Objective:To prepare the recombinant neuron protective protein TAT-Bcl-XL in E.coli expression system and to determine its anti-apoptosis activity.Methods:DNA fragment of TAT-Bcl-XL was obtained by RT-PCR using primers that were specific for Bcl-XL gene. Prokaryotic expression vector(pTBTOPO) was constructed by insertion of TAT-Bcl-XL DNA fragment into pCRT7/CT-TOPO vectors, and the recombinant protein was detected by SDS-PAGE and Western blot analysis using anti-V5 tag epitope antibody as the primary antibody. The recombinant fusion protein was purified by affinity chromatography, and the anti-apoptosis function of the purified protein was determined by flow cytometry.Results:A molecular weight of 30 000 protein was detectable in both SDS-PAGE and Western blot analysis. Immunofluorescent staining showed the fusion protein was distributed in cell plasma after incubation with 200 nmol/L fusion protein. Flow cytometry data indicated that the recombinant protein could enhance cell survival by 40% when 293T cells were treated with 250 ?mol/L zinc chloride as a supplementation in cell culture media.Conclusion:High level expression of the reported neuron protective protein was obtained in E.coli expression system, and the purified recombinant proteins remained its anti-apoptosis activity in our preliminary characterization.

Large-scale clinical trials have demonstrated that metabolic control achieved early in the course of diabetes can substantially help preventing the onset and slowing down the progression of diabetes and the associated microvascular complications. Various strategies incorporating the use of insulin early in the course of the disease have been developed to meet this goal and to compensate for the progression of β-cell failure. We believe that insulin suppresses inflammation and also reduces glucotoxicity and lipotoxicity, and thus exerts a pivotal and beneficial role. Comprehensive metabolic control, especially when instituted earlier, may alter the natural history of diabetic complications. Therefore, physicians are encouraged to take early and intensive interventions with insulin therapy for patients with type 2 diabetes, especially when attempting to induce "remissions" or in patients suboptimally controlled with oral medications.

Sepsis is thought to be systemic inflammatory response syndrome that is activated by invasive infection and characterized by the involvement of inflammation,infection,immunological reaction,coagulation and tissue damage.The imbalance between the pro-and anti-inflammatory response mediated by immunologic disorder plays an important part in the progress of sepsis.Therefore,the immunological mechanisms and changes of Toll-like receptors,neutrophils,dendritic cells and regulatory T cells in sepsis are briefly focused on in the article.

OBJECTIVE To discuss effective methods of nosocomial infection management in operating room to control the nosocomial infection and improve quality of medical care.METHODS To strengthen and improve the environment of operating room,and the management of medical staff,room cleaning and articles,as well as operation classification in there,to establish the effective nosocomial infection management organization in whole hospital,particulerly in operating room,to study technology of hospital infection,and to enhance the consciousness in control nosocomial infection.RESULTS It is very important guarantee that strengthen management of operating room work in hospital could improve the medical safety and prevent the nosocomicl infection.CONCLUSIONS To actualize scientific management in operating room,and control nosocomial infection.

OBJECTIVE To investigate the epidemic situation of nonfermentative bacteria in lower respiratory tract infection and guide rational use of antimicrobials.METHODS API system was applied for bacterial identification.K-B disk diffuse method was used in susceptibility testing.WHONET 5.3 software was used for data analysis.Pseudomonas aeruginosa,Acinetobacter baumannii and Stenotrophomonas maltophilia isolates were included in this study.RESULTS Less than 20% of P.aeruginosa isolates were resistant to amikacin,imipenem or cefoperazone/sulbactam.For A.baumannii,the most active compounds tested were imipenem and cefoperazone/sulbactam with resistance rates of 4.8% and 7.3%,respectively.26.6% And 27.4% of A.baumannii isolates were resistant to piperacillin/tazobactam and ticarcillin/clavulanic acid.The resistance rate of S.maltophilia to cefoperazone/sulbactam,ticarcillin/clavulanic acid and piperacillin/tazobactam was 0,2.7% and 10.8%,respectively.CONCLUSIONS The isolation rate of nonfermenters is increasing in the lower respiratory tract infection,and the ways to improve the cure rate are to avert nosocomial infection and choose antimicrobials properly.

Research on history of TCM should serve for development of TCM today.Researchers in history of prescriptions of Chinese materia medica should think the question about development of prescriptions of Chinese materia medica.Three problems about development of prescriptions of Chinese materia medica are brought forward currently.Firstly what is area of it in science,whether method of treatment and preparation are attached to it .Secondly whether classify of it is accordant to theory of TCM and method of treatment.Thirdly the Pharmacopoeia hold in the prescriptions .The items of Pharmacopoeia couldn’t satisfy clinical needs of TCM in processing and dosage.A standard of prescription should be drawn besides the Pharmacopoeia.

Objective To calculate the cost of inguinal hernia disease .Methods With the inguinal hernia clinical pathway standardized diagnosis and treatment project as the basis , cost was estimated using activity based cos-ting.Results Inguinal hernia treatment project cost is 4 594.96 yuan, higher than the current charges 1 448.36 yuan.Conclusion According to the clinical pathway , cost accounting of disease activity based costing method , can be more scientific to measure out of inguinal hernia disease cost .

Objective To investigate correlational study on the levels of homocysteinin with the severity of coronary artery in patients with coronary atherosclerotic disease.Methods 60 patients with coronary atherosclerotic disease were enrolled in from Janu-ary 2013 to December 2014 in Beijiao hospital affiliated Southern medical university .All patients received coronary artery angiograph and the levels of circulating homocysteinin were determined.According to the levels of circulating homocysteinin, 60 patients were de-vided into coronary atherosclerotic disease group and coronary atherosclerotic disease group with hyperhomocysteinemia ( HHcy) .The levels of homocysteinin and the severity of coronary artery in patients with coronary atherosclerotic disease and correlation between the two group were analyzed.Results Compared with coronary atherosclerotic disease patients,the levels of homocysteinemia and Genisi score were increased in patients with coronary atherosclerotic disease with HHcy (p<0.05).Correlation analysis showed that the lev-els of homocystein is positively correlated with Genisi score (p<0.05).Conclusion HHcy is a important risk for coronary athero-sclerotic disease and positively related with the severity of coronary artery in patients with coronary atherosclerotic disease .

The prevalence of diabetes is increasing dramatically and what is more serious is that the age of onset of diabetes in population is much younger than that of before.The author has carried out a series of research in diabetes,which mainly focused on epidemiology of diabetes mellitus and molecular genetics,early diagnosis and treatment of type 2 diabetes.This article aims to raise the attention of diabetes and promote the individual diagnosis and therapy of diabetes.