In light of the idea of realizing management by capital, the authors expound the theoretical basis of implementing the integration of the capital of funds and the capital of knowledge and give an account of their hospital's experience in this aspect. By introducing capital and talented personnel, viz. by liquidating remnant assets through the capital of knowledge and by seeking benefits through capital integration, the hospital has succeeded in inventing a new way of utilizing talented personnel resources and stimulating talented personnel to greater efforts, thus enhancing the speed of its accumulation of capital and laying a solid foundation for sustainable development.

In light of their hospital's practice in adopting new operational ideas and management models for the past three years, the authors expound the content of the new operational ideas, the necessity of applying these new ideas to hospital management, and the four basic principles that must be followed in constructing new management models. They also discuss some key issues involved in the process of the hospital's construction of new management models.

Objective To investigate the relationships between concentrations of free fatty acid (FFA) in maternal serum and oxidative damage levels in placental mitochondria and preeclampsia ( PE)-Methods A total of 60 women with PE and 60 normal pregnant women as control participated in this study.All were admitted to Fujian Maternity and Child Health Hospital for delivery from August 2010 to May 2011.Patients with PE were divided into early-onset group ( n =30,presented at ＜ 34 weeks of gestation ) and late-onset group ( n =30,presented at ≥ 34 weeks of gestation),with 30 normal pregnant women as early control group ( ＜ 34 weeks of gestation ) and 30 as late control group ( ≥34 weeks of gestation).Improved copper agent colorimetry was used to detect FFA in maternal serum Ultraviolet colorimetry was used to detect glutathione peroxidase (GPX) and catalase (CAT) activity in maternal placenta and malondialdebyde (MDA) and permeability transiton (PT) pore in placental mitochondria.Total superoxide dismutase (SOD) assay kit-WST was used to detect SOD activity in placenta.Real-time fluorescent quantitative PCR was used to detect mitochondrial DNA (mtDNA) expression in placenta.Results ( 1 ) Maternal serum FFA was ( 1.6 ±0.5 ) mmol/L in early-onset PE group and ( 1.5 ± 0.4) mmol/L in lateonset PE group,significantly elevated as compared to ( 1.0 ± 0.5 ) mmol/L in early control group and (0.9 ±0.5) mmol/L in late control group (P ＜ 0.05 ). However,no significant difference was found between early-onset and late-onset PE groups (P ＞ 0.05 ).(2) The mean placental GPX,CAT and SOD activity were significantly decreased in the early-onset PE group [ (47 ±6),( 19 ±5),(62 ± 13) U/mg]and late-onset PE group [ (67 ±6),(20 ±4),(96 ± 17) U/mg] as compared to late control group [ (80 ±3),(55 ± 3 ),( 123 ± 19 ) U/mg],respectively ( P ＜ 0.05 ).(3) The mean placental mitochondria MDA was significantly elevated in the early-onset PE group [ (115 ± 22) nmol/mg] and late-onset PE group [(90±17) nmol/mg] as compared to late control group [(52 ± 11) nmol/mg,P ＜0.05].The mean absorption value that present the permeability of placental mitochondria PT pore was significantly elevated in the early-onset PE group (0.086 ±0.013) and late-onset PE group (0.069 ±0.014) as compared to late control group (0.052 ± 0.0 12,P ＜ 0.05 ).The mean placental mtDNA expression was significantly elevated in the early-onset PE group (3.0 ±0.7) and late-onset PE group (2.8 ±0.7) as compared to late control group ( 2.6 ± 0.6,P ＜ 0.05 ).( 4 ) The mean placental mitochondria MDA concentration correlated positively with the concentrations of FFA in maternal serum in the early-onset PE group ( r =0.703,P ＜0.05 ) and late-onset PE group (r =0.457,P ＜ 0.05 ),and negatively with placental antioxidant enzyme in the early-onset PE group ( r =- 0.652,- 0.787,- 0.952 ; P ＜ 0.05 ) and late-onset PE group ( r =-0.378,-0.689,-0.854; P＜0.05).Conclusions Increased FFA in maternal serum and high levels of oxidative damage in placental mitochondria may be involved in the pathogenesis of preeclampsia.Increased FFA in serum and decreased activity of antioxidant enzyme in placenta may contribute to oxidative damage levels in placental mitochondria in women with PE.

Objective To explore the anti-inflammatory therapeutic role of erythromycin in chronic obstructive pulmonary disease(COPD) by investigating the effect of erythromycin on the level of nuclear factor kappa B(NF-?B) and intercellular adhesion molecule-1(ICAM-1) in airway epithelium of smoking rats.Methods Wistar rats(n=30)with weighed(150?10) g each,20 were randomly sampled to be exposed to cigarettes smoking for four weeks then 10 of them were exposed wntinuously to cigaretts smoking for eight weeks as smoking,group,other loof them were intragastrically perfused by erythromycih before smoking as evythromycin group,the rest of total 30 rats as control group Then assessed expiratory airway resistance and lung compliance and observed the expression level of NF-?B and ICAM-1 in airway epithelium by using immunohistochemical method.Results(1) The expiratory airway resistance was increased and the lung compliance was degraded significantly in smoking group(5.67?0.32,4.39?0.40)(P

ObjectiveTo investigate the effects of nicotine on the expression of Th1/Th2 cytokines and transcription factor T-bet/GATA-3 in cultured CD4+ T of rat sensitized by ovalbumin.Methods Two weeks after immunization by ovalbumin,splenic CD4+ T cells of Wistar rat were purified using CD4+T cell enrichment kit.Purified CD4+ T cells of rat were cultured and divided into 4 groups:a control group,1 μg/ml nicotine stimulated group,10 μg/ml nicotine stimulated group,100 μg/ml nicotine stimulated group.These cells,in their groups,were stimulated with or without nicotine and were all challenged simultaneously with OVA.Supernatants and cell pellets were harvested after being stimulated for 24 h.The concentration of IFN-γ and IL-4 in supernatants were measured by enzyme-linked immunosorbent assay (ELISA).Real-time PCR was used to determine the mRNA expression of T-bet and GATA-3 in CD4+T cells.Results ( 1 ) IFN-γproduction was significantly decreased in all nicotine treated groups [ ( 113.78±6.06) ng/L,(70.31±7.26) ng/L,(20.00±2.14) ng/L] compared with the control group[ (142.30± 5.89) ng/L],and the level of IL-4 was significantly increased in all nicotine treated groups [ (69.49±3.91) ng/L,(93.63±4.56) ng/L,(50.97±3.07) ng/L] compared with the control group[ (36.91±3.24) ng/L].(2) Expression of T-bet mRNA in all nicotine treated groups(0.73±0.03,0.57±0.04,0.31 ±0.00) was lower than that in the control group(0.98±0.09),but expression of GATA-3 mRNA in all nicotine treated groups (4.31±0.26,5.16±0.23,1.56±0.14) was significantly higher than that in the control group(1.00±0.07).Conclusion Nicotine may play a key role in the development of Th2-type allergic inflammation in asthma by promoting over-expression of GATA-3 mRNA and downregulating the expression of T-bet mRNA.

Objective To measure circulating vascular endothelial growth factor-C(VEGF-C) levels in(patients) with colorectal carcinoma,and assess the clinical significance in diagnosis of lymph node metastasis.Methods Sixty-six patients with colorectal carcinoma and 30 healthy control patients were included in this study.Circulating VEGF-C and VEGF levels were assessed by enzyme-linked immunosorbent assay.Results Serum VEGF-C and VEGF concentration was higher in patients with colorectal carcinoma than in healthy control patients(P

ObjectiveTo explore the influence of the cigarette smoke in the airway inflammation and pulmonary function of the asthmatic patients. MethodsTwenty-five cases of asthmatic patients with cigarette smoke exposure, 22 cases of asthmatic patients without cigarette smoke exposure and 20 cases of normal control persons were involved in this study. The proportion of various inflammatory cells in the induced sputum, the levels of serum interleukin (IL)-8 and IL-4 and lung function (FEV1％ expected value,FEV1/ＦVC％ ) were detected. ResultsThe infiltrating of neutrophils was primarily found in sputum of the asthmatic patients with cigarette smoke exposure, but the infiltrating of eosinophils was mainly in sputum of the asthmatic patients without cigarette smoke exposure. The levels of serum IL-8 and IL-4 of peripheral blood of asthmatic patients with cigarette smoke exposure[(277.02 ±71.37), (171.69 ±31.01) ng/L] were significantly higher than those in asthmatic patients without cigarette smoke exposure[(158.88 ± 21.95 ),( 111.42 ± 21.69 ) ng/L] and normal control persons [( 116.78 ± 71.37 ), (73.94 ± 15.72 ) ng/L] (P ＜ 0.01 ).The FEV1％ expected value and FEV1/FVC％ of the asthmatic patients with cigarette smoke exposure [(51.12 ± 13.30) ％, ( 49.16 ± 11.09 )％] was lower than those of asthmatic patients without cigarette smokeexposure [(81.81 ± 5.82)％, (79.00 ± 3.86)％] and normal control persona [(95.50 ± 10.11 )％, (83.18 ±6.04)％] (P ＜ 0.01 ). The level of serum IL-8 was positively correlated to the neutrophils percentage in the induced sputum (r =0.742,P＜ 0.01 ) ,while negatively correlated to the FEV1％ expected value(r =-0.739,P ＜ 0.01 ). ConclusionCigarette smoke may influence the airway inflammation of the asthmatic patients and accelerate the deterioration of their lung function by promoting the producing of IL-8.

Objective To evaluate the effect of the activity of Ca2+/CaN-NFATc on the activation and prolfferation of lymphocyte in asthmatic rats.Methods The rats of the asthma group and the CsA group were sensitized and challenged by ovalbumin.So did the control group with saline instead.Lymphocyte was separated from spleen and cultured for 24 hours,and PHA-p (5 μg/ml) was added to the culture medium in every group,CsA ( 1.0 μg/ml) was added to the CsA group,respectively.The concentration of [ Ca2+ ] i,the activity of CaN,the protein expression of dephosphorylated NFATc and Cyclin E in T lymphocyte were assayed.The level of IL-4 and IL-2 in culture supernatants was measured,and the cell cycle distribution of lymphocyte was analyzed.ResultsWhen compared to the control group,the activity of Ca2+/CaN-NFATc [ (81.21 4-14.39) vs (63.66 ±9.02) ] was increased and the protein expression of CyclinE [ (0.9327 ±0.0370) vs (0.8374 ±0.0637) ] was higher in Lymphocyte of the asthma group ( P＜0.05,P ＜0.01,respectively).The percentage of lymphocyte in the S phase [ (7.8600±2.8241) vs (4.0270 ± 1.8650) ] and S + G2/M phase [ ( 10.6700±3.3850) vs (5.8740 ± 1.4389) ] was higher;however,the percentage of G0/G1 phase [ (89.3300 ± 3.3850) vs (94.1260± 1.4389 )] was lower in asthma group ( all P ＜ 0.01 ).The level of IL-4 [ ( 1.55 ± 0.19) pg/ml vs (0.99 ± 0.12 ) pg/ml ] and the IL-4/ IL-2 ratio [ (0.81 ±0.12) vs (0.49 ±0.49) ] in culture supernatants of the asthma group were higher than those of the control group ( all P ＜0.01 ).While the activity of Ca2+/CaN-NFATc (47.19 ±7.16)was decreased and the protein expression of Cyclin E (0.6840 ± 0.0485 ) was reduced in lymphocyte in CsA group(all P ＜0.01 ),the percentage of lymphocyte in the S phase (4.8600 ± 1.9595) and S + G2/M phase (7.9900 ± 1.9405) was decreased and the percentage of G0/G1 phase (92.2100 ± 1.9267) was increased ( all P ＜ 0.05 ),and the level of IL-4 (0.47 ± 0.09 ) pg/ml and the ratio of IL-4/ IL-2 (0.78 ±0.20) was lower in culture supernatants in the CsA group than that of the asthma group( all P ＜ 0.01 ).There was a positive correlation between the protein expression of dephosphorylated NFATc and the protein expression of CyclinE in Lymphocyte,so did between the protein expression of NFATc and the level of IL-4 in culture supernatants( r =0.711,P ＜0.01 and r =0.749,P ＜0.01.respectively).Conclusions The activity of Ca2+/CaN-NFATc was increased in lymphocyte of the asthmatic rats.Its increasing might result in the imbalance of Th1/Th2 by promoting the expression of IL-4 and might lead to the proliferation of lymphocyte by promoting the Cyclin E expression.

AIM: To investigate the effect of erythromycin on the level of transforming growth factor-?1(TGF-?1) and ?-glutaglutamylcysteine synthetase(?-GCS) in smoking rats,and to explore the antioxidate therapeutic role of erythromycin in chronic obstructive pulmonary disease.METHODS: Wistar rats were exposed to cigarettes smoking to establish the model.After passive smoking for 4 weeks,erythromycin intragastric intervention was administered continuously for 8 weeks.The expiratory airway resistance and lung compliance were assessed and the expression levels of TGF-?1 and ?-GCS proteins(and the mRNA) in airway endothelial cells and alveolar macrophages were observed respectively by immunohistochemical,immunocytochemical and(in situ) hybridization.RESULTS: The expiratory airway resistance was increased and the lung compliance was degraded significantly in smoking group and erythromycin group,compared to control group.In erythromycin group,the airway resistance was lower and the lung compliance was higher than that in smoking group(P

0.05] between group L and other two groups.Conclusion PCEA with 0.125% levobupivacaine containing fentanyl 2 ?g?ml-1 provides adequate pain relief after cesarean section with recovery of motor function similar to that with 0.2% ropivacaine and 0.125% racemic bupivacaine.