1.Separation and Preparation of 5 Phenylpropanoids from Cinnamomum Cassia by Semi-preparative HPLC and Their Immunosuppressive Activity Investigation
Junfen ZENG ; Jianwu LU ; Hucheng ZHU
China Pharmacist 2016;19(9):1625-1628
Objective:To simultaneously separate 5 phenylpropanoids from Cinnamomum cassia by semi-preparative HPLC, and explore their immunosuppressive activity. Methods:After extracted by ethanol, the ethyl acetate part of Cinnamomum cassia was isola-ted by semi-preparative HPLC. The separation was conducted on an Ultimate XB-C18 (250 mm × 10 mm, 5μm) semi-preparative chro-matographic column and the mobile phase was acetonitrile-water with gradient elution. The detection wavelength was 330 nm, and the flow rate was 2. 5 ml·min-1 . The sample volume was 0. 3 ml. MS and NMR spectroscopic analysis were used to determine the config-urations. A CCK-8 method was used to detect the immunosuppressive activity of phenylpropanoids. Results: Totally 5 phenylpro-panoids were separated from Cinnamomum cassia by the semi-preparative HPLC, and identified as erythro-guaiacylglycerol, (7R,8S)-syringoylglycerol, (7S,8S)-syringoylglycerol, 3-methoxyphenyl-acrylaldehyde and O-methoxy cinnamaldehyde. The inhibitory rates of T cells and B cells of the compound 3 was more than 20% at the concentration of 800μmol·L-1 . Conclusion:The method is conven-ient with good separation effect, which can simultaneously separate 5 phenylpropanoids from Cinnamomum cassia, and among them, the compound 3 shows immunosuppressive effect to some extent.
2.Construction of scientific integrity system in Chinese universities——Lesson from Australian、UK and American
Yong LI ; Yi WEI ; Lin WANG ; Jianwu ZHU ; Hong CHEN
Chinese Journal of Medical Science Research Management 2015;28(2):121-123,126
By analyzing scientific research misconduct and its causes,this paper revealed the conflicts between interests of researchers and management,and proposed measures and suggestions to construct scientific integrity system in Chinese institutions by drawing on experiences from Australia、UK and America.
3.Need analysis of information resource for appropriate health technologies
Qisheng GAO ; Jianwu LIU ; Senxiang LIN ; Wei ZHU ; Zhijun XIA ; Xin WEN ; Jianmin JIANG
Chinese Journal of Medical Science Research Management 2013;26(6):397-398,420
The need analysis of information resource for appropriate health technologies is important for the related information transmission channel and promotion of those technologies at primary level.This paper introduces the current status of the information resource construction of appropriate health technologies in China,and analyzes the needs and means of transmission in terms of users,holders and spreader,taking Zhejiang as an example.Methods of literature review,questionnaire survey,interview,and symposium were employed.
4.Research on the model of cultivation of young scientists in hospital by setting mentors
Lin WANG ; Ju LI ; Jianwu ZHU ; Yong LI ; Yong YANG ; Hong CHEN
Chinese Journal of Medical Science Research Management 2016;29(2):125-128
Tutor system has been widely applied in the cultivation of talents, such as undergraduates, graduate students and young scientist.Scientific research tutor system promotes the interaction between tutors and students, and improves the level of personnel training.In view of the problems of cultivation of the young scientists in hospital, we established a model of cultivation of young scientists by setting mentors.After a preliminary practice, more significant results have been achieved.
5.Effects of acupoint laser irradiation on gastric acid secretion and NO content in chronic atrophic gastritis
Xuehui SHAO ; Jianguo WANG ; Xiaoli ZHANG ; Jianwu GAO ; Xiaobo ZHU ; Yueping YANG
Chinese Journal of Physical Medicine and Rehabilitation 2016;38(7):492-496
Objective To study the effects of He-Ne laser irradiation on the pH value of gastric juice, the ultrastructure of gastric parietal cells and the content of nitric oxide ( NO ) in chronic atrophic gastritis ( CAG ) . Methods Sixty healthy male Wistar rats were randomly divided into a normal control group, a model group, a drug group, an acupuncture group and a laser acupoint group, each of 12. A CAG model was successfully intro-duced to all except those in the normal control group through the administration of sodium salicylate and alcohol, combined with fasting and water deprivation, running fatigue and other interventions. The rats of the drug, acupunc-ture and laser acupuncture groups were given medications, acupuncture at Zusanli acupoint and laser irradiation at Zusanli acupoint accordingly. After 14 days of treatment, all of the rats were executed and diluted gastric juice and gastric tissues were collected. NO content in serum was determined using a nitrate enzyme reduction method, the pH value of gastric juice was measured, and the ultrastructure of the gastric mucosa was observed using a transmis-sion electron microscope. Results The average pH of the gastric juice and the average serum NO content were both significantly higher in the model group than in the normal control group. In the model group the membrane structure of the parietal cells was absent or partly dissolved and the mitochondria were swollen. Drugs, acupuncture and laser irradiating at the Zusanli acupoint all corresponded with reduced pH and NO content, but the most signifi-cant improvements were observed in the laser acupuncture group. In the laser acupuncture group the membrane structure of parietal cells was complete, and the cytoplasmic organelles had nearly returned to normal. Conclusions Laser irradiation can promote recovery from CAG, increasing the secretion of gastric acid and de-creasing the serum level of NO. Both have a good curative effect for CAG. Laser irradiation deserves further clinical research and promotion.
6.Impact of age and sex on virologic responses of peginterferon alfα-2a and ribavirin treatment in chronic hepatitis C
Jianwu YU ; Lijie SUN ; Peng KANG ; Yonghua ZHAO ; Bingzhu YAN ; Pengfei ZHU
Chinese Journal of Internal Medicine 2011;50(12):1002-1007
ObjectiveTo investigate the impact of age and sex on virologic responses rates to peginterferon alfα-2a and ribavirin treatment in patients with chronic hepatitis C.MethodsThe medical records of 449 chronic hepatitis C patients,treated with peginterferon and ribavirin in Department of Infectious Diseases,the Second Affiliated Hospital,Harbin Medical University,were retrospectively analyzed.These patients were divided into three groups according to age:patients <40 years (n =131 ),patients 40-50 years ( n =131 ) and patients > 50 years ( n =187 ).The virologic response rates,the incidences of side events,and the rates of patients receiving ≥ 80% of planned peginterferon alfα-2a or ribavirin dose were compared between male and female patients in the three groups.The influential factors on sustained virologic response (SVR) of patients were studied by multivariate analysis.Results For genotype 1,in patients < 40 years group,the SVR rate of female was significantly higher than that of male (75.0%,30/40 vs 54.0%,27/50; P <0.05 ) ; in patients 40-50 years group,there was no significant difference in the SVR rate between male and female (51.0%,25/49 vs 53.7%,22/41 ; P > 0.05 ) ; in patients >50 years group,the SVR rate of female was significantly lower than that of male (31.1%,19/61 vs 50.7%,34/67; P <0.05).For genotype 2,there were no significant differences in virologic response rates between male and female in the three groups.The incidence of adverse events of patients aged < 40 years group,40-50 years group,> 50 years group,were 51.1% (67/131),51.1% (67/131),and 70.6% (132/187),respectively,and the incidence of adverse events of patients aged > 50 years was significantly higher than those of other groups ( P < 0.001 ).For genotype 1,in patients > 50 years group,the rate of patients receiving ≥80% of planned ribavirin dose of female was significantly lower than that of male (42.6%,26/61 vs 62.7%,42/67; P < 0.05).In multivariate analysis,the independent factors associated with SVR of patients aged > 50 years were sex ( P =0.013 ),genotypes ( P =0.002 ),cirrhosis ( P =0.004 ),≥ 80% of planned ribavirin dose ( P =0.008 ) and presence of rapid virologic response (RVR) ( P =0.001 ).ConclusionsFor genotype 1 patients,in patients < 40 years group the SVR rate of female is higher than that of male; in patients 40-50 years group,male and female share similar SVR rates;in patients > 50 years group the SVR rate of female is lower than that of male.Age and sex has no impact on virologic responses rates for genotype 2 patients.
7.Application of microarrays in screening the antibacterial peptide associated genes of Musca domestica.
Leishan LIU ; Xiaobao JIN ; Jiayong ZHU ; Ping XIAO ; Yuanxiang LI ; Jianwu GONG
Chinese Journal of Biotechnology 2008;24(7):1300-1305
To screen the candidate genes associated with Musca domestica antibacterial peptides using DNA microarray technique, the hybrid probes were designed from the conservative domains of the encoded area of the insect antibacterial peptide genes in GenBank with biology software Designer 2.0, and were synthesized by a chemical process, with the assistance of the automated Gen III Microarray Spotter, those oligo probes were printed on a special ready-made glass, and a cDNA microarray was constructed. The total RNA was extracted from the fat body of Musca domestic third-instar larve induced after 24 hours by Escherichia coli and Staphylococcus aureus, the strands of cDNA were labled with fluoresceine Cy3 using the method of reverse transcription PCR, after prehybridization, hybridization and washing procedure, the results of hybridization were scanned using computer system, and the data were analyzed using the software of MIDAS, fifteen valid hybridization signals were detected through two times of hybridization and scanning (the positive samples as a control were excluded). DNA microarray technique can be successfully applied screen the candidate genes associated with Musca domestica antibacterial peptides, and further provide significant evidence to discover its antibacterial peptide new genes.
Animals
;
Antimicrobial Cationic Peptides
;
genetics
;
Base Sequence
;
Gene Expression Profiling
;
Genes, Insect
;
Houseflies
;
genetics
;
growth & development
;
Larva
;
genetics
;
growth & development
;
Molecular Sequence Data
;
Oligonucleotide Array Sequence Analysis
;
methods
;
Oligonucleotide Probes
;
chemistry
8.Improving the quality of Young-Scholar-Science-Foundation project application by means of the mentorship scheme-Practice and experience
Lin WANG ; Yin WANG ; Jianwu ZHU
Chinese Journal of Medical Science Research Management 2019;32(1):52-55
Objective To improve the success rate of hospital Young Scholar Science Foundation project application,promote the rapid growth of young scientific talents;enhance the capacity of scientific research talents in hospitals Methods The status quo and existing problems of the application and funding of hospital youth science fund were analyzed;explore the construction of youth science fund mentor mechanism,measurements,such as setting up tutoring group,formulate tutoring plan,clarify mentoring details and follow-up implement supervision assessment were developed to strengthen the guidance of young scientific talents.Results Compared to last year,the number of acquisition of Young-Scholar-Science-Foundation projects has risen by 7,and the rate of acquisition in the 30 applications involved in the mentorship scheme reached up to 33.3%,and the difference between Mentorship-Scheme-Group and the other was statistically significant(P<0.05).Conclusions The implementation of the mentor system has significantly improved the quality of the application of the hospital's youth science fund.The next step is to continually improve the mentor system management and operation mechanism,strengthen the preliminary work support and project implementation guidance for young scientific talents,ensure the stable and well development of the application work of Young-Scholar-Science-Foundation projects.
9.Exploration and Reflection on Improving the Efficiency and Quality of Ethical Review in Multi-center Clinical Trial
Jingjing WANG ; Shizhi FAN ; Xiangli LIAO ; Hongyan DUAN ; Jianwu ZHU
China Pharmacy 2019;30(23):3196-3199
OBJECTIVE: To explore the ways to improve the efficiency and quality of ethical review in multi-center clinical trial. METHODS: After issuing the policy of Opinions on Deepening the Reform of Approval System and Encouraging the Innovation of Pharmaceutical Medical Devices and Measures for the Administration of Drug Registration (Revised Version), combined with the practice of our center, the methods to improve the efficiency and quality of ethical review, the possible problems of complete filling system and the way to improve the efficiency and quality of ethical review in multi-center clinical trial were analyzed in our center. RESULTS & CONCLUSIONS: Our center adopted a variety of review methods (conference review, rapid review, filing system, etc.), implemented hierarchical management and differential review process for research projects, and formulated defined rules of filling system operation and other ways to improve the efficiency and quality of review, doubled the review efficiency, increased the number of follow-up review and field visit. In the process of exploration, the ethics committee of our center believes that the complete implementation of the filing system will lead to problems such as the difficulty to evaluate the quality of the ethical review of the leader unit, different research qualifications and conditions of each center, etc. In order to improve the efficiency and quality of the ethical review of multi-center clinical research, the measures are can be adopted, such as improving the quality of the ethical review of the center, strengthening the communication and mutual recognition of the ethics committees of each center, and establishing the preliminary review system of the secretary of the ethics committee, so as to realize the balance of efficiency and quality.
10.Clinical features of severe or critical ill patients with COVID-19.
Weidang XIE ; Shijie ZHU ; Yanan LIU ; Yujia BAI ; Weijun FU ; Hui CHEN ; Zhongqing CHEN ; Jianwu ZHANG
Journal of Southern Medical University 2020;40(8):1112-1118
OBJECTIVE:
To analyze the clinical features of severe or critical ill adult patients with coronavirus disease (COVID-19).
METHODS:
The clinical data of 75 patients with severe or critical COVID-19 in Honghu People's Hospital from January to March in 2020 were collected.
RESULTS:
Of the 75 patients with COVID-19 pneumonia, 41 were male (54.67%) and 34 were female (45.33%) with a mean age of 67.53 ±12.37 years; 43 patients had severe and 32 had critical COVID-19, and 49.3% of the patients had underlying diseases. The main clinical manifestations included fever (78.67%) and coughing (70.67%). Compared with the severe patients, the critically ill patients had higher proportions of patients over 60 years old with elevated white blood cell count, increased prothrombin time, and higher levels of hsCRP, PCT, D-dimer, ALT, LDH, cTnI and NT-proBNP. Univariate logistic regression analysis showed that an age over 60 years, leukocytosis, hs-CRP elevation, prolonged prothrombin time, and increased levels of D-dimer, NT-proBNP and cTnI were associated with severe COVID-19. Multivariate logistic regression showed that an age over 60 years (OR=8.165, 95% : 1.483-45.576, =0.017), prolonged prothrombin time (OR=7.516, 95% : 2.568-21.998, =0.006) and elevated NT-proBNP (OR=6.194, 95% : 1.305-29.404, =0.022) were independent risk factors for critical type of COVID-19.
CONCLUSIONS
An age over 60 years, a prolonged prothrombin time and elevated NT-proBNP level are important clinical features of critically ill patients with COVID-19, and can be deemed as early warning signals for critical conditions of the disease.
Aged
;
Betacoronavirus
;
Coronavirus Infections
;
Critical Illness
;
Female
;
Humans
;
Male
;
Middle Aged
;
Pandemics
;
Pneumonia, Viral
;
Retrospective Studies