.95 ± 1.82) μmol/L,P < 0.01 ].The levd of O2- in AMI group was also obviously higher than that in control group [(21.61 ± 10.66) RLU/mg protein vs.( 7.12 ± 4.48 ) RLU/mg protein,P < 0.01 ],as well as the activity of NADPH oxidase [ ( 2.84 ± 1.05 ) RLU/mgprotein vs.( 1.97 ± 0.80) RLU/mg protein,P < 0.01].Positive correlation was found between LVMI and the level of MDA(r =0.57,P < 0.01 ),as well as between LVMI and the activity of NADPH oxidase(r =0.71,P<0.01 ).Conclusions The activity of NADPH oxidase in peripheral blood mononuclear cell inereases in AMI patients and plays an important role in left ventricular remodeling after AMI.
3.Specific anti-tumor immune responses of dendritic cells pulsed with recombinant human rhHSP70 and freeze-thaw cellular lysates derived from breast cancer
Bin LI ; Peng CHEN ; Jianyun ZHENG
Chinese Journal of Clinical Oncology 2013;(15):888-892
Objective:This work aims to use the characteristics of dendritic cells (DCs) pulsed with recombinant human HSP70, which can present and process tumor antigens, to enhance the killing activity of cytotoxic t lymphocytes (CTLs) against breast neoplasms. Methods:Autologous DCs were isolated from peripheral blood mononuclear cells and then stimulated in vitro with granulocyte macrophage-colony stimulating factor and interleukin-4. The DCs were loaded with A549 tumor cell freeze-thaw lysate, and rhHSP70 was added as an immune adjuvant. The specific groups were subjected to tumor-specific cytotoxic assay, enzyme-linked immunosorbent assay, and fluores-cence-activated cell sorting. Results:DCs pulsed with A549 tumor cell lysate enhanced the growth expansion of CTLs, upregulated CD40 and CD80 populations in CTLs, and augmented Th1 cytokines. In addition, the cytotoxicity of specific CTLs against A549 was highly enhanced. The above indications became more obvious after the addition of rhHSP70. Conclusion:DCs pulsed with freeze-thaw cell lysates derived from breast cancer can enhance growth expansion of lymphocytes. They may serve as an effective tumor antigen to stimulate the proliferation of specific CTLs, which are very effective in activating specific T-cell responses against breast cancer cells in vitro. The improved anti-tumor immunity response by DC-based vaccines may be related to the maturation of the DCs promoted by rhHSP70.
4.Analysis of influencing factors on hematuria in patients with acute coronary syndrome
Peng XU ; Jianyun LIU ; Xun LI
Chinese Journal of Postgraduates of Medicine 2012;35(10):4-6
ObjectiveTo investigate the impact of blood glucose,blood pressure and blood uric acid level on hematuria in patients with acute coronary syndrome (ACS).MethodsOne hundred and sixty-two ACS patients were selected and received standardized treatment after admission to hospital.Urine test was taken and patients were divided into no hematuria group(37 cases),microscopic hematuria group (56 cases) and gross hematuria group(69 cases) according to the results.Blood pressure,fasting and postprandial 2 hours blood glucose,glycated hemoglobin and blood uric acid level were measured and compared among three groups.ResultsMicroscopic hematuria group compared with no hematuria group,fasting and postprandial 2 hours blood glucose,glycated hemoglobin,systolic blood pressure and blood uric acid level raised 58.0%[(7.9 ±0.7) mmol/L vs. (5.0 ± 1.1) mmol/L],33.3%[(12.4 ±0.8) mmol/L vs.(9.3 ± 0.6 ) mmol/L ],48.2% [ ( 8.3 ± 0.8 )% vs.( 5.6 ± 0.5 )% ],23.8% [ ( 151.6 ± 7.0) mm Hg ( 1 mm Hg =0.133 kPa) vs.(122.5 ±9.9) mm Hg],29.2% [(635.4 ±47.4) μmol/L vs.(491.8 ±83.4)μmol/L]respectively,there were significant differences (P < 0.05 or < 0.01 ).Gross hematuria group compared with microscopic hematuria group,the above mentioned indexes raised 16.5%[ (9.2 ± 1.1 ) mmol/L vs.(7.9 ± 0.7)mmol/L],30.6%[ ( 16.2 ± 1.8) mmol/L vs.( 12.4 ± 0.8) mmol/L],14.5%[ (9.5 ± 0.8)% vs.(8.3 ± 0.8)% ],18.8%[(180.1 ± 12.3) mm Hg vs.(151.6 ±7.0) mm Hg],34.6%[(855.5 ±74.5) μ mol/L vs.(635.4 ±47.4 ) μ mol/L ] respectively,there were significant differences ( P < 0.05 or < 0.01 ).Gross hematuria group compared with no hematuria group,the above mentioned indexes increased significantly(P< 0.01 ).The level of diastolic blood pressureamong three groups had no significant difference(P > 0.05).The Logistic regression analysis showed that fasting and postprandial 2 hours blood glucose (r =3.175,P =0.001 ;r =0.906,P =0.001 ),glycated hemoglobin ( r =16.109,P =0.001 ),systolic blood pressure (r =0.429,P =0.003 ),blood uric acid level(r =1.317,P =0.004) were risk factors on hematuria after antiplatelet and anticoagulant therapy in ACS patients,the impact of glycated hemoglobin and fasting blood glucose on hematuria was stronger than that of blood uric acid level and systolic blood pressure.ConclusionWith the increase of blood glucose,systolic blood pressure,blood uric acid,the risk of hematuria increases in ACS patients.
5.Dendritic Cells Pulsed with Recombinant Human Hsp70 and Mage-3 Peptide from Breast Cancer Induce Specific Anti-tumor Immune Responses
Bin LI ; Peng CHEN ; Jianyun ZHENG
Journal of Kunming Medical University 2013;(8):36-39
Objective To make use of the characteristics of presenting and processing tumor antigen of Dendritic cells pulsed with recombinant human Hsp70 and Mage-3 peptide to enhance killing capability of breast neoplasms. Methods Autologous dendritic cells were isolated from PBMC and then stimuiated in vitro with GM-CSF and IL-4.Dendritic cells were loaded with Mage-3 peptide;at the same time Hsp70L1 was added. The specific groupings each induce generation of tumor specific cytotoxic assay with ELISA. Results DCs pulsed with Mage-3 peptide enhanced the growth expansion of CTL, and promoted the secretion of cytokines such as TNF-αand IL-6. DCs pulsed with recombinant human Hsp70 and Mage-3 peptide had cytotoxicity against human breast cancer cells MCF-7.Conclusions DCs pulsed with recombinant human Hsp70 and Mage -3 peptide have higher biological activities. Modified DCs can stimulate the proliferation of lymphoeytes,present effectively tumor antigen to stimulate generation of speeific CTLs. Dendritic cells pulsed with recombinant human Hsp70 and Mage-3 peptide have high ability to kill breast cancer cells.
6.Evaluation of ~(18)F-FDG hPET/CT in primary carcinoma of gastric cardia
Jianyun WANG ; Kangsheng SONG ; Wenjun JU ; Shouxing PENG ; Wenrui ZHANG
China Oncology 2001;0(03):-
Background and Purpose:dPET/CT is very expensive,so although the accuracy of hPET/CT is less than dPET/CT,it is better than CT,and is useful for guiding the tumor staging before operation.This paper is evaluate the role of ~(18)F-fluorodeoxyglucose(FDG) hPET/CT in primary carcinoma of gastric cardia detection.Methods:26 patients with histologically proven primary carcinoma of gastric cardia underwent whole body hPET/CT imaging.Visual and semiquantitative analysis and standardized uptake value(SUV) were used to analyze the images.The results of hPET/CT study were compared with those of CT.Results:1.The sensitivity of hPET/CT in primary carcinoma of gastric cardia detection was 92.3%(24/26);two cases of signet ring cell carcinoma gave false negative results,and the primary lesions were small(
7.Surgical treatment and perioperative period management for elderly lung cancer patients
Jianyun WANG ; Shouxin PENG ; Zheng RUAN ; Al ET
China Oncology 2001;0(05):-
Purpose:To explore the problems in the treatment of elderly patients suffering from lung cancer. Methods:58 cases of elderly patients aged over 70 who received operation for lung cancer during Oct. 1997 to Aug. 2002 were analyzed retrospectively.Results:56 cases underwent resections (resection rate 96.6%), of which 48 cases (82.8%) had radical resection and 8 cases (13.8%) palliative resection.2 cases (3.4%) had exploratory surgery. Postoperative complications were found in 50 cases (86.2%), of which 2 cases died during perioperation period(mortality rate 3.4%). Conclusions:Old age is not the major determining factor when considering surgical operation as the treatment for lung cancer patients. In order to enhance resection rates especially radical resection rate and survival rate, to diminish postoperative complications and mortality, and thus to improve the quality of life for the elderly lung cancer patients, a series of measures must be taken. First, diagnosis and treatment of the associated condition must be emphasized. Second, full preoperative preparations with appropriate choice of the time and procedure for operation. Finally, intensive monitoring during the preoperative period and prevention and treatment of postoperative complications are also important factors to be considered.
8.Synthesis and activity evaluation of novel tyrosine kinase inhibitors
Hongpeng YANG ; Gang WANG ; Tao PENG ; Xiaoxue WEN ; Jianyun YANG ; Yunbo SUN ; Shuchen LIU ; Shouguo ZHANG ; Lin WANG
Journal of International Pharmaceutical Research 2017;44(6):575-579
Objective To design and synthesize compounds with protein tyrosine kinase(PTK)inhibitory activity with L029 as the lead compound. Methods L029 derivatives were designed and synthesized from L029 by reduction and/or substitution with the 3-dimethylamino-1-propyl,methyl acetate,methyl propionate in its active H and other sites. PTK activity was measured by enzyme-linked immunosorbent assay(ELISA). The inhibitory rate was calculated to screen out the compounds with PTK inhibitory activity. Re-sults Five target compounds were synthesized and their structures were confirmed by 1H NMR and MS. Three compounds T2,T3 and T5 were screened out with strong PTK inhibitory activity. Conclusion The synthetic routes of the target compounds are simple with mild reaction condition,and 3 compounds show strong inhibitory activity by ELISA. These results can provide reference for the further design and synthesis of this kind of molecules.
9. Study on risk factors of catheter-related venous thrombosis and prevention effect of low-molecular-weight heparin in patients with hematological malignancies
Jianyun LI ; Chuanqing TU ; Ling PENG ; Can HUANG ; Xuyan ZHANG ; Dianwen WANG ; Caifeng ZHENG
Cancer Research and Clinic 2019;31(10):679-683
Objective:
To investigate the incidence and risk factors of catheter-related venous thrombosis (PICC-DVT) after peripherally inserted central catheter (PICC) in patients with hematologic malignancies, and to analyze the safety of anti-coagulation therapy with low-molecular-weight heparin.
Methods:
From August 2016 to June 2018, 43 patients with hematologic malignancies received PICC in Baoan District People's Hospital of Shenzhen City were enrolled. The patients were divided into low-molecular-weight heparin anticoagulation group (22 cases) and blank control group (21 cases) according to the random number table method. The blood routine, coagulation quadruple, D-dimer, protein C activity, protein S activity, and antithrombin Ⅲ activity before and after catheterization were compared between the two groups.
Results:
Of the 43 patients, 5 cases (11.62%) occurred PICC-DVT within 1 month after PICC, including 2 cases (9.09%) in the low-molecular-weight heparin anticoagulation group, and 3 cases (14.29%) in the blank control group, the difference between the two groups was not statistically significant (
10.Dynamic expressions of miR-34a and related target genes sirtuin 1 and nuclear factor-E2-related factor 2 and their significances in subependymal zone of rats with cerebral ischemia
Wei LIAO ; Leiying ZHANG ; Peng LIU ; Liansheng ZOU ; Jianyun LOU ; Shaochun YANG
Chinese Journal of Neuromedicine 2019;18(5):447-452
Objective To observe the expression changes ofmiR-34a and its related target genes sirtuin 1 (Sirtl) and nuclear factor-E2-related factor 2 (Nrf2) in subependymal zone (SVZ) of rats after cerebral ischemia,and to explore the effect of miR-34a on proliferation of endogenous neural stem cells (NSCs) after cerebral ischemia.Methods Forty SD rats were randomly divided into control group,cerebral ischemia one d group,cerebral ischemia three d group and cerebral ischemia 7 d group (n=10).Middle cerebral artery occlusion models were established by thread embolization in the latter three groups,and the SVZs were taken one,three and 7 d after model making,respectively.Immunofluorescence staining was used to detect the expression of nestin,real-time PCR was used to detect the mRNA expressions ofmiR-34a,Sirtl and Nrf2,and Western blotting was used to detect the protein expressions of Sirt1 and Nrf2.Results (1) In SVZs of cerebral ischemia one d group,cerebral ischemia three d group and cerebral ischemia 7 d group,the number of nestin positive cells increased gradually ([2.013±0.526],[4.821 ±1.154],and [6.394±1.027]) cells/filed,with statistical differences (P<0.05).(2) In SVZs of cerebral ischemia one d group,cerebral ischemia three d group and cerebral ischemia 7 d group,the expression levels of miR-34a mRNA increased gradually (0.295±0.145,0.701 ±0.075,and 1.136±0.018),the Sirt1 mRNA expression levels showed a downward trend (0.835±0.024,0.620±0.133,and 0.278±0.110),the Nrf2 mRNA expression levels obviously decreased (1.032±0.256,0.833±0.187,and 0.630±0.123),the Sirt1 protein expression levels showed a downward trend (0.832±0.018,0.731±0.156,and 0.454±0.132),and the Nrf2 protein expression levels obviously decreased (1.003±0.133,0.813±0.111,and 0.671 ±0.071),with statistically significant differences (P<0.05).(3)Statistical correlation analysis showed that the number of nestin positive cells was positively correlated with miR-34a mRNA expression level and negatively correlated with Sirt1 and Nrf2 mRNA and protein expression levels after cerebral ischemia (r=0.887,P=0.003;r=-0.746,P=0.005;r=-0.521,P=0.013;r=-0.344,P=0.025;r=-0.863,P=0.000).Conclusions MiR-34a may regulate the proliferation of NSCs through Sirt1/Nrf2 pathway after cerebral ischemia.