Objective To study and discuss the effect of double hydrogen artesunate on Mcl-1 expression and its inducing effect on cancer cell apoptosis in the patients with cholangiocarcinoma.Methods Bile duct cancer cell lines QBC939 preserved in our hospital from June 2010 to December 2014 were randomly selected and divided into the control group and observation group for conducting experiments.The cells were cultured by using the conventional cultivation and double hydrogen artemisinin culture.Then the Mcl-1 expression and apoptosis of cancer cells were performed the statistical analysis and comparison.Results Statistical comparison showed that the expressions of MCL1-001 and-MCL1 201 at 12,24,48 h in the observation group were significantly higher than those in the control group,the comparison between groups were statistically significant (P<0.05).MCL1-002 expression had little difference between at 12 h and 24 h (P>0.05),but which at 48 h in the observation was significantly higher than that in the control group,the difference was statistically significant(P<0.05).And the mortality rate at 6,12,24,48,72 h in the observation group was significantly higher than that in the control group,the difference was statistically significant (P<0.05).Conclusion Double hydrogen artemisinin has obvious up-regulation effect on Mcl-1,moreover can effectively induces bile duct cancer cell apoptosis.

Medical imaging is evolving from the traditional morphologic examination to a pattern that combines morphologic and functional examinations that can reflect physiochemical changes at cellular and molecular levels. Functional imaging techniques, including CT perfusion imaging, MR diffusion weighted imaging, MR perfusion imaging and MR spectroscopy, have been drawing increasingly more attention in pathological study of the livers, pancreas, kidneys, prostates and lymph nodes, and the prospect is promising. CT & MR functional imaging combined with morphologic examination is helpful for the diagnosis and differential diagnosis of pathological lesions.

Objective To study the changes of imaging features of rats with implanted liver cancer before and after electrochemotherapy (EChT).Methods Immediate cancerous ascites injection method was used to form rats liver cancer model. The imaging features of rats with implanted liver cancer, including tumor size, density, border, enhancement and singal were observed with CT and MRI respectively. Results The characteristic magings showed all these rats bearing single, round tumor in liver seven days later. Low density lesions were seen in pre contrast scans and tumor border intensification were seen on contrast scans. Low signal lesions were found in MRI T 1WI and high signal lesions were revealed on MRI T 2WI. Seven days after EchT, low density lesions were seen in pre contrast scan and non enhanced appearences were seen in contrast scan by CT. High signal and mixed signal lesion were seen in MRI T 1WI and relative low signal lesion were seen in MRI T 2WI.Conclusions Imaging features analysis is useful to assess the therapeutic effect on rats with implanted liver cancer before and after EChT.

Objective To compare the effects of electrochemotherapy (EChT) on implanted liver tumor cells apoptosis of rats.Methods Immediate cancerous ascitis injection method was used for the formation of rats liver tumor model and then followed by rats liver tumor treatment with EChT. Tumor size, necrosis and apoptosis indexes were observed after one week by imaging or pathologic method. The results were compared with those of control group. Results Before EChT, rats liver tumor volume appeared as (100?6)mm 3 and turned to be (125?10)mm 3 one week after EChT, which were significantly smaller than those of the control group [(190?11)mm 3], P

Objective To compare the therapeutic effects of electrochemotherapy (EChT) and radiofrequence ablation (RFA) on rats with implanted liver cancer.Methods Immediate cancerous ascites injection method was utilized for creation of rats liver cancer model and followed by treatment with EchT and RFA respectively. Tumor size, necrosis and apoptosis were observed one week later by imaging and pathology. The results were compared with those of control group. The survival times were observed also. Results Before treatment, all rats average liver tumor volume was (100?6)mm 3. One week after treatment, average tumor volumes in EChT group [(125?10)mm 3] and RFA group [(143?12)mm 3] were significantly smaller than those of control group [(190?11)mm 3], but tumor volume showed significant statistical difference between EChT group and RFA group. Necrosis, apoptosis and survival times in EChT group and RFA group were significantly higher than those of control group.Conclusions EChT or RFA could promote tumor necrosis, induce apoptosis, inhibit tumor growth and prolong the survival time of rats with implanted liver cancer.

ObjectiveTo establish nude mouse tumor models bearing human pancreatic adenocarcinoma SW1990 cells in multiple sites at different time-points and investigate the feasibiilty of multiple tumor-bearing in these models; then the findings and detection rate of 3.0T magnetic resonance image (MRI) in subcutaneous transplanted tumors was analyzed. Methods A total of 6 BALB/C nude mice were randomized into 3 groups (2 mice per group ).At the 1st,8th,15th day,the mice were injected subcutaneously with the suspension of SW1990 cells at left axilla and right axilla and right groin in sequence.Three weeks later,all the bearing-tumor mice were performed with MRI non-contrast enhanced scanning plus Gd-DTPA enhanced scan and the subcutaneous masses were subjected to pathological analysis.ResultsAll the 6 nude mice were alive during the study and obvious mass was observed in every injected site.The tumor size was positively associated with the grwing time.There were 9 tumors which could be de.ted by noncontrast enhanced MRI scanning and one more tumor was detected by contrast enhanced scanning.2 tumors were not detected,the 2 tumors were located at subcutaneous of right groin,with the shortest growing time,and the major axis of the 2 un-detected tumors was less than 5mm.Despite the MRI findings of the transplanted masses similar to that of human pancreatic adenocarcinoma with bleeding,necrosis,they presented the characteristics of a clear rim,with pseudocapeule sign.All the 12 masses were similar with human pancreatic adenocarcinoma under light microscope.ConclusionsIt is feasible to transplant human pancreatic adenocarcinoma cell at three different subcutaneous sites (injected at three different points of time) in the nude mouse,with a minimal survival time of three weeks.However,routine 3.0T MRI cannot detect the early tumors (growing time within 1 week,major axis ＜5 mm).

Objective To improve the diagnosis of germinomas in basal ganglia area by analysing the imaging manifestations.Methods Clinic,pathologic and medical imaging data of 8 cases of germinomas in basal ganglia area confirmed pathologically were analyzed retrospectively.Results 8 cases were all men,the age ranged from 5~13 years old with mean age of 10.5 years.All cases had change of muscle strength on one side of extremity,and one case accompanied by intracranial hypertension,this case was showed the effect of space occupying of the tumor obviously and hydrocephalus on CT and MRI.The findings of CT and MRI showed cystic degeneration in 4 cases,slight edema around the tumor in 2 cases,cortex atrophy of the same side of tumor in 2 cases and calcification in the tumor in 2 cases.All cases were enhanced greatly.The pathologic specimen with rich supply of blood was gray-red,and cystic degeneration was seen in 4 cases.Conclusion CT and MRI features of germinomas in basal ganglia area are quite characteristic,the improvement of diagnosis can be achieved in combination with clinical manifestation.

BACKGROUND:Crowe IV developmental dysplasia of the hip is rarely seen clinical y. However, when treated with routine total hip arthroplasty, severe deformities in the bone and soft tissue can lead to high rate of operation failure and increased occurrence of complications. Total hip arthroplasty in combination with subtrochanteric osteotomy may be an option to resolve this problem.
OBJECTIVE:To retrospectively analyze the clinical outcome and safety of Crowe IV developmental dysplasia of the hip treated by the combination of total hip arthroplasty and subtrochanteric osteotomy in 17 cases.
METHODS:Twenty-one hips (17 cases) of Crowe IV developmental dysplasia of the hip treated by the combination of total hip arthroplasty and subtrochanteric osteotomy were retrospectively analyzed and fol owed for at least 2 years from January 2006 to June 2011. Complications, hip function, and radiological changes were evaluated.
RESULTS AND CONCLUSION:The mean fol ow-up period was (48.0±20.5) months. Harris’s score was increased postoperatively (P<0.05). The main complains were lower limb length discrepancy, difficulty in standing up from squatting, muscle weakness, and soft tissue tightness. One greater trochanter fracture occurred and was fixed by hook plate fixation. There were four femoral shaft splits treated by cerclage. One patient represented with femoral nerve palsy and got partial recovery until the latest fol ow-up. There was no deep infection, dislocation or prosthesis loosening. Crowe IV developmental dysplasia of the hip could be effectively treated by total hip arthroplasty in combination with subtrochanteric osteotomy, which requires high surgical techniques to control the complications. Further fol ow-up is required for long-term results.

Objective To evaluate 99mTc-ciprofloxacin (Infecton) scintigraphy as a method for detecting secondary infections associated with ANP in swine,in comparison with CT.MethodsTwenty-eight healthy swine were randomly assigned to control group (n =6),non-infected ANP (n =6) and infected ANP group( n =16).ANP model was induced by retrograde injection of sodium taurocholate and pancreatic protease mixture into the biliary and pancreatic duct.Two days after ANP induction,swine in infected ANP group were injected with 3 x 108 E.coli into pancreatic tissue,while swine in non-infected ANP group were injected with inactivated E.coli.At 7 d after inoculation,at 0.5,1,2,3,4,and 6 h after intravenous administration of 370 MBq of Infecton,SPECT scan was performed.Then 64-slice spiral CT scan was performed.Then swine were sacrificed,and histopathology examination and bacterial culture of pancreatic tissue were performed.The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of the two methods to detect secondary infections were determined.ResultsThere were no abnormality in the normal pancreas and the bacterial culture was negative.There were pancreatic necrosis in the non-infected ANP group,but the bacterial culture was negative.There were pancreatic necrosis and infection in the infected ANP group and the bacterial culture was positive.The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of the Infecton method were 93.8％ ( 15/16 ),91.7％ ( 11/12 ),92.9％ ( 26/28 ),93.8 ％(15/16) and 91.7％ ( 11/12),whereas these values for CT were 12.5％ (2/16),100.0％ ( 12/12),50.0％(14/28),100.0％ (2/2) and 46.2％ (12/26),respectively.The sensitivity,accuracy,and negative predictive value of the Infecton method were significantly higher than those in CT group (P ＜0.01 ).ConclusionsInfecton scintigraphy may be a better procedure for detecting ANP secondary infections than CT.

Objective To compare the mesothelin expressions in 3 human pancreatic cancer cell lines between in vitro and in vivo and the developing speed among the subcutaneous tumors implanted with the 3 human pancreatic cancer cell lines in nude mice.Methods The human pancreatic cancer cell lines ( SW1990,BxPC3 and PANC1 ) were cultured and then were implanted subcutaneously into left axillas of nude mice.The volumes of these subcutaneous tumors were recorded every week to estimate their developing speed.The mice implanted with SW1990 and BxPC3 cells were observed for three weeks,while the mice implanted with PANC1 cell were observed for five weeks.The Western blot method was used to measure the expressions of mesothelin in the 3 kinds of cells and subcutaneous tumors,while immunohistochemical staining was applied to determine the expressions of mesothelin in 3 kinds of subcutaneous tumors.Results The sequence of quantities of expressions of mesothelin in these cell lines in vitro were BxPC3 ＞ PANC1 ＞ SW1990,and the sequence of quantities of expressions in vivo were SW1990 ＞ BxPC3 ＞ PANC1.One handrued percent of the tumors grew out successfully,and the sequence of speeds of their growth was SW1990 ＞ BxPC3 ＞ PANC1.Conclusions The mesothelin expressions among 3 kinds of pancreatic cancer cell line are different.The developing speeds of tumors originated from different subcutaneous tumors in nude mice are also different,and there is no association between them.