1.Method for studying traditional Chinese compound prescription
Jianyang XU ; Liwu LIANG ; Dewen QIU ; Xiao HAN
Journal of Integrative Medicine 2005;3(5):347-50
Treatment with traditional Chinese medicinal composite is one of the most important characteristics of traditional Chinese medicine (TCM). Studying material base of TCM composite and its mechanism is a key to modernizing the industry of Chinese medicinal herbs. The research for TCM composite can be carried out from many different angles, including multiple components, multiple actions, multiple levels and multiple targets. Such a way to study TCM composite will be beneficial to improving the theory of TCM composite, guiding clinical administration and developing new products.
2.Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine.
Fang YAN ; Yujun ZHAO ; Yongting HU ; Jianyang QIU ; Wenxin LEI ; Wenhui JI ; Xuying LI ; Qian WU ; Xiumin SHI ; Zhong LI
Journal of Veterinary Science 2013;14(1):53-60
The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody titers of the chickens immunized with pVAX1-16S1/M/N were much higher than those of the monovalent groups (p < 0.01). A protective rate up to 90% was observed in the pVAX1-16S1/M/N group. The serum antibody titers in the prime-boost birds were significantly higher than those of the multivalent DNA vaccine group (p < 0.01) but not significantly different compared to the inactivated vaccine group at 49 days of age. Additionally, the prime-boost group also showed the highest level of IBV-specific cellular proliferation compared to the monovalent groups (p < 0.01) but no significant difference was found compared to the multivalent DNA vaccine group, and the prime-boost group completely protected from followed viral challenge.
Aging
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Animals
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Antibodies, Viral/blood
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Cell Proliferation
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Chickens
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Coronavirus Infections/prevention & control/*veterinary/virology
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Immunization, Secondary/veterinary
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Infectious bronchitis virus/*immunology
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Poultry Diseases/*prevention & control/virology
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T-Lymphocyte Subsets/cytology/physiology
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Vaccines, DNA/immunology
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Vaccines, Inactivated/immunology
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Viral Vaccines/*immunology