Objective To investigate the relationship of nerve growth factor (NGF) between serum and follicular fluid in patients with polycystic ovary syndrome (PCOS), and its effect on pregnancy outcomes. Methods From December 2011 to November 2012, 65 PCOS patients suffered with in vitro fertilization-embryo transfer (IVF-ET) were included in PCOS group and 31 non-PCOS patients with IVF-ET were used as control group. The expressions of NGF in serum and follicular fluid were detected by ELISA on the injection day of chorionic gonadotropin (HCG). The expression levels of NGF in serum and follicular fluid were compared between PCOS group and control group. Results The NGF levels in serum[(14.38±0.42) ng/L] and follicular fluid[(9.61±0.49)ng/L] were significantly higher in PCOS group than those of control group[(11.39±0.38) ng/L and (7.55 ± 0.40)ng/L]. In PCOS group, NGF levels in serum [(14.22 ± 0.35)ng/L] and follicular fluid [(9.30 ± 0.31)ng/L] were significantly lower in pregnant group than those of non-pregnant group[(14.51±0.43)ng/L, (9.86±0.46)ng/L, P<0.01]. Conclusion The increased levels of NGF in serum and follicular fluid of PCOS patients contribute to the pathogenesis of PCOS. The high expression of NGF in serum and follicular fluid may be harmful to pregnancy in PCOS patients.

Objective To assess the value of CT in diagnosis of recurrent rectal carcinoma.Methods Forty seven patients who had undergone locally curative surgery were examined by CT.All patients received intravenous contrast medium and two oral doses and a intrarectal doses of 500 ml 3% Ultravist were given.The characters of benign and malignant lesions were analysed in order to evaluate the value of the classification on CT in the differentiation of benign masses from malignant ones.Results 26 local recurrences occurred in 47 cases,including 13 cases with an round preasacral mass,8 cases with an irregular rectal wall thickening,2 cases with a flat preasacral mass,3 cases with a preasacral mass with low attenuation.The degree of enhancement of local recurrent masses was higher than that of benign masses.Conclusion The features of recurrence of rectal carcinoma on CT is an round or irregular asymmetrical preasacral mass or an irregular rectal wall thickening.It is useful way in diagnosis of recurrence of rectal carcinoma.

ObjectiveTo summarize the nursing experience of postoperative liver biopsy in patients after liver transplantation. Methods89 patients undergoing postoperative liver biopsy after liver transplantation from June 2010 to April 2011 were retrospectively analyzed.Their nursing experience was summarized. Results81 patients were confirmed of having rejection reaction.89 patients had no severe complications,only 28 cases(31.5％)had mild local pain. ConclusionsSufficient preoperative preparation,good nursing cooperation during the operation,close postoperative observation and nursing can prevent and timely detect and cope complications,thereby reduce the risk of liver puncture.

Objective To improve students' nursing skills by studying on the management method of skill training after class. Method 2 random sampled classes were investigated. One class was experimental group, and received student-oriented management method. The other was control group, and received ordinary management method. Result The experimental group was different from control group in training attendance, number of questions, theory and skills examination performance. There was significant difference between them. Conclusion Student-oriented management approach improves students' training interesting, students' self-management ability, organizational capabilities, ability of expression and critical thinking.

Nanomedicine offers great promise for early diagnosis and treatment of formidable diseases. The unique morphology and biology characteristics of bacteriophage provide unprecedented opportunity for such endeavor. The paper summarizes the application of bacteriophage in nanobiomaterials, nanomedicine, nanomedicine delivery and nanodiagnosis, especially the nano-imaging reagents and future direction concerning nanomedicine based on bacteriophage.

The rate of stroke has been rising year by year and become the world's major cause of mortality and disability. The high rate of disability did not only bring economical burden to society and family, but also induce psychological damage to patients. Post-stroke depression (PSD) is a most common mental complications of stroke. In recent years, More and more attentions have been paid to PSD. This article reviewed the cause and mechanism of PSD and the treatment with Chaihu Shugan Powder on it.

We find that there is a correlation among thesize of ventricular septal defect (VSD) (X_2), PaO_2(X_3), V. /V. (X_7) and pulmonary systolic pressure(Y_1), diastolic pressure (Y_2), mean pressure (Y_3)by multivariate stepwise regression analysis, andso there are their parameters in the regression e-quations. The regression equations are Y_1 = 13. 24+ 0. 22X_2 - 0. 09X_3 + 0. 14X_7; Y_2 = 0. 65 + 0. 24X_2+ 0. 12X_7; Y_3 = 3. 54+ 0. 17X_2 + 0. 16X_7. But thecourse of the disease, ∑9 leads QRS dimension,R. /R., L. /L. are non-dominant factors, and sothere are not their parameters in the regression e-quation. The above-mentioned equations mayavoid the patients. with mild, moderate pulmonaryhypertension to have invasive examination, and re-duce its complications.

Objective To compare the effect of two different time for intravenous dexmedeto-midine alleviates shivering during parturients cesarean undercombined spinal-epidural anaesthesia. Methods One hundred and twenty parturients delivery under combined spinal-epidural anaesthesiawere equally randomized into three groups.The parturients were intravenously injected with dexmedetomidine 0.4 μg/kg (15 min)at 15 min before the beginning of anesthesia (group A)or the time immediately after delivery of fetus (group B)respectively.Moreover,group C was the control group.Parturientsin group C were given equal volume of normal saline.Mean arterial pressure (MAP),heart rate(HR),pulse oxygen saturation (SpO 2 )and ear temperature were recorded respec-tively.Apgar scores of the fetus at 1 min and 5 min,the incidence and grade of shivering response, Ramsay sedative grade and adverse responses such as nausea,vomiting and traction reaction were re-corded as well.Results By 30 min after operation,2 parturients (5%)in group A,8 parturients (20%)in group B and 19 parturients (47.5%)in group C experienced shivering.Traction reaction was observed in 2 parturients (5%)in group A,in 3 parturients (7.5%)in group B and in 10 parturi-ents (25%)in group C respectively.Compared with group C,the incidence rate and degree of shive-ring were significantly lower in groups of A and B (P <0.05),as well as the traction reaction (P <0.05).The incidence rate and degree of shivering in group A were significantly lower than that in group B (P <0.05),but the incidence rates of traction reaction were not statistically different between the two groups.Conclusion Dexmedetomidine administered before combined spina-epidural anesthesia could prevented shivering response in parturients undergoing cesarean section better than being injected immediately after the birth offetus.

Abstract] Objective To establish an assay for the detection of porcine parvovirus ( PPV) and to verify its application for monitoring cells used for production.Methods A pair of primers and one probe were designed according to the conserved sequence encoding non-structural protein 1 (NS1).Based on the designed primers, a real-time fluorescent quantitative PCR assay for the detection of PPV was developed. Several parameters including the linearity, precision, minimum detection limit and anti-interference of the established assay were evaluated.A stock of PPV strains was prepared by infecting swine testicle ( ST) cells with PPV strains.An assay for the detection of PPV infection was developed by using ST cells as sensitive cells.A combined ST cell infection-PCR test was developed by combining the ST cell infection assay with the real-time fluorescent quantitative PCR assay.The sensitivity of ST cell infection-PCR test was analyzed.The cell samples used for production of biological products were detected by using the established assay.Results The real-time fluorescent quantitative PCR assay was specific for the detection of PPV without cross-reaction to other species of parvovirus virus, SV40 virus and other porcine viruses.The linear range of the assay was 1×109-1×104 copies/μl with a R2 value more than 0.98.The sensitivity of the real-time quantitative PCR assay was 1×104 copies/μl.Both of the intra-and inter-coefficient of variation (CV) were less than 5%in Ct values.The intra-and inter-CV in copies of detection were 5%-15% and 30%-40% respectively.The minimum detection limit of the real-time fluorescent quantitative PCR assay was 1CCID50/ml.The PPV strains were detected in cell samples with no interference.The sensitivity of ST cells infection-PCR test was 0.01CCID50/ml.All of the 22 cell samples were negative for PPV by using the real-time fluorescent quanti-tative PCR assay.Conclusion The real-time fluorescent quantitative PCR and the ST cell infection-PCR test for the detection of PPV in cells were established successfully.The application of the two assays was conducive to further enhance the safety of using cells for production and therapy.

Objective To investigate the value of spiral CT in diagnosis and its differiential diagnosis of adrenal hyperplasia char-acterized with solitary nodule.Methods 20 cases of adrenal hyperplasia characterized with solitary nodule and 28 adrenal adenomas confirmed by pathology underwent routine CT plain scan and multiphase enhanced CT scan.The density of nodule,capsule and de-gree of enhancement in each period were respectively analyzed and compared with the normal adrenal.The difference of CT value be-fore and after enhancement(△CT value)of the both was compared using pairing t test.Results 20 cases of adrenal hyperplasia nod-ules had no clear boundary with normal adrenal.Of which 1 5 cases showed isodensity,and their degree of enhancement was synchro-nized with normal adrenal;4 cases showed lower density,of which 2 had no enhancement,the other 2 obviously enhanced,but lower than normal adrenal;1 case showed mixed density mass,mainly composed of soft tissue,fat and punctuate calcification could be seen in it.Enhanced △CT values of 20 cases of adrenal hyperplasia nodule and normal adrenal were compared respectively,and there was no significant difference(P >0.05).28 cases of adrenal adenomas presented lower density than that of normal adrenal and had obvi-ous boundary between them.Enhanced △CT values in arterial phase of the lesion and normal adrenal showed significant statistically difference (P <0.05).Conclusion CT performance of adrenal hyperplasia with solitary nodule has specific characteristics,the densi-ty,capsule and enhanced mode will be helpful in differentiating from adrenal cortical adenoma.