Objective To explore the efficacy and safety of three types of progesterone dosage forms in treatment of functional uterine bleeding, and compare their cost-effectiveness. Methods 180 cases with functional uterine bleeding were radomly divided into A,B,C three groups,each had 60 cases. On the basis of oral estradiol valerate,A,B and C group were given progesterone capsules,progesterone suppository and progesterone vaginal gel, respectively. The efficacy and cost-effectiveness were observed and compared. Results The efficacy rate of A,B and C group were 71.67%,86.67%and 93.33%,respectively. The adverse reaction ratio of A,B and C group were 5.00%,3.33% and 6.67%,respectively. The cost-effectiveness ratios were 336.26,98.80 and 1501.60,respectively. The increment of cost-effectiveness of A group and C group were-1035.80 and 19751.57.Conclusion Progesterone suppository(B group)has best effect in treatment of functional uterine bleeding.

Objective To evaluate the effects of Lactobacillus helveticus fermented milk on blood pressure and heart rate was evaluated in 8w old spontaneously hypertensive rats(SHR).Method Spontaneously hypertensive male rats were randomized into 5 groups(n=8) receiving fermented milk and its whey,water,skim milk and yogurt.Every sample was used to SHR on consecutive three days,during which they received increasing doses by ig.The systolic blood pressure and heart rate were measured by ZH-HX-Z tail cuff with a programmed electro-sphygmomanometer.Results At 1.5 ml dose of Lactobacillus helveticus TUST005 fermented milk and the 2 ml dose of Lactobacillus helveticus TUST005 fermented milk whey,we observed the marked and long-lasting hypertensive effect for 4 and 6 h,respectively.The maximum decrease in mean arterial blood pressure(20.5 mmHg) was achieved.Feeding 1 ml whey of Lactobacillus helveticus TUST005 fermented milk elicited significant decrease in heart rate(maximum of 50 bpm).Conclusion Significant reductions in mean systolic blood pressure,and heart rate were observed following intra-gastric administration of Lactobacillus helveticus TUST005 fermented milk.

Aim To study preparation of lutein from the extraction Marigold flowers by biotransformation.Methods Screen bacterium for lutein esters biotransforming by studying on the biotransformation of lutein esters by Penicillium on tabulate revulsive culture medium.Porule of and Aspergillus niger Penicillium could transform lutein esters into lutein,but biotransformation of Penicillium was better than Aspergillus niger.Porule of Pleurotus ostreatus could not grow on revulsive culture medium,but Pleurotus ostreatus could grow on the culture medium and transformed lutein esters into lutein.Research of shaking flash fermentation of Penicillium for trans-forming lutein esters.When pH is 6.0,quantity of incubation is 2 bacteria plugs and fermentation time is 12～24 hours,the transformation is completed and the content of lutein is 91.6%(HPLC)。Result Prolonging the transformation time will improve the percent of lutein but the content of lutein decreased.Conclusion Biological transrormation can be used from calendula officinacis extract preparation.

Objective To analysis the β-globin gene mutation in β-thalassemia in the population of Wenzhou natives,and identify the major mutation in Wenzhou and further provide valuable information for genetic counseling,prenatal diagnosis and prevention programs in this region.Methods Patients with β-thalassemia were diagnosed and the genomics DNA were extracted from whole blood cells and amplified with PCR,sequenced and compared to the standard sequence.Some mutations were further identified by subcloned.Results 44 of 66 patients were diagnosed β-Thalassemia,9 mutations were found in the 44 sporadic patients with the sequence analysis,2 of which were known polymorphisms(exonl 59,IVS-2-665),3 belonged to the common mutations in Chinese(IVS-2-654,CD_(41/42)-TTCT and TATA box nt-28),2 were scarce abnormalities(CD_(47),CD_(66))and 2 novel variants(-24T→C,CD_(26A)→G,same sense mutation,unreported).Conclusion The mutations of β-globin gene in Han Chinese in Wenzhou are complex (9 mutations found in all),the rare and novel mutations are identified,which provide the valuable information for genetic counseling in Wenzhou.

Objective To investigate and understand the reference value range of hematological parameters for peripheral blood routine in preschool children from Shanghai.Methods The Sysmex XS-800i automated hematology analyzer and the original rea-gents were used to measure the hematological parameters in peripheral blood samples collected from 7 692 healthy preschool chil-dren aged 2-6 years in Shanghai,including white blood cells(WBC),red blood cells(RBC),platelet(PLT)count,hemoglobin(Hb), hematocrit(HCT),mean corpuscular volume(MCV),mean corpuscular hemoglobin(MCH)and mean corpuscular hemoglobin con-centration(MCHC);the various parameters in different age groups were collected and statistically processed to establish the refer-ence intervals for each parameter.Results In the blood routine among preschool children in Shanghai,there were statistically signif-icant differences in all parameters except WBC and PLT count between sexes among preschool children in Shanghai(P <0.05).The reference intervals of hematological parameters obtained in this investigation were obviously differed from those offered by the man-ufacturer.However,compared with those from the related reports,the difference existed in partial parameters,the upper limit of WBC count was highest compared with the results from some areas,while the reference value ranges of MCV,MCH and MCHC were higher than those of other area study results.Conclusion The independent blood routine medical reference value range for preschool children in Shanghai should be established and the influence of the factors of gender,instrument and reagents also should be taken into consideration in establishing the reference value range.

It is important to investigations of early course and pathophysiologic mechanism of cerebral hemorrhage for drawing therapy and prognosis.This paper would review the formation and enlargement of hematoma after bleeding.

ObjectiveTo study the change of cholinergic neurons of hippocampus of vascular dementia rats after transplantation of the neural stem cells(NSCs) in hippocampus.MethodsAfter the vascular dementia model was reproduced,the rats were randomly divided into transplantation,dementia and pseudo-operative groups.8 weeks after operation,immunofluorescence was used to observe the survivors and migration of neural stem cells.Immunochemistry staining was used to observe the number of ChAT positive neurons in hippocampus.ResultsThe number of the ChAT positive neurons in the CA1 subfield of the hippocampus were more significantly increased in transplantation group than that in dementia group. ConclusionChAT positive neurons can be found in hippocampus area of vascular dementia rats after transplanting NSCs into the hippocampus, which seem to be cholinergic neurons.

Objective To explore whether patients with bipolar disorder are deficit in their theory of Mind (ToM) and the factors influencing the ToM.Methods Three groups (27 with bipolar disorder,27 with schizophrenia,and 26 in health control) were tested.All subjects' ToM was assessed by Faux pas Questions and IQ by WAISR.The symptoms of groups of patients were measured by Positive and Negative Symptom Scale (PANSS).Results Three groups' scores of Faux pas Questions(ToM scores) displayed significance(One-Way ANOVA:F(2,77) =13.686,P =0.000) ; Post-hoc test (Games-Howell) showed that ToM scores (13.81 ± 5.66) of bipolar disorder group were lower than those of the control group (17.04 ± 3.79) and higher than the schizophrenia group (9.44 ±6.13).The difference was significant (P =0.048,0.024).Independent sample t test showed that ToM scores of gender in bipolar disorder group were not significant (male:13.20 ± 6.07,femal:14.58 ± 5.26,t =-0.623,P =0.539).There was no correlation between ToM scores of patients with bipolar disorder and their age,years of education,age of onset,duration of illness,scores of negative,positive and general psychiatric rating scale of PANSS,and IQ (＞ 70).Linear regression by stepwise regression was used on each specific symptom of the PANSS,and according to the outcome,related factor was selected as independent variables,the ToM scores as the dependent variable.The regression equation was ToM scores =21.79-1.53 G12-3.16 N6 ;R2adjust was 0.46.Conclusion Patients with bipolar disorder as a whole compared to the control have deficit in their ToM,and the deficit is less than that of patients with schizophrenia.G12 and N6 of the PANSS are the influencing factors,which explain 46％of the variation sources.

Objective To establish the pediatric reference intervals for Troponin Ⅰ and Creatine kinase-MB (CK-MB).Methods Healthy children (223 boys and 162 girls) aged from 7 months to 12 years old were studied.Blood specimens were collected and centrifuged,all serum samples were kept refrigerated below-70 ℃.Troponin Ⅰ and CK-MB were measured on the UniCel DxI 800 immunoassay system (Beckman Coulter) on the same day.Nonparametric statistics was used to estimate the 99th percentile reference intervals.Results The children were divided into three groups (group A 128 cases; group B 128 cases;group C 129 cases) according to age.The upper reference limits for CK-MB were dependent of age,and the group A (7 months-1 years) was shown to be 13.41 μg/L,the group B (2-3 years) was shown to be 9.35 μg/L,the group C (4-12 years) was shown to be 5.48 μg/L.The upper reference limit for Troponin Ⅰ was independent of age or gender and shown to be 0.01 μg/L among 385 children.Conclusions Pediatric reference intervals for Troponin Ⅰ and CK-MB have been defined in healthy children at a relevant age group (7 months-12 years).It is effective for clinical diagnosis and treatment of myocardial damage for children by determining reference intervals for Troponin Ⅰ and CK-MB.

Objective To investigate the effects of leonurus heterophyllus injection and oxytocin and their combination applica-tion in treating postpartum hemorrhage .Methods Sixty cases of cesarean section due to the medical factors or the social factors and 54 cases of vaginal delivery were randomly divided into 3 groups by the blind method ,i .e .the leonurus heterophyllus injection group ,the oxytocin group and the leonurus heterophyllus injection plus oxytocin group .Each group was treated by the specific mode .The amounts of intraoperative bleeding and at postpartum 48 h bleeding were recorded ,and the time of the third stage of la-bor was recorded .One way Anova was used to analyze the obtained data .Results The intraoperative bleeding amounts were (1 014 .75 ± 159 .10) mL in the leonurus heterophyllus injection group and the cesarean section group ,(433 .88 ± 75 .34) mL in the leonurus heterophyllus injection plus oxytocin group and (562 .30 ± 102 .00) mL in the oxytocin group ,the difference among the groups were statistically significant(F=67 .48 ,P<0 .01) .The post hoc LSD test showed that under P<0 .05 ,LSD=109 .58 ,indi-cating the significant differences between the two groups ;in the vaginal delivery group ,no statistical difference in the total bleeding amounts ,postpartum 2 h and 2-6 h bleeding amounts had no statistical differences among 3 groups ,but the other time periods of detection had difference ,in which ,compared with the oxytocin group ,postpartum 24 h bleeding amount in the leonurus heterophyl-lus injection group was relatively less ;in the cesarean section group ,the third stage of labor had no statistical difference among 3 kinds of treatment group .Conclusion Using leonurus heterophyllus injection after vaginal delivery can reach the similar effect as oxytocin .However ,leonurus heterophyllus injection is not recommended to be exclusively used in cesarean section .