1.Simultaneous Determination of Narirutin, Limonin, Honokiol and Magnolol in Zhishi Xiaopi Pills by HPLC
Jianwen XUE ; Hua LIN ; Xingzhen HUANG
China Pharmacist 2016;19(2):397-399
Objective:To establish an HPLC method for the simultaneous determination of narirutin, limonin, honokiol and mag-nolol in Zhishi Xiaopi pills. Methods:The separation was performed on a Shim-pack VP-ODS C18(250 mm ×4.6 mm,5 μm)column with the mobile phase consisting of acetonitrile–methanol(1 ∶2) (A) and water (B) with gradient elution. The flow rate was 1. 0 ml ·min-1 . The column temperature was 30℃. All the injection volume was 20 μl. Narirutin, limonin, honokiol and magnolol was de-tected at 283 nm, 210 nm, 294 nm and 294 nm, respectively. Results:Narirutin, limonin, honokiol and magnolol had good linearity within the concentration range of 5. 26-105. 20 μg·ml-1(r=0. 999 8), 7. 65-153. 00 μg·ml-1(r=0. 999 4), 6. 21-124. 20 μg· ml-1(r=0.999 3)and 6.45-129.00 μg·ml-1(r=0.999 6), respectively; the average recovery was 99.00%(RSD=0.77%), 98. 17%(RSD=1. 19%), 98. 78%(RSD=0. 86%) and 97. 90%(RSD=0. 99%), respectively. Conclusion: The method is sim-ple, rapid and reliable, which can be used for the quality control of Zhishi Xiaopi pills.
2.Relationship among CT scan and clinical findings in acute phase of subarachnoid haemorrhage and subsequent cerbral damage.
Yannan FANG ; Jianwen LIN ; Jinru LI ; Ruxun HUANG ; Wenzhong ZHENG
Chinese Journal of Nervous and Mental Diseases 2001;27(1):19-21
Objective To search the relative factors of cerebral damage, rebleeding, delayed cerebral ischaemia(DCI) and hydrocephalus after subarachnoid haemorrhage (SAH) by CT scans and clinical findings in acute phase. Methods To analyse the relationship between cerebral damage after SAH and clinical findings: CT scans resulte, age, sex, blood pressure, hyponatraemia, therapeutic methods. Results Cerebral damage were related to the pattern of distritution of SAH on brain CT and hyponatraemia. The high attack rate of rebleeding and DCI is related to presence of blood in the surface of brain, collection of blood in the ventricle, saccula aneurysms or cerebral arteriovenous malformation (AVM) (P<0.01). Conclusions To forecast of cerebral damage after SAH by study of CT scans showing and clinical findings have clincal significance. According to these findings, we may take some therapeutics to prevent the cerebral damage after SAH.
3.Application of ultrasound in shoulder lesions
Ting LIU ; Jianwen JIA ; Fajian LIN ; Xianshui FU ; Ligang CUI
Chinese Journal of Ultrasonography 2008;17(6):521-525
Objective To evaluate the value of ultrasound in diagnosing shoulder lesions.Methods Ultrasound images were obtained by two museuloskeletal sonologists in 111 patients suspected of having rotator cuff and non-rotator cuff lesions which were confirmed by following arthroscopic surgery.Results The arthroscopic findings demonstrated that there were two or more lesions in one patient in majority of patients,included 67 supraspinous tendon tears,5 infraspinous or subscapular tendon injuries,10 calcific tendonosis,2suprascapular notch cysts,34 subaeromial bursitis,1 intrabursa loose body,13 biceps longus tendon dislocation or disruption,and 21 lahrum injury.The accuracy of ultrasonogrpahy was 92% in diagnosis of supraspinous tendons tear,97% in subscapular tendon inj uries,96% in calcific tendonitis,1 00% in suprascapular notch cysts,88% in subacromial bursitis,100% in biceps longus tendon dislocation or disruption,and 81% in labrum injury.Conclusions Ultrasound can evaluate shoulder lesions effectively.
4.FULLY AUTOMATIC FRAMEWORK FOR SEGMENTATION OF BRAIN MRI IMAGE
Pan LIN ; Chongxun ZHENG ; Yong YANG ; Jianwen GU
Journal of Pharmaceutical Analysis 2005;17(1):25-28
Objective To propose an automatic framework for segmentation of brain image in this paper. Methods The brain MRI image segmentation framework consists of three-step segmentation procedures. First, Non-brain structures removal by level set method. Then, the non-uniformity correction method is based on computing estimates of tissue intensity variation. Finally, it uses a statistical model based on Markov random filed for MRI brain image segmentation. The brain tissue can be classified into cerebrospinal fluid, white matter and gray matter. Results To evaluate the proposed our method, we performed two sets of experiments, one on simulated MR and another on real MR brain data. Conclusion The efficacy of the brain MRI image segmentation framework has been demonstrated by the extensive experiments. In the future, we are also planning on a large-scale clinical evaluation of this segmentation framework.
5.MEDICAL IMAGE SEGMENTATION BASED ON A MODIFIED LEVEL SET ALGORITHM
Yong YANG ; Pan LIN ; Chongxun ZHENG ; Jianwen GU
Journal of Pharmaceutical Analysis 2005;17(1):29-32,56
Objective To present a novel modified level set algorithm for medical image segmentation. Methods The algorithm is developed by substituting the speed function of level set algorithm with the region and gradient information of the image instead of the conventional gradient information. This new algorithm has been tested by a series of different modality medical images. Results We present various examples and also evaluate and compare the performance of our method with the classical level set method on weak boundaries and noisy images. Conclusion Experimental results show the proposed algorithm is effective and robust.
6.Comparison of surgery and conservative treatment for senile dens fractures of Anderson-D Alonzo type Ⅱ or Ⅲ: a Meta-analysis
Huajun LING ; Lei FAN ; Jianwen DONG ; Weiwen LIN ; Qiyou WANG
Chinese Journal of Orthopaedic Trauma 2017;19(3):225-233
Objective To compare the operative and conservative treatments for senile dens fractures of Anderson-D Alonzo type Ⅱ or Ⅲ using Meta-analysis.Methods A literature search was conducted in PubMed,Embase,Web of Science,Cochrane Library,Wanfang Data and CNKI for studies on senile dens fractures of Anderson-D Alonzo type Ⅱ or Ⅲ from the earliest records through June 2016.The relative studies identified were screened by 2 independent authors.The quality of these articles was evaluated using modified Newcastle-Ottawa scale,and the meta-analysis was conducted using Review Manager 5.3.Results A total of 22 articles were brought into this Meta-analysis.The union rate was significantly higher in the operative group than in the conservative group[OR =0.30,95% CI(0.20,0.44),P < 0.001];the mortality in the operative group was significantly lower than in the conservative group[OR =0.61,95% CI (0.39,0.96),P=0.03];the complication rate was similar in both groups[OR=1.09,95% CI(0.76,1.57),P < 0.46].The heterogeneity of all the 3 indexes was low.In the subgroup analysis,the union rate was significantly higher in posterior operations than in anterior operations or conservative treatments (P < 0.05).Conclusion For the elderly patients with dens fracture of type Ⅱ or Ⅲ who can tolerate surgery,operative treatment may be more suitable because it can lead to much better prognosis.
7.Effect of ginsenoside Rb1 on N9 cell activation induced by oxygen deficit
Lining KE ; Wei WANG ; Jianwen XU ; Jianyin LIN
Acta Anatomica Sinica 2009;40(4):533-538
Objective To activate microglia N9 cell through the oxygen deficit, and to discuss the influence to the N9 cell by ginsenoside Rb1, laying the foundation for the basic study and the clinical medicine development. Methods Through ginsenoside Rb1 intervention, the cell morphology the proliferation ability were observed, ELISA, fluorescent probe DAF-FM DA, Griess the reagent examination, were used to measure TNF-α, the O-2 output, the NO content change, chemiluminescence, the immunofluorescence method, and plastochondria membrane potential, were carried out to detect the cytochrome C content. Results Regardless of being preventive or medical gives, ginsenoside Rb1 can decline the NO,O-2,TNF-α high expression; and reduce the plastochondria membrane potential changing, the cytochrome C redistribution. Conclusion Ginsenoside Rb1 can decline N9 cell activation to a certain extent, reduce expression of the nerve toxic factor, and to stabilize mitochondrial membrane potential and distribution of cytochrome C.
8.Inhibition Effect of a Snake Venom Metalloproteinase Inhibitor on Experimental Pulmonary Metastasis in Mice Inoculated with B16 Melanoma Cells
Jianwen XU ; Jianyin LIN ; Xu LIN ; Yuanlin QI ; Xiaoyan ZHANG ; Jinwei ZHU ; Jianshi HU
China Biotechnology 2006;0(05):-
Although there is emerging evidence that BJ46a can function as potent inhibitor of the SVMPs proteolytic activities,its anticancer effect on invasion and metastasis has not yet been evaluated.Inhibition effect of BJ46a on experimental pulmonary metastasis in mice inoculated with B16 melanoma cells at the protein level was investigated. First,BJ46a was produced in baculovirus expression system. SDS-PAGE and Western blot analysis confirmed that BJ46a recombinant protein was produced by Sf9 cells infected with high-titer viral stock. Then,recombinant fusion protein was purified by ProBondTM at the point of maximal expression. B16 cells pre-treated with recombinant BJ46a injected into C57BL/6 mice via the tail lateral vein to form experimental pulmonary metastasis model. The numbers of metastatic lesions in C57BL/6 mice changed dramatically:BJ46a different concentrations of recombinant protein group were 1.1 ? 0.83,0.9 ? 0.7,significantly lower than the control group (6.3?3.00,P
9.Glycoprotein secreted by Absidia corymbifera is associated with apoptosis of human umbilical vein endothelial cells
Fan GUI ; Youwei WANG ; Ge YANG ; Wei LU ; Jie LU ; Jianwen YANG ; Zhaochun LIN
Chinese Journal of Microbiology and Immunology 2011;31(7):582-587
Objective To partially purify the toxic factor secreted by A. corymbifera and to analyze the mechanism of A. corymbifera-induced human umbilical vein endothelial cell (HUVEC) apoptosis. Methods Glycoprotein secreted by A. corymbifera was purified by Con A Lectin chromatography. The influence of different protein fractions on HUVEC apoptosis was determined by flow eytometer. Both denaturing and nondenaturing deglycosylation of purified glycoprotein was performed and the ability of the protein moiety and carbohydrate moiety to induce HUVEC apoptosis was evaluated respectively. Activation of related caspases during A. corymbifera-induced apoptosis was analyzed by Western blot. The role of caspase-8 and -9 in HUVEC apoptosis was investigated using caspase inhibitors. Caspase inhibitors were used to stop the suppression of HUVEC viability by XTT assay. Results Flow cytometric analysis shows the total protein as well as the glycoprotein fraction of A. corymbifera may induce HUVEC apoptosis in a dose dependent manner. In contrast, similar activity was not observed in the non-glycoprotein fraction. Neither deglycosylated protein nor carbohydrate moiety is able to induce HUVEC apoptosis alone. In the apoptotic signaling pathway, caspase9, caspase-3 and cytochrome C were activated significantly, except caspase-8. Moreover, caspase-9 inhibitor, instead of caspase-8 inhibitor, completely abrogates A. corymbifera-induced HUVEC apoptosis. Caspase9 and caspase-3 inhibitors completely waived the suppression of HUVEC viability by A. corymbifera. Conclusion Glycoprotein secreted by A. corymbifera is associated with HUVEC apoptosis. Intact glycoprotein is essential for the apoptotic progress. Intrinsic apoptotic signaling pathway mediates A. corymbifera-induced HUVEC apoptosis.
10.Screening significance of transcranial Doppler for patients with patent foramen ovale induced cerebral embolism
Hongling ZHAO ; Hong WANG ; Cui WANG ; Jianwen LIN ; Tieping FAN ; Lili XIE ; Suping WANG
Chinese Journal of Postgraduates of Medicine 2012;35(18):12-14
Objective To investigate the screening significance of transcranial Doppler (TCD) for patients with patent foramen ovale ( PFO) induced cerebral embolism and to improve the treatment aiming at the causes.Methods Thirty-six patients of less than 60 years old with unknown-cause cerebral embolism and transient ischemic attack were enrolled.Besides conventional examination,the 24 h Holter,transthoracic echocariiography,carotid artery Doppler ultrasonography (CAU),routine TCD,TCD foaming test,transesophageal echocardiography (TEE),CT angiography (CTA) of head and neck,ultrasonography of vein in bilateral lower limbs were examined in all patients.Results All the patients showed no atrial fibrillation.No structural heart disease and intracardiac occupying lesion were shown in transthoracic echocardiography.No carotid stenosis was shown in carotid artery Doppler ultrasonography.Routine TCD showed no intracranial artery stenosis.No thrombus was found in ultrasonography of deep veins in bilateral lower limbs.CTA of head and neck showed everything was normal.Fourteen patients were observed with right-to-left shunt in heart by TCD foaming test,including 10 cases with microbubble signals(MBS) in 10 s,and 4 cases with MBS after Valsalva maneuver.Among 14 patients,12 patients were proved PFO by TEE.Conclusions PFO is one of the causes of cardiogenic cerebral embolism.TCD is accurate and reliable for the screening of PFO.