Objective To investigate the inhibitive effect of Konjac polysaccharide(KP) on intestinal absorption function of mice and its mechanism.Method Mice were divided into normal control(N),high fat(HF),and high,middle,low dose KP(KPH,KPM,KPL) treated groups.Intestinal mucosa Na+-K+-ATPase activity was determined by UV spectrophotometry,blood glucose was determined by fast meter after twenty days.Body weight,fecal wet weight and dry weight were also examined.Results Na+-K+-ATPase activity of HF and KPH groups were 16.2?1.48 and 11.2?1.10 ?mol Pi/(mg prot?h),respectively.Body weight and postprandial blood glucose were 34.3?2.07 g,7.5?1.15 mmol/L and 28.1?1.95g,4.8?0.73 mmol/L,respectively.The differences between two groups were significant(P

Objective To study the clinical effect of small incision cataract extraction in the treatment of acute angle-closure glaucoma with cataract.Methods Fifty-eight patients with acute angle-closure glaucoma combined with cataract were selected,and according to the digital table they were randomly divided into two groups:control group and observation group.The patients in the control group were treated by conventional method.The patients in the observation group were treated with small incision cataract.The changes of intraocular pressure,anterior chamber depth and visual acuity before and after treatment were observed.The incidence of complications and the satisfaction of treatment were compared between the two groups.Results There were no significant differences in IOP,visual acuity and anterior chamber depth between the two groups before treatment(all P>0.05).After treatment,the intraocular pressure,anterior chamber depth and visual acuity of the observation group were (10.2±4.0)mmHg,(3.3±0.5)mm,(0.85±0.08),respectively,which of the control group were (17.5±5.0)mmHg,(3.2±0.5)mm and (0.50±0.06),respectively.The improvement of intraocular pressure and anterior chamber depth of the observation group was more significant than the control group(t=0.120,0.520,1.012,all P<0.05).The satisfaction rate of the observation group(96.6%) was higher than that of the control group(75.8%)(χ2=9.823,P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).Conclusion Small incision cataract extraction is an effective treatment for acute angle-closure glaucoma complicated with cataract,and the postoperative complications are less.The patients are satisfied with the treatment and deserve to be popularized.

Objective To optimize the processing parameters for the extraction of chlorogenic acid in Fule Granula by Box-Behnken experimental design.Methods The content of chlorogenic acid in Fule Granula was determination.The separation was performed on a Agilent ZORBAX Eclipse XDB C18 column(4.6 mm ×250 mm, 5 μm), and column temperature 30 ℃.Aetonitrile-0.2% H3PO4 was used as the mobile phase.The detection wavelength was at 327 nm.A three-factor and three-level Box-Behnken experimental design was employed to investigate effects of extraction time, solid-liquid ratio and extraction times on composite score of extracting amount of chlorogenic acid.Results Optimum process conditions were as follows:12 times the amount of water extracted three times, each time 60 min; chlorogenic acid extraction rate of 3.379%.Conclusion This optimized extraction technology has good predictability by Box-Behnken experimental design, and overall desirability, it provides a reference for application of Fule Granula.

Objective To study the effect of Konjac polysaccharide (KP) on motilin (MOT) and somatostatin (SS) of gastrointestinal tissue in mice. Methods The mice were divided into four groups randomly. Normal control group (NC) was given normal feed. High, middle, low dose KP treated groups (KPH,KPM,KPL)were given KP 0.8, 0.4, 0.2g per 100g normal feed. The level of motilin and somatostatin of gastrointestinal. tissue was determined simultaneously by radioimmunassay, and blood glucose was determined by blood glucose analyzer after 20 d. Body weight, adipose tissue and wet weight of stool were also examined. Results The level of motilin of stools of KPH group was 8.26?0.53ng/g ,which increased compared with NC group.Somatostatin was 207.32?21.89 ng/g ,which decreased compared with NC group. The differences between two groups were significant (P

Objective To explore the correlations between the levels of serum 25 ( OH) D and age, blood sugar, blood lipid, blood pressure and insulin levels in patients with subcortical arteriosclerotic encephalopathy . Methods A total of 100 patientswith subcortical arteriosclerotic encephalopathy ( SAE) and 50 healthy persons were enrolled in the study.ELISA was adopted to determine serum 25(OH)D levels in two groups.Meantime, fasting glu-cose, blood lipid, blood pressure and insulin levels were measured and compared between two groups .Results The systolic blood pressure , diastolic blood pressure , fasting glucose , total cholesterol and insulin levels in SAE patients were significantly higher than those in the healthy control (p<0.05).The serum 25(OH)D levels in SAE patients were significantly lower than that in the healthy control (p<0.01).There were no significant differences in triglycer-ide and lipoprotein between two groups (p>0.05).The serum 25(OH)D levelswere negatively related with age, SBP, DBP and fasting glucose(p<0.05,p<0.01) but not correlative with TC, TG, LDL, HDL, and insulin levels in SAE patients.Conclusion The serum 25(OH)D levels in SAE patients are reducedby playing a regulative role in the occurrence and development of SAE throughaffectingthe energy metabolism .

Objective To explore the effects of the vagus and its transmitter on cerebrogenic multiple organ dysfunction syndrome induced by cerebral hemorrhage(CH) in rats. Methods 80 wistar rats were randomly divided into sham-operative group (n=10),subdiaphringmatic vagotomy (SDV) group (n=10),CH group (n=20), CH+SDV group (n=20) and CH+SIV group (n=20).Rat CH model was made by stereotaxis injection of collagenase and heparin sodium in caudate nucleus. The success full CMODS rat model was characterized by the abnormality of hepatic and renal function,myocardium enzyme, blood routine and vital sign of rat chalked standard. The rats of CH+SDV group were practiced SDV operation 4 weeks before the model was made. CH+SIV group were put electrostimulation of vagus after the mode was made for 6 times. The stimulus continued 20 min with 4 h interval. All rats were executed 24 h after CH. The pathological change of main organs (liver, kidney, lung, small intestine),blood routine were tested. Myocardium enzyme,hepatic and renal function were examinied. Meanwhile, the expression of FOS,CHAT and VIP in the medullary visceral zone of the rats were examined with immunohistochemistry,compared with the sham-operative group and SOV gruop. Results Abnormality of hepatic and renal function,myocardium enzyme and the pathological change of liver,kidney,small intestine CH+SDV group,CH+SIV group and those of CH group outweigh than sham-operative group and SDV group(all P

Objective To study the radiographic features of pulmonary sclerosing hemangioma (PSH), and to correlate the imaging with the pathologic findings so as to improve the imaging diagnostic abilities. Methods The clinical features of 45 patients with pathology proved PSH between 1976 and 2002 were reviewed. Of the 45 patients, there were 3 men and 42 women, their age ranged from 18 to 67 years (median age, 49 years), and 19 patients were asymptomatic. 20 patients had chest radiography and CT scans. The radiographic findings were studied retrospectively and were correlated with that of pathology. (7 patients) underwent unenhanced CT and 13 patients had enhanced CT. Results Of the 20 patients who had chest X-ray, 18 patients presented as a solitary, round or oval shaped lesion with well-defined margin. On CT scans, the greatest dimension of the lesion ranged from 1.9 cm to 7.0 cm. A homogeneous soft-tissue attenuation was revealed on unenhanced scans. Homogeneous enhancement was detected in 9 patients and heterogeneous enhancement in 4 patients on enhanced CT. Cystic change was found in 1 patient showing lower attenuation than the enhanced solid tumor, and calcification was found in 5 patients. Correlation of radiographic and pathologic findings indicated that higher attenuation areas corresponded to the angiomatous areas. Conclusions PSH should be considered with the following features: (1) female patients between (40-60) years of age; (2) well-defined round or oval shaped lesion on chest radiography; (3) a homogeneous soft-tissue mass on unenhanced CT; cystic-like area within the tumor and/or calcification can occasionally be found; (4) homogeneous or heterogeneous enhancement after contrast administration.

PBL,problem based learning,is a kind of teaching model for resolving problems. Based on the present teaching situation of our university,my experience in NewYork University and my teaching experience for overseas students,this article elucidated the importance and necessity of PBL teaching model in overseas student education and put forward a suitable PBL teaching method.

Rotundine (1 micromol L(-1)) was incubated with a panel of rCYP enzymes (1A2, 2C9, 2C19, 2D6 and 3A4) in vitro. The remained parent drug in incubates was quantitatively analyzed by an Agilent LC-MS. CYP2C19, 3A4 and 2D6 were identified to be the isoenzymes involved in the metabolism of rotundine. The individual contributions of CYP2C19, 3A4 and 2D6 to the rotundine metabolism were assessed using the method of total normalized rate to be 31.46%, 60.37% and 8.17%, respectively. The metabolites of rotundine in incubates were screened with ESI-MS at selected ion mode, and were further identified using MS2 spectra and precise molecular mass obtained from an Agilent LC/Q-TOF-MSMS, as well as MS(n) spectra of LC-iTrap-MS(n). The predominant metabolic pathway of rotundine in rCYP incubates was O-demethylation. A total 5 metabolites were identified including 4 isomerides of mono demethylated rotundine and one di-demethylated metabolite. The results also showed that CYP2C19, 2D6 and 3A4 mediated O-demethylation of methoxyl groups at different positions of rotundine. Furthermore, the ESI-MS cleavage patterns of rotundine and its metabolites were explored by using LC/Q-TOF-MSMS and LC/iTrap-MS(n) techniques.

Objective To investigate the effect of propofol combined with remifentanil or sufentanil on cognitive function in patients undergoing awake craniotomy. Methods Sixty ASA Ⅰ or Ⅱ neurosurgical patients undergoing resection of glioma in cerebral cortical functional area were divided into 2 groups by random digits table: propofol + remifentanil (group RF, 30 cases) and propofol + sufentanil (group SF, 30 cases). Scalp nerve block and local infiltration of incision and dura mater were performed in both groups with 0.5% ropivacaine. Propofol, remifentanil and sufentanil were administered by target controlled infusion. The target plasma concentration of remifentanil was set at 1-2 ng/ml and that of sufentanil at 0.1-0.2 ng/ml,propofol was set at 3-6 μg/ml at open skull stage. The patients were inserted laryngeal mask and mechanically ventilated. Bispectral index (BIS) was monitored as the depth of anesthesia. Mini-mental scale examination (MMSE) was investigated at the time of preoperative,intraoperative wake-up after the patients had been targeted capacity. Results Blood concentration of propofol in group RF was (1.10 ± 0.06)μg/ml, group SF was (0.98 ± 0.05)μ g/ml in patients during intraoperative wake-up. BIS in group RF changed from 46.4 ± 2.5 to 90.8 ± 3.2 during wake-up, group SF from 44.8 ± 2.1 to 89.9 ± 3.2. The cognitive function score was not significantly different at the time of preoperative and intraoperative assessment. Conclusion Propofol combined with remifentanil or sufentanil has no effect on cognitive function for the patients undergoing awake craniotomy.