The derivatization towards the hydroxyl groups of steroid environmental endocrine disrupting chemicals,such as estrone(E1),17β-estradiol(E2),estriol(E3) and 17α-ethynylestradoil( EE2),with A,O-bis (trimethylsilyl) trifluoroacetamide ( BSTFA ) as derivatization reagent followed by gas chromatography-mass spectrometry(GC-MS) was studied. The effects of the amount of BSTFA,derivatization temperature and time on the derivatization of target compounds,as well as the stability of steroid derivatives,calibration curves,instrumental quantification limits were investigated. The cleavage mechanism of steroid derivatives characteristic fragment ions was also interpreted. The results showed that better derivatization results were obtained when the amount of BSTFA was 25 μL for 100 μL steroids standard mix solution at 0.01 g/L;it is unnecessary to heating in the derivatization,better results were obtained when the steroids were derivatized with BSTFA at room temperature (20℃) for 10 min. The derivatives of target compounds were stable because their relative response factors(RRFs) had no observable change when deposited at -20℃ for 48 h. Under the optimal conditions,good linear correlation was obtained and the instrumental quantification limit was 0. 3μg/L for E1 and E2,while it was 5μg/L for EE2 and E3.

Objective To study the clinical efficacy of massage nursing intervention on children patients with bronchial pneumonia .Methods Totals of 80 children patients with bronchial pneumonia during the period from May 2012 to May 2013 were randomly divided into the control group ( routine nursing ) and the observation group ( massage nursing intervention ) . The clinical efficacy , hospitalization time , cost of hospitalization , and nursing satisfaction in the two groups were observed and compared .Results Compared with the control group, the effective rate was significantly increased (95.0% vs 80.0%), the hospitalization time [(6.5 ±1.5) vs (8.5 ±2.0)d] and cost of hospitalization [(1 900 ±100) vs (2 650 ±150)yuan] were significantly reduced , and the nursing satisfaction degree was obviously increased in the observation group ( great satisfaction:32 vs 24;satisfaction:6 vs 8;dissatisfaction:2 vs 8), and the differences were statistically significant (χ2 =4.11,t=2.21,3.47,Z=4.11,respectively;P<0.05).Conclusions For the children patients with bronchial pneumonia , the massage nursing intervention can significantly increase the clinical efficacy , promote early recovery .