1.Detection of p53 and p21~(WAF1) in cholangiocarcinoma
Xin WANG ; Jianxi ZHANG ; Jie CHEN
Chinese Journal of Hepatobiliary Surgery 1998;0(06):-
Objective To clarify the clinical significance of p21 WAF1 and the relationship between it and p53. Methods Samples of human cholangiocarcinoma (HC) tissue and paired normal bile duct tissue adjacent to the tumor from 30 patients with HC were employed in this study. In situ hybridization (ISH) was used to detect the p53 and p21 mRNA expression. Immunohistochemistry (IHC) was applied to analyze p53 gene mutation and P21 protein expression. Results p21 WAF1 And p53 gene protein expression was detected in 36.7% (11/30) and 53.3% (16/30) of the carcinoma specimens, respectively, and none of the paired tissue by IHC. p53 Positivity was related to local lymph node metastasis. Comparing with non-lymph node metastatic group, p53 positivity in metastatic group was significantly higher. p53 Positivity of cholangiocarcinoma in clinical stage III was significantly higher than that in clinical stages I and II. The survival time was significantly shorter in patients with P53 protein expression than in those without. It was found that p53 expression was not associated with p21 WAF1 expression. Conclusions P53 Positivity may be correlated with tumor development but not tumor occurrence. The fact that p53 expression was not associated with p21 WAF1 expression indicates that p53-independent activation of p21 WAF1 may exist.
2.Effect of Yaotui Litongsan on Nerve-related Behavior of Autograft of Nucleus Pulposus to Cavum Epidurale in Rats
Linqing WANG ; Sisheng ZHANG ; Jianxi PAN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(09):-
Objective To observe effect of Yaotui Litongsan on nerve-related behavior of autograft of nucleus pulposus to cavum epidurale in rats.Methods Fifty normal Wistar rats were randomly divided into five geoups:the sham operated group (A),injury model group (B),Yaotongning treat group (C),high dose and low dose group (Yaotui Litongsan treat,D and E),10 rats in each group.Autogenous nucleus pulposus was removed from the coccygeal vertebral and placed into the back of L5~6 nerve roots of left cavum epidurale,making the non-compressive model with transplanted autogenous nucleus pulposus.The mechanical withdraw threshold and thermal withdraw latency were determined.Results The significant mechanical hyperalgesia was showed in the rats of B,C,D,E groups postoperatively,but it was lighter in C,D,E groups than in B group (P
3.Clinical Study of Shengfuling on Skin Defect Healing of Human Body
Linqing WANG ; Jianxi PAN ; Sisheng ZHANG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(09):-
Objective To study the therapeutic effect of Shengfuling on the healing of various skin defect of human body.Methods Sixty cases of various skin defects were randomly divided into the treated group and the control group,which were treated by Shengfuling and normal healing after debridement,respectively.The healing time of defect areas and skin injury degrees was observed.The post-healing skin quality(color,hardness,tension) of the two groups were compared.Results The treated group had better effect than the control group in skin healing velocity and post-healing skin quality(P
4.Low-power design of wireless ECG monitor
Shi ZHANG ; Jianwei DONG ; Junhui WANG ; Shanguo ZHAO ; Jianxi YU
Chinese Medical Equipment Journal 2004;0(07):-
This paper introduces the low-power design of a portable wireless ECG monitor which adopts MC35 GPRS module.At first,it presents the hardware and software system.Secondly,according to the principle of low-power design,it describes the low-power design methods from the aspects of hardware and software respectively.Finally,this paper discusses how to improve this power management system.In practice,this design efficiently reduces the power-consuming,and prolongs the working time of the monitor.
5.Clinical research of the effects of carbon dioxide pneumoperitoneum on cardiovascular function in geriatric cholelithiasis
Jianliang ZHANG ; Kesen XU ; Qinye FU ; Sanyuan HU ; Jianxi WANG
Chinese Journal of Current Advances in General Surgery 1999;0(04):-
Objective: To explore the effect of CO 2 pneumoperitoneum in laparoscopic surgery on the cardiovascular function of the geriatric cholelithiasis.Methods:Sixty aged patients undergone laparoscopic surgery were selected.During various stage of CO 2 insufflation,the heart rate(HR),blood pressure,oxygen saturation ,electrocardiogram(ECG),and myocardial enzymes were dected,and the clinical values of these changes were analyzed.Results: when CO 2 pneumoperitoneum established ,the HR and blood pressure were increased obviously .The incidence of arrhythmia was 36% and the incidence of changes of ST segment was 30%,10 minutes after pneumoperitoneum eliminated,these changes were restored. The myocardial enzymes and the oxygon saturation of blood had no distinct changes. Conclusion: The procedure of CO 2 pneumoperitoneum caused temporany changes of the HR ,SBP/DSP and ECG.The laparoscopic surgery is effective and safe for geriatric cholelithiasis.It suggests that the dynamic monitoring of electrocardiogram is necessary,especially for those with abnormal heart function.
6.The Experimental Research in the Effects of Ultrasonication on Degradation of PLGA Released DNA
Haibo FAN ; Hai ZHANG ; Ying LI ; Yuan ZHANG ; Jianxi TANG ; Junhui CHEN ; Benyi LI
Chinese Journal of Biochemical Pharmaceutics 2014;(1):35-37
Objective To identify the effective results of ultrasound in degradation of polymeric nanoparticles released DNA .Polymeric nanoparticles was made by dehydration of polyacetylglutamicacid (PLGA, polylactic-co-glycolic acid)solution. Method Green Fluorescent Protein (GFP) was enclosed by PLGA. Different kinds of ultrasound mode and different duct cycle and power ones were used to radiate PLGA solution for 90 s, 9 min, 20 min separately after the solution prepared for 2 hrs,then putted the solution on centrifugal machine at 13000 r/m. Using Choloroform to get rid of fat-soluble impurity,then applied nanodrop to survey the releasing rate of DNA. Finally the effect of cell expression were observed by fluorescent microscope. Results The amount of DNA released from PLGA in groups which were exposed to ultrasound were significantly different from the groups which were not exposed to ultrosound. The releasing amount of former groups had upper limitation. The releasing rate was increased with the increment of the irradiation time,frequency of ultrasound;The effect of the DNA releasing and PLGA degradation by continuous-wave irradiation was stronger than pulsed-wave ultrasound. Conclusion Ultrasound can promote the degradation of PLGA, and do help in DNA releasing and expression in vitro.
7.Infant hand and forearm severe crush injury: A case of comprehensive microsurgery treatment
Qiqiang DONG ; Songjian ZHANG ; Shuqiang XIE ; Baofeng WANG ; Lei ZHANG ; Hongxin WANG ; Chaofan YANG ; Jianxi HOU
Chinese Journal of Microsurgery 2021;44(2):225-227
A case of a 3-year-old child suffered with severe crush injury to the right forearm and right hand on June, 2017. A comprehensive treatment was conducted with limb salvage, free flap repair and the repair of nerve, vessel and tendon for functional reconstructions followed by rehabilitation therapies. The function and appearance of the injured limb and hand recovered well 3 years after surgery.
8.Ultrasound enhances PLGA nanoparticle-mediated gene delivery in vivo
Hai ZHANG ; Ying LI ; Jianxi TANG ; Jun YANG ; Junhui CHEN ; Ying WU
Chinese Journal of Ultrasonography 2011;20(6):533-536
Objective To investigate the feasibility and the efficacy of ultrasound in promoting PLGA nanoparticle-mediated gene transfection in vivo.Methods Prostate cancer cell line PC-3 was used to generate xenografts in nude mice for gene transfection experiment in vivo.GFP plasmid was encapsulated in PLGA-based nanoparticles.Nanoparticles were injected into tumors locally.Two hours later,xenografts were exposed to ultrasound.Xenograft tissues were harvested in different time points to assess the efficiency of gene expression with regard to different parameters of ultrasound. Results PLGA nanoparticle-encapsulated GFP plasmids were readily transfected to PC-3 cells in vivo.A large number of GFP expressing cells were observed after exposed to ultrasound with 1.0 MHz 50% duty factor continuous wave.In comparison,ultrasound exposure with 40% duty factor pulse wave in vivo had low efficacy in terms of GFP expression.No animal death was noticed due to ultrasound exposure.Conclusions Ultrasound exposure can enhance the release of plasmid DNA content delivered by PLGA nanoparticles in vivo,local exposure to ultrasound wave would be used in conjunction with PLGA nanoparticle-mediated targeted delivery to the tissue or organ of interest.
9.Repair of sheep metatarsus defects by using tissue-engineering technique.
Zhanghua, LI ; Yi, YANG ; Changyong, WANG ; Renyun, XIA ; Yufu, ZHANG ; Qiang, ZHAO ; Wen, LIAO ; Yonghong, WANG ; Jianxi, LU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(1):62-7
Tissue-engineering bone with porous ,betatricalcium phosphate (3-TCP) ceramic and autologous bone marrow mesenchymal stem cells (MSC) was constructed and the effect of this composite on healing of segmental bone defects was investigated. 10-15 ml bone marrow aspirates were harvested from the iliac crest of sheep, and enriched for MSC by density gradient centrifugation over a Percoll cushion (1. 073 g/ml). After cultured and proliferated, tissue-engineering bones were constructed with these,cellS seeded onto porous f-TCP, and then the constructs were implanted in 8 sheep left metatarsus defect (25 mm in length) as experimental group. Porous ,-TCP only were implanted to bridge same size and position defects in 8 sheep as control group, and 25 mm segmental bone defects of left metatarsus were left empty in 4 sheep as blank group. Sheep were sacrificed on the 6th, 12th, and 24th week postoperatively and the implants samples were examined by radiograph, histology, and biomechanical test. The 4 sheep in blank group were sacrificed on the 24th week postoperatively. The results showed that new bone tissues were observed either radiographic or histologically at the defects of experimental group as early as 6th week postoperatively, but not in control group, and osteoid tissue, woven bone and lamellar bone occurred earlier than in control group in which the bone defects were repaired in "creep substitution" way, because of the new bone formed in direct manner without progression through a cartilaginous intermediate. At the 24th week, radiographs and biomechanical test revealed an almost complete repair of the defect of experimental group, only partly in control group. The bone defects in blank group were non-healing at the 24th week. It was concluded that engineering bones constructed with porous -TCP and autologous MSC were capable of repairing segmental bone defects in sheep metatarsus beyond "creep substitution" way and making it healed earlier. Porous ,-TCP being constituted with autologous MSC may be a good option in healing critical segmental bone defects in clinical practice and provide insight for future clinical repair of segmental defect.
Bone Marrow Cells/cytology
;
Calcium Phosphates/*pharmacology
;
Cells, Cultured
;
Fractures, Bone/*therapy
;
Implants, Experimental
;
Mesenchymal Stem Cells/*cytology
;
Metatarsus/*injuries
;
Porosity
;
Sheep
;
Tissue Engineering
10.Retroviral vector-mediated HBsAg expression and its stability.
Zhi ZHOU ; Haihong ZHANG ; Jianxi LU ; Jilu YAO ; Lainxian DENG
Chinese Journal of Experimental and Clinical Virology 2002;16(3):236-238
OBJECTIVETo explore use of retroviral vector in gene therapy of hepatitis B.
METHODSThe recombinant vector Plxsn-HBs was constructed by inserting HBV S gene into pLXSN. The pseudovirus, which was produced from PA317 after transferring with pLXSN-HBs by electroporation, were frozen at different temperature. The activities of the pseudovirus to infect eukaryotic cells and express antigen were determined by comparing the numbers of G418-resistant clones and assaying HBsAg in the supernatant of the cells with ELISA after infection HepG2, NIH3T3 and 293 cells.
RESULTSIt was hard to find changes in HBsAg amount at different intervals and different temperatures. G418 resistant clones, however, were variable. When frozen at -20 degrees C, the numbers of clones were half less than that of the beginning after 6 months, few clones were formed after 12 months, and no clone was found after 24 months. When frozen at -40 degrees C, the numbers of clones were 121, 332 and 89 42, 137 and 43 for HepG2, NIH3T3 and 293 cell lines at 12 and 24 months, respectively. When frozen at -70 degrees C, the numbers of clones were 159 463 and 112 for HepG2, NIH3T3 and 293 cell lines at 24 months, respectively. There was no statistical difference compared to that of zero months.
CONCLUSIONSThe activity of the peseudovirus to infect eukaryotic cells and expressed antigen was not changed after 2 years frozen at -70 degrees C.
Cell Line ; Genetic Vectors ; Hepatitis B Surface Antigens ; biosynthesis ; genetics ; Retroviridae ; genetics ; Temperature ; Transfection