Eight-year medical education is to train medical personnel with high research abi lity and innovative ability, while the experimental teaching, as an important part of physiology teaching, plays an important role in training students in observation, problem-solving skills and practical ability. This article mainly discusses our exploration and practice of teaching in physiology experiment for eight-year program medical students from teaching contents and organization of class teaching, in order to fully increase the studying enthusiasms and meet the scientific mind of these students.

Objective with the advantages of rapidity in detection protein, We selected the gender-specific amino acid sequence based on human SMCY and SMCX, cloned and expressed SMCY gender-specific fusion antigens. The rabbits were immunized with purified antigens to obtain the polyclonal antibodies. A new method was established for rapidly sex identification of forensic evidence samples by detecting SMCY antigens with the corresponding polyclonal antibodies. Methods We found three differential fragments by analyzing the sequence of human SMCY and SMCX. Then we cloned this three fragments and ligated as a new recombinant.This SMCY gender-specific fusion antigen gene was sub-cloned into pET-28a and expressed in Escherichia coli. The fusion antigen was purified by Ni-NTA column. The rabbits were immunized with purified antigen to produce the specific polyclonal antibodies.The reactivity of the polyclonal antibody was evaluated by ELISA and Western blotting. We developed a colloidal gold test strip for detecting the gender of human samples. Results We successfully selected gender-specific amino acid sequence, the SMCY gender-specific fusion antigen was expressed by prokaryotic expression and the polyclonal antibody was prepared by immunizing rabbit. The results of colloidal gold strip tests showed that there is a significant difference between male and female serums. Conclusion The results showed that the SMCY gender-specific fusion antigen could be recognized by the polyclonal antibody.The colloidal gold strip tests made by SMCY gender-specific fusion antigens and the corresponding polyclonal antibodies could be used for rapidly determining the gender of forensic evidence samples.

Objective:To compare the short-term clinical outcomes of robotic distal pancreatectomy(RDP) with laparoscopic distal pancreatectomy(LDP).Methods:PubMed, Embase, Cochrane library, Wanfang data, CNKI were searched systematically.Studies that compared short-term clinic outcomes between RDP and LDP groups were included. Observation indicators include: operation time, intraoperative blood transfusion rate, spleen preserving rate, spleen vessels preserving rate, conversion rate to open surgery, complication rate, severe complication rate, pancreatic fistula rate, severe pancreatic fistula rate, length of hospital stay, etc. The Meta-analysis was performed by using RevMan5.3.Results:Eleven non-randomized controlled trials with 791 patients meet the inclusion criteria.This Meta-analysis shows: compared with LDP group, RDP group was associated with higher spleen preserving rate ( OR=2.32, 95% CI: 1.07-5.04, P=0.03), higher splenic vessels preserving rate ( OR=3.07, 95% CI: 1.10-8.57, P=0.03), lower conversion rate to open surgery ( OR=0.58, 95% CI: 0.35-0.97, P=0.04), shorter hospital stay ( MD=-2.42, 95% CI: -4.30 --0.55, P=0.01), longer operative time ( MD=27.11, 95% CI: 9.06-45.16, P<0.01). There was no significant difference in overall complications, severe complications, pancreatic fistula, severe pancreatic fistula, and transfusion rate between the two groups. Conclusions:RDP showed a slight advantage in short-term outcomes, and it is worthy of applying in large medical center. Further studies on the long-term outcomes of these surgical techniques are required.

Animals ; Mice ; Motor Neurons/physiology* ; Spinal Cord