Objective To investigate the clinical characteristic, surgical stage, operative approach, resection and stability reconstruction of dumbbell intra-extradural tumor of cervical spine. Methods From January 1999 to December 2005, 37 consecutive cases with cervical dumbbell intra-extradural tumor were retrospectively studied. 18 males and 19 females ranged from 18 to 80 years old were involved in this study, including 25 schwannomas, 3 neurofibromas, 5 multi-neurofibromas and 4 malignant schwannomas. According to tumor location and involved range, all tumors were divided into five stages: 8 cases in Ⅰ stage, 9 cases in Ⅱstage, 13 cases in Ⅲ stage, 5 cases in Ⅳ stage and 2 cases in Ⅴ stage. Resection and reconstruction were performed at 20 patients through posterior-lateral approach, 17 patients through anterior-lateral combined with posterior-lateral approach. Lateral mass screw internal fixation system were used in 26 cases, while anterior combined posterior fixation were performed in 5 cases and none fixation in 6 cases. Results The follow-up period was from 3 months to 7 years. 1 case developed a transient weakening of upper limb, 1 case developed anesthesia in posterior neck, and 1 case developed Horner's sign. Vertebral artery ligation was performed in 1 case because of vertebral artery injury. 2 cases with malignant schwannoma occurred local recurrence in 1-2 years postoperation and received second operation. The recent effects after operation were satisfactory in majority cases,with complete recovery of spinal cord function in 19 cases. 2 cases without fixation appeared recuration deformity in cervical spine in 1-2 years postoperation. Conclusion The surgical approach and operative methods must be selected according to the location, surgical staging, characters of tumors. Stability reconstruction plays important roles in maintaining stability of cervical spine. More care should be taken in procedure for protecting vertebral artery, cervical nerve and spinal cord.

Objective To explore the digital morphological and chromatic characteristics of erythrocytes infected with Plasmodium vivax. Methods The images of both normal and P. vivax infected erythrocytes were segmented and measured for morphologic parameters including area, length, breadth, perimeter, roundness, aspect ratio, equivalent circle diameter, as well as chromatic parameters including saturation and color (red, green, blue). A statistic analysis was performed for these parameters. Results Both morphological and chromatic parameters showed high significant differences between the normal and the infected erythrocytes, and high significant differences between the normal and the erythrocytes infected with different stages of P. vivax. Conclusion The differences mentioned above could be used as the basis for automatic identification of P.vivax in thin peripheral blood smears.

Objective To investigate the role of Beclin 1 in the genesis and development of osteosarcoma and the effect of Beclin 1 overexpression on the growth of the in vitro osteosarcoma cell line MG63.Methods Real time-polymerase chain reaction (RT-PCR) and Western blot were used to detect the expressions of Beclin 1 in MG63 and hFOB1.19 at mRNA and protein levels ; A eukaryotic clone of plasmid pEGFP/Beclinl fusion with protein EGFP/Beclin 1 was constructed and was transfected into human osteosarcoma cell line MG63 by using lipofectamine 2000.The effect of Beclin1 overexpressions on the proliferation of MG63 cells was evaluated by MTT assay.Cell apoptosis was measured by flow cytomerty(FCM).Results The mRNA and protein expression of Beclin1 in human osteosarcoma cell line MG63 was significantly lower than that in the human osteoblast cell line hFOB1.19(0.17 ±0.06 vs 0.43 ±0.11,t =29.493,P ＜0.01 ; 0.13 ±0.05 vs.0.25 ± 0.08,t =6.325,P ＜ 0.01).The transfection of pEGFP/Beclinl increased the mRNA levels of human osteosarcoma(5.34 ± 0.50) times in transfected tumor cells MG63.The rate of cell apoptosis was low in control or transfected with lipofectamine 2000 only cells at an average of(0.10 ± 0.05) ％.The apoptosis rate was significantly higher in pEGFP/Beclin1 transfected cells than control cells ((4.3 ± 0.8) ％,t =5.752,P ＜ 0.05).Conclusion Compared with control cells,Bedin1 is down-regulated in the human osteosarcoma cell line MG63,which indicate the role of Beclin 1 in regulating the malignant behaviors of osteosarcoma.Beclin1 overexpressions inhibits cell proliferation and induces apoptosis in MG63 cells.

Objective To investigate the effects of cinnamaldehyde and citral on DNA and RNA of Aspergillus flavus and A. fumigatus cells and their mechanisms. Methods A. flavus and A. fumigatus were incubated on Czapeks agar plate (treated with cinnamaldehyde and citral at different concentrations) at 26.5 ℃ for 3—6 d. The normal and treated cells were observed by laser scanning confocal microscope (LSCM) and image analysis to describe the DNA and RNA levels by quantity and localization. Results DNA and RNA levels were changed greatly and multinucleate coniospores appeared in the treated cells. Conclusion Cinnamaldehyde and citral have directly or indirectly interfered the conventional synthesis of fungal hereditary DNA and RNA and normal differentiation of conidiophore in A. flavus and A. fumigatus, thus inhibiting the normal cell cycle and the growth and propagation of fungi.

Objective:To study the influence of anti-tumor drug incorporation on the physicochemical properties of calcium phosphate cement(CPC).Methods: Methotrexate(MTX),epirubicin(EPI),hydroxy camptothecin(OH-CPT),and arsenic trioxide(As_2O_3) were incorporated,each in a proportion of 2%,5%,and 8%,into the powder-phase CPC.Untreated CPC was taken as control.The setting time,compression strength,and the microstructure of the resultant products were evaluated and tested.Results: Compared with control group,the setting time was significantly prolonged when 2% EPI was incorporated into CPC(P

0.2); whereas that in group Ⅲ 1.24 ?0.38g, significantly different (P0.05),the dietary energy re-striction did not affect the producing and scavenging of free radical in theorgans.

Objective To investigate the antitumor effect of special promoter-controlled Gibbon ape leukemia virus membrane fusion glycoprotein (GALV.fus) mediated by type Ⅰ herpes simplex virus (HSV-Ⅰ) on lung adenocarcinoma. Methods Recombinant HSV-Ⅰ plasmids encoding GALV.fus was introduced into green monkey kidney cells(Vero)by liposome to amplify the virus, and then the virus was transfected into lung adenocarcinoma (A549), human fetal fibroblasts (HFL-Ⅰ GNHu 5) and human lung adenocarcinoma xenografts which were established in nude mice subcutaneously to observe antitumor and cytotoxic effect in vitro and in vivo; Recombined cytomegalovirus (CMV) containing GALV.fus or enhanced green fluorescence protein were served as control. Results Recombinant HSV-Ⅰ virus were packed successfully. Heterotransplantative tumourigenicity of the tumour was 100% in nude mice after A549 cells were inoculated. Recombinant HSV-Ⅰvirus exerted obvious antitumor effects in vitro, with relative survival rate of 23%, while for CMV virus containing GALV. fus, the rate was 20%, and for CMV virus encoding EGFP, the rate was 68%. Recombinant HSV-Ⅰvirus also showed striking antitumor effect on the implanted tumor. Conclusion GALV.fus has powerful effect against lung cancer in vitro and in vivo and maybe a promising candidate for gene therapy.

Image analysis plays an important role in morphological study of tissues and cells and their quantitative analysis. It also contributes to clinical pathological diagnosis. With the rapid development of computer technology great progress has been made in the image analysis system, its measurement, rapidity, accuracy and the extent of automation have been greatly enhanced. In the meantime advances in medicine give impetus to its improvement. In this paper, the process of development, the basic structure and mechanism of image analysis system are discussed and the application of image analysis system to the study of biomedicinal is presented.
Image Processing, Computer-Assisted ; instrumentation ; methods ; Medicine

Objective To analyze the effect of compound Minwei on serum interleukin-4(IL-4),interferon-γ(IFN-γ)value in children with acute urticaria.Methods in our hospital from March 2015 to October 2016 received 148 cases of children with acute urticaria eczema,were randomly divided into study group and control group,74 cases in each group,study the application of compound chlorphenamine maleate and vitamin syrup for treatment,the control group were applied paeonol ointment treatment,curative effect,evaluation of children with symptoms before and after treatment respectively,and detection serum IL-4 and IFN-levels.Results before treatment,the two groups had no statistical significance with symptom score,serum IL-4,IFN-levels difference(P>0.05);after treatment,the clinical efficacy of the study group,serum IFN-γ levels higher than the control group,the serum level of IL-4 score,symptoms than the control group,the difference has statistical significance(P<0.05).Conclusion acute eczema urticaria children with compound Minwei syrup treatment,can significantly relieve the symptoms of children,improve the body immunity,promote children rehabilitation,has good treatment effect.

Objective To compare the dinical outcome of posterior Gallie method with cable fixation and Harms technique with C1,2 pedicle screw fixation in treatment of odontoid fractures. Methods From July 2003 to July 2008, 26 patients with types Ⅱ and Ⅲ odontoid fractures were treated surgically. There were 18 males and 8 femalses, at age range of 22-65 years ( average 43 years). The patients were divided into Gallie titanium cable fixation group (Gallie group, n = 14) and C1 -C2 posterior screw fixation group using Harms technique (Harms group, n = 12) according to treatment methods to compare blood loss, operation duration, costs, hospital stay, bone fusion time, complications and secondary operation. Results The patients were followed up for 18-84 months ( average 37.3 months). The average blood loss and costs in Gallie group were significantly less than that in Harms group (P<0.05), while the time for back to work in Gallie group was significantly longer than that in Harms group ( P < 0. 05 ). There was no statistical difference upon operation duration, hospital stay and bone fusion time in two groups (P > 0. 05 ). There was one patient with nonunion and two with delayed union in Gallie group and one with secondary operation due to implant failure in Gallie group, and one with secondary debridement due to wound infection and one with delayed wound healing in Harms group, with no statistical difference (P > 0. 05). Conclusions Both Gallie technique with titanium cable fixation and Harms C1,2 screw fixation have advantages of early walk and short hospital stay. Compared with the former, the latter technique costs more but can help early back to work.