Objective To study the metabolic profiles in the urine samples of rats dosed with decoction of the caulis of Aristolochia manshuriensis and the relationship between the results of blood biochemical analysis and histopathological analysis,and to explore the feasibility of the application of NMR-based metabonomics to toxicology of Chinese medicine.Methods Male Wistar rats were administrated by ig 36,32,28,and 24 g/kg of A.manshuriensis or the equal volume of distilled water for 6 d,respectively.Urine were collected and their()~1H-NMR spectra were acquired,and then subjected to data process and principal component ana-(lysis)(PCA).Blood biochemical analysis and histopathological examination were carried out.Results(Compared) to control groups,the urinary concentrations of TMAO,taurine,creatinine,DMG,citrate,and DMA in dosed groups decreased,while those of acetate,alanine,and some other amino acids increased to a great extent.The dosed groups can be readily discriminated from the controls based on PCA,with acetate and TMAO as main discriminators.A good agreement was achieved within clinical chemistry,microscopically examination and PCA data.Conclusion The caulis of A.manshuriensis can induce renal lesion as revealed by the metabonomic analysis of rat urine samples as well as blood biochemical analysis and histopathological examinations.The results show that the metabonomic approach is a promising new technology in toxicology.

ve To evaluate the performance of the target controlled infusion (TCI) system of midazolam for sedation during operation in terms of safety and ease of use in the elderly and the young. Methods Forty-four unpremedicated ASA I - II patients scheduled for elective surgery under epidural or combined spinal-epidural (CSE) anesthesia were included in this study. The patients were divided into two age groups: the elderly group aged 61-82 yr ( n = 22) and the young group aged 19-43 yr (n = 22) . Patients with neurological or severe cardiopulmonary diseases or abnormal liver or kidney function were excluded. The block height was maintained below T4. The TCI system consisted of Intel Pentium III 450 MHz computer and Graseby 3500 computerized infusion pump. The software we used was Stelpump Version 1.05 written by Pina and Coetzee and the pharmacokinetic model and parameter were described by Arram and Buhrer. The effect site concentration of midazolam was targeted and started from 50ng?ml-1, then increased in increment of 50 ng?ml-1 (in the young group) or 25ng?ml-1 (in the elderly group) until the patient did not respond to light shaking (defined as unconsciousness) . Each incremental increase was maintained for 15 min. Arterial blood samples were taken in ten patients randomly selected from each group at 10 and 15min after each incremental increase of midazolam concentration for determination of plasma midazolam concentration by high performance liquid chromatography ( HPLC) .Results The bias (median performance error) of the TCI system of midazolam was 7.9% in the elderly group and 11.6% in young groups. The precision (median absolute performance error) was 20.0% in both groups. They were clinically acceptable. Conclusions The TCI system of midzolam can provided sedation for patients undergoing surgery under spinal and/or epidural anesthesia safely and efficiently in both the old and young.

Objective To assess the changes in heart rate variability (HRV) during midazolam sedation with TCI in the elderly and young patients. Methods Thirty-eight ASA I - Ⅱ patients scheduled for elective surgery on lower abdomen or lower extremities under epidural or combined epidural-spinal anesthesia were divided into two groups of 19 patients each: the young group (18-40 yr) and the elderly group (60-89 yr). Radial artery was cannulated for intra-arterial pressure monitoring and blood sampling. HRV was monitored by HXD-1 monitoring system. HRV parameters included total power (TP), low frequency(LF), high frequency(HF), LF/HF, nuLF(LF/TP ?100%) and NuHF(HF/TP ? 100%) . The height of block was maintained below T5. TCI was used to achieve a rapid induction and maintenance of a stable target blood midazolam concentration. Target blood midazolam concentration was started from 50 ng/ml and gradually increased with increment of 25 ng/ml in elderly group or 50ng/ml in the young group until loss of consciousness (OAA/S =1). Arterial blood samples were taken at each target blood midazolam concentration for determination of blood midazolam concentration. HRV parameters were recorded at different OAA/S scores during both the induction of and recovery from sedation. Results With increasing depth of sedation, most of the HRV parameters (LF, nuLF, LF/HF and TP) decreased progressively, while nuHF increased and HF remained unchanged. During recovery from midazolam-induced sedation, HRV parameters returned gradually to the baseline values. OAA/S scores and HRV parameters were well correlated(r = 0.502-0.719). The trend of changes in HRV was consistent in both groups. At the same OAA/S score, most values of HRV parameters were not significantly different between the two groups. Only during deep sedation (OAA/S=1), values of all HRV parameters were significantly lower in the elderly group than those in the young group, except LF/HF. Conclusions During profound sedation (OAA/S=1) midazolam depresses the activity of the autonomic nervous system more markedly in the elderly than in the young, but the elderly without cardiovascular and autonomic nervous system disorders has the same ability to keep the balance between the sympathetic and parasympathetic nervous system as theyoung.

0.05).A similar amount of propofol was used in each group.A significantly higher dose of sufentanil was administered in the Group C(73.9?13.6 ?g)than in the Group A(24.3?4.9 ?g)and the Group B(35.4?8.1?g)(qC-A=237.924,P=0.000;qC-B=119.385,P=0.000).The expected time of recovering consciousness was shorter in the Group A and B than in the Group C.Conclusions Administered by target-controlled infusion with propofol,sufentanil dose-dependently reduces BIS and modifies hemodynamic responses during anesthesia induction.The target effect-site sufentanil concentration of 0.6 ng/ml combined with plasma propofol concentration of 3 ?g/ml is suitable for anesthesia induction for short-time laparoscopic operation.

0.05). The requirement of vasoactive drugs in the group A was significantly smaller than that in the group B (P

At present,the medical profession generally acknowledged the best way to treat osteosarcoma is gene therapy,which includes tumor suppressor gene therapy,antisense gene therapy,suicide gene therapy,immune gene therapy,combined gene therapy,etc.But no matter what kind of gene therapy is that the gene must have a safe carrier.Gene therapy has made a breakthrough in osteosarcoma recently.On the basis of widespread use,we should emphasize the importance of gene vectors.

To investigate the protective effects of the n-butanol extract of Potentilla anserina L. (NP) on pituitrin-induced acute myocardial ischemic injury in mice.

Objective To evaluate the differences of serum RANTES(regulated on activation, normal T cell expressed and secreted), MCP-1 (monocyte chemoattractant protein), and SDF-1β (stromal cell-derived factor-1β) in patients with acquired immunodeficiency syndrome (AIDS) and healthy people.Methods 38 AIDS patients who were admitted to a hospital between January 2010 and January 2015 were as AIDS groups, 38 healthy persons were as a healthy group, serum levels of RANTES, MCP-1, and SDF-1β in two groups were detected, and the subgroup analysis was carried out according to the viral load.Results Serum levels of RANTES, MCP-1, and SDF-1β in AIDS group were (1 392.55±227.69)pg/mL,(450.91±103.04)pg/mL, and(104.82±22.52)pg/mL respectively,all were significantly higher than those in healthy group([120.58±55.87] pg/mL, [74.25±33.62] pg/mL, and [39.04±11.43]pg/mL respectively)(all P<0.05).Among AIDS patients with HIV viral load 4≤Log(VL)<5 and Log(VL)≥5, serum RANTES were (1 470.34±155.01)pg/mL and (1 408.29±181.54)pg/mL respectively,which were both significantly higher than patients with HIV viral load Log(VL)<4([1 183.12±174.54]pg/mL);serum MCP-1 and SDF-1β levels in AIDS patients with HIV viral load 4≤Log (VL)<5 were (537.93±89.32)and(149.31±18.05)pg/mL respectively,which were significantly higher than patients with HIV viral load Log(VL)≥5([410.26±80.57] pg/mL, [81.53±20.31]pg/mL respectively) and HIV viral load Log(VL)<4([381.71±77.26] pg/mL, [72.90±21.62]pg/mL respectively), differences were both statistically significant(both P<0.05).Conclusion Serum levels of RANTES, MCP-1, and SDF-1β are significantly increased in AIDS patients, which are related to the level of viral load.

ObjectiveTo evaluate the clinical and functional outcome of traumatic segmental defects of the tibia treated by internal bone transport using the the adjustable mono-lateral external fixation.Methods12 patients who underwent internal bone transport using the the adjustable mono-lateral external fixation for traumatic segmental defects in the tibia were reviewed, the length of bone defect was 6～10 cm.ResultsMean follow-up was 14 months. All the cases got primary bone union. The length of time in healing was 9～14 months.ConclusionTraumatic segmental defects of the tibia treated by internal bone transport using the adjustable mono-lateral external fixation is a simple, less traumatic, non-bone graft and effective way.

BACKGROUND: Three kinds of donor meniscus are commonly used at present, namely cryopreserved, fresh and deep-frozen meniscus, which,however, almost invariably give rise to degenerative changes of various degrees after transplantation.OBJECTIVE: To compare the outcome of transplantation of allograft deep-frozen meniscus and meniscal acellular matrix to determine the most preferable means of allograft meniscus preservation.DESIGN: Randomized controlled experiment with rabbits.SETTING: Department of Orthopedics, Second Hospital Affiliated to Harbin Medical University.MATERIALS: Sixty-four male Japanese white rabbits with body mass of 3.0 - 3.5 kg.METHODS: This experiment was carried out in the Animal Experimental Center, Second Hospital Affiliated to Harbin Medical University between September 2002 and September 2003. Totally 64 adult rabbits were assigned into 32 pairs according to the body weight to served as the donor and the recipient animals, respectively. The medial menisci was obtained from the bilateral knees of the donor animals with the right one cryopreserved at -80 ℃ and the left prepared into acellular matrix for deep-frozen preservation. The donor menisci were respectively transplanted into the corresponding knee joints of the recipient animal's hindlimbs, with the left side taken as the experimental side and right as control. Gross observation,X-ray examination, and histological examination of the tissues were carried out at postoperative 4, 8, 12 and 16 weeks, respectively.MAIN OUTCOME MEASURES: Findings in X-ray, gross observation and histological observation of the grafted meniscus with meniscal measurement and findings in abdominal aorta perfusion.RFSULTS: All the 64 rabbits were observed for result analysis. X-ray examination of the grafted meniscus revealed no obvious changes in either the experimental and control side at 4 and 8 weeks postoperatively, but mild changes occurred on the control side at 12 weeks, which became obvious at 16 weeks, presented by joint space narrowing, hyperostosis and osteosclerosis below the cartilage of varied severities (with scores of 1.3 and 0.6, respectively, P ＜ 0.05). By gross observation, meniscal atrophy on the experimental side was milder and slower than the control side, with al so lower atrophy rate [(15.14±4.62) % vs (20.97±4.72) % at week 4, P ＜ 0.001, and (19.23±11.27) % vs (32.74±10.43) % at week 16, P ＜ 0.05].Perfusion of the abdominal aorta revealed no revascularization in the surrounding tissues of the meniscus by gross observation in either groups, but histologically, the experimental side showed more favorable structure than the control side at postoperative weeks 4, 8, 12 and 16.CONCLUSION: Meniscal acellular matrix may produce better outcome than deep-frozen meniscus after transplantation and can be a more practical means for preservation of the meniscus.