Light environment is an important component of vision rehabilitation training room, and play a role for the people with visual disability. Based on the relationship between the low vision and light environment, the modification of vision rehabilitation training room were discussed in term of light environment, including light for different sorts and severities of visual disability, preventing glare, color rendering and stroboscopic effect and so on, and the objects to see. The possible schemes for modification were designed.

Total 7594 elderly subjects from three urban districts in Beijing were enrolled in the study with randomized cluster sampling design. Physical examination and fasting plasma glucose test were undertaken for all participants.Based on the diagnosis criteria from The Guidelines of Dyslipidemia Control for Chinese Adults in 2007,the prevalence of metabolic syndrome (MS) was 26.57％ (2018/7594) and the prevalence of central obesity was 42.04％ ( 3193/7594 ).Using waist circumference ( WC ) as independent variable and MS prevalence as dependant variable,we developed a “Central obesity-Metabolic Syndrome”multiple regression model.The results showed that among those having WC higher than cutoff point,with each 3.3 cm reduction of WC,the prevalence of MS decreased by 4.5％.Therefore,central obesity can be used an important clinical target of MS for both prevention and treatment purposes.

AIM： To estabish a HPLC assay for the determination of pirarubicin（Pir） in plasma.METHODS： Daunomycin（DM） was used as the internal standard.Plasma samples were extracted with CHCl3∶ CH3OH（2∶ 1） .0.4M NH4Cl buffer（pH=9.0） solution： CH2OH（1∶ 9） and the internal standard were added.Separation was carried out on a 250mm× 4.6mm（5μ m） phenomenex column with 0.04M KH2PO4（pH=3.0） ∶ CH3CN（68∶ 32） as mobile phase.Fluorescent detector was set at an excitation wavelength of 480nm and an emission wavelength of 550nm.RESULTS： The calibration curves for serum Pir was linear over the range of 5～1 000ng/ml（r=0.9 997） .The recovery of Pir was 95.3％ .The within-day and between-day variations were less than 5％ .T1/2β ， CLs， Vd and AUC of Pir were（12.8± 5.9） h， （128.3± 52.6） L/（m2· h） ， （1 754.3± 478.2） L/m2 and （428.7± 137.2） ng/（h· ml） ， respectively.CONCLUSION： The method is suited for monitoring blood concentration and pharmacokinetic study of Pir.

Objective To compare efficacy of indirect immunofluorescence (IFA) and real‐time fluorescent polymerase chain re‐action(PCR) in detection of mycoplasma pneumonia in children .Methods A total of 137 children clinically diagnosed as Mycoplas‐ma pneumoniae(MP) infection were selected and divided into groups by age ,including <1 years old group(35 cases) ,1- <5 years old group(69 cases) and 5-15 years old group(33 cases) .Blood specimen and throat swabs were collected and detected by using IFA and real‐time fluorescent PCR .At the same time ,all of the selected children were treated with conventional therapy ,according to total effective rate ,positive coincidence rates of the two methods were statistically analysed by age .Results The positive coinci‐dence rates in children with MP infection <1 years old and 1- <5 years old detected by using real‐time fluorescent PCR were high‐er than that detected by using IFA ,while among children 5-15 years old ,the positive coincidence rate was higher detected by using IFA compared with that detected by using real‐time fluorescent PCR ,all had statistically significant differences (P<0 .05) .The o‐verall positive coincidence rates of the two methods were not significantly different(P>0 .05) .Conclusion IFA and real‐time fluo‐rescent PCR both could be used as effective methods for detecting MP ,but there are some differences of detective efficacy between the two methods in each age group .Therefore ,it is suggested that for children under 5 years old real‐time fluorescent PCR might be selected ,for children aged 5 years old and over IFA might be selected ,in order to improve the detection accuracy and provide better guidance to clinical medication .

This article systematically introduced the development of Smart Healthcare mode in Hangzhou. From the aspects of connotation, pattern, characteristics and the change of medical treatment mode, the authors pointed out that the development of Smart Healthcare must follow such principles as satisfaction of the people, enhanced inter-departmental synergy, government-guided social participation, and full involvement of medical workers.

Background and purpose: The position of thymidine kinase 1 (TK1) expression during cell division is in the cytoplasm. It is a catalytic enzyme to convert deoxythymidine into thymidylate. It is the key enzyme of pyrimidine salvage pathway. The aim of this study was to analyze the serum expression level of TK1 in patients with breast cancer, and explore the application of serum TK1 test in clinical assessments of diagnosis, treatment and prognosis for breast cancer. Methods: Patient data were collected from the patients admitted in Comprehensive Breast Health Center at Rui Jin Hospital. Chemiluminesence dot blot assay was used to detect serum TK1 levels in 145 breast cancer patients and 55 patients with breast ifbroadenoma. The correlations of serum TK1 levels with breast tumor biological behavior was further studied. Results:Serum TK1 expression levels was signiifcantly increased in breast cancer patients [(2.749±0.122)pmol/L] when compared to breast fibroadenoma patients[(1.319±0.126)pmol/L, P<0.000 1]. Serum TK1 levels were statistically increased in patients with lymph node metastasis (P=0.049), distal metastasis (P=0.003 1), and late TNM stages (P=0.01). No serum TK1 level differences were found in patients with different ages (P>0.05), different tumor grades (P=0.453) and different tumor size (P=0.908). Preoperative imaging results including breast ultrasound, breast mammography and breast magnetic resonance were analyzed by assessments of BI-RADS category, and serum TK1 levels in patients with different BI-RADS categories were studied. Serum TK1 levels in patients with breast ultrasound BI-RADS categories 4C-6 were signiifcantly higher than those with category 0-4B (P<0.001). Consistently, the serum TK1 levels in patients with MR BI-RADS categories 4C-6 were higher than categories 0-4B (P=0.005). The serum TK1 levels in patients with mammography BI-RADS categories 4C-6 were higher than categories 0-4B (P=0.032). The serum TK1 levels were signiifcantly increased in patients with ER high expression in breast tumor tissues than those with low expression (P=0.034). Serum TK1 levels had no differences in patients with different expression levels of PR, HER-2 and MIB-1 (P>0.05). Most patients were followed up in our outpatient department for about 2 years. No progression-free survival differences were found in 2years. Conclusion:Serum TK1 test might be a potential tool for screening, prognosis determination and effect evaluations of targeted therapy in breast carcinoma.

Objective To evaluate the accuracy of ultrasonography and magnetic resonance imaging detection on preoperatively predicting axillary lymph node in breast cancer patients.Methods From August 2010 to February 2011,the clinical data of 293 breast cancer patients underwent preoperative breast ultrasound exam and MRI detection in Ruijin Hospital were analyzed retrospectively.Results In 293 breast cancer patients,3 suffered from bilateral breast cancer.The sensitivity,specificity,negative predictive value and positive predictive value of ultrasound were 60.0％,90.6％,78.0％ and 80.2％,respectively.The sensitivity,specificity,negative predictive value and positive predictive value of MRI test were 50.5％,90.8％,74.0％ and 77.9％,respectively.There was no significant difference between ultrasound and MRI test in these four indices (P ＞ 0.05).No significant difference on accuracy and predictive value was seen in subgroup analysis according to tumor size,tumor grade,hormone receptor and HER2 status between the two examinations.The sensitivity and specificity of ultrasound associated with MRI test were 70.6％ and 97.2％.Conclusions The accuracy and predictive value between preoperative ultrasound exam and MRI is comparable.Ultrasound associated with MRI examination can achieve high specificity,which may spare a portion of patients free from sentinel lymph nodes biopsy.

Objective To analyze the diagnostic methods of Achilles tendon rupture by MRI.Methods All 16 cases with Achilles tendon rupture were examined with sagital T 1,2 WI and axial T 1WI imaging.4 cases were appended Gd-DTPA enhancement.6 cases were followed up with MRI after 6～8 weeks.Results MRI appearance of Achilles tendon rupture represented Achilles tendon thickening,decrescent ratio of wide/vertical survey,hyperintensity in tendon,discontinuous fibrous bundle,fluid collected around tendon comparing with of normal Achilles tendon.Conclusion MRI is an valuable tool for the diagnosis of Achilles tendon rupture.

Objective To observe the effects of fennel essential oil and water extracts (distilled oil is not included) on gastrointestinal motility disorder caused by atropine in mice.Methods Kunming mice were randomly divided into blank control group, model group atropine, water extracts group, fennel essential oil group, mosapride group. Blank control group and model group atropine were orally administered with normal saline of 0.2 ml/10 g. Water extracts group was orally administered with Water extracts (75 mg/ml) of 0.2 ml/10 g. Fennel essential oil group was orally administered with Essential oil of 300 mg/kg. Mosapride group was orally administered with mosapride(15 mg/ml). Subjects were orally treated for 3 d. After fasting for 18 h, blank control group was intraperitoneally injected saline on the fourth day, and other groups were injected atropine sulfate injection to induce animal model of gastrointestinal motility disorder. Blue dextran(BD)2000 was used to observe the gastric emptying rate and rate of intestinal propulsion. Results Gastric emptying rates of fennel essential oil group, mosapride group, water extracts group and model group atropine were respectively(91.97±4.42)%, (90.26±5.81)%, (80.01±6.27)%,(72.88±9.13)%,and intestinal pushing rates were respectively(53.32±7.49)%,(53.02±9.13)%,(44.16±7.68)%,(37.52±6.19)%.Fennel essential oil, mosapride and water extracts enhanced the gastric emptying and intestinal propulsion in gastrointestinal motility disorder animal caused by atropine(P values were 0.004、0.001、0.004、0.003、0.025、0.015),where Fennel essential oil and mosapride were superior to the water,extracts(P values were 0.000、0.002、0.001、0.001).Conclusion Fennel extracts may promote gastrointestinal movement of atropine-induced gastrointestinal motility disorder in mice and fennel essential oil is the main active ingredient.

Aim To investigate the effect of Ajuga decumbens(HBG), Poria cocos(FL) and their combination on the metastasis of invasive breast cancer cells and the related mechanisms.Methods We conducted several assays including cell adhesion assay, scratch assay and transwell invasion assay.Western blot analysis was employed to detect the expression of associated proteins of EMT and MAPK signaling pathway.Results FL,HBG and their combination could significantly inhibit the adhesion, migration and invasion of MDA-MB-231 and SK-BR-3 cells.HBG and FL had markedly synergistic effect, and the best compatibility ratio was 10 ∶1.HBG, FL and their combination could reverse EMT of breast cancer cells, which increased the levels of epithelial biomarkers, such as β-catenin, E-cadherin and ZO-1, and reduced the levels of mesenchymal biomarkers, such as vimentin.Moreover, treatment of the cells with HBG, FL and their combination resulted in marked inhibition of phosphorylation of ERK1/2, JNK and p38.Conclusions The combination of HBG and FL have the ability to inhibit breast cancer cell invasion by targeting the expression of MAPK pathway as well as suppressing the epithelial-to-mesenchymal transition.