A new flow-injection chemiluminescence method for the determinstion of trace amounts of sulfide was developed. It is based on the catalysis of sulfide to chemilumincence reacion between luminol and hydrogen peroxid. The linear range extends from 2.0 × 10-9 to 8.0 × 10-7 g/mL and the detection limit is 5.6 × 10-10 g/mL.The relative standard deviation (RSD) is less than 5% for 1.0 × 10-7 g/mL sulfide solution in eleven repeated measurements. The method has the advantages of simplicity, rapidity, sensitivity and has also been applied to the determination of trace amounts of sulfide in water samples.