Objective The mitochondrial 16S rRNA gene PCR sequencing method was applied to identify the bird species involved in the case of bird remains.Methods Using frozen muscle tissue samples from 15 unknown bird remains,the PCR amplification of the 16S rRNA DNA barcode fragment was performed.Results Through sequence alignment and phylogenetic analysis,it was determined that the 15 samples were associated with six bird species,including four Streptopelia chinensis,one Turdus eunomus,five Passer montanus,two Chloris sinica,two Fringilla montifringilla,and one Phoenicurus auroreus.These species belong to 2 orders,6 families,and 6 genera,all of which are protected as listed species under the wildlife conservation regulations.Conclusion The 16S rRNA gene segment can be regarded as a reliable approach for accurately identifying bird species from remains,providing a dependable basis for qualitative and sentencing determinations in judicial cases.