The studies on the preparation technology,quality control and pharmacodyrnamics of Xi- aoyaosan Oral Liquid have been carried out. The experiment results suggested that,using the color reaction, TLC Identification and TLC-scanner could effectively control the intrinsic quality of oral liquid. Both possessed practical availability and their operations were simple Meanwhile,pharmacodynamic study also indicated that oral liquid could increase the peristalsis of stomach and bowel,the amount of consumed food and the body weight of mice,which was in conformity with the indication and efficacy of the original prescription.

The preparation process of Xiaoyaosan Oral Liquid was investigated with the orthogonal test. As a result,the amount of water added was a main factor influencing the dip-extraction effect of crude Chinese medical materials when the oral liquid was made with the method of distillation and warm dip. It was suggested that the following process would be the best to ensure the extraction of active compo- nents:adding 10 times amounts of water,then dipping and extracting for 1.5h at 90℃ and repeating 3 times.

Objective To investigate the Helicobacter pylori (Hp) infection in the immediate relatives of patients with gastric cancer.Methods One hundred and eighty two immediate relatives of 86patients with gastric cancer and 126 age and sex-matched immediate relatives of 46 dyspeptic patients were enrolled in the study from March 2010 to March 2011.C14 or C13 urea breath tests were performed and for those with Hp-positive results serological typing was further performed.Results One hundred and forty two out of 182 immediate relatives of gastric cancer patients were Hp positive with a Hp infection rate of 78％,which was significantly higher than that of immediate relatives of dyspeptic patients ( 78.0％ vs 47.6％,x2 =30.5,P ＜ 0.05,P =0.006 ).The average age of former was significantly higher than that of latter (46 ±6 y vs 38 ± 6 y; t =13.34,P ＜ 0.05).The rate of serological type Hp-1 in immediate relatives of cancer patients was significantly higher than that of those of dyspeptic patients (68.3％ vs 38.3％,x2 =14.28,P ＜0.05 ).The infection rate of children,both parents of whom were infected with Hp and only one parent infected with Hp (52.8％,43.2％,respectively ) was higher than that of children whose parents were not infected with Hp ( 13.3％ ) ( x2 =6.80,P ＜ 0.01 and x2 =4.35,P ＜ 0.05 ).Conclusions The Hp infection rate is high in immediate familial members of gastric cancer patients and the predominant serological type of Hp is Hp-1.

Objective To evaluate single balloon dilatation and placenment of the stent for the treatment of Budd Chiari Syndrome.Methods Thirty four patients with Budd Chiari Syndrome underwent covagraphy catheterized through superior and inferior vena cava (IVC) simultaneously and single balloon dilatation of IVC and placement of metallic stents.Results Puncture and dilatation were successful in 33 patients.The obstructed segments of IVC were dilated to 10～20 mm in diameter.Nine metallic stents were placed in 9 cases. The caval pressure below obstruction were reduced from(2.71?0.78) kPa to(1.98?0.85) kPa in average.Conclusions Covagraphy catheterized through superior and inferior vena cava (IVC)simultaneously reveals the site and length of the obstruction clearly.Insertion of large single balloon is technically simple and dilatation is definite.Restenosis in segmental type may be prevented by stent insertion.

Objective To evaluate the value of CT examination in localized diagnosis of primary mesenteric occupancy lesions.Methods CT findings in 35 cases of primary mesenteric occupancy lesions proved operatively and pathologically were analysed.Results Accuracy and postive forecast rate of localization of lesions by CT were 62.9% and 78.6% respectively.The findings of localized diagnosis included:(1)The relation between the occupancy lesions and intestines was closed,the situs was circled by intestines which were fixed and there was no filling defect in 25 cases.(2)The main retroperitoneal organs were distorted and their axes were rotated by the lesions in 15 cases.(3)There was fat space between the lesions and the retroperitoneal organs and the abdomen walls in 11 cases.(4)The main blood vessels of abdomen were compressed by the lesions or the lesions circled by vessels in 9 cases.The localized accuracy was lower when the lesions were larger.Conclusion CT is useful for localization of primary mesenteric occupancy lesions.CT combined with other imaging data and clinical symptoms can improve CT localized accuracy.

Objective To investigate the features of the enhancement of SHCC at triple-phase spiral CT scanning and compare its sensitivity in different phase.Methods The pre- and post-contrast CT scanning of the entire liver in 70 patients with SHCC was carried out with a helical CT scanner. The contrast material was infused at a rate of 3ml/s, followed by sequential arterial-, portal- and equilibrium- phase scans of the liver. The number of the detected lesions was calculated in each phase, the characterization of lesions in each phase was observed.Results Of the 82 detected SHCC, the sensitivity in three phases was 92.68%, 70.73% and 73.17% respectively. The sensitivity increased significantly to 95% by combining the detection of three phases. Conclusion Triple-phase contrast enhanced spiral CT can fully demonstrate the patterns of SHCC and improve its detection rate.

Objective To study the CT findings of late onset intracranial hemorrhage in vitamin K(Vit K) deficiency.Methods 56 cases of late onset intracranial hemorrhage in Vit K deficiency proved clinically were presented.There were 42 male and 14 female,age ranging 28～60 days.Brain CT scans were obtained in all 56 cases. Results Subarachnoid hemorrage(SAH) in 33 cases,subdural hemorrage(SDH) in 32 cases,intracerebral hemorrage(ICH) in 19 cases and inraventricular hemorrage(IVH) in 8 cases were found.The hemorragic amount was ranged 2～150 ml.Simple hemorrage was demonstrated in 26 cases,and multiple mixed hemorrage in 30 cases.The cerebral edema was displaied in 16 cases.Conclusion SAH,SDH and multiple mixed hemorrage are common seen in the late onset intracranial hemorrage in Vit K deficiency.

Purpose:To investigate the effects of octreotide on TGF-? autocrine in human hepatocellular carcinoma cells SMMC-7721 and TGF-?-induced cells proliferation. Methods:The expression of TGF-?in SMMC-7721 was determined by radioimmunoassay and reverse-transcriptase polymerase chain reaction(RT-PCR). The expression of epidermal growth factor receptor (EGFR) in the cells was determined by immunohistochemistry method and RT-PCR. The proliferative activity of the cells was evaluated by flow cytometry and colony-forming assay. Results:The content of TGF-?was significantly attenuated by octreotide and the inhibitor rate was 15.0%~26.7%. TGF-?mRNA index was decreased by octreotide.TGF-?increased the expression of EGFR both in mRNA and protein level,while octreotide inhibited the expression induced by TGF-?. Proliferative index (PI) and colony-forming rates were obviously lower in octreotide and TGF-?-treated cells than those in TGF-?-treated cells. Conclusions:There exists a TGF-? autocrine loop in human hepatocellular carcinoma cells SMMC-7721. octreotide could inhibit TGF-? autocrine in the cells, and consequently exerts an inhibitory effect on cell proliferation.

Asialogycoprotein receptor (ASGPR) is a specific receptor of mammalian hepatocytes,exclusively up-take the glycoprotein in the blood.The number of ASGPR correlates significantly with liver function,and the reduction of the number of ASGPR suggest liver dysfunction.Decreased ASGPR in patients with liver cirrhosis or portal hypertension or hepatocellar carcinoma,lead to varying degrees of liver dysfunction,which made it more susceptible to post operative complications.liver ASGPR scintigraphy with Technetium-99 m DTPA-galactosyl human serum albumin (99m Tc-GSA),combinating with single photon emission computed tomography (SPECT) technology can assess the functional reserve of remnant liver and predict incidence of postoperative complications,then assist to evaluate the use fulness for clinically surgical decisions.Current situation and progress of 99mTc-GSA SPECT imaging in hepatic surgery were reviewed in the paper.

Tumor metastasis and recurrence had become a key to curative effect and long-term survival,and it's hotspot of current clinical oncology research.Differential expression of proteomics between cancer and normal tissue can be used for discovering new tumor markers.Clinical detection of liver cancer-related protein molecules was useful to monitor and assess early diagnosis,recurrence and prognosis of hepatocellular carcinoma.Herein,this article reviewed the research progression of protein molecules in hepatocellular carcinoma.