1.To Study the Protective Effect of Minocycline on the Model of the Cerebral Ischemia-reperfusion of Gerbils
Journal of Medical Research 2006;0(04):-
Objective To explore the protective effect mechanism of minocyline on the model of the cerebral ischemia-reperfusion of gerbils by decreasing expression of phosphorylation P38MAPK.Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids.The ultrastructural alteration in pyramidal neuron in the hippocampal CA1 and the cortex of frontal lobe at different time was observed by electron microscope.And observe the expression level of phosphorylation P38MAPK by using immunohistochemistry technique.Results ①The damage to the neunos became more and more severe as gerbils survived longer postocclusion.Minocyline treated groups were lessened obviously.② Treatment with minocycline reduced expression of phosphorylation P38MAPK with a significant difference(P
2.Characteristics of electrocardiographic parameters of the right heart in 75 cases of early newborns with varying gestational age
Chinese Journal of Applied Clinical Pediatrics 2016;31(17):1336-1338
Objective To observe the characteristics of electrocardiographic parameters of the right heart in the early newborns with different gestational age.Methods Seventy-five cases of early newborns from January 2014 to January 2015 were selected.They were divided into 4 groups according to gestational age,15 cases in group A (28-30 weeks),18 cases in group B (>30-33 weeks),17 cases in group C (>33-37 weeks) and 25 cases in group D (> 37-40 weeks).All newborns were examined by the synchronous 12-lead ECG machine,and the changes in ECG time limit detection results,QRS amplitude and RV1 amplitude were compared among groups.Results (1) There was a significant difference in P-R intervals among group A [(0.090 ± 0.011) s],group B [(0.095 ± 0.012) s],group C [(0.098 ± 0.013) s] and group D [(0.105 ± 0.014) s] (F =7.458,P < 0.05).The P-R intervals of group A,group B and group C were much shorter than those of group D (P < 0.05);the comparison of QRS duration and QTc among groups had no statistical significance (F =1.626,1.569,all P > 0.05).(2) In group A:QRS amplitude was:(4.430 ± 1.380) mV,RV1 amplitude:(0.850 ± 0.420) mV;in group B:QRS amplitude was:(4.800 ± 1.350) mV,RV1 amplitude:(1.012 ± 0.425) mV;in group C:QRS amplitude was:(5.200 ± 1.600) mV,RV1 amplitude:(1.210 ± 0.520) mV;in group D:QRS amplitude was:(5.800 ± 1.800) mV,RV1 amplitude:(1.509 ± 0.525) mV.There were significant differences in QRS and RV1 amplitude among 4 groups (F =7.002,16.870,all P < 0.05).The QRS amplitude changes in group A and group C were far less than those in group D (P < 0.05),the RV1 amplitude changes in group A,group B,and group C were far less than those in group D (P < 0.05),and there was no difference between among other groups (all P > 0.05).Conclusions There are correlations between the electrocardiographic parameters of right heart and the gestational age of newborn infants.These parameters,including the P-R intervals,the amplitude of QRS and RV1,increase with the gestational age.
3.Evaluation to the effects of a clinical pathway for acute intracerebral ischemic infarction
Jianwen GUO ; Shihong YANG ; Yan HUANG
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
0.05).Conclusion The application of clinical pathway on patients with ischemic stroke can improve the quality of medical care with reasonable cost,and it deserves to be used more widely.
4.Treatment of mycosis of maxillary sinus by nasal endoscopic surgery
Jianwen HU ; Yimin ZENG ; Guocun YANG
Chinese Journal of Minimally Invasive Surgery 2001;0(01):-
Objective To explore the curative effect of endoscopic simple maxillary ostium enlargement in the treatment of non-invasive mycosis of maxillary sinus. Methods Clinical data of 16 cases of noninvasive mycosis of maxillary sinus were analyzed retrospectively. Results The operation time was 30~120 minutes, with a mean of 60 minutes. No operative complications occurred. The hospital stay was 5~7 days, with a mean of 6 days. Histopathological examination found hyphae and spores in 16 cases, and a positive culture of Aspergillus was observed in 9 cases. Postoperative follow-up for 3~24 months (mean, 18 months) in 16 cases revealed free of symptoms in 14 cases, in which the endoscopic examination showed unobstructed ostium and normal sinusal mucosa, and recurrence in 2 cases, who underwent repeated enlargement of ostium and were re-followed for 6 and 13 months, respectively, without recurrence. Conclusions Nasal endoscopic simple maxillary ostium enlargement in the treatment of non-invasive mycosis of maxillary sinus is a radical and minimally invasive technique, but particular attention must be directed to managing the maxillary ostium.
5.A clinical observation of nasal endoscopic septoplasty
Guocun YANG ; Jianwen HU ; Yimin ZENG
Chinese Journal of Minimally Invasive Surgery 2005;0(09):-
Objective To investigate advantages of sep to plasty under nasal endoscope. Methods Septoplasty was carried out under nasal endoscope in 50 cases, including 6 cases of upper or posterior n asal septum deviation. Results The operation was completed on one session in all the 50 cases, with the deviated nasal septum being entirely c orrected. The operation time was 30~72 min (mean, 48 min). Follow-up surveys for 6~9 months (mean, 7.1 months) indicated a complete cure in 45 cases (90%) and a n improvement in 5 cases (10%). No septal hematoma, abscess, perforation, nasal cavity adhesion, or bridge collapse were encountered. Conclusions Nasal endoscopic septoplasty has advantages of short operation time, few bl ood loss, mild pain, little complications, and the possibility of nasal sinus co -operation. This technique may fully replace the traditional submucous resection .
6.Changes and its clinical significance of frontal lobe magnetic resonance spectroscopy in patients with Parkinson's disease
Haidong HUANG ; Chunmin YANG ; Jianwen GU
Journal of Clinical Neurology 2001;0(05):-
Objective To explore the changes and its clinical significance of frontal lobe magnetic resonance spectroscopy(1H-MRS) in patients with Parkinson's disease(PD).Methods 1H-MRS localized to both frontal lobe,was carried out in 36 patients with PD(14 patients with Hoehn-Yahr stageⅠ,8 patients with Hoehn-Yahr stage Ⅱ,8 patients with Hoehn-Yahr stage Ⅲ,and 6 patients with Hoehn-Yahr stage Ⅳ) and 20 age-matched healthy control subjects.The metabolite ratios of N-acetylasparatate(NAA)/creatine(Cr),and choline-containing compounds(Cho)/Cr in patients with PD on different stage were compared with those in normal control group.Results NAA/Cr ratios were reduced significantly and Cho/Cr ratios were increased significantly in both frontal lobe in patients with Hoehn-Yahr stageⅢ and Ⅳ compared to those in normal control group(P
7.Number of Fetal Nucleated Red Blood Cell in Maternal Blood and Placenta in Pregnancies with Intrauterine Growth Retardation
Jianwen ZHU ; Li ZOU ; Yu YANG
Chinese Journal of Perinatal Medicine 1998;0(02):-
Objective To examine the number of fetal nucleated red blood cell (NRBC) in maternal blood and placenta tissue in fetal growth restriction(FGR) pregnancies. Methods 20 women of 28-36 weeks' gestation at age of 21~30(including 9 FGR pregnancies)were chosen. Fetal cells were isolated from maternal blood with discontinuous density gradient centrifugation. The isolated cells were made smear and counted under the microscope; After delivery, the placenta tissue were made into sections and also counted under the microscope; To determine the origin of the NRBC , the single NRBC was analysed by primer extension preamplification (PEP) and polymerase chain reaction (PCR). Results The number of NRBC in 9 FGR pregnancy women's peripheral blood ranged from 12/7 ml~40/7 ml,(average 22.6/7 ml). The number of NRBC in the control pregnancies ranged from 0/7 ml~10/7 ml, (average 5.4/7 ml). Significant difference was shown between the two groups; The number of NRBC in 9 FGR pregnancy women's placenta tissue was significantly higher than the median value in the control pregnancies (2.8/20HP compared with 0.6/20HP, P
8.Modification with methoxy polyethylene glycol to grafts alleviates acute graft versus host disease severity in mice following haploidentical bone marrow transplantation
Guang YANG ; Suoqin TANG ; Jianwen WANG
Chinese Journal of Tissue Engineering Research 2009;13(14):2783-2786
BACKGROUND: Methoxy polyethylene glycol (mPEG) is a kind of amphotedc compound with no immunogenicity that has been used to modify various proteins covalently and to prepare versatile blood types. If mPEG modification blocks the activation of T cells in grafts, graft versus host disease (GVHD) reaction probably becomes less serious and transplantation may become successful. OBJECTIVE: To construct haploidentical bone marrow transplantation mudne model and to observe the effects of mPEG modification to grafts on acute GVHD following haploidentical bone marrow transplantation, DESIGN, TIME AND SETTING: A randomized paired design experiment was performed at the Laboratory Animal Center and Pediatric Laboratory of the General Hospital of Chinese PLA between March and November 2003. MATERIALS: Twenty 8-10 week old male BALB/cH-2d mice served as donors and sixty 8-10 week old female CBe F1H-2d/b mice served as recipients, mPEG was provided by Sigma, USA. METHODS: Mixture of donor bone marrow and spleen cells was routinely prepared. After irradiated with 60Co γ ray at a total dose of 8.0 Gy, recipient mice were randomly divided into 3 groups, with 20 rats per group: irradiation control, non-modification, and mPEG modification. Within 12 hours after irradiation, the irradiation control group was injected with 0.5 mL RPMI-1640 culture medium via caudal vein, the non-modification group was administered with 0.5 mL non-modified mixture of bone marrow and spleen cells via caudal vein, and mice from the group were given 0.5 mL mPEG-modified mixture of bone marrow and spleen cells via caudal vein. MAIN OUTCOME MEASURES: After transplantation, hematopoietic recovery, survival rate, acute GVHD and chromosomal karyotype were studied and compared with controls. RESULTS: All mice from the irradiation control groups died within 2 weeks. The 30-day survival rate was significantly higher in the mPEG modification group than in the non-modffication group (75% vs. 40%, x2 = 5.01, P= 0.025). Histopathological examinations of skin, liver and intestine showed typical signs of acute GVHD. The mPEG modification group exhibited less severe pathological presentation and lower Thomas grading than the non-modification group. Cheimedsm examination revealed complete donor-type implantation in living recipient mice at 75 days after transplantation. CONCLUSION: mPEG modification to grafts can greatly alleviate acute GVHD and enhance the survival rate of mice after haploidentical bone marrow transplantation.
9.Study on erythrocyte ATPase activity in patients with generalized tonic clonic epilepsy of different frequence seizure
Xuexu ZHAO ; Jianwen YANG ; Zuohan LI
Journal of Clinical Neurology 2001;0(05):-
Objective To study the relationship between generalized tonic clonic epilepsy of different frequence seizure and erythrocyte ATPase activity.Methods We measured Na +/K + ATPase,Mg 2+ ATPase,Ca 2+ ATPase activity of 68 patients with generalized tonic clonic seizure who were divided into 3 groups by different frequence:Status epilepsy group (SE) for 26 cases,Frequency epilepsy (FE) group for 22 cases,epilepsy (EP) group for 20 cases and the normal controls for 35 cases.Results Erythrocyte Na +/K + ATPase in SE, FE group decreased,as compared with EP group,the normal control group, there was remar kable difference( P
10.Blood transfusion of 8 earthquake victims with severe crush syndrome
Cuiying LI ; Jianwen GU ; Tao YANG
Chinese Journal of Blood Transfusion 2008;0(08):-
Objective To study the effect of blood transfusion on earthquake injuries with crush syndrome in WenChuan earthquake.Methods The amount of red blood cell suspension given to the earthquake victims were studied. The transfusion effect, Hb concentrations, and capillary hemorrhage of tissues of the injuries were also observed.Results Two victims, who underwent amputation surgery and cerebral hemorrhage operation, received a relatively small amount of transfusion, and showed a good transfusion effect and prognosis. While the other 6 victims, who underwent amputation surgery, surgical debridement, or continuous renal replacement therapy, received a large amount of blood, and showed a bad transfusion effect and prognosis.Conclusion The amount of blood transfusion in patients with crush syndrome was correlated negatively with the outcome.