Objective To establish the quality standard for Xiaoyao Effervescent Tablets.Methods Radix Paeoniae Alba,Radix Angelicae Sinensis,Radix Bupleuri and Radix Glycyrrhizae in the tables were identified by TLC.Paeoniflorin content was determined by HPLC.Results Radix Paeoniae Alba,Radix Angelicae Sinensis,Radix Bupleuri and Radix Glycyrrhizae could be identified by TLC,and the identification was highly specific without interference.The linear range of paeoniflorin was 0.082 ? g～ 1.025 ? g.The average recovery was 100.44 %(RSD=2.37 %).Conclusion The method is simple,reliable,and accurate,and can be used for the quality control of Xiaoyao Effervescent TabLets.

Objective To investigate the expression of serum Golgi protein 73 in hepatocellular carcinoma(HCC) and analyze the clinical significance.Methods The expression of GP73 was measured by ELISA in 75 HCC ,30 chronic hepatitis and normal con -trols.Results The serum concentrations of GP73 were (128.3 ±33.6)μg/L,(80.3 ±19.2)μg/L and (78.3 ±18.5)μg/L in the HCC, chronic hepatitis patients and normal controls .The serum level of GP73 was significantly higher in HCC than those with chronic hepatitis and healthy controls .GP73 expression was positively correlated with clinical stage , humor size and metastasis.The positive rate of GP73 in stage was 60%,higher than the AFP positive rate(33%).Conclusions The serum level of GP73 is high in HCC and was helpful for distinguishing benign and malignant liver diseases .GP73 can be used as a diagnostic marker for HCC .

The paper analyzes the current status and existing problems of citation retrieval work, puts forward the design principle, overall structure and function of cited references integration tools, demonstrates the key technologies of system realization.An integrated retrieval platform for online literature citations is constructed for the following functions:one-stop retrieval of various citation databases, automatic removal of duplications, so as to reduce the burden of staffs and improve efficiency effectively.

Objective To investigate the influence of two different dose of atorvastatin on the prognosis and endothelial function in post-PCI acute coronary syndrom patients.Methods 92 post-PCI ACS patients were randomly divided into two groups,atorvastatin 20mg and atorvastatin 10 mg group.In each group the patients were treated with atorvasta- tin 20mg or 10mg respectively.After one month we add or decrease the dose of atorvastatin according to the blood lipid level.After 12 month the blood lipid level、FMD、NO、ET、NOS、UAP、AMI were compared between two groups. Results The clinical setting have no significant association between two groups before treating,After treated 1 and 12 month the TC,LDL-C level were significantly decreased as compared with the base level before treating in each group. After treated 1 month,in atorvastatin 20 mg group the TC,LDL-C level were significantly decreased and NO、NO/ET level were significantly higher than those in atorvastatin 10 mg group.During 12 month follow up the incidence of angina pectoris onset and rehospitalization were significantly higher in atorvastatin 10 mg group(P

Objective To explore the influencing factors of fear of progression among postoperative elderly patients with prostatic cancer. Methods From December 2016 to December 2017, we selected 404 elderly prostatic cancer patients with surgical treatment of Urinary Surgery in Tangshan People's Hospital as subjects by purposive sampling. All of the patients were investigated with the Fear of Progression Questionnaire-Short Form (FoPQ-SF), Readiness for Hospital Discharge (RHD), the Chinese Version of the M. D. Anderson Symptom Inventory and Family Adaptability and Cohesion Scale Ⅱ (FACES Ⅱ). Binary Logistic regression was used to analyze the influencing factors of fear of progression among postoperative elderly patients with prostatic cancer. Results The total of 404 elderly prostatic cancer patients were divided into two groups according to FoPQ-SF, 202 patients with the score of FoPQ-SF less than or equal to 34 in control group and 202 patients with the score of FoPQ-SF more than 34 in case group. There were statistical differences in ages, family support, clinical stages, scores of Gleason, symptom distress and readiness for hospital discharge of patients between two groups (χ2=10.164, 24.762, 21.083, 21.052, 21.915, 25.783;P＜ 0.05). Logistic regression analysis showed that the influencing factors of fear of progression among postoperative elderly patients with prostatic cancer included ages, family support, readiness for hospital discharge, symptom distress and scores of Gleason (P＜ 0.05). Conclusions Fear of progression among postoperative elderly patients with prostatic cancer is affected by many factors. Improving patients' readiness for hospital discharge will help to lower the fear of progression of patients.

BACKGROUND:Bone marrow stem cell transplantation can improve heart function and prevent ventricle remodeling.At present,the adult bone marrow stem cells used for transplantation primarily included bone marrow mononuclear cells (BM-MNCs) and mesenchymal stem cells (MSCs),and endothelial progenitor cells.The curative effects and precise mechanisms of transplantation of various bone marrow stem cells remain unknown.OBJECTIVE:To compare the effects of transplantation of autologous BM-MNCs and MSCs via the coronary artery on ventricle remodeling subsequent to acute myocardial infarction (AMI). DESIGN,TIME AND SETTING:Randomized controlled animal experiment performed at the Center for Clinical Research,Hebei Provincial People's Hospital,Electron Microscope Room,Hebei Medical University between March 2005 and December 2006.MATERIALS:Thirty-six male Jizhong pigs,were randomly divided into 4 groups:control group (n = 6),infarct model group (n = 10),BM-MNC group (n = 10),and MSC group (n = 10).METHODS:Porcine autologous BM-MNCs were isolated by gradient density centrifugation,and MSCs were obtained by adherence method.Prior to transplantation,both BM-MNCs and MSCs were colloidal gold labeled.Except the infract model group,pigs in the other 3 groups were developed into AMI models by oppressing the left anterior descending branch with balloon catheter.Ninety minutes after modeling,(6.0±1.3)×107 autologous BM-MNCs and (4.5±2.1)x 107 MSCs were respectively transplanted into pigs in the BM-MNC group and the MSC group via the coronary artery and cultured for 28 days.MAIN OUTCOME MEASURES:Observation of pathological changes of cardiac muscle tissue by light and electron microscope;Examination of cardiac function by ultrasonograph;Detection of the number of blood vessels and apoptotic myocardial cells,and expression of nuclear factor-κB (NF-κB) and troponin Ⅰ and its correlation to cardiac function by immunohistochemistry;Detection of mRNA expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the cardiac tissue as well as its correlation to cardiac function by reverse transcription-polymerase chain reaction (RT-PCR).RESULTS:In the MSC group,there was proliferation of a great deal of blood vessels as well as growth of abnormal cell masses around the coronary vessels,while the BM-MNC group exhibited the "budding" of many capillary vessels.Prior to transplantation,cardiac function indices were basically similar among each group (F = 1.550,P＞0.05).Twenty-eight days after transplantation,left ventricular ejection fraction was significantly lower in the control,BM-MNC,and MSC groups than in the infarct model group (F = 5.30,P＜0.05),while endocardial fractional shortening was significantly higher (F = 10.67,P＜0.01).Compared with the infarct model group,the number of blood vessels in the infarct zone and infarct border zone was increased in the BM-MNC group (F=29.56-34.87,P＜0.01) and had no apparent change in the MSC group.In the BM-MNC and MSC groups,apoptotic myocardial cells in the infarct zone and infarct border zone were significantly reduced (F=14.31-35.34,P＜0.01 ) and troponin I expression rate was significantly increased (F=19.05,P＜0.01 ),as compared with the infarct model group.In addition,NF-κB positive rate in the infarct border zone was significantly lower in the BM-MNC and MSC groups than in the infarct model group (F=19.05,P＜0.01).VEGF gene expression level in the infarct border zone was significandy higher in the BM-MNC group than in the infarct model group and MSC group (F = 49.41,P＜0.01).bFGF gene expression level in the infarct border zone was significantly higher in the MSC group than in the infarct model and BM-MNC groups (F=4.71,P＜0.01).LVEF was negatively correlated to myocardial cell apoptosis rate and NF-κB level (r=-0.441 1,P＜0.05;r=-0.579 6,P＜0.01 ).LVEF was positively correlated to number of blood vessels,VEGF and bFGF expression (r=0.775,P＜0.01;r=0.565 1,P＜0.05;r=0.573 5,P＜0.05).CONCLUSION:Transplantation of both autologous BM-MNC and MSC via coronary artery can improve the condition of left ventricular remodeling subsequent to myocardial infarction.The improvement of cardiac functions is related to the increase of blood vessels,VEGF and bFGF expression,the decrease of myocardial cell apoptosis and NF-κ B level in cardiac muscle tissues after stem cell transplantation.BM-MNC transplantation better promotes blood vessel proliferation and VEGF expression in the cardiac tissue but produces worse effects on bFGF gene expression than MSC transplantation.

The correlation of single nucleotide polymorphism (SNP) rs10569304 on the second expressed region of hole gene and congenital heart disease (CHD) of human being, and the effect of hole gene on CHD were investigated. 179 patients with CHD as CHD group and 183 healthy people as control group were selected in the case-control study. DNA was abstracted from the peripheral blood by phenol-chloroform method. Primer was designed for the flanking sequence of SNP rs10569304 on the second expressed region of hole gene. The genotype was identified by PCR degenerative acrylamide electrophoresis with amplification products. Then the three amplification products received sequencing. By chi-square test, the genotype frequency and allele frequency in CHD group and control group were analyzed. There was insertion-deletion (GCC/-) of SNP rs10569304 which corresponded to alleles of A and B in Southern Chinese people. The genotype frequency and allele frequency in control group and CHD group were met the Hardy-Weinberg equilibrium. By chi-square test, in control group and CHD group, the genotype frequency of AA (insertion homozygous), AB (insertion-deletion heterozygous) and BB (deletion homozygous) was 21.31%, 54.09%, 24.59% and 16.75%, 46.36%, 36.87%, respectively. The distributional difference of genotype frequency had statistical significance (chi (2)=6.51, P<0.05); The allele frequency of A and B was 48.36% and 51.64% in control group, 39.94% and 60.06% in CHD group, respectively. The distributional difference of allele frequency had statistical significance (chi (2)=5.20, P<0.05). Meanwhile, by contrast with the control group, the BB genotype frequency and B allele frequency in CHD group was higher, but the AA and AB frequency was lower. There was higher risk to suffer from CHD involving B allele. BB genotype had 1.907-fold increased risk of developing CHD according to AA genotype (P<0.05). It is concluded that there is insertion-deletion (GCC/-) of SNP rs10569304 in the Southern Chinese people, and the people whose hole gene involving BB genotype have higher risk to suffering from CHD.

After an overall introduction of the novel functions of the CMCI institutional knowledge version developed by Medical Library of Chinese PLA , its coverage of institutions, authors, funds, journals and literature was de-scribed in terms of RDE semantics.The contents in literature were mined and analyzed in depth based on the litera-ture-marked information with the institutions database, funds database, journals database and literature database as their cores, the association of information resources in biomedical field was thus realized.The CMCI institutional knowledge version can be used to search published papers and citations, write citation reports, enforce bibliometric visualization analysis, issue different top-ranked statistical data, push associated information, increase institutional literature management modules, and provide personal literature management and assessment service for different in-stitutions.

Objective To detect the changes of 25-hydroxy vitamin D (25-OH-D) and blood calcium concentrations in the serum of prostate cancer (Pca) patients with bone metastases,and to analyze the correlation between bone calcium metabolism and bone metastasis of prostate cancer.Methods The 35 untreated prostate cancer patients without bone metastases,30 patients with bone metastasis of prostate cancer,and 30 healthy controls were selected,and fluorescent immune chromatography was used to determine the content of 25-OH-D,the Azo arsenic method was used to detect the blood calcium level,and ROC curve was used to analyze the value of two indexes in diagnosing prostate cancer bone metastases.Results The blood calcium content in bone metastasis of prostate cancer group was significantly higher than the other groups (P ＜ 0.05),and the prostate cancer group had a higher blood calcium content than healthy controls (P ＜ 0.05).For 25-OH-D content,bone metastases of prostate cancer group was significantly lower than the other groups (P ＜0.05).25-OH-D level showed a negative correlation with blood calcium in each group (r =0.628,P ＜ 0.05).The ROC curve showed levels of blood calcium and 25-OH-D in the diagnosis of bone metastases of prostate cancer were 0.763 and 0.712.Conclusion The high blood calcium and low vitamin D levels may be an effective predictor for bone metastases of prostate cancer.

Objective To detect the changes of 25-hydroxy vitamin D (25-OH-D) and blood calcium concentrations in the serum of prostate cancer (Pca) patients with bone metastases,and to analyze the correlation between bone calcium metabolism and bone metastasis of prostate cancer.Methods The 35 untreated prostate cancer patients without bone metastases,30 patients with bone metastasis of prostate cancer,and 30 healthy controls were selected,and fluorescent immune chromatography was used to determine the content of 25-OH-D,the Azo arsenic method was used to detect the blood calcium level,and ROC curve was used to analyze the value of two indexes in diagnosing prostate cancer bone metastases.Results The blood calcium content in bone metastasis of prostate cancer group was significantly higher than the other groups (P ＜ 0.05),and the prostate cancer group had a higher blood calcium content than healthy controls (P ＜ 0.05).For 25-OH-D content,bone metastases of prostate cancer group was significantly lower than the other groups (P ＜0.05).25-OH-D level showed a negative correlation with blood calcium in each group (r =0.628,P ＜ 0.05).The ROC curve showed levels of blood calcium and 25-OH-D in the diagnosis of bone metastases of prostate cancer were 0.763 and 0.712.Conclusion The high blood calcium and low vitamin D levels may be an effective predictor for bone metastases of prostate cancer.