Objective Nowadays ,in the field of stem cell transplantation ,improving the bone mesenchymal stem cells′ability to be implanted into target organs is still a hot topic .Objective of this study is to observe the effects of Naomaiyihao ( NMYH) capsule, a compound traditional Chinese herbal medicine invented for regulating thesea of blood in brain,and survival rate of BMSCs trans-plantaion in cerebral ischemic tissue of rat . Methods 60 rats were randomly divied into 4 group:untreated group ( serum-free L-DMEM +isotonic saline ) , BMSCs transplantation group ( cell suspension+isotonic saline ) , NMYH group ( serum-free L-DMEM+NMYH) and Combination of NMYH and BMSCs group ( cell suspen-sion+NMYH ) .A rat of model of middle cerebral artery occlusion ( MCAO) was established .The third generation BMSCs were isolated and cultured from bone marrow by the holo-bone marrow adherence , the CM-Dil labeled BMSCs were transplanted into corpora striata with stereotactic technique , the rat′s brains were removed and sliced up after 7-,14-and 21-day′transplantation .Then the survival and distribution of cells were observed under the fluorescence microscope . The expression of CD34 in the brain tissue were tested by immunohistochemical method . Results The fluorescence absorbance of combination group was 1.61 ±0.25 on day 14, increased significantly (P<0.05).when compared with BMSCs group (1.23 ±0.17). The fluorescence absorbence of combination group was 0.36 ±0.09 on day 21, increased significantly (P<0.05) when compared with BMSCs group(0.23 ±0.09).Compared with the model group ,numbers of CD34 positive cells on day 7,14 and 21 significantly in-creased in all treated groups(P<0.01).Numbers of CD34 positive cells were significantly more increased(P<0.05) in combination group than those in BMSCs group . Conclusion Survival,distribution and migration trending to boundary regions of ischemia were observed under the fluorescence microscope after transplantation .Naomaoyihao can increase blood supply around the transplanted cell region,and decrease infarct volume .Therefore it could promote BMSCs to better survive in the brain tissue .

Objective To explore the role of the autoantibodies against angiotensinⅡ type 1 receptor (ATR-1) in the pathogenesis of preeclampsia. Methods Forty normotensive and 46 pregnant women with preeclampsia were recruited in this study. Synthesized ATR-1 polypeptide fragment was used as antigens to screen the autoantibodies against ATR-1 by ELISA. The level of angiotensinⅡ was also examined. Results The positive rate of the ATR-1 antibodies and plasma level of angiotensin Ⅱ in patients with preeclampsia [63.0%(29/46) and (92.54?37.22) pmol/L] were significantly higher than those in normotensives [7.5% (3/40) and (25.75?12.33) pmol/L, P

Objective:To study influence of insulin on myocardial microcirculation of patients with coronary heart disease (CHD)complicated type 2 diabetes mellitus (T2DM)after percutaneous coronary intervention (PCI).Meth-ods:A total of 65 patients,who was confirmed as CHD by coronary angiography and fractional flow reserve (FFR)<0.75 measured by pressure guidewire,were enrolled.After drug-eluting stent implantation,index of microcircula-tion resistance (IMR)was measured.According to insulin usage or not,they were divided into insulin therapy group (insulin group,n=30)and pure oral hypoglycemic agent therapy group (pure hypoglycemic agent group,n=35). After six-month follow up,IMR was reviewed,and IMR was compared between two groups.Results:There was no significant difference in incidence rate of major adverse cardiovascular events (MACEs)between two groups,P =0.07. On six months after PCI,IMR in insulin group was significantly lower than that of pure hypoglycemic agent group [(24.5±3.1)U vs.(30.2±4.3)U,P <0.05].Conclusion:Insulin therapy can improve myocardial micro-circulation in patients with coronary heart disease complicated type 2 diabetes mellitus after PCI.

Objective To observe the expression and distribution of angiopoietin-like protein 3(ANGPTL3) in kidney of adriamycin(ADR)-induced nephrotic rats, and to explore the possible association of ANGPTL3 with the development of proteinuria and hyperlipidemia. Methods Adriamycininduced nephrotic rat models were established by a single injection of adriamycin via the tail vein. Immunohistochemical staining (IHS)and dot-ELISA were used to examine the expression and distribution of ANGPTL3 in kidney and the level of ANGPTL3 in serum of ADR rats at day 7,14, 21 and 28 after adriamycin injection. Results (1) In ADR rats, urinary protein and the level of triglyceride and cholesterol in serum increased significantly at day 14 (P

Objective To study the expression of angiopoietin-like protein 3(ANGPTL3) mRNA successively in kidney of adriamycin (ADR)-induced nephrotic rats during the development of proteinuria, and to disclose the possible assosiation of ANGPTL3 with proteinuria. Methods Adriamycininduced nephrotic rat models were established by a single injection of adriamycin via the tail vein. Glomeruli and cortex tubuli of rats were dissected by laser microdissection. Real time quantitative RT-PCR was used to study the expression of ANGPTL3mRNA in kidney of ADR rate at 7, 14, 21 and 28 days successively following adriamycin injection. Results (1) In ADR rats, urinary protein and the level of triglyceride and cholesterol in serum increased significantly at day 14 (P

ObjectiveTo investigate the association of HLA-Cw and -DRB1 alleles with psoriasis vulgaris,and to provide a clue to the study into the etiology of psoriasis.MethodsVenous blood samples were obtained from 81 patients with psoriasis vulgaris collected during 2006-2011 at the Department of Dermatology,First Affiliated Hospital of Inner Mongolia Medical College,as well as 100 age- and gender-matched healthy controls.Both the patients and controls are unrelated Mongolia in Inner Mongolia.PCR with sequence-specific primers(PCR-SSP) technique was used to genotype the HLA-Cw and DRB1 loci.ResultsThe patients with psoriasis vulgaris showed a significantly higher frequency of HLA-Cw*06(0.438 vs.0.175,Pc ＜ 0.01) and DRB1*07(0.241 vs.0.110,Pc ＜ 0.012),but a lower frequency of HLA-Cw*04(0.031 vs.0.150,Pc ＜ 0.01 ) and DRB1*04 (0.093 vs.0.235,Pc ＜ 0.01 ) than the healthy controls did.Increased frequencies of HLA-Cw*06 and DRB1*07 alleles were observed in patients with an onset before 40 years of age and those without a family history,together with a decreased frequency of HLA-Cw*04 and DRB1*04 alleles,compared with the healthy controls(Pc ＜ 0.05).The frequency of HLA-Cw*06 allele was significantly higher in patients with a positive family history and patients with an onset of no younger than 40 years of age than in the healthy controls (both Pc ＜ 0.05).ConclusionsHLA-Cw*06 and -DRB1*07 alleles may be susceptibility determinants to psoriasis vulgaris,while HLA-Cw*04 and -DRB1*04 alleles may be protective factors against psoriasis vulgaris,in Mongolia from Inner Mongolia.HLA-DRB1*07 allele may be a susceptibility gene for psoriasis,while HLA-Cw*04 and -DRB1*04 alleles may be protective factors against psoriasis,in patients with an onset before 40 years of age.

Objective To investigate the value of optimizing the thoracic CT scanning dose on reducing radiation dose.Methods 50 patients were undergone CT scanning using CARE Dose 4D technique,the mAs of each slices,CT dose index of volume(CTDIvol) and images qualities were evaluated respectively.And the results were compared with traditional thoracic CT scanning(200mAs,15.31mGy).Results Compared with routine sequence,the exposure dose of singe-slice was decreased by 48.2% when the CARE Dose 4D technique was used(maximal decreasing 84%),CTDIvol was reduced about 32.98%(maximal reduction of 56.5%),there was statistically significant difference(P

Objective To investigate the correlation between IL‐6 ,hs‐CRP and blood lipids ,blood glucose in type 2 diabetes mel‐litus(T2DM) patients complicated with coronary heart disease .Methods 64 outpatients first diagnosed T2DM complicated with coronary heart disease were selected ,56 T2DM patients and 58 health examination were as compare from 2014 January to November in my courtyard .Interleukin‐6(IL‐6) ,high sensitivity C reactive protein(hs‐CRP) and total cholesterol(TC) ,low density lipopro‐tein‐C(LDL‐C) ,blood glucose and HbA1c were detected in 3 groups of person .Results T2DM group and T2DM complicated with coronary heart disease with fasting glucose ,HbA1c ,TC and LDL‐C was significantly higher than normal group ,the difference was statistically significant(P<0 .05);The level of IL‐6 ,hs‐CRP in patients T2DM with coronary heart disease complicated was signifi‐cantly higher than that of T2DM group ,and T2DM group was higher than that of healthy group ,the differences were statistically significant(P<0 .05) .Conclusion IL‐6 and hs‐CRP can be as a specific index to predict the disease process of T2DM complicated with coronary heart disease .

Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3.Method CLCN3 transgenic mice were crossed with MMTV-PyMT spon-taneous mammary tumor model mice.The genotype was determined by PCR.The expression of ClC-3 in tissues was detec-ted by immunofluorescence and Western blot.Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established.The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis.Conclu-sions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully es-tablished.The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metas-tasis.

Objective: To compile and evaluate the Chinese version of perioperative nursing quality assessment scale, detect and verify the reliability and validity of evaluation scale, and establish a scientiifc evaluation system. Methods: We applied Tuija Leinonene and Helena Leino-kilpi scales as the theoretical framework, and established perioperative nursing quality assessment scale by translation, language study, and expert consultation. hTe questionnaire survey population was 276 patients who had surgery and 200 nurses working in the operating rooms at the 3 Affliated Hospitals of Central South University. hTe scale was tested validity and reliability. Results: Perioperative nurse quality evaluation scale consisted of 32 items and 7 dimensions, including body care, receive information, support, respect, medical staff characteristics, environment and nursing process. The test-retest reliability of scale was 0.977 for patients and 0.997 for nurses. hTe split half reliability of scale was 0.761 for patients and 0.887 for nurses. hTe Cronbach's α of total scale for the patients was 0.918, and the Cronbach's α of factors was 0.658-0.838. hTe Cronbach's α of total scale for the nurses was 0.932, and the Cronbach's α of factors was 0.650-0.836. hTe correlation coeffcient of scale for the patients was 0.363-0.603; the factors and the total correlation coeffcient was 0.535-0.788. hTe correlation coeffcient of scale for the nurses was 0.410-0.700; the factors and the total correlation coeffcient was 0.743-0.902. Conclusion: The Chinese version of the perioperative nursing quality assessment scale is a measurement instrument with a high level of validity and reliability for Chinese society and can be used to determine the quality of perioperative nursing care in our country.