The ovary development in autogenous and anautogenous strains of Aedes togoi was stud led.Under the laboratory conditions, autogenous and anautogenous strains were selected from the wild caught Aedes togoi, and different stages of ovary development were observed.The experiment demonstrated that the biological characteristics of the ovary development in these two strains were evidently different, such as the size and number of matured follicles, the continuity and the pace of developing pro:esses.The main cause for the differences is the time and amount of vitellogenin deposited in oocytes.

This report deals with the comparative study on the vitellogenesis in autogenous and anautogenous strains of Aedes togoi. Histochemical and immunohistochemical methods were used. It was found that rule of vitellogenesis is characteristic in different strains within the same species. Synthesis of vitellogenin in autogenous strain begins as soon as post-emergence and the process goes on continuously, up to the oocytes to take in vitellin for maturation. Vitellogenin synthesis in anautogenous strain starts after blood meal, and there is an apparent testing stage before blood feeding. The capacity of vitellogenin synthesis is also different between two strains.

Substantial evidence indicates that comprehensive, long-term cardiac rehabilitation, involving medical evaluation, prescribed exercise, cardiac risk factor modification, education, counseling, and behavioral interventions, provides protection in primary and secondary coronary heart disease prevention. However, cardiac rehabilitation programs are greatly underused in patients with coronary heart disease. The benefits of exercise-based cardiac rehabilitation on the risk factors of coronary heart disease including smoking, obesity, dyslipidemia, hypertension and dysglycemia as well as overall morbidity and mortality, and quantity and quality of life were reviewed. And the related con-cepts, history, core components, current status and progress of cardiac rehabilitation were also discussed.

Objective To explore the impact of insulin secretory function of adult islet cells from barium-alginate microencapsulation in vitro. Methods After weighting the pancreas,human pancreatic islets were isolated with type V collagenase and purified by Ficoll's discontinuous density gradient centrifugation.The islet cell yield and purity were evaluated with microscope by DTZ staining.Human islets were coated by barium-alginate microencapsulation,and the viability was assessed by insulin release assay in vitro.Results After isolation,the average number of islet was about 3600 ±447 per gram pancreas.It was about 2140±207 after purification with more than 70％ purity.At day 2,4 and 6 after islet cell culture in vitro,basal insulin concentrations from the culture medium was measured,and the mean insulin concentration (mU/L) in media of the microencapsulated islet group at 2nd,4th and 6th day were 3.302±1.63、3.504±1.10 and 2.921±1.13 respectively,and those non-microencapsulated islet group were 3.814 ± 1.49、4.175 ±1.60、3.617± 1.34.There were no significant differences between these two groups (P＞0.05).Conclusions The bioartificial pancreas has effective insulin secretory function in vitro without being affected by barium-alginate microencapsulation.

Objective To investigate the methods and feasibility of islets isolation and purification,and to get more islets with high purity and good function for clinical transplantation.Methods After being weighted,human pancreatic islets were isolated with type Ⅴ collagenase,and purified by Ficoll's discontinuous density gradient centrifugation.The yield and purity of islets were evaluated by dithizone(DTZ) staining under microscope,and the viability was assessed by insulin release assay in vitro.Results The average number of islets was about 3 600 ± 447 per gram pancreas after isolation and it was about 2140 ±207 after purification with more than 70％ purity.2,4,6 days after islet cell culture,the basal insulin concentration of the culture medium was measured,and it was 3.302 ± 1.63,3.504 ±1.10,and 2.921 ±1.13 (mIU/L/100 islets) respectively.Conclusion Collagenase digest and Ficoll's discontinuous density gradient centrifugation are effective methods for isolation and purification of human pancreatic islets.

In this paper, we studied the kinetics of nitochondrial lactate dehydrogenase (LDH) and solubilized mitochondrial LDH in the rat liver. The apparent Km values of mitochondrial LDH and solubilized mitochondrial LDH for substrate pyruvate were 50.0 ?mol/L and 33.8 ?mol/L, and those for NADH were 35.3?mol/L and 21.4 ?mol/L, respectively. The apparent Km values of mitochondrial LDH were greater than those of solubilized nitochondrial LDH. The mitochondrial LDH in the rat liver was mainly LDH-5, which could be solubilized by 0.15 mol/L NaQ solution.

In this paper, the effect of asolectin liposome on the activity of rabbit skeletal muscle lactate dehydrogenase (LDH) is reported. The results suggested that asolectin liposome could inhibit LDH activity, KC1 could restore the enzyme activity, and NAD+ and NADH could protect the enzyme from being inhibited by asolectin liposome.

Objective: To construct an eukaryotic expression plasmid containing gene coding for the hemagglutinin neuraminidase(HN) of Newcastle disease virus (NDV), and to study its mechanism and value in antitumor therapy. Methods: The HN cDNA was abtained from NDV with RT PCR and an eukaryotic expression vector of HN gene ( pcDNA3 HN ) was constructed. The antitumor effect was evaluated after injecting pcDNA3 HN into mice bearing B16 melanoma. Results: The HN cDNA of NDV was successfully cloned and pcDNA3 HN had a good expression in COS 7 cells. Animal experiments suggested that the pcDNA3 HN could significantly increase CTL and NK activity of tumor bearing mice. Conclusion:The eukaryotic expression plasmid containing the gene coding for the (HN) has the function of increasing CTL and NK activity of tumor bearing mice.

Objective To study the relationship of gastric carcinoma with serum trace elements and helicobacter pylori(Hp).Methods The contents of 6 serum trace elements and helicobacter pylori were determined in gastric carcinoma,chronic gastritis patients and normal control.Results The serum levels of Mg,Ca,Zn and Cu in the patients with gastric carcinoma were significantly lower than those in normal control Mg (0.71?0.15)mmol/L vs (0.97?0.26)mmol/L,Ca (1.68?0.28)mmol/L vs (2.03?0.31)mmol/L,Cu (12.27?5.91)mmol/L vs (16.57?3.55)mmol/L,Zn (16.18?4.77)mmol/L vs (21.78?7.37)mmol/L.The infection rates of Hp in the patients with gastric carcinoma and normal control were 71.4% vs 27.3%(?2=6.201,P=0.013),there was significant diversity.Conclusion Obvious changes of serum Mg,Ca,Cu,Zn and infection Hp may be related to carcinogenesis of gastric carcinoma.

Objective To explore the overexpression of the 14-3-3 sigma (14-3-3σ) gene effects proliferation in pancreatic cancer cells.Methods The 14-3-3σ protein level in 46 pancreatic cancers and 9 normal pancreases specimens were analyzed by immunohistochemistry.The mRNA and protein expression of 14-3-3σ in pancreatic carcinoma cell lines (BxPC3,CAPAN-1,PANC-1,AsPC-1,SW1990,MiaPaCa-2 and CFPAC-1) were detected by RT-qPCR and Western blot respectively.A recombinant plasmid of pEGFP-14-3-3σ was constructed and transfected into the pancreatic cancer cell PANC-1 by liposome,and the expression of 14-3-3σ was detected by Western blot and real time fluorescence quantitative PCR.Cell proliferation activity was determined by MTS assay.Results The 14-3-3σ protein level was higher in pancreatic cancer tissue than in normal pancreatic tissue.mRNA and protein expression of 14-3-3σ was highest in BxPC3,high in AsPC-1,SW1990 and CFPAC-1,low in PANC-1 and Capan-1,and lowest in MiaPaCa-2.The successfully constructed pEGFP-14-3-3σ was confirmed by RT-qPCR and Western blot.MTS assay showed cell proliferation activity was significantly enhanced by overexpression of the 14-3-3σ gene compared to negative and blank control cells.Conclusion The expression of 14-3-3σ was higher in pancreatic cancer compared with normal pancre atic tissue,and the 14-3-3σ gene enhanced the cell proliferation activity of PANC-1.Therefore,14-3-3σ may play an important role in pancreatic cancer development.