Objective:To evaluate the expression and significance of Bcl-2 ,Bad gene and its relationship with prognosis of breast cancer.Methods:Immunohistochemical technique was used to detect the expression of Bcl-2 ,Bad gene in 10 cases of normal breast tissue,10 breast fibroadenoma tissue,40 young human breast carcinoma tissue and 40 old human breast carcinoma tissue.The expression of ER and PR in 80 cases of human breast carcinoma tissue were also detected.Results:The expression of bcl-2,bad gene decreased in the development of breast cancer(P0.05).Conclusion:The expression of bcl-2 and bad can be used as predicted factors of breast cancer.Detection of the expression level of the bcl-2 protein,in particular the combined detection of the expressions of bcl-2 and bad as well as ER and PR is helpful in forecasting the prognosis of breast carcinoma and instructing the treatment.

AIM: To explore the effects of ethanol on replication and expression of HBV, so as to effectively guide the anti HBV treatment in clinic. METHODS: 2.2.15 cells were cultured in vitro. Ethanol of different concentrations was added into the supernatant and 3TC was used as the positive control. And there was no drug added in the negative control group. Effect of ethanol on HBV expression and replication was examined and the expression of antiviral gene MxA was also tested. RESULTS: Ethanol promoted the expression of intracellular HBsAg, HBeAg and intracellular HBcAg. But 3TC revealed no significant effect on all of them. Southern blotting showed that ethanol at 200 mmol?L -1 enhance the replication of HBV DNA, but 3TC inhibited the replication of HBV DNA at 2 ?mol?L -1 .Ethanol at 40 mmol?L -1 promoted the expression of MxA, while 3TC at 2 ?mol?L -1 also stimulated the expression of MxA. CONCLUSION: Ethanol can induce the overexpression of HBsAg, HBeAg and HBcAg, and enhance the expression of antiviral gene MxA which may increase the activity of enzymes of synthetic metabolism. Ethanol can promote replication of HBV DNA, but the mechanism is unclear and need to be studied.

Objective To investigate selective pregnancy reduction in second-trimester and its notices. Methods Selective pregnancy reduction in 19 cases of multiple pregnancy during 12～25 gestational age was performed,including 6 cases of twins,1 cases of triplets,1 case of quadruplet and 1 case of sextuplet. Ultrasonographically guided transabdominal intracardiac injection of high concentrate potassium chloride (KCl) solution was used. Results Performances of selective feticide in all twins were successful,except one monochorionic twins. In 11 cases of triplets,one case was aborted,one case was monozygotic triplet. Selective feticide was performed successfully in 7 of the rest 9 cases and the quadruplet Two cases were aborted after the procedure. The sextuplet was aborted after feticide. Conclusion Multifetal pregnancy reduction can be successfully performed in the second trimester without severe maternal complications. But abortion might occur afterwards. It is possible to reduce the abortion rate if monozygotic twin or triplet can be excluded before feticide,choosing the gestational sac which near the fundus,reducing the amniotic fluid volume of the target fetus or postponing feticide.

Objective To investigate the features of the enhancement of SHCC at triple-phase spiral CT scanning and compare its sensitivity in different phase.Methods The pre- and post-contrast CT scanning of the entire liver in 70 patients with SHCC was carried out with a helical CT scanner. The contrast material was infused at a rate of 3ml/s, followed by sequential arterial-, portal- and equilibrium- phase scans of the liver. The number of the detected lesions was calculated in each phase, the characterization of lesions in each phase was observed.Results Of the 82 detected SHCC, the sensitivity in three phases was 92.68%, 70.73% and 73.17% respectively. The sensitivity increased significantly to 95% by combining the detection of three phases. Conclusion Triple-phase contrast enhanced spiral CT can fully demonstrate the patterns of SHCC and improve its detection rate.

Objective To study the CT findings of late onset intracranial hemorrhage in vitamin K(Vit K) deficiency.Methods 56 cases of late onset intracranial hemorrhage in Vit K deficiency proved clinically were presented.There were 42 male and 14 female,age ranging 28～60 days.Brain CT scans were obtained in all 56 cases. Results Subarachnoid hemorrage(SAH) in 33 cases,subdural hemorrage(SDH) in 32 cases,intracerebral hemorrage(ICH) in 19 cases and inraventricular hemorrage(IVH) in 8 cases were found.The hemorragic amount was ranged 2～150 ml.Simple hemorrage was demonstrated in 26 cases,and multiple mixed hemorrage in 30 cases.The cerebral edema was displaied in 16 cases.Conclusion SAH,SDH and multiple mixed hemorrage are common seen in the late onset intracranial hemorrage in Vit K deficiency.

Objective To investigate the effect of alprostadil injection on serum tumor necrosis factor ( TNF-α) ,β2-microglobulin (β2-MG) and soluble interleukin 2 receptor (sIL-2R) levels in adjunctive treatment of patients of severe hepatitis with liver and kidney syndrome.Methods A total of 60 patients of severe hepatitis with liver and kidney syndrome were collected and randomly divided into experimental group and control group with 30 cases in each group.The two groups were treated by conventional therapy, patients in the control group were treated by continuous renal replacement therapy, patients in the experimental group were treated on the basis of the control group with alprostadil injection in adjuvant therapy for 3 weeks.The serum biochemistry and liver and kidney function and clinical curative effect were detected.Results Compared with the control group, the serum TNF-α, β2-MG and sIL-2R levels of the experimental group were lower (P<0.05); alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirnbin (TBiL) levels were lower (P<0.05), albumin (Alb) level and prothrombin time activity (PTA) were higher (P<0.05), serum creatinine (Scr), urea nitrogen (BUN), CysC and 24h urine protein were lower (P<0.05), 24h urine volume was higher (P<0.05); efficacy in experimental group was better than control group (Z=-2.321,P=0.020).Conclusion Alprostadil injection in adjunctive treatment of severe hepatitis with liver and kidney syndrome patients has significant effect and high safety, could significantly reduce the serum TNF-α, β2-MG and sIL-2R levels.

Cell culture technology is the most commonly used method in the in vitro experiments at present. However, monolayer cell culture technology has been unable to meet the demand of the researchers. This is because that monolayer cell culture cannot mimic the cellular environment in which multiple cells interact with each other in the body. We cannot discuss the relationship of many cells, because we do not know the relationship between cells through a single kind of cell. So cell co-culture medicine arises at the historic moment for the demand. With the development of research method in recent years, cell co-culture method also has been improved in practice: from direct contact co-cultures to indirect contact co-cultures, from two-dimensional co-cultures to three-dimensional co-cultures. Cell co-culture method is closer to the human body. It is also more advantageous to study the interaction among cells. Nowadays, there are more researchers tend to select this method to study the physiological and pathological in vitro model, tissue engineering, and cell differentiation research. At the same time, it has become the focus of drug research and development, drug analysis, mechanism of drug action, and drug targets. This article will review the studies of cell co-culture method, summarize advantages and disadvantages of various methods, so as to promote improvement of cell culture methods, to build cells co-culture system that more close to human body, and build the in vitro model that simulate internal circulation of human body further.

Objective To investigate the clinical significance of serum sialic acid (SA) in the diagnosis and treatment of IgA type multiple myeloma (MM). Methods The level of serum SA in 50 healthy subjects and 70 patients with MM were determined. Results In MM group, there were 25 cases of IgA type, 51 cases of other than IgA type (40 cases of IgG type, 7 cases of light chain type MM, 4 cases of non-secreting type MM). In healthy subjects, patients with IgA, IgG, light chain, non-secreting types and other than IgA type, the serum SA levels were (570.33 ± 67.72) mg/ L, (1289.24 ± 325.42) mg/ L, (585.88 ± 159.12) mg/L, (600.77 ± 126.90) mg/L, (590.50 ± 100.86) mg/L, and (588.39 ± 150.90) mg/L. The serum SA level of IgA type patients was higher than those of healthy subjects and other types (all P<0.01), and there was no significant difference among the other groups (all P> 0.05). The serum SA level of IgA type patients had positive correlations with serum IgA, globulin, bone marrow smear myeloma cell population and ESR (r values were 0.699, 0.753, 0.504, and 0.732, all P< 0.05); the serum SA level of IgA type patients had negative correlations with serum albumin, IgG, hemoglobin and platelet (r values were -0.712, -0.505, -0.629, and -0.596, all P< 0.05). The serum SA level of IgA type patients had no correlation with leukocyte, serum creatinine, serum IgM, lactic dehydrogenase, serum calcium, C-reactive protein and β2-microglobulin (all P>0.05). The level of serum SA in IgA type patients decreased with the improvement of the condition, and increased with the severity of the disease. Conclusions In MM patients, serum SA level in IgA type is high. The serum SA is an index for reflecting the tumor load capacity, and can evaluate the therapeutic efficiency of IgA type MM patients.

Objective To study the interphase FISH of cells from aminotic fluid in the third trimester prospectively. Methods Amniotic fluid (5ml) was obtained in 32 pregnant women in the third trimester who underwent cordocentesis for karyotyping. FISH was performed on uncultured am niocytes and the results were compared with karyotypes. Results Abnormal aneuploid were identified in 6 cases among the 32 women. The FISH results were consistent with the karyotypes. Conclusions Interphase FISH in third-trimester amniocentesis can provide an accurate clinical diagnosis on common aneupoidies.

Objective To evaluate the value of CT examination in localized diagnosis of primary mesenteric occupancy lesions.Methods CT findings in 35 cases of primary mesenteric occupancy lesions proved operatively and pathologically were analysed.Results Accuracy and postive forecast rate of localization of lesions by CT were 62.9% and 78.6% respectively.The findings of localized diagnosis included:(1)The relation between the occupancy lesions and intestines was closed,the situs was circled by intestines which were fixed and there was no filling defect in 25 cases.(2)The main retroperitoneal organs were distorted and their axes were rotated by the lesions in 15 cases.(3)There was fat space between the lesions and the retroperitoneal organs and the abdomen walls in 11 cases.(4)The main blood vessels of abdomen were compressed by the lesions or the lesions circled by vessels in 9 cases.The localized accuracy was lower when the lesions were larger.Conclusion CT is useful for localization of primary mesenteric occupancy lesions.CT combined with other imaging data and clinical symptoms can improve CT localized accuracy.