Objective To investigate the effects of rhGH on rat model of postoperative fatigue syndrome (POFS) and to study its mechanism. Methods A total of 36 SD rats were randomly divided into 3 groups. The model group and rhGH group were estalished into the model of POFS by partial resection of the liver, and administrated with the same volume of physiological saline and rhGH, and control group was without any treatment. The behavioral changes and the disorder of nutrition intake after operation, stress reaction (pathological changes of mucous membrane in small intestine) and the liver albumin expression were observed. Results The rhGH could improve behavioral changes of rat model and increase the serum levels of the iron, total protein, albumin and globulin as the index of nutrition, and restore the injury of the mucous membrane resulted from the stress reaction and increase the expression of the liver albumin. Conclusion rhGH can shorten the time of POFS and mitigate POFS of rat model.

Objective To establish and assess a model of abdominal postoperative fatigue syndrome (POFS) in rats. Methods After 70% hepatectomy was performed, the following observations of the animals were made:general condition, rat tail suspension test,weight carrying swim fatigue test,serum levels of albumin,ferrition,and iron,pathologic assessment of injury of small intestinal mucosa and hepatic albumin gene expression .Results After 70% hepatectomy of the rats,their general candition was poor,the level of physical tolerance decreased,they showed a certain amount of depression,and marked changes were found in nutritional index,stress injury of small intestinal mucosa and hepatic albumin gene expression.Conclusions A 70% hepatectomy rat model has the basic characteristics of clinical abdominal POFS, and can be used as an experimental animal model for the study of abdominal POFS.

Periphral lymphocytes suspended in RPMI-1640 were incubated in plates of 96 wells for 96 hours with 50 ?g/ml of PHA, various concentrations of LDL-ch and0.6 ?M of mevinolin (experimental) or equal volume of its solvent-dimethyl sulfoxide (control). 6 hours before harvesting cells, 0.5?Ci of ~3H-TdR was added to each well, then cells were harvested and their cpm counted. The LDL receptor activity was expressed as mevinolin-mediated lymphocyte proliferation inhibition rate which can be calculated by the following equation:Percent Inhibition Rate=(1-cpm exp./cpm con.)100%.It was found that the lymphocyte proliferation inhibition rate at 5?g/ml of LDL-ch was less than 20% for normal and non-FH hyperlipidemic subjects, 25-55% for FH heterozygotes and over 60% for FH homozygotes. Thus, FH patients were distinguished from normal subjects and non-FH hyperlipidemic patients.

AIM:To investigate the effect of tanshinone IIA on the apoptosis of human umbilical vein endothe -lial cells (HUVECs) after high glucose treatment.METHODS:The cell viability was determined by MTT assay.The cell apoptotic rate was examined by flow cytometry with Annexin V-FITC/PI double staining .The expression of Bcl-2 and Bax, and the release of mitochondrial cytochrome C ( Cyt C) were analyzed by Western blotting .RESULTS: Tanshinone IIA significantly inhibited high glucose-induced decrease in cell viability and increased the cell apoptosis .Additionally, after tanshinone IIA treatment , Bax expression and the release of mitochondrial Cyt C were significantly inhibited , while Bcl-2 expression was increased .CONCLUSION: Tanshinone IIA prevents high glucose-induced endothelial cell apoptosis via mitochondria-dependent pathway .

ObjectivesTo explore the clinical manifestations and treatment ofMycoplasma pneumoniae pneumonia (MPP) combined with venous thrombosis.MethodsThe clinical manifestations and treatment of MPP combined with venous thrombosis were retrospectively analyzed.ResultsTwo male patients were 5 years 2 months and 3 years 6 months old respec-tively. After treatment with erythromycin, corticosteroids, immunoglobulin, bronchoalveolar lavage and thrombolytic, both cases were cured. Conclusions It is necessary to pay attention to the occurrence of thrombosis in MPP. Early diagnosis and treatment of thrombosis is very important for the prognosis. The prevention of thrombosis needs to be further studied.

Objective To investigate the value of prevention of cerebral ischemia with multi-modality neuroelectrophysiological monitoring in intracranial aneurysm embolization. Methods The clinical data of 44 patients with intracranial aneurysm treated with endovascular embolization from May 2013 to June 2014 were analyzed retrospectively. The patients were divided into two groups according to whether they used intraoperative neuroelectrophysiological monitoring. There were 21 patients in a monitoring group and 21 in a non-monitoring group. According to the site of the aneurysms,somatosensory evoked potential ( SEP),motion evoked potential( MEP),scalp electroencephalogram,and brainstem auditory evoked potential (BAEP)were monitored,and at one day after procedure,their newly developed neurological deficits and the follow up observation after 3 months in both groups were compared and analyzed. Results The intraoperative SEP,MEP,and scalp EEG changes in the monitoring group were observed in 9,3 and 4 patients,respectively. Timely preventive measures were taken for 9 of the patients with cerebral ischemia revealed by neuroelectro-physiological monitoring. At the first day after procedure,the incidence of patients with new neurological deficit was 17. 4%(4/23)in the monitoring group,and that was 47. 6%(10/21)in the non-monitoring group. There was significant difference between the 2 groups(χ2 =4. 623;P<0. 05). There was significant difference in the good prognosis rates(87. 0%[20/23]vs. 57. 1%[12/21])after 3-month follow-up (χ2 =4. 919;P<0. 05)between the 2 groups. There were no deaths in both groups. Conclusion Multimode in combination with neuroelectrophysiological monitoring may decrease ischemic complications in aneurysm interventional treatment and improve the safety of procedure.

Objective To investigate and understand the reference value range of hematological parameters for peripheral blood routine in preschool children from Shanghai.Methods The Sysmex XS-800i automated hematology analyzer and the original rea-gents were used to measure the hematological parameters in peripheral blood samples collected from 7 692 healthy preschool chil-dren aged 2-6 years in Shanghai,including white blood cells(WBC),red blood cells(RBC),platelet(PLT)count,hemoglobin(Hb), hematocrit(HCT),mean corpuscular volume(MCV),mean corpuscular hemoglobin(MCH)and mean corpuscular hemoglobin con-centration(MCHC);the various parameters in different age groups were collected and statistically processed to establish the refer-ence intervals for each parameter.Results In the blood routine among preschool children in Shanghai,there were statistically signif-icant differences in all parameters except WBC and PLT count between sexes among preschool children in Shanghai(P <0.05).The reference intervals of hematological parameters obtained in this investigation were obviously differed from those offered by the man-ufacturer.However,compared with those from the related reports,the difference existed in partial parameters,the upper limit of WBC count was highest compared with the results from some areas,while the reference value ranges of MCV,MCH and MCHC were higher than those of other area study results.Conclusion The independent blood routine medical reference value range for preschool children in Shanghai should be established and the influence of the factors of gender,instrument and reagents also should be taken into consideration in establishing the reference value range.

In this paper, we studied the kinetics of nitochondrial lactate dehydrogenase (LDH) and solubilized mitochondrial LDH in the rat liver. The apparent Km values of mitochondrial LDH and solubilized mitochondrial LDH for substrate pyruvate were 50.0 ?mol/L and 33.8 ?mol/L, and those for NADH were 35.3?mol/L and 21.4 ?mol/L, respectively. The apparent Km values of mitochondrial LDH were greater than those of solubilized nitochondrial LDH. The mitochondrial LDH in the rat liver was mainly LDH-5, which could be solubilized by 0.15 mol/L NaQ solution.

In this paper, the effect of asolectin liposome on the activity of rabbit skeletal muscle lactate dehydrogenase (LDH) is reported. The results suggested that asolectin liposome could inhibit LDH activity, KC1 could restore the enzyme activity, and NAD+ and NADH could protect the enzyme from being inhibited by asolectin liposome.

Objective To explore the possibility for preventing the underwater blast injuries. Methods Fifty rabbits were divided into two groups: protection and control groups. The animals were placed at the rang of 5.0m to 17.5m far from the explosion point. 0.2kg of TNT Explosives was placed 3 meters under water. Physical parameters of blast wave were measured using pressure transducers. At 6 hours after injury, the mortality rate and morphological alternations were observed. Results The safe devices were shown to be effective against underwater blast injuries. Most of the animals had no or mild pulmonary and intestinal injuries. The whole injury severity was reduced by 2 to 3 degrees with our own made device. Conclusion Our safe device could be used to protect against underwater blast injueirs.