Objective To observe the effects of Huangqi injection associated with a combined treatment on changes of hemodynamics indexes and brain natriuretic peptide (BNP) after cardiopulmonary resuscitation (CPR) in rabbit models with cardiac arrest (CA) to elucidate the mechanism of such cardiac protective effects. Methods Twenty-four New Zealand rabbits were randomly divided into four groups(each,n=6). The CPR model in rabbits was reproduced by using extra-corporal electric shock method(put through 50 V alternating current to quiver on chest). The animals in operation control group were given anesthesia,all kinds of catheter inserted into the body and tracheotomy,but no induction of ventricular fibrillation. In the epinephrine group,epinephrine (30 μg/kg)was injected into the right jugular vein by a catheter during CPR. In the sub-hypothermia group, epinephrine(30μg/kg)was injected into the right jugular vein via a catheter and 0.9%sodium chloride under 4℃at a rate of 1.0 mL · kg-1 · min-1 was pumped into the ear marginal vein simultaneously with the chest external compressions during CPR and the cooling of body surface sustaining at the target temperature (32-34℃) for 4 hours until the end of the experiment. In the combined treatment group,Huangqi injection(4 g/kg)was injected into the right jugular vein on the basis of treatment of sub-hypothermia group. Hemodynamics indexes such as left ventricular end-diastolic pressure(LVEDP),the maximal rate of left ventricular pressure increase/decline(±dp/dt max),and the mean arterial pressure(MAP)were dynamically monitored at 15 minutes before inducing ventricular fibrillation and at the early stage of post resuscitation for 30,60,120,180,240 minutes,and in the mean time the concentration of BNP was dynamically monitored at 15 minutes before inducing ventricular fibrillation and at the early stage of post resuscitation for 30,180,240 minutes. Results Compared with those of the operation control group,the levels of LVEDP and BNP of epinephrine,sub-hypothermia and combined treatment groups were gradually increased obviously,while ±dp/dt max and MAP were decreased significantly after successful CPR. The levels of LVEDP and BNP at any time point in the combined treatment group were markedly less than those of the epinephrine and sub-hypothermia groups,and along with the time extension,the level of LVEDP had a tendency of elevation,and the level of BNP had a tendency of gradual decrease,reaching peak and valley values respectively at 240 minutes after CPR〔LVEDP (mmHg,1 mmHg=0.133 kPa):6.56±0.21 vs. 12.57±0.33,9.54±0.24,BNP(ng/L):199±19 vs. 286±14, 251±29,all P<0.01〕,at each time point,the ±dp/dt max and MAP levels were significantly higher than those in epinephrine and sub-hypothermia groups,and they were gradually decreased with the time prolongation,reaching valley values at 240 minutes after CPR〔+dp/dt max(mmHg/ms):4 229±353 vs. 2 055±311,3 224±158,-dp/dt max(mmHg/ms):3 587±168 vs. 1 315±189,2 357±245,MAP(mmHg):82.02±1.81 vs. 44.15±1.17, 56.79±1.60,all P<0.01〕. Conclusion Huangqi injection associated with a combined treatment has obvious protective effects on heart after CPR in rabbits,and the mechanism may be related to improvement of hemodynamics indexes and inhibition of excessive expression of BNP.

The cord dorsum potential evoked by stimulation in the ventrolateral aspect of periaqueductal grey (PAG-CDP) was recorded from the dorsal surface of the spinal lumbosacral cord in rats. PAG stimulation with the same parameters as for producing PAG-CDP inhibited the C discharges of convergent neurons in spinal dorsal horn. There was a positive correlation between the time course of the inhibitory effect of PAG on convergent neurons and the duration of the slow wave of PAG-CDP. a positive correlation also presented between the latencies of the PAG inhibition and the slow wave. Furthermore, the PAG inhibition did not appear when the intensity of PAG stimulation was below the threshold of PAG-CDP. These results indicate that the presynaptic inhibition is involved in the PAG inhibition of the C discharges of convergent neurons in spinal dorsal horn.

Objective To investigate the clinical features,diagnosis and treatment of spontaneous rupture of renal angiomyolipoma (RAML). Methods A total of 23 cases of spontaneous rupture of renal angiomyolipoma were studied retrospectively.The main clinical manifestations were summarized as follows.Sudden and unexpected lumbar and abdominal pain occurred in 21 cases and lumbar swollen pain in 2.Of them 3 cases concomitantly had hematuria.Nausea and vomiting occurred in 2 cases,shock in 3 and fever in 1.All cases underwent B-ultrasonic examination and 21 had CT examination. Results Diagnosis of RAML was established preoperatively in 16 cases,but 4 cases were misdiagnosed for carcinoma of kidney,1 case for renal infectious disease.Two cases were diagnosed by frozen-section sample during operation.Nephrectomy was performed in 10 cases and nephron sparing nephrectomy in 12,and digital subtraction angiography (DSA) embolization in 1. Conclusions Spontaneously ruptured RAML often changes its clinical symptoms and imaging features,which may result in a high misdiagnosis rate and subsequent nephrectomy.Comprehensive analysis of clinical manifestations and image findings can improve preopreative diagnosis rate,and frozen slice sample examination during operation can avoid unnecessary nephrectomy.

Objective·To explore plasma immune and inflammatory proteins that could serve as potential screening markers for Alzheimer's disease (AD).Methods·Healthy controls (n=19) and AD patients (n=19) were enrolled.Plasma samples were collected and 70 kinds of immune and inflammatory proteins were detected.The immune and inflammatory proteins associated with AD were screened by Mann-Whitney U test and partial correlation analysis.Discriminant analysis was used to develop multi-protein combined algorithm to distinguish plasma samples of AD patients from those of healthy controls.Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy for the multi-protein combined algorithm.Results·Among the 70 proteins analyzed,23 were significantly higher in AD patients (P＜0.05),among which 19 were strongly correlated with AD (P＜0.05).These 19 proteins were analyzed with Wilks' lambda stepwise analysis to develop discriminant algorithm for detecting plasma samples of AD.Finally,the discriminant algorithm established by 11 plasma immune and inflammatory proteins (EGF,GRO,MDC,MCP-1,MCP-2,MCP-4,TARC,SCF,TRAIL,CTACK,GCP-2) was found to have an optimal diagnostic efficacy (AUC=0.994).The optimal cutoff value of the algorithm was-0.609.When the optimal cutoff value was obtained,the sensitivity of the equation could reach 100％ and the specificity could reach 94.7％.Conclusion·The discriminant equation composed of the above 11 plasma immune and inflammatory proteins has the potential to assist AD screening.

Aim To explore the protective effects of dl-praeruptorin ( Pd-Ia) against acute lung injury induced by lipopolysaccharide ( LPS ) .Methods Acute lung injury model was induced by intranasal instillation LPS, and Pd-Ia was administered by intraperitoneal in-jection after 1 h of LPS exposure .Lung tissue samples were collected after 24 h of LPS administration to in-vestigate the role of Pd-Ia in acute lung injury .Results Pathomorpholoy showed a marked improvement of in-flammatory cell infiltration in Pd-Ia treated group , cel-lular staining also indicated a marked decrease of in-flammatory cells in BALF, and quantitative PCR and ELISA revealed a significant inhibition of cytokine IL-6,TNF-α, IL-1β, and chemokine MIP-1α, MIP-2 ex-pression .Pd-Ia attenuated LPS-induced histological se-verities and TNF-α, IL-6, IL-1β,MIP-1αand MIP-2 production .Conclusion Pd-Ia can alleviate the lung injury by ameliorating inflammation in lung .

AIM To explore the molecular mechanism of hydroquinone genotoxicity in human bronchial epithelial cells and investigate whether human X-ray repair cross complementing group 1 (XRCC1)was involved in protecting cells from the damage caused by hydroquinone. METHODS XRCC1 gene was knocked down by RNA interference and XRCC1-deficient cell was established by transfected recombinant plasmid pEGFP-C1-pU6-dsRNA. Normal human bronchial epithelial cells (normal cells) and cells transfected with the empty vector of pEGFP-C1 (vector cells) were used as the normal control and vector control. All cells were treated with different concentrations of hydroquinone (10-100 μmol·L-1) for 4 h. MTT assay was used to test cell viability and comet assay was used to detect the DNA damage and repairment. RESULTS MTT assay showed that hydroquinone inhibited the growth of cells in a concentration-dependant manner and the survival number of XRCC1-deficient cell was less than that of the two control groups. Comet assay revealed that different concentrations of hydroquinone caused more severe DNA damage in XRCC1-deficient cell line than in control cells and there were no significant difference in the two control groups. CONCLUSION The results suggest XRCC1 be involved in preventing cells from damage caused by hydroquinone.

Objective To investigate the immune response of the fusions of ovarian carcinoma cells to dendritic cell(DC) transfected with interleukin(IL)-12 gene in vitro. Methods Recombinant human granulocyte macrophage colony stimulating factor and recombinant human tumor necrosis factor ? were used to generate DC from cord blood in vitro. IL-12 fusion gene was cloned into a eukaryotic vector-pcDNA3.1(+). DC were transfected with IL-12-pcDNA3.1(+) using lipofectamine transfection method and electric transfection method respectively. Then polyethylene glycol mediated the fusion of transfected DC and ovarian carcinoma cells, and 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) test was carried out to observe the immune response. Results DC were generated in vitro from cord blood and expressed high levels of costimulatory(50%) and MHC Ⅱ molecules(99%). RT-PCR showed that IL-12-pcDNA3.1(+) had been successfully transfected into DC. RT-PCR and immunofluorescence analysis showed that DC were fused with ovarian carcinoma cells successfully. Then the fusion cells of ovarian carcinoma cells to transfected DC, and the fusions of ovarian carcinoma cells to DC were cocultured with peripheral blood mononuclear cell respectively. MTT test showed that both fusions could induce cytolytic T cell activity and lysis of ovarian carcinoma cells,and the former effect was stronger. Conclusion The cytolytic T cell activity induced by the fusions of ovarian carcinoma cells to transfected DC is enhanced.

Objective:To systematically evaluate the effect of serum albumin content on the prognosis of patients with severe traumatic brain injury (TBI).Methods:PubMed, Embase, Cohrane Library, China National Knowledge Infrastructure (CNKI), China Biomedical Literature Database (CBM), China Journals Full-text Database (CJFD), VIP database and Wanfang Database were used for retrieval from inception to November 10, 2020. Through retrieving Chinese and English literatures, relevant studies on serum albumin content in different types of TBI patients and in severe TBI patients with different prognostic results were included. The difference of serum albumin content in patinets with severe TBI with healthy people and patients with mild illness TBI, and the difference in serum albumin content between patients with good and poor prognosis of severe TBI were observed. At the same time, the differences in serum albumin content between subgroups were evaluated by subgroup analysis of the authors' country, literature quality and severity of the control group according to characteristics that might cause heterogeneity.Results:A total of 9 articles were included in this study, including 1, 438 study subjects. The serum albumin content in patients with severe TBI was significantly lower than that in healthy individuals and patients with mild TBI ( WMD=-6.42, 95% CI -9.95--2.80, Z=3.82, P<0.01). Subgroup analysis showed that serum albumin content in patients with severe TBI was significantly lower than that in healthy people ( WMD=-8.77, 95% CI-10.28--7.25, Z=11.37, P<0.01). Serum albumin content in patients with severe TBI was significantly lower than that in patients with mild TBI ( WMD=-4.07, 95% CI -9.53 -1.39, Z=1.46, P<0.05), suggesting that mild TBI patients are the main source of heterogeneity. The serum albumin content in patients with poor prognosis of severe TBI was significantly lower than that in patients with good prognosis of severe TBI ( WMD=-4.48, 95% CI-7.39--1.58, Z=3.02, P<0.01). Conclusion:Serum albumin content in patients with severe TBI is significantly lower than that in healthy individuals as well as in patients with mild TBI, and low albumin content indicates a poor prognosis in patients with severe TBI.

OBJECTIVETo explore the effects and the molecular mechanisms of 4-phenylbutyric acid (PBA) on carbon tetraehloride (CCl4)-induced acute liver injury in mice.

METHODSSixty adult, healthy, male ICR mice were divided equally into the control group, PBA group, CCl4 12 h group, CCl4 24 h group, CCl4 48 h group, CCl4 72 h group, PBA+CCl4 12 h group, PBA+CCl4 24 h group, PBA+CCl4 48 h group, and PBA+CCl4 72 h group. The CCl4 groups and the PBA+CCl4 groups were intraperitoneally (i.p.) injected with CCl4 (300 mL/kg). In the PBA+CCl4 groups, the mice were i.p. injected with PBA (400 mg/kg). All mice were sacrificed to collect blood and liver specimens at different time points after the CCl4 treatment. Serum alanine aminotransferase (ALT) was detected. Histological examination was performed using hematoxylin-eosin staining and light microscopy, and apoptosis was detected using terminal transferase dUTP nick end labeling. The hepatic distribution of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. The hepatic protein expression of glucose-regulated protein (GRP78), C/EBP homologousprotein (CHOP), phosphorylated c-Jun N-terminal kinases (p-JNK), phosphorylated eukaryotic initiation factor 2a subunit (p-eIF2a), phosphorylated serine threonine kinase (p-akt), and nuclear factor-kappa B p65 (NF-kappa Bp65) were determined by western blot.

RESULTSThe serum ALT level in the PBA+CCl4 groups was reduced as compared with that in the CCl4 groups at the various time points examined.The liver-to-body weight ratio of two groups showed a significant difference only at the 48 h time point (P<0.01). PBA reduced the degree of hepatic necrosis and apoptosis caused by CCl4, and reduced the expression of hepatic GRP78 and other endoplasmic reticulum stress-related proteins (P<0.01). The protein levels of p-akt, NF-kappa Bp65 and PCNA was significantly decreased in the PBA+CCl4 groups (P<0.01).

CONCLUSIONThe endoplasmic reticulum stress inhibitor PBA alleviated acute hepatic necrosis and apoptosis but restrained hepatic proliferation.

Alanine Transaminase ; Animals ; Apoptosis ; Carbon Tetrachloride ; Chemical and Drug Induced Liver Injury ; Endoplasmic Reticulum Stress ; HSP70 Heat-Shock Proteins ; Male ; Membrane Proteins ; Mice ; Mice, Inbred ICR ; NF-kappa B ; Phenylbutyrates ; Phosphorylation

Objective To investigate the dynamic images of traumatic penumbra(TP)in a rat model of traumatic brain injury (TBI)treated with AQP4-RNAi using multimodal MRI(MM-MRI)at 7.0 Tesla.Methods A rat model of TBI was established by the improved Feeney's method.MRI scans were performed including T2WI,DWI,ADC and SWI.The pathological changes in penumbra area were observed.All statistical analyses were performed using SPSS 21.0 software package.Results Over time rs-T2WI,rs-DWI and rs-SWI in TBI group were gradually increased.The r-ADC began to increase at one hour after trauma and reached the peak at 6 h. Then it began to fall and reached the bottom at 12 h.At each time point,the difference was statistically significant(P<0.05).Compared with RNA interference group,the rs-T2WI and rs-DWI in TBI group were decreased significantly at 6 h and 12 h(P<0.05),the r-ADC was not decreased significantly at 1 h(P>0.05)and decreased significantly at 6 h and 12 h(P<0.05).At each time point,there was no significant difference in rs-SWI(P>0.05).The area of mismatch between rs-SWI and rs-DWI was the most obvious at 6 h and 12 h,and the area of mismatch was decreased in size after treatment with AQP4-RNAi.There was no significant difference between TBI group and placebo group(P>0.05).In two groups,similar pathological changes were observed,which was depicted as vasogenic edema predominantly at 1 h and mixed edema at 6 h and 12 h. In RNA interference group,intracellular edema was markedly reduced at 6 h and 12 h,and the vasogenic edema was relieved to some extent at 12 h.Conclusion The treatment with APQ4-RNAi markedly alleviates cerebral edema.MM-MRI can reflect its pathological changes.The area of mismatch between SWI and DWI can prompt early detection of traumatic penumbra,which may provide useful information for clinical treatment.