Objective To investigate the clinical effect of inhaled ribavirin on the therapeutic actions and the expression of interleukin 6 (IL -6),interleukin 10 (IL -10)and CysLTs in children with RSV bronchitis.Methods 81 cases of children with RSV bronchitis,were chosen and randomly divided into observing group and control group. Two groups of children were inhaled pulmicort respulas and accessed others support treatment,until the symptoms dis-appeared.But observing group were added with ribavirin atomization.Treatments time and symptoms disappearance time in the hospital were compared,and the serum IL -6,IL -10 and CysLTs level changes before and after treatment were observed.Results Treatments time and symptoms(wheezing,coughing,dry and wet rale)disappearance time in observation group were shorter than those of the control group with (6.58 ±1.36)d vs.(8.92 ±1.12)d,(1.21 ± 0.59)d vs.(2.68 ±1.22)d,(4.86 ±1.31)d vs.(7.34 ±1.39)d and (4.00 ±1.52)d vs.(6.37 ±1.58)d,which with significant differences (t =-8.387,-7.012,-8.267,-6.887,all P <0.01 ).Before the treatment,two groups had no significant difference (P >0.05 ).After treatment,the levels of serum IL -6 and CysLTs were significantly lower in the observation group,and the levels of IL -10 were higher with (76.59 ±10.77)ng/L vs. (116.81 ±10.81)ng/L,(34.40 ±7.19)ng/mL vs.(48.26 ±8.36)ng/mL and,(43.25 ±3.97)ng/L vs.(31.39 ± 4.81)ng/L.The significant differences were observed in two groups (t =-16.74,-8.024,12.315,all P <0.05). Conclusion Compared with general therapy,the curative effect was significant.Combined inhaled ribavirin and pul-micort respulas in RSV bronchitis early,can reduce patients'clinical symptoms and the treatment time.

Objective: To explore the effect of curcumin on proliferation and apoptosis of TK6 human diploid lymphoblastoid cells.Methods: MTT colorometric assay, 3H-TdR incorporation, cell cycle analysis were used to evaluate the effect on proliferation, and morphology microstructure. Flow cytometry was used to detect the effect on apoptosis induction on TK6 cell.Results: Curcumin could inhibit proliferation by blocking transition from G0/G1 to S phase, induce apoptosis in TK6 cells. The effective dose for proliferation inhibition and apoptosis induction is 20 ?mol/L.Conclusion: Apoptosis induction is one of important mechanisms for anti-mutagenic, anti-tumor action of curcumin.

Objective To investigate the clinical features,diagnosis and treatment of spontaneous rupture of renal angiomyolipoma (RAML). Methods A total of 23 cases of spontaneous rupture of renal angiomyolipoma were studied retrospectively.The main clinical manifestations were summarized as follows.Sudden and unexpected lumbar and abdominal pain occurred in 21 cases and lumbar swollen pain in 2.Of them 3 cases concomitantly had hematuria.Nausea and vomiting occurred in 2 cases,shock in 3 and fever in 1.All cases underwent B-ultrasonic examination and 21 had CT examination. Results Diagnosis of RAML was established preoperatively in 16 cases,but 4 cases were misdiagnosed for carcinoma of kidney,1 case for renal infectious disease.Two cases were diagnosed by frozen-section sample during operation.Nephrectomy was performed in 10 cases and nephron sparing nephrectomy in 12,and digital subtraction angiography (DSA) embolization in 1. Conclusions Spontaneously ruptured RAML often changes its clinical symptoms and imaging features,which may result in a high misdiagnosis rate and subsequent nephrectomy.Comprehensive analysis of clinical manifestations and image findings can improve preopreative diagnosis rate,and frozen slice sample examination during operation can avoid unnecessary nephrectomy.

Objective To investitgate the level of polychlorinated biphenyls in retail livestock meat.Methods Totally 28 samples of three kinds of meat including of beef,pork and mutton were collected during Aug-Dec.,2008.Twelve dioxin-like polychlorinated biphenyls(DL-PCBs) and six marker PCBs were extracted by Soxhlet extractor,cleaned up by FMS and qualitative and quantitative analyzedsis by isotope dilution high-resolution gas chromatography/high resolution mass spectrometry(HRGC/ HRMS).Results Levels of total 18 compounds including of 12 DL-PCBs and indicator PCBs in beef,pork and mutton were 1 607.60 pg/g fat,435.82 pg/ g fat,and 505.17 pg/g fat respectively,DL-PCBs concentration were 456.19 pg/g fat,46.37 pg/g fat and 77.50 pg/g fat respectively.Maker-PCBs concentration were 1 151.41 pg/g fat,389.45pg/g fat and 427.67 pg/g fat respectively.The levels of PCB toxicity equivalent(TEQ)(the median value)were:0.84 pg/g fat in beef,0.15 pg/g fat in pork,0.28 pg/g fat in mutton respectively.The TEQ concentration of PCB(median value) was lower than the EU action level limit value.In all samples,PCB118 was the greatest contributor to the total contaminated concentration,and PCB126 was the dominant contributor to toxicity equivalent(TEQ).Six of 28 samples exceeded the limit value of the EU action level.Conclusion The results show that PCBs contamination exists in a city from the six samples exceeding the EU standard limit.It should take measures to explore the cause of contamination of PCBs compounds and control the pollution in order to guarantee food safety effectively.

Objective To study the changes of plasma renin activity(PRA) and angiotensin Ⅱ(Ang Ⅱ) concentration in patients with acute cerebral infarction(ACI) as well as their clinical significance.Methods Radioimmunoassay was used to detect PRA and Ang Ⅱ concentration in 55 patients with ACI in the acute,convalescent phrase,and carried out correlative analysis with focal size and the degree of neurological deficit.Results (1) PRA and Ang Ⅱ in 3d of the acute phase of ACI were significantly higher than those in the controls ( P 3.0 cm 2) and the focus of middle infarct group(1.5 to 3.0 cm 2),but PRA and Ang Ⅱ in both groups were significantly higher than those in lacunar infarction group(

Objective To establish a method to measure Angiotensin Converting Enzyme activity in serum by MECC. Method Mixture of serum and substrate Hip-His-Leu had been incubated for 120 min at 37℃, substrate Hip-His-Leu was digested into two parts, Hip and His-Leu. The reaction was ended by 0. 1 mmol/L HC1 , the production was analyzed by MECC directly to detecte content of Hip in production, and calculated ACE activity. Running buffer was 20 mmol/L pH 9. 0 boric acid-borate buffer (including SDS 50 mmol/L) , capillary column was 75?m i. d.?37 cm, injection was 3s by press, voltage was 16 kV, running time was 7. 5 min, detected by UV detector at 200 nm, tempreture was 20℃. Result The within-run and between-run CV was 2. 7% and 5. 2%. The detection limits of ACE activity was 0. 2 IU/L ( singal/noise = 3). The ACE activity and absorption was linearly related from 2. 4 to 72 IU/L. The mean value of ACE was 5. 2-21. 9 IU/L (x?1. 96 s) in 50 normals. Conclusion It was a one of rapid, precise methods for determination of ACE activity in serum.

Objective To observe the curative effect of refractory ascites with ascitic ultrafiltration and reinfusion into abdominal cavity.Methods Thirty-seven patients diganosed as refractory ascites were given ultrafiltration and reinfusion into abdominal cavity.The following parameters were detected respectively:24h urine volume,abdomen circumference,heart rate,blood pressure,serum electrolytes,serum albumin,total serum bilirubin.Results After treatment,the 24h urine volume increased and abdomen circumference decreasd significantly (P ＜ 0.01 ).There was no significant difference in heart rate,blood pressure,serum electrolytes,serum albumin and total serum bilirubin before and after treatment (P ＞0.05).Conclusion Ascitic ultrafiltration and reinfusion into abdominal cavity has some effects on refractory ascites.

Objective:The clinical value of used in oral cavity repair were summarized to guide clinical practice. Methods:Retrospective analysis the clinical information of 144 cases(prefabricated posts:86 cases and metal posts:58 cases) who received oral cavity repair in our hospital between January 2010 and January 2012. Discuss the applications and clinical effects. Results:After 1 to 2 ears of follow-up, into a successful repair success rate of fiber post and metal post(93.02%v.s.79.31%, P=0.015), advance into a fiber post success rate is significantly higher than metal post, Repair the cause of the failure of the two groups, however, fall off and fracture was statistically difference(x2=5.51, P<0.019), the fiber pile of biomechanics is better than metal post, loss is the main reason for the advance into the fiber pile repair failure. Conclusions:As a new material in the oral cavity repair, prefabricated posts has been widely used and is worthy of further promotion.

Sexual transmission of HIV-1 has recently become the predominant route over the world.As an effective HIV vaccine has been elusive so far,microbicides,which are usually applied at topical mucosal sites to prevent vaginal or rectal mucosa from HIV-1 infection,have been paid ever increasing attentions.The failure of previous clinical trials have demonstrated that it is critical to ensure the safety and efficacy of microbicides before they move forward into clinical trials.In this review,we have summarized recent progress in preclinical models evaluating the safety and efficacy of microbicides,including cell culture in vitro,mucosal explant and animal models in vivo which are used at different phases during the preclinical development of microbicides,and have elaborated the characteristics of these models and their advantages.Finally,we emphasize the urgent need to establish effective evaluation systems at molecular levels for different models,which may serve as good surrogates to predict the inflammation and HIV infection in future clinical testing.