Objective This research was designed to reveal the influence of the new rural cooperative medical system (NRCMS) on anticoagulant therapy of warfarin in atrial fibrillation (AF) patients in Gaochun district,Nanjing city.The reasons of not attending NRCMS and the relationship between NRCMS and the reasons of not correctly anticoagulant therapy were also investigated.Methods We collected outpatients and inpatients in Gaochun district in our hospital who suffered with AF and did not attend any medical insurance except NRCMS.Patients' data of whether enrolled in NRCMS,whether anticoagulated with warfarin,whether measure international normalized ratio and then confine it in therapeutic extent and fundamental data of gender,age etc.were collected by questionnaire.Then,the data were analyzed.Results A total of 1 094 patients were included in our research of which 896(81.9％) patients were enrolled in NRCMS.198 subjects did not attend NRCMS.The most common reason was not getting the message of NRCMS,which covered 104 patients (52.5％).Among the patients without counter indications of warfarin,801 patients did not be anticoagulated,of which 637 were covered by NRCMS and 164 were not insured by NRCMS,which accounted for 71.1％ and 82.8％ of their total respectively.The difference was statistically significant (x2 =11.387,P =0.001).Similar to the nonNRCMS,the most common reason of not accepted anticoagulation in patients covered by NRCMS was taking substitutions,which was hold by 42.9％ patients.All the differences of proportions of patients had recorded reasons with and without NRCMS had no statistical significance except for the proportion of patients who hold the reason of not knowing the necessity of anticoagulation,having difficulty in daily life,and compound reasons.Among the anticoagulated patients,202 cases did not restrict INR in therapeutic range,of which 170 cases were covered by NRCMS and 32 cases were not insured by NRCMS,which accounted for 65.6％ and 94.1％ of their total respectively.The difference was statistically significant (x2 =11.385,P =0.001).The most common reason of not restricting INR in patients covered by NRCMS was did not know the necessity of restricting INR,which was hold by 40.6％ patients.The proportions of patients who did not take anticoagulation with the reason of having difficulty in daily life had statistical difference,while the difference of other reasons did not meet statistical significance.Conclusion There were many patients did not attended NRCMS.NRCMS can improve the proportion of anticoagnlation therapy with or without correct ways.However,there were many patients did not treated with anticoagulation and there were many patients did not restrict INR in therapeutic rainge.Propaganda,health education and directions of health information are needed.

OBJECTIVE:To observe the therapeutic effect of compound glycyrrhizin on acute biliary tract infection com?bined with abnormal liver function.METHODS:The patients were randomly divided into trial group(141cases)and control group(120cases).The control group received ATP,CoA and inosine,and the trial group received compound glycyrrhizin60～120ml/d in addition to the treatment for control group.RESULTS:In trial group,113cases were remarkedly effective and28cases effective with a total effective rate of100%,and in control group,79cases were remarkedly effective and29cases effective with a total effective rate of90%.CONCLUSION:Compound glycyrrhizin has the anti-inflammatory effect and can protect the cell membrane of liver cells,shorten the hospital stay duration and decrease the occurrence of postoperative complications.

Objective To understand the effect of insulin-like growth factorⅠ (IGFⅠ) on the adaptation of the small intestine in growth hormone (GH)-treated rats. Method 20 SD rats were randomized into GH group and STD group. Parenterally fed, short bowel rat models were established. The morphological changes of the intestinal mucosal epithelia were examined, serum GH, IGFⅠ concentrations were determined by RIA method. The local intestinal IGFⅠ mRNA was evaluated by Northern blot method. Result At the end of the study, mucosal thickness, villous height, crypt depth in GH group (471?16, 299?17, 161?20 ?m respectively) increased significantly compared to that in STD group (374?13, 212?19, 96?9 ?m respectively, all P

AIM To investigate the significance of CGRP and ET in the coronary syndrome and the significance of their changes in the incidence of the acute coronary syndrome.Methods Group with UA group ( n =30), AMI group ( n =28), control group ( n =25) were established.In control group blood sample take on an empty stomach in the morning ,while group with AMI on hospitalization and after one week, group with UA at angina pectoris attack and after remission. The concentration of plasm CGRP and ET were measured .Results The plasm CGRP concentration in group with AMI was higher than that in group with UA and that in control group ,the plasm ET elevated markedly, while the rate of CGRP/ET decreased; Compared with that in the remission period of the acute coronary syndrome ,The plasm CGRP elevated, the plasm ET elevated markedly, while the rate of CGRP/ET decreased.Among defferent acute coronary syndrome ,the plasm CRGP and ET concentrantion was highest in AMI group,higher in group with accelerated UA, high in group with initial attack UA;the rate of CGRP/ET was highest in group with initial UA,higher in group with accelerated UA,high in group with AMI.Conclusions The changes of plasm CGRP and ET concentration are well correlated with the extent of coronary lesion, the level of plasm CGRP and ET and the imbanlance of thier receptor play an important role in the pathogenesis and development of acute coronary syndrome. [

Objective To investigate thermal degradation kinetic characteristics of Cefmenoxime Hydrochloride in infusion solutions, and predict its thermal stability. Methods The HPLC was applied to determine the contents of Cefmenoxime Hydrochloride. Classical isothermal kinetic method and multivariate linear model were used to predict the expiration date of the injection. Results It was found that the thermal degradation kinetics of Cefmenoxime Hydrochloride in two infusion solutions corresponded with the first-order kinetics. The expiration dates of Cefmenoxime Hydrochloride in 0. 9%sodium chloride injection calculated by two different methods were 2. 20 days and 1. 52 days,and in 5% glucose injection were 2. 09 days and 1. 53 days,respectively. Conclusion The thermal stability of Cefmenoxime Hydrochloride in infusion solutions is poor and its expiration dates are the same calculated by two different methods.

Objective To explore the short- term efficacy of argon- helium knife cryotherapy followed by chemotherapy in treating advanced non- small cell lung cancer (NSCLC) and to investigate its effect on the long- term survival. Methods During the period from March 2005 to March 2008, a total of 61 patients withⅢb or Ⅳ stage NSCLC received argon- helium knife cryotherapy followed by chemotherapy (study group), and other 52 patients with Ⅲb or Ⅳ stage NSCLC were treated with chemotherapy only (control group). The clinical data were retrospectively analyzed. The pain was evaluated by numeric rating scale (NRS) and the quality of life (QOL) was assessed by functional assessment of cancer therapy- general (FACT- G) scale. The clinical effect was evaluated according to RECIST criteria for solid tumor, and the patient’s survival time was recorded. Results (1) Twenty- six patients had local pain before the treatment, and the pain was relieved in different degrees after cryotherapy. The QOL, including all respects of FACT - G, was significantly improved after cryotherapy in all 61 patients. (2) The remission rate of the study group and the control group was 34.4% and 15.4% respectively, the difference between the two groups was statistically significant (P <0.05). (3) The median survival time of the study group and the control group was 12.9 months and 9.5 months respectively, and the one- year survival rate of the study group and the control group was 53.6% and 35.4% respectively. The differences between the two groups were statistically significant (P < 0.01). Conclusion Argon- helium knife cryotherapy is a safe and effective local treatment for advanced NSCLC, which can quickly reduce the tumor load, relieve the pain and improve patient’s quality of life. Cryotherapy with subsequent chemotherapy is superior to simple chemotherapy in improving the patient’s survival rate.

Object To observe the effect of Guizhi Fuling Capsule (GFC) on prostatic hyperplasia in rats induced by testosterone propionate. Methods Seven days after the male rats were castrated, testosterone propionate (3 mg/kg) was sc injected in rats. Meanwhile, the rats were administered GFC (2.16, 1.08, 0.54 g/kg) once a day, and the treatment continued for 30 d. An hour after the final administration, the rats were put to death, and the weight and volume of prostates were measured. The construction of prostate cells was also observed under light microscope. Results In the GFC group, the weight and volume of prostate was significantly decreased compared with that in the model group (P

Objective In order to study the effect of ? 1,4 GalT Ⅰ on proliferation of Schwann cells, The changes in cell proliferation were checked after Schwann cells transfected with sense or antisense ? 1,4 GalT Ⅰ plasmids. Methods Methods of counting numbers of proliferating cell and measuring [ 3H] thymidine incorporation by liquid scintillation were used to study the proliferation of purified Schwann cells which transfected with ? 1,4 GalT Ⅰ plasmids. Cell cycles of those transfected cells were determined by fluorescence activated cell sorting. Results After Schwann cells transfected with sense ? 1,4 GalT Ⅰ plasmids, the proliferation of those cells was restrained, and the number at G 1/G 0 phase of those cells increased while the number at S phase decreased. Schwann cells transfected with antisense ? 1,4 GalT Ⅰ plasmid showed opposite changes.Conclusion ? 1,4 GalT Ⅰ may play an important role in regulating the proliferation of Schwann cells in peripheral nerve.\;[

BACKGROUND:Degradable polymer materials initiate the degradation process immediately after implantation. How to regulate the degradation of these materials is rarely reported at present. OBJECTIVE:To study the effect of ultrasonic wave on control ing the degradation of polymer materials. METHODS:The sample is made ofε-caprolactone/L-lactide copolymer, and its core was coated with low density polyethylene on the surface with the fol owing four different methods. (1) The core surface was firstly covered with CaCl 2 powder, and then coated with polyethylene. (2) The core was firstly coated with polyethylene and coarsened for 3 hours. (3) The core surface was firstly covered with CaCl 2 powder, and then coated with polyethylene, and coarsened for 3 hours. (4) The core was directly coated with polyethylene. The four kinds of specimens obtained were embedded in pork for ultrasonic bombardment experiment in vitro. RESULTS AND CONCLUSION:In the specimens prepared with methods 1 and 4, the lyophobic layer could protect core materials before ultrasonic treatment, and no absorption peak was found at 631 nm. After ultrasonic treatment, the lyophobic layer was destroyed, toluidine blue dye was released, leading to change the color of immersion solution and increase the absorption peak at 631 nm. In the specimens prepared with methods 2 and 3,the lyophobic layer cannot exhibit the protection effects, the absorption peak was found at 631 nm. Under electron microscope, the appearance of the specimens in four groups was changed obviously. It is feasible to control the starting of the degradation by coating the degradable copolymer with LDPE and using ultrasonic as a trigger.

Objective To investigate the effect of early inflamation on the efficacy of gene(LacZ gene)(transfer) to vein graft by adenovirus vector.Methods The liquid containing adenovirus vector was infused through external carotid vein and the segment of carotid vein was resected.The veins were inflated naturally.After 30min incubation,the veins were transplanted into carotid artery in experimental group,and the(circulation) was restored in control group.The veins with transferred gene were collected on day 3,day 14 and day 21.The mark gene expression in the veins was studied with X-Gal stain and activities of ?-galactase were measured.ICAM-1 and VCAM-1 expressions were observed by immunohistochemistry stain in transgenic veins.Results The activities of ?-galactase in mark gene expressed for 14 days was decreased and in 21 day nearly disappearred.VCAM-1 and ICAM-1 expression upregulated after day 3 in transplanted veins and there was leucocyte infiltration and shedding of endothelial cells with endothelial disruption.On the other hand,there was no expression of VCAM-1 and ICAM-1 in control group veins and integrity of endothelium was intact.The positive ECs expressed mark gene was obviously reduced in transplanted veins compared to control group.But the number of positive SMCs were not significently different between to two groups.(Conclusions) The inflammation of vein grafts in early stage of transplantation may be the cause of(endotheliocyte) damage and is related to rapid depression of gene expression.