The pathogenesis of post- infectious irritable bowel syndrome(PI-IBS)involves abnormal intestinal immune barrier, which possibly includes the pathological aspects: Intestinal and systemic changes in T lymphocyte; the change of inflammatory cytokines; a increase in intestinal mast cells and enterochromaffin cells and so on. Treatment by western medicine based largely on symptomatic treatment. TCM thinks that the fundamental organ of the irritable bowel syndrome is the liver. Emotional factors inducing liver dysfunction lead to the symptoms of irritable bowel syndrome. The pathogenesis of IBS is, the disorder of movement of qi in liver and spleen. Stagnation of liver-QI with deficiency of the spleen or disharmony between liver and spleen is the key pathogenesis of post-infectious irritable bowel syndrome. On this basis, in various stages of the disease, there may be a different pathogenesis. As a special type of IBS, the balance of vital qi and evil factors determine the disease progression of PI-IBS, in the treatment course also should not forget dispelling pathogenic factors while strengthening body resistance, tonifying deficiency and removing dampness。

BACKGROUND: High blood lipid can cause a series of cardiovascular and cerebrovascular disease, which has been commonly accepted.OBJECTIVE: To probe into the rule of changes of blood lipid of the elders in aging process.DESIGN: Cross section survey.SETTING: Department of Nutrition, Shanghai Huadong Hospital. PARTICIPANTS: Totally 605 employees who took part in body examination and participated this experiment voluntarily from July 2000 to August 2004, were recruited, including 541 males and 64 females, 91 patients ＜ 70 years , 514 ≥ 70 years, 271 patients having the history of coronary heart disease, 83 of malignant tumors, and 251 of other diseases.METHODS: Blood lipid level of totally 605 who accepted physical examination was detected, at the same times, the disease history and common physical examination status were inquired. Venous blood of the patients was drawn after fasting over 12 hours, separating serum on the morning of the same day. Total cholesterol and triacylglycerol of the blood were detected with enzyme assay by using Automated Biochemistry Analyzer. Total cholesterol: 3.6-6.5 mmol/L; triglyceride: 0.4-1.8 mmol/L were considered as normal level, Total cholesterol: ＞ 5.72 mmol/L;Triglyceride: ＞1.72 mmol/L were considered as abnormal criteria.MAIN OUTCOME MEASURES: Comparison of the blood lipid index of the subjects of different age, disease type and gender RESULTS: Totally 605 elders were involved and all data of them was enthe blood lipid index of the subjects of different age: Peak of total cholesterol appeared at 70 to 74 years, then decreases gradually, the total cholesterol level of the subjects of 70 to 74 years was significantly higher than that at other age (P ＜ 0.05). While Triglyceride level had the trend of going up with growing older, except that of 75-79 ages group slightly went eases: The level of total cholesterol and triglyceride of the subjects in the malignant tumor group was lower than that of other disease groups, level of triglyceride of the subjects in the coronary heart disease was higher than detection rate of blood lipid of the subjects: Detection rate of total cholesterol was 15.5%, while that of triglyceride was 35.9%. There was no significant difference of the detection rate between male and female subjects (P ＞ 0.05).CONCLUSION: Change of blood lipid metabolism of elder with aging is related to the hormone change of different genders and disease types.

Objective To know the point prevalence rate of social anxiety disorder(SAD) in Daqing University,and explore the influence factors about growth environment.Methods Data came from a large representative sample of 1878 university students,who were selected by stratified random cluster sampling methods according to the constituent ratio of students.They finished general situation questionnaire,interaction anxiety scale (IAS),adolescent self-rating life events check list(ASLEC),Egma Minnen av Bardndosnauppforstran(EMBU) respectively.According to the score of IAS,they were divided into 3 groups of high,mid and low.And then all of the high group,the 50％ and 10％ randomly of the mid and the low group respectively accepted the face-to-face interviews with 6 trained psychiatrists referred to SCID-I/P.Results Among 1878 Daqing university students,191 of them were diagnosed as SAD,with a point prevalence rate of 8.89％.The rate of rural students was higher than that in urban students(12.3％ vs.8.5％,x2 =7.966,P=0.017),and the rate of non-only child was higher than only child (12.0％ vs.9.1％,x2 =4.055,P=0.044).There were significant differences between SAD and No-SAD on many factors of ASLEC and EMBU.Logistic regression analysis showed that study stress in ASLEC(B=0.206),father's warm (B =-0.070),father's overprotection (B =0.286),mother's punishment (B =0.159),only child or not (B =-0.685) entered into regression equation.Conclusion The point prevalence rate of SAD in Daqing university students is 8.89％ and influenced by several growth environmental factors.Only child or not,father's warm,father's overprotection,mother's punishment,study stress in ASLEC can forecast SAD occurrence.

Objective:To explore the relationship between social anxiety and sex role in college students,and to analyze the mediating effect of self-acceptance between them.Methods:Data came from a sample of 2000 col-lege students in Daqing,who were selected by stratified random cluster sampling method according to the constitu-ent ratio of the college students.We used the Interaction Anxiety Scale (IAS)to assess subjective experience of so-cial anxiety,Bem Sex-Role Inventory (BSRI)to evaluate sex-role classification and Self-Acceptance Questionnaire (SAQ)to measure characteristics of self-acceptance.Results:In the college,the distribution of sex-role classifica-tion were androgynous 656 (34.9%),undifferentiated 650 (34.6%),masculine 287 (15.3%),feminine 284 (15.1%).The differences of gender in scores of IAS and SAQ were not significant (Ps >0.05),but there were significant differences among sex roles (P <0.001).IAS scores of feminine were significantly higher than the oth-ers,and the scores of androgyny and masculine were both lower than the other two significantly.SAQ scores offeminine and undifferentiated were both significantly lower than the other two respectively (Ps <0.05).By Boot-strap test,masculine,undifferentiated and feminine (vs.androgynous)could mediate IAS through self-acceptance, the mediating effects were 0.76 (95%CI:0.09 -1.42),4.14 (95%CI:3.43 -4.93),and 4.03 (95%CI:3.47 -4.64) respectively.There was a mediating effect.Both undifferentiated (vs.androgynous ) and feminine (vs.androgynous)directly influenced IAS significantly (Ps <0.05).Conclusion:Social anxiety may be related to sex role but not gender,social anxiety level of feminine students is higher,the sex-role types could influence social anxiety through self-concept indirectly.

Objective:To investigate the effects of slowly release(SR) carbohydrate system on glucose and lipide metabolism in the elder patients with type 2 diabetes. Methods:The previous enteral nutrition was replaced totally or partially with Glucerna in 11 cases of elder patients with type 2 diabetes for 1-3 months.The changes in hemoglobin, lymphocyte counts, serum albumin, total protein, glycemia, postprandial 2 hours blood glucose,glycosylated hemoglobin, triglyceride, cholesterol, kidney function,electrolytes and the symptoms of gastrointestinal tract were observed. Result:Total protein(P

AIM: To examine the effect of cholecystokinin-octapeptide (CCK-8) on focal cerebral ischemia/reperfusion injury and its underlying mechanisms. METHODS: By using the suture model of focal cerebral ischemia and reperfusion, the effects of intracerebroventricular (icv) injection of CCK-8 and proglumide, nonselective CCK receptors antagonist, on the infarct size, regional cerebral blood flow (rCBF), and the levels of nitric oxide (NO), malondialdehyde (MDA) were observed in different brain regions of rats subjected to 1 h focal cerebral ischemia followed by 24 h reperfusion. RESULTS: (1) pretreatment with different doses of CCK-8 (0.3 ?g,1.0 ?g,2.0 ?g or 4.0 ?g) could attenuate the infarct size, but the statistically significant effects of CCK-8 were obtained only at the doses of 1.0 ?g and 2.0 ?g(P

Objective To investigate the optimized parameters of dye (SYBR Green Ⅰ) realtime fluorescent polymerase chain reaction (RF-PCR) for detecting αβT lymphocyte clones in the peripheral blood and its application in monitoring specific T cell clone in the peripheral blood mononuclear cells (PBMC) from patients with chronic hepatitis B (CHB). Methods The total RNA was extracted from the PBMC of six healthy donors, and was reversely transcripted into cDNA. Then the cDNA was amplified using RF-PCR with the primers specific for T cell receptor β viable region (TCRBV) gene families as upstream primers and the primer for T cell receptor (TCR) β constant region (TCRBC) as downstream primer. The annealing temperature,concentration of primers and the total number of cycles were comparatively analyzed. The optimized PCR was performed to investigate the 24 TCRBV gene families from 12 patients with CHB, and the PCR products were monitored by melting curve analysis, and the clone expansion of peripheral blood T cell was detected by peak-motif of melting curve analysis. Results The optimized annealing temperature, final premier concentration,the number of cycles were 60.6 ℃, 0.5 μmol/L and 40 cycles, respectively. The begin temperature for melting curve analysis was better as 80 ℃ compared to 75 ℃. There was mono-peak on melting peak chart for TCRBV gene families in PBMC from patients with CHB, and PCR products of the single peak were determined as monoclonal T cell by sequencing. Conclusions The optimized reaction parameters of RF-PCR for monitoring 24 TCRBV gene families are determined. The melting peak chart could be used to monitor the clone expansion of the peripheral lymphocytes and to detect the clone-specific T cells in the peripheral blood from patients with CHB.

OBJECTIVE:To observe the effects and side effects of recombinant erytropoietin(rEPO)on patients with anemia in chronic renal failure(CRF).METHODS:128 patients with anemia in CRF had been given rEPO by subcutaneous injection for12weeks,the clinical effects were observed by own control method.RESULTS:Excellence cases amounted 91,efficacy cases33,and the overall efficacy rate was96.88%;The side effects included hypertension,coagulation in dialysis machine,pain in injection site and head,no severe adverse drug reactions were found;No degradation in renal function was found in non-dialysis patients during the medication.CONCLUSIONS:rEPO could improve anemia in CRF safely and effectively.

Cultivating the talents with scientific research and innovation has been the emphasis of medical education in 21st century.We make a deep exploration and practice on how to cultivate the innovative ability of undergraduates in functional experimental teaching.The article points out that it is an effective way to convert educational sense,to update educational mode,to strengthen the scientific research practice,and to enhance the innovative experiment.

Objective To investigate the incidence and risk factors of acute kidney injury (AKI) after craniocerebral injury.Methods A single cohort of 791 patients who suffered from craniocerebral injury from January 2008 to January 2010 in the Second Hospital of Lanzhou University were prospectively analyzed.Craniocerebral injury was defined according to definite medical history of craniocerebral injury,the verification of CT and Glasgow coma scale (GCS) score.AKI was defined as a relative 50％ increase or an absolute increment of 26.4 μmol/L in Scr within 48 hours and/or urine volume ＜0.5 ml·kg-1·h-1 up to 6 h.Multivariate Logistic regression analysis was used to evaluate possible risk factors associated with post-craniocerebral injury AKI.Results Of the 791 patients,the incidence of AKI was 39.4％.In hospital mortality of AKI patients was 27.9％,which was 5.065 times of non-AKI patients (P＜0.01).The incidence of AKI in patients with lower GCS score (≤8 score,heavy group)was 69.7％,which was significantly higher as compared to moderate and mild groups (P＜0.01).Unconditional multivariate Logistic regression analysis revealed that lower GCS score (≤ 8 score),hypotension (systolic pressure＜90 mm Hg),elderly and male were the independent predictors of AKI episodes,the corresponding OR values were 2.932,2.176,1.789,1.544 respectively.Conclusions AKI is a common complication after craniocerebral injury.Lower GCS score,hypotension,elderly and male are the independent risk factors of AKI in patients after craniocerebral injury.