This study is to investigate the transportation of scutellarin in cell and live models and study on mechanism of absorption and transport of scutellarin in mouse liver. The concentration of scutellarin in plasma and liver from control and pretreated groups was determined by high performance liquid chromatography. The uptake of scutellarin was examined in control hepatocytes group, induced hepatocytes group and induced hepatocytes plus pravastatin group. Pravastatin can affect the pharmacokinetics of scutellarin in mouse: CL is decreased while AUC is increased. The scutellarin absorption of hepatocyte induced group was higher than that of control group, but was decreased in the group with pravastatin added. The research showed that there was potential drug interaction between pravastatin and scutellarin. The drugs may compete for oatp2 mediated transport pathway consisted in the uptake of scutellarin in liver.

Objective To compare the value for predicting prognosis between ENETS and AJCC TNM staging system in G-NEC patients.Methods Clinical data of 28 G-NEC cases from January 2005 to September 2015 in Tianjin Medical University General Hospital were analyzed and follow-up data were staged by he ENETS and AJCC TNM staging system.The survival curves were drawn using Kaplan-Meier method.Univariate analysis was performed by Log-rank test.Results Evaluated by ENETS TNM staging system on these 28 G-NEC patients,5 cases (18％) were classified at stage Ⅱ,20 cases (71％) were at stage Ⅲ,3 cases (11％) at stage Ⅳ,and significant difference in survival time was found between the three stages (P ＜ 0.05).By the AJCC TNM staging system,1 case (4％) was classified at stage Ⅱ,24 cases (86％) were at stage Ⅲ,and 3 cases (11％) at stage Ⅳ,and significant difference in survival time was found between three stages.Conclusions AJCC TNM staging system is more valuable for predicting prognosis of G-NEC,while the ENETS TNM staging system is more accurate in evaluating G-NEC biologic behavior.

OBJECTIVE: To explore the changes of parathyroid hormone (PTH) and serum calcium alter total thyroidectomy and summarize the methods to prevent and treat the hypoparathyroidism and hypocalcernia. METHOD: Seventy-three patients with total thyroidectomy in our hospital were divided into three groups according to the scope of the operation. The serum concentrations of PTH and calcium were measured and compared before surgery and after surgery 1 h, 1 d, 3 d and 5 d. RESULT: In three groups, the serum concentrations of PTH and calcium were significantly decreased (P<0. 05) after surgery and low serum PTH and hypocalcemia occured. The severity of low serum PTH and hypocalcemia was as follows: total thyroidectomy with bilateral compartment lymph node dissection (CLND)>total thyroidectomy with unilateral CLND>total thyroidectomy. The differences were statistically significant. The levels of PTH after surgery 1 hour and 1 day were effective to predict hypocalcemia. CONCLUSION Total thyroidectomy can affect the parathyroid function. The greater the scope of surgery, the higher the possibility of postoperative hypoparathyroidism. The reasonable operative procedures and more protection of parathyroid during operation can reduce the incidence of hypoparathyroidism. The level of postoperative PTH can be used as a predictive index of hypocalcemia.
Calcium ; blood ; Humans ; Hypocalcemia ; blood ; Hypoparathyroidism ; blood ; surgery ; Incidence ; Lymph Node Excision ; Parathyroid Glands ; physiology ; Parathyroid Hormone ; blood ; Postoperative Period ; Thyroidectomy

ObjectiveTo observe the clinical efficacy of electroacupuncture plus herbal-partitioned moxibustion in treating temporomandibular joint disorder.MethodNinety patientswith temporomandibular joint disorder were randomized into a treatment group of 45 cases and a control group of 45 cases. The treatment group was intervened by electroacupuncture plus herbal-partitioned moxibustion, while the control group was by medication. The therapeutic efficacy was evaluated after 3 treatment courses by a specially-assigned person.ResultThe recovery rate and total effective rate were respectively 64.4% and 97.8% in the treatment group versus 37.8% and 82.2% in the control group, andthe total effective rate and recovery rate in the treatment group were significantly higher than that in the control group (P<0.05); regarding the comparison of the efficacy and treatment duration of the recovered subjects, the recovery rates at the end of the 1st course, 2nd course and 3rd course in the treatment group were all markedly higher than that in the control group (P<0.05), indicating that electroacupuncture plus herbal-partitioned moxibustion can shorten the period of treatment for temporomandibular joint disorder. The therapeutic efficacy of the treatment group was significantly superior to that of the control group(P<0.05).ConclusionElectroacupuncture plus herbal-partitioned moxibustion can produce a significant efficacy in treating temporomandibular joint disorder.

Objective To compare the long-term efficacy of the traditional open surgery (OSP) with the thoracic endovascular aneurysm repair (TEVAR) in the treatment of patients with the complicated Stanford B aortic dissection. Methods The retrospective analysis of the clinical data was conducted in 118 patients (OSP 45 vs. TEVAR 73) with the complicated type B aortic dissection from January 2004 to January 2015. The long-term survival rate was compared between the two groups and the influencing factors of the postoperative survival was analyzed by using the Cox-regression analysis. Results Significant differences in age, sex and operation time were observed between the two groups (P < 0.05, P < 0.01); No significant difference in the benefit of long-term survival was found between the two groups (P > 0.05). The refractory hypertension and preoperative aortic overall diameter > 55 mm were the risk factors for the long-term survival (P = 0.021, OR = 11.1, 95%CI:1.428 ~ 86.372; P = 0.001, OR = 4.5, 95%CI: 1.842 ~ 11.346). Conclusions Compared with OSP, no obvious advantage of TEVAR was shown in the ten-year overall cumulative survival. In view of the influence factors of refractory hypertension and the aortic diameter for the long-term survival , we should pay attention to the blood pressure control and the performance of endovascular repair technology whether pre- or post-operation.

Objective To observe early histopathological changes in explosive wounds at canine limbs after seawater inmersion.Methods Forty adult dogs,weighing 10 to 15 kg each,were assigned into 2 equal groups.The left hind limb of each dog in both groups was injured by a man-made explosion.The explosive wound was first washed by normal sodium.The 20 injured limbs in the experimental group (EG)were immersed in seawater for one hour while those in the control group (CG) were only exposed naturally for one hour without seawater immersion.All the wounds were covered with sterile dressing without suture.The pus and soft tissue at the wound were taken for pathological examination,bacterial culture and drug sensitivity test on day 3.The time of wound healing was recorded.Tissue sections were taken from the wounds for pathological examination at 4 and 8 weeks.Results Fifteen wounds (75％) were infected in the EG,significantly more than the 8 wounds infected (40％) in the CG( P ＜ 0.05).The wounds healed in a mean time of 38.4 days in the EG,significantly longer than the mean time for wound healing (23.1 days) in the CG ( P ＜ 0.05).In the EG,Vibrio infection caused more serious tissue necrosis and inflammatory reaction than Bacillus and coccus did.At 4 and 8 weeks,tissue necrosis and inflammatory reaction in the EG were worse than those in the CG.Conclusion Seawater immersion can lead to increased infective tissue necrosis and inflammatory reaction in an explosive wound,as well as longer time for wound healing.

The hemodynamic mechanism has been considered to be the main factor related to the cerebral aneurismal growth and rupture. Virtual numerical research has become an important method for the study of cerebral aneurismal hemodynamics, which lead to a deep insight into the field of hemodynamic mechanism.We reviewed the correlative researches in this field during recent years and made a summarization concerning about, the relationship between intra-aneurysmal hemodynamics like velocity,wall tangential stress and pressure field under variable conditions and the biological behaviors such as growth and rupture of aneurysms; and further discussion on the topic of clinical application.

Objective To establish the quality control method for Bushenbanlong Tablet. Methods The content of astragaloside was determined by HPLC-ELSD. Results The linearity range was 1.44～4.32 ?g with a correlation coefficient r=0.999 9, and the average recovery was 99.69% with RSD=1.16%. Conclusion The method was simple, convient, quick and sensitive. It provide an effective method for quality control of Bushenbanlong Tablet.

Objective To evaluate the feasibility, safety and efficacy of selective portal vein embolization (SPVE) in treatment of liver tumor in rats and to provide the groundwork for its future clinical applications. Methods 24 healthy rats underwent the embolization. Pre and post SPVE portogram and liver chemical profile were obtained. Four rats were sacrificed at 10 min, 7,14, 21 and 28 days respectively following follow up portography. The liver, heart, lungs and kidneys were examined macroscopically and microscopically. Fifteen rats implanted with Walker 256 tumor sized from 3 to 10 mm in liver were scanned with MRI and portography pre SPVE taken. Post SPVE 3 rats were examined with MRI for each group at the same interval as above and the lives were examined microscopically. Results (1) The blood flow to the target portal branches were immediately halted after SPVE. These vessels remained occluded without collateral formation up to 28 days. (2) The liver indexes and BUN level increased after embolization, but returned to normal within 21 d. Macroscopic and microscopic changes were not found in the heart, lungs or kidneys. (3) In the healthy rats, the affected segment was atrophic and the remaining liver underwent compensatory hypertrophy. Histologic examination revealed that the targeted portal veins were coagulated, the endothelium were degenerated and the local hepatocytes were necrotic after embolization. (4) In the rats with implanted liver tumor, the affected segment including the tumor was necrotic and atrophic. The tumors were completely necrotic, and no viable tumor cell was seen under microscope in 12 among the 15 rats. Three tumors 10 mm in diameter were not completely necrotic. Part of tumor cells were still alive and infiltrated into the surrounding liver. Conclusion SPVE with ethanol is effective in the treatment of small liver tumor in rats. However,in case of bigger tumors involving several segments, SPVE should be combined with other treatment.