OBJECTIVE To investigate the present situation of hand-washing among medical staff then explore more suitable hand-washing methods and monitoring measure. METHODS By surveying the hand-washing situation of 96 medical staff in our hospital through the method of sampling,the different germ eradication rate between routine soap hand-washing and disinfectant wet towel hand-wiping as well as hand-washing rate in all candidate population during practical work were observed. RESULTS The germ eradication rate after routine soap hand-washing was 86.0%,while by disinfectant wet towel wiping it was increased to 91.3%.The washing rate after above former method was 18.3% and after above two methods combined was increased to 43.6%,and after health propaganding and training was up to 60.6%. CONCLUSIONS Disinfectant wet towel hand-wiping is convenient,practical and effective which would be more suitable for clinical practice when combined with routine soap washing method.

Objective To study the collision-induced dissociation fragmentation pathways of the jasminoidin and catalpol in ESI-Msn. Methods The samples were studied by using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-Msn) both in positive and negative. Results The collision-induced dissociation fragmentation pathways of both compounds were described. In the spectrum of two compounds, the loss of glucose residue and whole glucose were observed in positive mode, while only the loss of glucose residue could be observed in negative mode. Furthermore, we found that as the effect of the substituting groups, the fragment ions of jasminoidin were more in positive mode and those of catalpol were more in negative mode. Conclusion Conclusion The established method could be used for the sensitive and rapid identification of this kind of iridoid glycosides。

This report presented a case of 62-year-old woman who was admitted to our hospital for cirrosis, but she had a variety of clinical manifestations,such as abdominal distension,diarrhea,ascites, hepatosplenomegaly,anemia, palpitation, flushing, low blood pressure, arrhythmia and so on. Upper gastrointestinal endoscopy showed thicken mucosa at gastric fundus. Colonoscopy revealed nodular, pseudopolypoid. Ascitic fluid test suggested a transudate. Endoscopic abnormal mucosa biopsy showed chronic inflammation. Many mast cells were seen in bone marrow and liver biopsies,and liver tissue immunophenotype was CD117, and CD68. Thus the patient's diagnosis was systemic mastocytosis. It is rare that a patient only has the gastrointestinal tract symptoms complicated with ascites without skin lesion. The goal for treatment was to reduce hypersecretion of mast cells.

The clinical data of 186 patients with acute gout attack during upper gastrointestinal bleeding were analyzed retrospectively.The ratio of male to female was 1.7∶1, smokers and alcohol drinkers accounted for 66.1% ( 123/186 ) and 62.4% ( 116/186 ) of patients were had underlying diseases.All patients had different degree of joint painful and fever , the blood uric acid levels were higher.Gastroscopic examinations were performed in 166 patients , of whom 88 cases received endoscopic intervention.The symptoms of gout were improved after treatment with dexamethasone and celecoxib ; and the medication did not induce or increase gastrointestinal bleeding.

Objective To investigate the relationship between the nerve function and the sorbitol concentrations of erythrocyte and sciatic nerve, and to evaluate the effects and mechanism of a formulae of Chinese medicine, Shen Mai Huo Xue Yin (a decoction, it ingredients including ginseng, tuber of dwarf lilyturf and safflower) on diabetic peripheral neuropathy (DPN). Methods The rats were divided into 3 groups: normal rats, diabetic rats and diabetic rats treated with Shen Mai Huo Xue Yin. The diabetes of rats was induced by streptozotocin intraperitoneally. Motor nerve conduction velocity (MNCV) of sciatic, sensory nerve conduction velocity (SNCV) of caudal nerve and the H reflex were detected with evoked electromyogram; The sorbitol concentrations of erythrocyte and sciatic nerve was measured by spectrophotography. Results The sorbitol concentration of erythrocyte was positively correlated with that in the sciatic nerve, and the sorbitol concentration of erythocyte and sciatic nerve were negatively correlated with SNCV and positively with H reflex. The SNCV reduced significantly and the sorbitol concentration of both erythrocyte and sciatic nerve increased significantly in diabetic group as compared with those in normal group. The SNCV and sorbitol concentrations of sorbitol both erythrocyte and sciatic nerve in diabetic group were significantly improved after treated with Shen Mai Huo Xue Yin. Conclusion The sorbitol concentration in erythrocyte reflects the level of that in sciatic nerve and the lesion degrees of DPN. Shen Mai Huo Xue Yin alleviates the lesion of DPN in the early stage.

Objective To investigate the causes of death and the influencing factors in patients with peripheral arterial disease(PAD) during the perioperative period and reduce the perioperative mortality.Methods A retrospective analysis of the causes of death was performed for PAD during the perioperative period in our hospital from July 2005 to July 2010. Results Nine patients died preoperatively.The causes of death were multible organ failure in 5,heart failure in 2,sudden cardiac death in 1,and respiratory failure in 1.Six patients died postoperatively.The causes of death were acute renal failure in 2,heart failure in 2,cerebral infarction in 1,and hemorrhagic shock in 1.The causes of death which were related to heart,kidney and lung were in 10,6 and 2,respectively.The causes of death which were directly due to heart failure,sudden cardiac death,renal failure,respiratory failure,cerebral infarction and hemorrhagic shock were in 4,1,2,1,1 and 1,respectively.The main causes of death were multible organ failure,heart failure and acute renal failure,which accounted for 73.33％ of all deaths.In this series the overall perioperative mortality was 3.11％ (15/483),Operative mortality was 1.47％ (6/407).Conclusions The influencing factors and the function of organs were closely correlated with the causes of death. To reduce the perioperative mortality of PAD,it is necessary both to intervene in the influencing factors and to monitor and treat the dysfunction of vital organs.

In color flow imaging systems, the ultrasound Doppler signal scattered from blood is heavily corrupted by the strong clutter signal reflected from the slow-moving muscular tissue. Moving target indicator (MTI) is a key technique of rejecting the clutter signal and detecting blood flow. To improve the performance of detecting blood flow, a new MTI scheme is proposed in this paper. It consists of a pre-filter, a clutter weak-rejector and a 2-order AR estimator. After the processing of pre-filtering and clutter weak-rejection, the strong clutter signal is effectively eliminated and its strength is comparable with blood flow. Under such condition, the optimal performance can be attained by the 2-order AR estimator. It is illustrated in the simulation that, compared with the data from traditional methods, the blood flow parameters are estimated with smaller deviation and the lower speed flow is detected.
Blood Flow Velocity ; Equipment Design ; Humans ; Image Interpretation, Computer-Assisted ; instrumentation ; methods ; Ultrasonography, Doppler, Color ; instrumentation ; methods

Objective To retrospectively analyze medical data of patients with colorectal cancer (CRC) so as to provide evidence for clinical use of opportunistic screening.Methods A total of 2450 CRC patients (male 1377,female 1073) who were treated at five hospitals in North China during October 2001 and September 2011 and had complete medical records and pathological results were recruited.The correlations of incidenceofCRCwithage,gender,tumorlocationandhistologicaltypeswere analyzed.Results Of all the CRC patients,those less than 50 years old accounted for 18.14％ ; and the incidence of CRC was substantially increased in those over 50 years old.Seventy-three percent of tumor occurred at the rectum and sigmoid colon,6％ at descending colon,7％ at transverse colon and 14％ at ascending colon.Moderately,well or poorly differentiated adenocarcinoma accounted for 50.33％,40.35％and 9.32％,respectively.Histological differentiation was not correlated with age and gender ( P ＞ 0.05 ).Conclusions Age and gender should not be considered a determination of opportunistic screening for CRC.Colonoscopy is recommended as an alternative CRC screening procedure.

In order to test the performance of wall filters, we propose a method for simulation of Hybrid Doppler testing signal, which adopts a rectangular power spectrum. In the method, the parameters of clutter, blood flow and system noise can be adjusted with flexibility. The problem about choosing model parameters is discussed as an emphasis. Several reasonable and typical parameter combination patterns are presented. Furthermore, an example is given to explain the process of choosing the parameters and testing the wall filter performance.
Blood Flow Velocity ; physiology ; Computer Simulation ; Humans ; Rheology ; instrumentation ; methods ; Signal Processing, Computer-Assisted ; Ultrasonography, Doppler, Color ; instrumentation ; methods

This study was aimed to establish a method for the rapid content determination of Ixerin Z and 11,13α-dihydroixerin Z in Ku-Die-Zi (KDZ) injection by UPLC-ESI-MS/MS. The separation was performed on a Waters ACQUITY BEH C18 column (2.1 mm × 50 mm, 1.7μm) by using a gradient elution with the mobile phase of acetonitrile-water at the flow rate of 0.4 mL·min-1. The column temperature was set at 40℃. Multi-reaction moni-toring (MRM) scanning was employed for quantification in ESI negative mode. The results showed that two sesquit-erpene lactones in KDZ injection were totally separated within 2 min. The linear range of Ixerin Z was 5.70-182.50 ng·mL-1, and the linear range of 11,13α-dihydroixerin Z was 4.60-131.25 ng·mL-1. The correlation coefficient r was more than 0.999 0. The recovery rates (n = 6) were 98.18% and 97.52%, with RSDs < 1.5%. The established method was successfully applied for simultaneous content determination of Ixerin Z and 11,13α-dihydroixerin Z in 6 batches of KDZ injection from 2 factories, which had some variations on the content determination results. It was concluded that the method was rapid, accurate and sensitive, which can be used for the content determination of two sesquiterpene lactones in KDZ injection.