OBJECTIVE: To establish a RP - HPLC method for simultaneous determination of the concentrations of phe-nobarbital, phenytoin and carbamazepine in serum.METHODS: Phenobarbital, phenytoin and carbamazepine were extracted from serum with CH2Cl2 and analysed using C18 column with mobile phase consisting of methanol - water(57 : 43); the column temperature was 30℃ ; the detecting wavelength was 254nm and the flow rate was 0.8ml/ min.RESULTS: The calibrating curves of phanobarbital, phenytoin and carbamazepine were linear in the ranges of 2.5 - 40, 2.5-40 and 1.25-20?g/ ml respectively .The within- day and between- day RSDs were less than 10% (n = 5) .CONCLUSION : This method is simple and reliable and suitable for phenobarbital,phanytoin and carbamazepine monitoring.

Objective To analyze the efficacy and safety of losartan in the treatment of massive proteinuria in kidney transplant recipients.Methods All of the 82 patients were randomized in two groups:losartan group and control group(amlodipine group).Both of the groups were divided into two different subsets according to blood pressure control Twenty-four-hour proteinuria,serum creatinine,blood pressure and adverse effects were observed.Results Losartan and amlodipine had the similar effects on blood pressure control The 24-h proteinuria in losartan group at the end of the study was significantly lower than that at the baseline,and there was significant difference between the losartan blood pressure control subset and the losartan blood pressure un-control subseL The effective rate and significant effective rate in losartan group for massive proteinuria were higher than in control group.Conclusion T Losartan can be effectively and safely used for the treatment of massive proteinuria in renal transplant recipients independent of blood pressure.

Objective To investigate the changes of protein and energy intakes and the z-score of weight for age in appropriate for gestational age (AGA) and small for gestational age (SGA) preterm infants with gestational age less than 34 weeks. Methods The data from 314 hospitalized premature infants ( 268 cases of AGA and 46 cases of SGA) during January 2012 to December 2014 were retrospectively collected. The intakes of protein and energy and the changes of weight within 2 weeks after birth were compared. Results Compared with AGA group, the hospital stays, durations of parenteral and enteral nutrition and total enteral nutrition, and time to achieve full dose feeding were signiifcantly longer in SGA group (P?

As an effective anticancer drug, the clinical limitation of doxorubicin (Dox) is the time- and dose-dependent cardiotoxicity. Yes-associated protein 1 (YAP1) interacts with transcription factor TEA domain 1 (TEAD1) and plays an important role in cell proliferation and survival. However, the role of YAP1 in Dox-induced cardiomyopathy has not been reported. In this study, the expression of YAP1 was reduced in clinical human failing hearts with dilated cardiomyopathy and Dox-induced