Objective To explore the character of the myelodysplastic syndrome(MDS) with autoimmune disorders (AID).Methods The clinical data of 60 MDS patients were reviewed.Results 5 of 60 MDS patients had AID ,of which 3 had Graves' disease.The commonest MDS was refractory anemia with ringed sideroblasts (RAS).Conclusion MDS might occur with Graves' disease.Humoral immunological abnormality might play an important role in MDS patients with autoimmune disorders and FAB types might not be associated with this.

Objective To investigate the value of MR multiparameter imaging for the clinical classification of chronic pancreatitis. Methods 65 patients with confirmed chronic pancreatitis by follow-up and pathologic examinations ( 14 mild, 37 moderate and 14 severe according to MANNHEIM system) and 20healthy volunteers were included in this study. MR examination including routine T1WI, T2WI, MRCP and dynamic enhanced MRI. The data were measured and statistical analysis was applied in four groups. Two radiologists assessed pancreatic duct diameter, pancreatic size, pancreatic cyst, pancreatic stone and pancreatic signal intensity on MRCP, T1-weighted and T2-weighted images. Pancreatic signal intensity were also measured on dynamic enhanced MR. Results Mean values of pancreatic signal intensity ratio on T1WI (rT1)in the pancreas were significantly reduced in patients with moderate and severe CP compared with volunteers.There was significant difference among four groups ( normal, 0. 98 ±0.27; mild, 0. 84 ±0. 12; moderate,0.81 ±0. 16; severe, 0.75 ±0.24). Mean values of pancreatic signal intensity ratio on T2WI (rT2) in the pancreas were no difference among four groups ( normal, 1.28 ± 0.3; mild, 1. 46 ± 0. 44, moderate, 1.46 ±0.55; severe, 1.76 ± 0.72). Pancreatic duct diameters were significantly increased in mild, moderate and severe CP groups [ mild (5.3 ± 2.4) mm; moderate ( 6.5 ± 3.3 ) mm; severe ( 8.1 ± 4.1 ) mm ] compared with patients without CP[ (2.0 ±0.6) mm; P < 0.01 ]. Severe degree of Cambridge classification was graded as mild in 4 (29%), moderate in 33 (89%), severe in 13 (93%). Pancreatic calcification was graded as mild in 2 ( 14% ), moderate in 11 (30%), severe in 5 (36%). Pancreatic pseudocyst was graded as mild in 0, moderate in 6 ( 16% ), severe in 3 (21.43%). Pancreatic parenchymal atrophy was graded as mild in 4 (29%), moderate in 22 (59%), severe in 10 (71%). They did not vary among CP groups. Parenchymal/arterial phase enhanced ratio (P/A) in the pancreas were significantly increased in patients with mild,moderate and severe CP (mild, 1. 10 ±0.08; moderate, 1.37 ±0.15; severe, 1.48 ±0.53) compared with patients without CP (0.88 ± 0.08, P < 0. 05 ). Significant correlation was present between the severity level of CP and the change of rT1, severe degree of Cambridge classification, the pancreatic duct diameter and P/A (r=0. 34, 0.41, 0. 62, - 0. 43; P < 0. 01 ). ROC analysis showed the presence of pancreatic duct diameters more than 2.5mm, rT1 less than 0. 8 and P/A more than 0. 8 had a sensitivity and specificity of diagnosing chronic pancreatitis of 94% and 79%, 90% and 48%, 95% and 47% respectively. Combined with the three variables, the specificity of diagnosing chronic pancreatitis can be improved to 95%.Conclusions T1-weighted, MRCP and dynamic enhanced MRI imaging can accurately evaluate the clinical severity of chronic pancreatitis. MRCP had the highest sensitivity and specificity, followed by T1-weighted and dynamic enhanced MRI imaging.

Objective To investigate the expression of myeloid antigen(MyAg) in acute lymphoblastic leukemia (ALL) and its correlation with clinical characteristics and prognosis.Methods The clinical data of 103 newly diagnosed ALL 1patients were retrospectively analyzed.These patients were divided into myeloid antigen-positive group (MyAg+ ALL) and myeloid antigen-negative group(MyAgALL) based on the flow cytometry(FCM) analysis in bone marrow.The correlation of MyAg expression with the clinical features,short-term efficacy and 5-year overall survival were assessed in the two groups.Results MyAg expression was detected in 46.5％ out of 103 cases and CD13,CD33 were the most commonly expressed MyAg(34.2％ and 21.4％ respectively).The expression of CD34 in MyAg+ ALL group was higher than MyAgALL group(75.3％ vs 60.5％,P ＜0.05).The expression of MyAg in T-ALL group (66.7％) was higher than B-ALL group (42.8％) (P ＜ 0.05).The clinical and biological characteristics of ALL patients between MyAg+ and MyAggroups showed that higher percentage of incoincidence of classification between morphology and immunology.Conclusion MyAg expression was not correlated with clinical characteristics and prognosis of ALL patients.

OBJECTIVE:To assess the cost and therapeutic effects of Chinese traditional medicine and western medicine in treating varicocele sterility. METHODS:165 patients with sterility were randomly divided into 2 groups. Chinese traditional medicine group included 110 cases who took Zhang's varicosity prescription, while the western medicine group included 55 cases who took human chorionic gonadotropin, clomiphene citrate, zinc gluconate, ATP and vitamin AD,Vitamin E,Vitamin C,for 3mo～9mo normally. The therapeutic effects and adverse effects of two groups were monitored and evaluated with cost- effectiveness analysis. RESULTS:The total effective rates of Chinese traditional medicine and western medicine were 81.82%and 50.91% respectively, while the costs were 3 688.2 Yuan and 2 399.2 Yuan accordingly, C/E were evaluated to be 45.08 and 47.13 respectively and ?C/?E was 41.7. CONCLUSION:Zhang's varicosity prescription is the better choice for treatment of varicocele sterility

Objective To investigate the anxiety status of male partner involved in induced abortion and to analyze influencing factors related.Methods Male partners of the patients undergoing induced abortion in the gynecology and obstetrics department of a hospital in Kunming in April and May in 2015 were selected and a questionnaire survey was conducted.Self-rating Anxiety Scale (SAS) was used to measure the anxiety status and SPSS 17.0 was used for statistical analysis.Results A total of 53 out of 205 male partners were found to have anxiety symptoms (25.9％),with 44 cases of mild anxiety (21.5％),8 cases of moderate anxiety (3.9％) and 1 case of severe anxiety (0.5％).The main related risk factor was times of pregnancy (OR=3.35) while the protective factors were level of education (OR =0.60),times of induced abortion (OR =0.20) and age (OR =0.32) Conclusion When abortion occurs,the male partner's anxiety appears,mainly with mild and moderate one,and female's emotion are also affected.It is important to strengthen health education and popularize contraceptive knowledge so as to reduce the incidence of anxiety.

AIM: To detect the expression level of telomere regulating factor tankyrase and its clinical significance in the origin and development of gastric adenocarcinoma. METHODS: Quantitative Real-time PCR was used to measure the expression of tankyrase mRNA in cancerous and normal adjacent tissues from 16 patients with gastric adenocarcinoma. Immunohistochemistry was used to detect tankyrase protein expressions in 37 gastric adenocarcinoma samples and 13 normal controls. RESULTS: The expression of tankyrase mRNA in gastric adenocarcinoma [median 1.44?10~ -2, range (3.88?10~ -5)-0.4847] was significantly higher than that in normal adjacent mucosa [median 1.0134?10~ -2, range 0-(4.933?10~ -2)] (P

ObjectiveTo investigate the clinical therapeutic effect and safety of solifenacin suceinate in the treatment of overactive bladder symptom after benign prostatic hyperplasia (BPH)operation.MethodsAmong 115 patients receiving BPH surgery,58 cases were given solifenacin at bedtime 4 d after surgery for 20 d (treatment group),and 57 cases were given anisodamine after surgery (control group).The urination of patients before and after pulling out catheter were observed,and urodynamic examination,international prostatic symptoms score (IPSS) and overactive bladder symptom score (OABSS) were used to evaluate urination status.ResultsIn treatment group,IPSS and OABSS decreased from 28.3 to 11.3 score and from (14.2±1.2) to (2.9±0.7) score before and after treatment,respectively (P＜0.01).In control group,IPSS and OABSS decreased from 27.3 to 11.8 score (P＜0.01) and from (14.2±1.6) to (11.3±1.1) score before and after treatment,respectively (P＞0.05).ConclusionsThe proper use of solifenacin after prostate operation may release bladder distress and facilitate rehabilitation in patients with overactive bladder symptom.

Objective To identify epitope of neutrophil-activating protein(NAP) of Helicobacter pylori(Hp).Methods Using the mouse monoclonal antibodies against NAP as selective molecular and immunoscrcening phage-display random 7-peptides library.The positive clones were sequenced and analyzed.Phage clones were chosen to immunize mice and to evaluate the potential of phagotopes as effective vaccines.Results One mimotope(FAHLATQ)showed a good match with the NAP at 140-143 AA(AHLA)and the serum of mice induced by the phage clone clearly recognized NAP.Conclusion This study suggests thatthe antigenic epitope could be mapped through screening the phage-display peptide library with monoclonalantibody and a mimotopo of NAP providing an ahernative approach for the diagnosis and development of avaccine for Hp.

AIM: To study the expression of telomerase inhibitor Pinx1 in acute leukemia cells and during the differentiation of acute promyelocytic leukemia cells, and to realize its effect on telomerase activity. METHODS: Realtime quantitative PCR with fluorescence probe hybridization was used to measure the expression of Pinx1 and hTERT mRNA in acute leukemia cells and during differentiation of NB4 cells induced by ATRA. The correlations between Pinx1 and hTERT expression were also analyzed. RESULTS: Pinx1 mRNA expression in acute leukemia samples (0.00312, 5.42?10 -4-0.024) was significantly higher than that in normal bone marrow mononuclear cells (7.89?10 -4, 0-0.00863, P

AIM:To study the telomere maintenance mechanism in mesenchymal stem calls(MSCs).METHODS:MSCs were isolated from healthy human bone marrow by their adherence to plastic and then were checked with CD14-FITC,CD45-FITC,CD44-FITC,HLA-DR-FITC,CD34-PE,CD29-PE and CD166-PE.Telomere length and ECTR DNA in MSCs were detected by Southern blotting.The localization of TRF1 and promyelocytic leukemia(PML)in MSCs were detected with immunofluorescence staining.TRAP protocol was performed to detect the telomerase activity in MSCs and MSCs-derived adipocytes.Western blotting and TRAP protocol were applied to measure telomerase activity of MSCs,which were synchronized by serum starvation and aphidicolin treatment.RESULTS:The telomere in length seemed shorter and relatively more homogeneous in MSCs and HeLa cells than that in WI-38-2RA cells.TRF1 did not concide with PML nuclear body in MSCs and HeLa cells while it exclusively did in WI-38-2RA cells.ECTR DNA was negative in MSCs and HeLa cells but positive in WI-38-2RA cells.Telomerase was negative in MSCs but it was positive in MSCs-derived adipocytes detected by TRAP.Moreover,a cell cycle-dependent expression profile of telomerase was found in MSCs when they were synchronized by serum starvation and aphidicolin treatment.Untreated MSCs expressed very low level of telomerase probed by Western blotting with 2C4 mAb,but the telomerase level had significantly increased when these cells were trapped in S phase.CONCLUSION:The telomere of MSCs is maintained by telomerase pathway instead of alternative lengthing of telomere(ALT)and the level of telomerase expression is associated with cell cycle stage.[