AIM: To explore the effect of low concentration of N-methyl-N-nitro-N-nitroguanidine (MNNG) on p38MAPK, and the function of glutathione (GSH) on p38MAPK. METHODS: Western blotting was applied to detect the p38MAPK phosphorylation in MNNG treatment group and control group. To study the effect of GSH on MNNG-regulated p38MAPK activity, the intracellular GSH level was reduced by pretreatment of L-buthionine-S, R-sulfoximine (BSO). Assuming the absorbance of band in control group as 1.0, the relative P/T values of the treatment groups were calculated, with the “P” served as absorbance values of phospho-p38MAPK and the “T” as absorbance values of total p38MAPK. RESULTS: In BSO pretreated groups,the relative P/T in samples treated with MNNG for 2.5 h was 0.84, and became 2.19 with 3 h more incubation in the fresh medium. CONCLUSION: GSH deletion increases the activity of p38MAPK in MNNG treatment.

Objective To explore the expression and significance of MTSS1 ( metastasis suppressor 1) in hepatocellular carcinoma.Methods MTSS1 expression was detected by immunohistochemistry in hepatocellular carcinoma, liver cirrhosis and normal liver tissues.Single-factor analysis was used to study the relationship with clinicopathological factor.Correlations between MTSS1 expression and TNM stage were analyzed with Spearman rank correlation analysis.Postoperative 5-year survival was evaluated using Kaplan-Meier survival curve analysis.Results The expression of MTSS1 in hepatocellular carcinoma was higher than normal liver tissue ( U = 168.000, P ＜ 0.05), and liver cirrhotic tissue ( U = 106.000, P ＜ 0.05); MTSS1 expression was correlated with TNM stage of hepatocellular carcinoma patients, lymph vascular invasion and tumor capsule ( separately U = 259.000, 258.500, 202.000, all P ＜ 0.05).MTSS1 expression in hepatocellular carcinoma was not correlated with patients age, gender, tumor size, AFP level, and hepatitis B surface antigen.MTSS1 expression and TNM stage of liver cancer patients was negatively correlated ( rs = - 0.383 , P ＜ 0.05 ).Postoperative 5-year survival of hepatocellular carcinoma patients with MTSS1 positive expression was significantly poorer than patients with negative and weakly positive expression (respectively 34.1% and 52.3% , x2 =6.386, P ＜ 0.05).Conclusions MTSS1 high expression may play an important role in the early hepatocellular carcinoma progression, indicating a poor prognosis.

[Objective]To discuss the technique and outcome in the treatment of old nonunion of long bone fracture by using autologous fibula plate alone or combination with internal fixator plates.[Method]Fifty-seven patients(41 males and 16 females;average age of 41.6 years)with nonunion of long bone fracture were recruited.Of them 18 had short deformity(average 2.6 cm)and 12 had angled deformity(average 15?).Average time was 12 months(6-36 months).The sclerosed cortex was removed at both ends of the nonunion long bone.A suitable length of fibula plate were transplanted by screw with or without internal fixator plates.[Result]The follow-up average time was 3 years(ranged,1 to 6 years).At 1,3,6,12,24 months after operation,the outcome was analyzed by radiography.Bone healing was found in all 57 patients,with an average of 15 weeks(8-24 weeks).All the patients exhibited a good function in walking and burdening.No nonunion occurred.[Conclusion]Autologous fibula plate with or without internal fixator plates for nonunion of long bone fracture can facilitate healing and early functional recovery.This therapeutic method is an ideal technique in treating patients with nonunion of long bone fracture.

Baizi Yangxin Pian is a reformed preparation of Baizi Yangxin Wan (bolus) listed in the Pharmacopoeia of People's Republic of China and is considered as a new drug of 4th class. According to the requirement of Regulations for New Drug Approval, its pharmacodynamics and acute and long-term toxicity tests were observed. It was shown that this preparation has a tranquilizing effect which is stronger than Baizi Yahgxin W an. LD_(50) is greater than 37.5g/kg. No toxic effect is revealed in rats after its long-term administration (9g/kg, 4.5g/kg and 3g/kg).

[Objective]To observe the influence of Yigu oral liquid on inhibiting the pain in ovariectomized rats by detecing the pain threshold, bone densi-ty, serum inflammatory factor, vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63).[Method]80 SD rats were randomly di-vided into four groups of sham group, Yigu oral liquid group, Miacalcic group,control group. There were 20 in each group.Yigu oral liquid group,Mia-calcic group,control group were ovariectomized,sham group underwent sham operation. After confirming the successful model of osteoporosis, sham group was administrated with saline, Miacalcic saline group was administrated with Miacalcic by intramuscular injection ,Yigu oral liquid group was administrated with Yigu oral liquid ,saline and control group was administrated with saline.The indicators of pain threshold,bone density,vascular endothelial function (ET) and platelet activating capacity(CD62p and CD63), serum inflammatory factors as COX-2, PGE2, cAMP were respectively detected. [Results] The levels of peripheral inflammatory factors as COX-2, PGE2, cAMP , vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63)of Yigu oral liquid group decreased significantly compared with the control group( P<0.05). The level of pain threshold and bone density of Yigu oral liquid group improved significantly compared with the control group(P<0.05).[Conclusion]Yigu oral liquid can inhibit osteoporosis pain by improving the level of pain threshold ,bone density,and decreasing the levels of COX-2, PGE2, cAMP, vascular endothelial function(ET) and platelet activating capacity (CD62p and CD63).

Seventy-three patients with bed sores were treated by warming moxibustion. Of the 73 cases, 58 cases were cured, 12 cases improved and 3 cases ineffective, with an effective rate of 95.9%.

Objective To study the effect of mitochondrial permeability transition(MPT) after ischemia/reperfusion(I/R), and to find the relationship between MPT and apoptosis of hepatocytes in rats. Methods SD rats were divided into three groups:sham operation group, I/R group, and I/R+CsA group. A model of hepatic I/R in rats was made according to the method of Nauta. Active caspase-3 in cytoplasm was examined by immunohistochemistry, and the content of cytochrome C protein was evaluated by Western blotting. The incidence of apoptic hepatocytes was determined by TUNEL method. Cyclosporine A(10 mg?kg -1?d -1) was used as an inhibitor of MPT owing to its inhibitive effect on mitochondrial permeability transition pore(PT pore), and the influences of CsA were analyzed. Results Pretreated with CsA ameliorated injury after reperfusion. The number of apoptotic liver cells was decreased. CsA significantly prevented the release of cytochrome C from mitochondria, demonstrating its effect on MPT through preventing PT pore from opening. Conclusion MPT may play a key role in the apoptosis of liver cell after I/R. CsA, a powerful inhibitor of PT pore, is capable of abating the effect of MPT, reducing the release of cytochrome C, retarding the activation of caspase-3 and hence ameliorates apoptosis of hepatocytes following I/R procedure.

0.05).The average fading time of the lymph nodes stained by PB and MB+PB was 13.06min and 82.13min,we observed the lymph nodes stained by MB 120 min,they were still stained deeply.There were dramatically differences among three groups(P=0.000).Conclusion Application of MB and PB possess their advantage and disadvantage in sentinel node biopsy.The mixture of patent blue and methylene blue could overcome the shortage of the two vital dyes in the experimental study and be an ideal tracer for the use.

Objective To study cortactin function in cancer cell endocytosis.Methods We applied cortactin siRNA interference to MDA-Mb-231,a human breast adenocarcinoma cell line in which cortactin was over-expressed,and introduced anti-cortactin immunoreagents into the cells with BioPorter system to interfere with cortactin function in vivo.Capture-ELISA assay was used to measure transferrin uptake.We used immunoblot assay to assess the effect of cortactin knock-down and immunoflurescence microscopy to examine the effect of cortactin down-regulation on transferrin uptake.Results Interference of cortactin function in cells resulted in impairment of transferrin endocytosis.Transferrin fluorescent intensity in cytoplasm in cortactin siRNA treated-cells was significantly reduced in comparison to that of mock-treated cells.Less than 50% of cells subjected to cortactin siRNA treatment had normal transferrin uptake.Endocytosis in MDA-Mb-231 cells introduced with cortactin antibodies was impaired as well,showing a 30%～ 60% reduction in transferrin uptake.Conclusion Crtactin,an actin-binding protein,plays an essential role in cell endocytosis.

ObjectiveTo determine the relationship between the expression of bax and bcl-2 gene in mucous adenocarcinoma of rectum and patient′s prognosis.MethodsImmunohistochemical method was used to evaluate monoclone antibody of bax and bcl-2 protein in 38 cases of mucous adenocarcinoma of the rectum. ResultsThe positive-staining rate of bax was 77.8% in Dukes′ A and B stage compared to 45.0% in Dukes′ C stage( ? 2=4.260,P=0.039).The intensity of positive-staining of bax over bcl-2 was significantly correlated with tumor stage(P=0.04) and the survival (P=0.001).ConclusionThe expression rate of bax and bcl-2 gene was very high in mucous adenocarcinoma of large bowel, and it might have a prognosis implications in patients with colonic mucous adenocarcinoma.