1.Stimulating activity of Acanthopanax gracilistylus anti-tumor component on monocytes
Baoen SHAN ; Jianping DUAN ; Lihua ZHANG ; Al ET
Chinese Journal of Immunology 2001;0(07):-
Objective:Study the effect of Acanthopanax gracilistylus extract(Age) on monocyte,and reveal the anti trmor mechanism.Methods:The content of TNF ?,IL 12 were examined by using biological method and ELISA,respectively.The expression of monocytic cytokine mRNA are investigated by RT PCR.The effect of Age on the phagocytosis of monocytes is studied using TdR incorporation method.Results:After cultured with Age,the production of cytokines by monocytes such as TNF ?,IL 12 are markedly increased with good dose dependent manner.After monocytes cultured with LPS in the presence or absence of Age,Age markedly enhances LPS induced TNF ? production.Treatment with Age,the production of cytokines and the expression of their mRNA are increased simultaneously.The results in vivo also show:Age can enhance the productions and the expressions of TNF ? and IL 12.Conclusion:The anti tumor active component of Age has obvious stimulative activity on monocytes, increasing the production of cytokines, enhancing the phagocytosis.These results product the scientific evidence for the study of anti tumor mechanism and clinical ultilization.
2.The research of the relationship between HBV genotypes and YMDD motif mutations as well as basic core promoter mutations
Jianping DUAN ; Kun ZHU ; Weijia WU ; Xu HU ; Yuexiang CAI
Chinese Journal of Laboratory Medicine 2011;34(1):68-72
Objective To investigate the relationship between HBV genotypes and YMDD motif mutations or BCP mutations in Xiangtan of Hunan Province. Methods HBV genotypes, YMDD motif mutations and BCP mutations were analyzed in 952 HBV infected patients. Results HBV genotyping showed that 698 HBV type B patients and 115 HBV type C patients accounted for 73.32% and 12.08% respectively of all the participants. The rest 139( 14.60% )were genotype B and C mixed infection( B + C ). The analysis of YMDD motif mutations showed that 844 YMDD wild-type which accounted for 88.66% of all the subjects and the remainder were YMDD mutation types, of which 54( 5.67% ) carried YVDD, 53( 5.57% ) YIDD,and 1 YVDD and YIDD mixed infection. Basic Core Promoter mutations showed that 1762A/1764G ( wild type )accounted for 70.59% and 1762T/1764A( mutant ) accounted for 19.75%. The rest 92 patients were 1762T/1764A and 1762A/1764G mixed infection. This study showed no significant difference in the rate of YMDD mutation( 10.04% vs 10.43% ,χ2 =0.017,P>0.05 ) ,but a significant difference in the types of YMDD mutation(χ2 = 4.836, P < 0.05 )between HBV types B and C. The YVDD mutation was more commonly seen in genotype C( 9.57% ) than in genotype B( 5.88% ). The BCP mutation rate showed a significant difference( 27.36% vs 46.09%, χ2 = 16.478, P < 0.01 ). Genotype C was more frequent than genotype B. The BCP mutation rate showed no significant difference between YMDD Wild-type and YMDD mutation types( 28.67% vs 35.51%, χ2 = 2.139, P > 0.05 ), but most of BCP mutations happened in YVDD mutant type( 61.11% ). Conclusions ( 1 ) The predominant HBV genotypes in Xiangtan were genotype B and genotype C, the major genotype was type B, which display the characteristics of epidemiology in Southern China. ( 2 ) Determination of HBV genotypes before lamivudine therapy was probably not an important pretreatment investigation to predict antiviral responses. ( 3 ) Detection of HBV genotypes, YMDD motif mutations and BCP mutations will contribute to the correct evaluation of prognosis and timely proper management of HBV patients.
3.Investigation of self-perceived burden among continuous ambulatory peritoneal dialysis patients
Meiling XIAO ; Jianping HU ; Yannan LIU ; Shuping ZHENG ; Gongxiang DUAN
Chinese Journal of Practical Nursing 2013;29(28):67-69
Objective To investigate the level of self-perceived burden(SPB) and analyze the influencing factors in continuous ambulatory peritoneal dialysis(CAPD) patients.Methods One hundred and ten CAPD patients were investigated by a self-designed demographic questionnaire and Self-Perceived Burden Scale (SPBS).Results The total score of SPBS was (30.27±7.88) points,being in a middle level,and 85.45% of the patients showed different levels of burden.Single factor analysis showed the influencing factors of self-perceived burden were marital status,family per capita income,the main caregivers and selfcare ability.Conclusions Most CAPD patients have obvious self-perceived burden,nursing staff should use the relevant nursing measures to reduce the SPB with CAPD patients and improve their quality of life.
4.Teaching reform of diagnostics based on clinical skill competition
Bo HUANG ; Yuanfang DUAN ; Hong MEI ; Jianping XIE
Chinese Journal of Medical Education Research 2017;16(4):346-350
In view of the current situation of the teaching of diagnostics, this paper analyzes the ex-isting problems and deficiencies , and puts forward the teaching reform of the National Medical College Students' clinical skills competition. Through the construction of experimental teaching demonstration center of clinical skills, we set up theclinical skills associationamong the students, boldly try innovative training, strengthen humanistic education and take other measures to improve the quality of clinical practice teaching of diagnostics, so as to achieve the purpose of the competition focusing on promote teaching, learning and using by competition.
5.Preparation and transdermal resorption of tanshinone ethosomes in vitro
Xiaohong LU ; Jianping LIU ; Hua ZHAO ; Fan DUAN
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To prepare ethosomes and study on transdermal resorption of tanshinone ethosomes in vitro. METHODS: Tanshinone ethosomes were prepared by thin-film ultrasonic method.The different dosage regimens and volume on the transdermal resorption of tanshinone were evaluated through excised mice skin in vitro using modified Franz-diffustion cells. RESULTS: The mean particle diameter of ethosomes was 79.7 nm,and the encapsulation efficiency was about 81.2%.Cumulative penetration amount of tanshinone from ethosomes was 1024? 104.0 ng/cm2 and skin residual amount was 345.2?58.0 ng/cm2.Cumulative penetration amount increased with ethosomes volume.Liposomes failed to promote the penetration of tanshinone,and its skin residual amount was 625.3 ?124.5 ng/cm2,water-ethanolic suspension also could not promote the penetration of tanshinone,and skin residual amount was 765.0?60.00 ng/cm2. CONCLUSION:Ethosomes can improve significantly the transdermal delivery of tanshinone.
6.A study of tracking the superparamagnetic iron oxide and enhanced green fluorescent protein labeled miniature porcine bone marrow stem cells by in vitro MRI
Kuanxiao TANG ; Yunfeng SHEN ; Xubin YANG ; Huimin GU ; Hejiang DUAN ; Zhaoxia YAN ; Jianping WENG
Chinese Journal of Internal Medicine 2011;50(4):322-327
Objectives To track bone marrow stem cells (BMSCs) labeled by enhanced green fluorescent protein (EGFP) and superparamagnetic iron oxide ( SPIO ) -poly-L-lysine (PLL) compound by MRI in vitro for autotransplantation into pancreas of type 1 diabetes miniature pigs. Methods The BMSCs were isolated by density gradient centrifugation and attachment culture from type 1 diabetes minipigs' bone marrow. Expressional intensity of EGFP in BMSCs transfected lentivirus-EGFP with a multiplicity of infection Different magnetic resonance scanning protocols were carried out on various density BMSCs labeled by different concentration of SPIO in various time-point in vitro. Results When SPIO concentration was 25mg/L (count in Fe3 + ), the positive Fe3+ -labeling rate of BMSCs was 93. 1%. Most of SPIO particles in BMSCs' cytoplasm were observed in secondary lysosomes, but they were not detected in important organelle as cell nucleus. Comparing with gelatin the MRI of BMSCs labeled with SPIO in the condition with 1 ×104/ml cells density and 25 mg/L Fe3+ concentration in vitro, the signal intensity changes (△SI) after BMSCs labeled with SPIO 3 weeks and 6 weeks in TSE T1WI, TSE T2WI and FLASH T2 * WI sequences were 12%, 41%, 63% and 7%, 28%, 46% respectively (P < 0.01 and P < 0.05, respectively).Conclusions The data showed that the porcine BMSCs labeled with SPIO and EGFP could be traced successfully in vitro by MRI in the suitable sequences.
7.Effects of social skills training on subjective life satisfaction of long-term hospitalized patient with chronic schizophrenia
Jianjie WU ; Nian ZHU ; Yuanqing DUAN ; Jianping ZHANG ; Peiyan ZHANG ; Xiaojun WANG
Chinese Journal of General Practitioners 2009;8(9):624-627
han that in the control one (t=3.94, P<0.01).Conclusion Social skills training can effectively improve subjective satisfactions of the long-terra hospitalized patients with chronic schizophrenia.
8.Progenitor cells in mammalian maxillofacial and mandibular processes Source and differentiation phenotype
Zeyuan ZHOU ; Yan JIN ; Shuxiang YU ; Junnan SHI ; Jianping ZHANG ; Xiaoyan DUAN
Chinese Journal of Tissue Engineering Research 2010;14(50):9493-9496
BACKGROUND: It remains unclear whether ectomesenchymal cells also derived from neural crest stem cell in mammals.OBJECTIVE: To understand the specific markers and differentiation directions of maxillofacial and mandibular processes progenitor cells,and to explore the source and differentiation phenotype of ectomesenchymal stem cells.METHODS: The expression and changes of expression profiles of rat ectomesenchymal cells at E9.5,E10.5,E11.5,and E12.5days were observed by immunohistochemistry and flow cytometry.RESULTS AND CONCLUSION: The progenitors expressed multi-lineage markers,including neural system and several rnesenchymal tissue types,importantly the facts that molecule profiles were changed with time prolonged,suggesting these progenitors were in active differentiating stage,so they were stem like cells or contain stem like cells.Moreover,small populations(2%-3%)of CD57 and P75 phenotypes were detected by flow cytornetry,suggesting that ectomesenchymal stem cells were derived from neural crest,which maintained a quantitative stabilization though it is gradually differentiate after localization.
9.Association of interleukin-13RS20541 gene polymorphism with enterovirus 71 infection in children with hand-foot-mouth disease
Meihua KANG ; Xiaohui GONG ; Tuoyu GAN ; Cheng WANG ; Jianping DUAN ; Lixia CHEN
Chinese Journal of Applied Clinical Pediatrics 2017;32(10):763-766
Objective To explore the relationship between interleukin(IL)-13 RS20541 gene polymorphism and susceptibility of enterovirus 71 (EV71) infection in children's hand-foot-mouth disease.Methods Blood samples were collected from 123 children with EV71 infection from the Central Hospital of Xiangtan (experimental group),and they were divided into mild EV71 infection group (n =62) and severe EV71 infection group (n =61) according to their severity.And 52 healthy children without EV71 infection were selected as the controls,with age and sex matched.Two mL blood samples stored in the-80 ℃ freezer,were collected from all subjects,and DNA was extracted by Beijing ubiolab genetic technology company limited.The SNaPshot was used to determine genotype for G/A polymorphism at RS20541 position of IL-13 gene.SPSS 18.0 software was used to analyze the data.Results IL-13 RS20541 loci had 3 genotypes:AA,GA,GG;the frequency of AA,GA,GG in the experimental group was 4.07%,44.71%,51.22%,which was significantly lower than that in the healthy control group (the frequency of AA,GA,GG were 11.54%,32.69%,55.77%),there was no statistically significant difference in genotypes (x2 =4.676,P > 0.05);there was no statistically significant difference in allele frequency (the frequency of A,G in experimental group was 26.42 %,73.58 %,and that of the healthy control group was 27.88 %,72.12 %;x2 =0.080,P > 0.05).EV71 infection caused by mild group,severe group and healthy controls genotype frequencies between the 3 groups [(AA + GA) were 53.22%,44.26%,44.23%;GG were 46.78%,55.74%,55.77%;x2 =1.294,P > 0.05] and allele frequency (A were 30.65%,22.13%,27.88%;G were 69.35%,77.87%,72.12%;x2 =2.349,P > 0.05) among the mild group,severe group and healthy control group had no statistical significance.Conclusion There is no correlation between the IL-13RS20541 gene polymorphism and EV 71 infection in children with the hand-foot-mouth disease.
10.Effects of Abiotic Elicitors MeJA and SA on Alkaloids Accumulation and Related Enzymes Metabolism inPinellia ternata Suspension Cell Cultures
Yongbo DUAN ; Fang LU ; Tingting CUI ; Fenglan ZHAO ; Jingtong TENG ; Wei SHENG ; Aimin ZHANG ; Jianping XUE
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(1):87-90
Objective To study the effects of abiotic elicitors methyl jasmonate (MeJA) and salicylic acid (SA) on the alkaloids accumulation and related enzymes metabolism inPinellia ternata suspension cell cultures. Methods Using the leaf petioles-derived suspension cell cultures as the study object, the culture duration, concentrations of MeJA and SA were determined to get the optimal alkaloids accumulation, and the activities of metabolic enzymes IMP dehydrogenase and sAMP synthase were also measured.Results A 9-fold of dried biomass and a 3-fold of alkaloids accumulation were observed inP. ternata suspension cell cultures after culture for 21 d. Both MeJA and SA could significantly promote the accumulation of alkaloids inP. ternata suspension cells. 150 μmol/L MeJA enhanced alkaloids content (4.7 mg/gDW) by 3.6 folds in comparison with control group, whereas 50 μmol/L SA showed a 2.5-fold increase. Meanwhile, 100 μmol/L MeJA and 50 μmol/L SA promoted the increase in IMP dehydrogenase activity by 3.0 and 3.7 fold respectively, and 150 μmol/L MeJA and 100 μmol/L SA showed the increase by 2.6 and 4.4 fold respectively.Conclusion Proper adding exogenous MeJA and SA can promote the accumulation of alkaloids inPinellia ternata suspension cell cultures.