1.BREEDING OF A GLUTATHIONE OVERPRODUCING STRAIN
Microbiology 1992;0(04):-
A zinc chloride and ethionine resistant mutant 0 5Eth400 5 was o btained from its parent strain Saccharomyces cerevisiae 346 by UV and 60 Co ? ray tr e atment and rational screening The glutathione productivity of the mutant reach e d 165 96mg/L by flask culture, which was 350% higher than the parent strain, an d the glutathione content in the dried cells reached 19 76mg/g, which was 318 6% higher than the parent strain A desecend of only 10 7% in the glutathione yie ld of the mutant was observed after ten times of subculture Therefore, the obtai ned mutant is stable strain that is worthwhile to be studied further
2.Prognosis of patients with acute ST-elevation myocardial infarction due to drug-eluting stent thrombosis
Qi ZHANG ; Jianping QIU ; Ruiyan ZHANG
Chinese Journal of Interventional Cardiology 2003;0(05):-
Objective To evaluate clinical and procedural characteristics and outcomes of patients with ST-elevation myocardial infarction(STEMI) caused by drug-eluting stent(DES) thrombosis undergoing primary percutaneous coronary intervention(PCI).Methods From October 2004 to June 2007,31 patients with STEMI due to definite DES thrombosis who received primary PCI were retrospectively studied(ST group).Baseline clinical,angiographic,PCI procedural features and 1-year outcomes were recorded.Another consecutive 93 patients with STEMI due to de novo coronary thrombosis were served as control group.The primary endpoint was cumulative rate of major adverse cardiac events(MACE),including death,reoccurrence of myocardial infarction and target vessel revascularization(TVR),during hospitalization and at 1-year follow-up.Results Compared with the control group,patients in ST group were older(69.9?11.4 y and 63.7?13.6 y,P=0.01) and higher comorbidity rates of diabetes(41.9% and 22.6%,P=0.04)and previous history of myocardial infarction(29.0% and 11.8%,P=0.02).The occurrence of TIMI 3 flow immediately after primary PCI was reduced(45.2% and 92.5%,P
3.Hospital infection in 360 patients with acute cevebrovascular diseases clinical analysis
Jianping YAN ; Dong LIN ; Desheng QIU
Chinese Journal of Primary Medicine and Pharmacy 2006;0(01):-
Objective To study the risk factors of nosocomial infections in patients with cerebrovascular disease and prevent and cure them.Methods A retrospective survey on the hospital infeation in patients with cerebrovascular disease in our hospital during March 2003 to March 2005 was couducted.Results The result showed that 61 of the 360 patients had hospital infection,the rate of infection was 16.94%,and the hospital average infection rate in all was 3.92%,hospital infection aging,treatment days in hospital,consciousness invasive operation Hospital infection was a retated.The main infection sites were at respirate tract,the most common bacteria were Gram-Negative bacilli.Conclusions There is higher hospital infection rate among,the acute cerebrvascular patients.Age,the critical patients,invading operations are prmincipal factors.We should prevent it in time.
4.Clinical Study of Tentorium Cerebelli Hiatus Incision for Treatment of Severe Brain Injury Complicated with Tentorial Herniation
Shaojian ZHEGN ; Zheng QIU ; Jianping XIONG
Journal of Chinese Physician 2001;0(06):-
Objective To explore the efficacy of tentorium cerebelli hiatus incision for treatment of severe brain injury complicated with tentorial herniation. Methods Tentorium cerebelli hiatus incision was performed in 38 patients of severe brain injury with secondary brain stem damage resulted from tentorial herniation after intracranial hematoma evacuation and bone flap craniotomy decompression. The changes of four sides cistern, ambient cistern and interpeduncular cistern before and after operation were observed. Results There was a siginificant difference in the appearance ratio of four sides cistern, ambient cistern and interpeduncular cistern between the preoperation and postoperation(P
5.An analysis of surveillance data of iodine deficiency disorders in Jiangxi Province in 2011
Zhihong LI ; Jun SHANGGUAN ; Jianping WAN ; Haiqing QIU ; Du CHEN
Chinese Journal of Endemiology 2014;33(3):304-306
Objective To analyze the monitoring data of iodine deficiency disorders in Jiangxi Province and to provide a basis for adjusting iodized salt concentration.Methods Thirty primary schools were selected in 30 counties and 40 pupils' goiters were examined with B ultrasound in every school.At the same time,salt iodine level was tested at their home.Twelve pupils urinary iodine in selected schools,15 pregnant urinary samples,15 lactating women urinary samples in 3 towns around the schools and water samples in the counties with the schools were collected.Results The goiter rate of children aged 8 to 10 was 1.25% (15/1 200); the median salt iodine was 30.80 mg/kg and the consumption rate of qualified iodized salt was 96.92% ; the median urinary iodine level of children,pregnant and lactating women was 308.73,206.95 and 206.75 μg/L,respectively.The proportion of urinary iodine level of children above 300 μg/L was 52.25%(186/356),while the proportion of urinary iodine level of pregnant women below 150 μg/L was 34.81%(157/451).The water iodine median was 4.62 μg/L and the per capita daily salt intake was 13.20 g in Jiangxi Province.Conclusions The status of iodine deficiency in Jiangxi has been significantly improved.According to the monitoring results,it's necessary to adjust salt iodization standards appropriately.
7.Survey of health adult serum N terminal pro brain natriuretic peptide concentration correlation of body level in Heyuan area
Guoquan ZHONG ; Jianping XU ; Yeshuang ZHANG ; Dandan QIU
International Journal of Laboratory Medicine 2015;(12):1730-1732
Objective To investigate the healthy adults in Heyuan area of serum N terminal pro brain natriuretic peptide (N‐proBNP) concentration distribution and the preliminary investigation on the normal reference range .Methods From 2010 Novem‐ber to 2012 November in the physical examination ,laboratory examination center of our hospital indicators ,ECG ,B ultrasound ex‐amination showed no abnormalities of the healthy people 1 017 ,according to the different gender and age :A group less than 25 years ,group B was 25- <35 years ,35- <45 years ,group C ,group D 45- <55 years ,55- <65 years of E group ,F group for over 65 years a total of 6 age groups ,content determination of serum N‐proBNP by Roche chemical instrument light .Results age ,gen‐der can affect the distribution of N‐proBNP level ,and with the increase of age ,adjacent to the age groups (except F group) to com‐pare the serum levels of N‐proBNP ,the difference was statistically significant (P<0 .05);Compared with the F group in each age group was significantly increased(P<0 .05) ,there was statistical significance compared with the group ;the serum N‐proBNP level of different sex ,the difference was statistically significant (P<0 .05) .Conclusion age ,gender and other physiological factors can affect the distribution of N‐proBNP levels ,should establish the corresponding reference interval according to the different age ,gen‐der ,provide the corresponding parameters for the diagnosis and treatment of related diseases .
8.Effects of mitofusin-2 gene on cell proliferation and chemotherapy sensitivity of MCF-7.
Yun, XIA ; Yaqun, WU ; Xiaojun, HE ; Jianping, GONG ; Fazu, QIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2008;28(2):185-9
In order to evaluate the effect of mitofusin-2 gene (mfn2) on proliferation and chemotherapy sensitivity of human breast carcinoma cell line MCF-7 in vitro, pEGFPmfn2 plasmid carrying full length of mitofusin-2 gene was transfected, by using sofast, into MCF-7 cells. Mitofusin-2 gene expression in MCF-7 cells transfected by sofast after 48 h was detected by PCR and Western blotting, and the stable expression of GFP protein in MCF-7 cells by Western blot analysis. The proliferation of MCF-7 cells was assayed by MTT and cell counting. By using PI method, the effects of mfn2 on the cell cycle distribution of MCF-7 were measured. Annexin-V/PI double labeling method was employed to detect the changes in apoptosis induced by chemotherapeutics before and after transfection. The results showed that the MCF-7 cells transfected with mfn2 gene could stably and highly express GFP protein. MTT assay revealed that after transfection of mfn2 cDNA, the proliferation of MCF-7 cells was significantly inhibited. DNA histogram showed that cells arrested in S phase, and the percentage of S phase cells was 42.7, 17.2 and 19.6 in mfn2 cDNA transfection group, blank plasmid transfection group and blank control group, respectively (P<0.05). The apoptosis ratio of the cells transfected with mfn2 gene was increased from 3.56% to 15.95%, that of the cells treated with camptothecin (CAMP) followed by mfn2 gene transfection was 69.6%, and that in blank plasmid transfection group and blank control group was 31.0% and 23.4% respectively (P<0.05). It was suggested that transfection of mfn2 gene could significantly inhibit the proliferation of MCF-7 cells and promote their sensitivity to CAMP with a synergic effect.
Antineoplastic Agents, Phytogenic/pharmacology
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Apoptosis
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Camptothecin/pharmacology
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Cell Cycle
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Cell Line, Tumor
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Cell Proliferation
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Drug Screening Assays, Antitumor
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Flow Cytometry
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Gene Expression Regulation, Neoplastic
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Green Fluorescent Proteins/metabolism
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Membrane Proteins/*biosynthesis
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Membrane Proteins/*genetics
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Mitochondrial Proteins/*biosynthesis
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Mitochondrial Proteins/*genetics
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Transfection
9.Application of focused preventive nursing for ocular complications in patients undergoing general anesthesia prone surgery
Huimin QIU ; Jianping FENG ; Juan XU ; Jian CHEN
Chinese Journal of Practical Nursing 2021;37(3):197-200
Objective:To explore the effect of application in patients undergoing general anesthesia prone surgery on basis of observation of ocular complications and focus on preventive care.Methods:One hundred patients who underwent general anesthesia prone surgery before the start of the ocular complications focused preventive care exploration activity (January to May 2019) were set as the control group. After the ocular complications focused preventive care exploration activity was launched (June to October, 2019) 100 cases of general anesthesia prone surgery in our hospital were set as the experimental group, and the observation indexes after the two groups were compared.Results:The satisfaction scores of nurses and patients in the observation group were (9.40 ± 0.57) and (9.30 ± 0.56) respectively, while those in the control group were (7.51 ± 0.88) and (7.09 ± 1.10) respectively, the difference between the two groups was statistically significant ( t values were 18.013 and 13.063, all P < 0.05). The incidence rates of conjunctival congestion, edema and exposure keratitis in the observation group were 4.00% (4/100), 2.00% (2/100) and 1.00% (1/100), respectively, while those in the control group were 12.00% (12/100), 10.00% (10/100) and 9.00% (9/100), respectively. the difference between the two groups was statistically significant ( χ 2 values were 4.348, 5.674, 6.737, all P< 0.05). Conclusion:The focus on preventive care of ocular complications in patients undergoing general anesthesia prone surgery can significantly reduce the risk of ocular complications in this type of patients, and it is well recognized by both patients and patients.
10.Enhanced inhibitory effects of co-adiministration of Astragalus Saponin Ⅰ and bendazac lysine on hypertrophy of cultured rat mesangial cells
Xiaoxing YIN ; Yindi ZHANG ; Junxian YU ; Jianping SHEN ; Jun QIU
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To investigate the effects of co-administration of Astragalus Saponin Ⅰ(ASI)and bendazac lysine(BDL)on hypertrophy of cultured rat mesangial cells and its mechanism.Methods The levels of collagen Ⅳ and laminin,the percentages of cells in S phase,the relative quantity of transforming growth factor ?1(TGF-?1) mRNA and indexes of oxidative status were assayed after the cells were incubated in different agents for 36 h.Results The percentage of S phase cells in high glucose group(HG) was greatly decreased while those of vitamin E group(VE) and co-administration groups were increased.The relative quantity of TGF-?1 mRNA and the collagen Ⅳ level in co-administration groups were significantly decreased,and the levels of total anti-oxidative capability(T-AOC),activity of catalase(CAT),GSH-PX,and SOD were greatly increased.Furthermore,the significant differences were found between low ASI(AL)group,low BDL(BL) group and co-administration of low ASI and low BDL(AL+BL) group for TGF-?1 mRNA,T-AOC and GSH-PX;the high ASI group(AH),high BDL group(BH) and co-administration of high ASI and high BDL group(AH+BH) for TGF-?1 mRNA and collagen Ⅳ,respectively. Conclusion Co-administration of ASI and BDL has synergetic effects on regulating TGF-?1,collagen Ⅳ,and radical oxidative stress,therefore,is beneficial to protecting rat mesangial cells against hypertrophy.