Objective To observe the correlation of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) appearances and blood stasis syndrome in breast cancer.Methods The clinical data of 100 patients with breast cancer confirmed by operation and pathology were retrospectively reviewed.70 cases without blood stasis syndrome,30 cases with blood stasis syndrome.All the cases underwent MRI using PHILIPS Achieva 1.5T magnetic resonance scanner before surgery.Results In no blood stasis group,76.67％ inhomogeneous enhancement,edge enhancement 43.33％,spiculation proportion 50.00％,which were significantly lower than those in the blood stasis group (88.57％,75.71％,83.33％).The mass ratio of the shape between the two groups had no significant difference (P ＞ 0.05).No blood stasis group and stagnation of blood group TIC curve was mainly type Ⅳ,type Ⅲ curve ratio of no blood stasis group (3.33％) was significantly lower than blood stasis group(12.86％) (P ＜0.01).In blood stasis group,Emax/1 and tumor size were higher than without blood stasis syndrome group.the percentage of V per minute outflow of blood stasis group was lower than without blood stasis syndrome group.Conclusion The clinician should make a preliminary evaluation of the prognosis before operation,in order to timely and reasonably choose the method of integrated traditional Chinese and Western medicine.

Objective To observe apoptotic cells and caspase-3-positive cells in ipsilateral neonatal hypoxic-isch?emia encephalopathy (NHIE) model in mice. Methods CD1 mice of age 7 days (n=30) were randomly divided into two groups: sham group (n=9) and model group (n=21). NHIE model was induced by right common carotid artery ligation fol?lowed by 8%oxygen hypoxia for 100 min. TTC staining was used to determine area of brain infarction. DAPI staining was used to detect pathological change in brains. TUNEL assay was used to detect apoptotic cells and fluorescence immunohisto?chemistry was used to detect caspase-3 expression in the ipsilateral brain. Results No infarct was detected in sham group. Cells were densely and orderly arranged in brain. TUNEL-positive cells (18.57±4.98) and caspase-3-positive cells (9.17± 2.14) in the ipsilateral brain were both less than those in the ipsilateral brain of mice in model group (209.57±41.27) and (63.33±16.22) respectively. Mice in model group presented infarct in the right hemisphere with more dead cells and wider in?terstitial space compared with sham group. Conclusion Brain injury in NHIE model might be related to the increasing cas?pase-3 expression thus leads to apoptosis.

Objective To evaluate the curative effect of selective window decompression surgery for over 80-year-old patients with mul-tiple segment lumbar spinal stenosis. Methods The patients received the operation through posterior median approach. The location of le-sions were determined according to patients' symptom,physical examination and imaging examination,the selective window decompression sur-gery was performed in patients,JOA scoring method was used to evaluating the effect of surgery. Results In the recent follow-up of first 6 months after surgery,the response rate of 52 patients with follow-up was 100%. During the mid-term follow-up after 24~36 months,the re-sponse rate of 47 patients with follow-up is 90. 4%,the average follow-up time was 30. 4 months. The preoperative JOA score and postopera-tive score were (6. 93 +0. 52) and (11. 98 +0. 72) respectively,of which 40 cases achieved optimal standard (76. 9%),10 cases (19. 2%) achieved a good standard,2 cases reached the standard (3. 9%),the excellent rate was 96. 1%. The JOA score of mid-term fol-low-up was (12. 06 +0. 46),of which 36 cases reached optimal standard (76. 6%),10 cases achieved a good standard (21. 3%), but standard was in 1 case (2. 1%),the excellent rate was 97. 9%. The difference of JOA scores was statistically significant (P<0. 01). Con-clusion Selective windowing decompression has the advantages of small trauma,short recovery time and satisfied curative effect,which is suitable for elderly patients with lumbar spinal stenosis.

Objective To search a new,simple,minimally invasive,safety and effective method to treat the cervical disc herniation.Methods 210 patients with cervical disc herniation received percutaneous nucleoplasty were evaluated.Results They were followed prospectively from two weeks to two months,fifty-nine patients improved significantly,the general clinical effective rate was 96.2%.No complication occurred.Conclusion The percutaneous nucleoplasty is an effective,rapid suited to outpatient,minimally invasive and safe procedure for cervical disc heriation.

Objective To retrospectively analyze the indication of endosseous dental implant,evaluate the effectiveness of clinical application of endosseous dental implant for tooth loss,and study the possible factors which may affect the success rate of implant denture.Methods One hundred and fifty-seven endosseous dental implants in 94 patients were followed up for 4 months to 8 years.The indications of endosseous dental implant were studied based on the changes in the distribution of dental implants in jawbone.The success rate of osseointegration and restoration of the endosseous dental implant were evaluated by checking the soft tissue around the implants,the stability of the implants,the bone resorption around the implants,and the degree of stability against rotation of the implants.Results The rate of performing endosseous dental implant for restoration of full-mouth anodontia was 7.6%,the rate of endosseous dental implant used in the free end anodontia was 25.5%,and it was 66.9% in non-free end anodontia.The success rate of osseointegration in the endosseous dental implant was 98.1%,and 5 years after prosthesis the success ratio of the endosseous dental implant was 93.8%.The success ratio of the endosseous dental implants which the primary stability(anti-rotation torsion)

Background Benign lymphoepithelial lesion of lacrimal gland is not a common orbital disease in clinic,which mainly presented as symmetrical and painless enlargement of bilateral lacrimal glands.However,the etiology and pathogenesis of this disease is still unclear now.Objective This study was to screen the differentially expressed genes between benign lymphoid epithelial lesion of lacrimal gland and orbital cavernous hemangioma and explore the pathogenesis of benign lymphoepithelial lesion of lacrimal gland at the molecular level.Methods Nine patients diagnosed as benign lymphoepithelial lesion of lacrimal gland in Beijing Tongren Hospital,Capital Medical University were enrolled from September 2010 to April 2013,and nine patients with orbital cavernous hemangioma served as control group.The intraorbital tissue was collected during surgery.Whole-genome gene expression microarray was used to detect the expressed genes,and limma algorithm was used to analyze the differentially expressed genes between the benign lymphoepithelial lesion of lacrimal gland and the orbital cavernous hemangioma.Real-time PCR was used to verify differentially expressed genes,Fisher method and gene ontology (GO) functional analysis were performed to realize function and signaling pathways analysis.This study complied with Helsinki Declaration and the protocol was aproved by Institutional Review Board of Beijing Tongren Hospital,and informed consent was obtained.Results Total 5 260 differentially expressed genes were screened between benign lymphoepithelial lesion of lacrimal gland and orbital cavernous hemangioma.The Fisher function and signaling pathways analysis showed that 109 GO terms were significantly upregulated and 101 GO terms were significantly downregulated,and 32 relevant signaling pathways were significantly upregulated and 25 signaling pathways were significantly downregulated in the benign lymphoepithelial lesion of lacrimal gland.GO analysis showed that the expression enrichment of complement receptormediated signaling pathway was high,then following the upregulation of T cell and B cell signaling pathways and downregulation of mitogen-activated protein kinase (MAPK) and transforming growth factor-β (TGF-β) signaling pathways.Real-time PCR results showed that the expressions of TIPRL,TLR7 and TLR10 genes were significantly higher in the benign lymphoepithelial lesion of lacrimal gland than that in the orbital cavernous hemangioma,with significant differences between the two diseases (Z =-2.03,-2.32,-2.32;all at P＜0.05),which was consistent with the microarray data.Conclusions Gene expression profiles are significantly different between benign lymphoepithelial lesion of lacrimal gland and orbital cavernous hemangioma.Those differentially expressed genes play roles in the upregulation of T cell and B cell signaling pathways,downregulation of MAPK and TGF-β signaling pathways and the change of complement system.It is implied that a comprehensive effect of various genes and pathways participates in the pathogenesis of benign lymphoepithelial lesion of lacrimal gland.

Objective To observe the expressions of NeuN and pCaMKⅡα in brain and test the spacial learning and memory in neonatal hypoxic?ischemia encephalopathy ( HIE ) model mice. Methods 7d ICR mice were randomly divided into sham group( n=19) and model group( n=23). HIE model was induced by right common carotid artery ligation followed by 8% oxygen hypoxia for 100 min. DAPI staining was used to examine brain pathological change,immunofluorescent staining was used to examine the expression of NeuN and pCaMKⅡα in the ipsilateral brain,and Morris water maze was used to test the spa?cial learning and memory. Results Mice in sham group showed that brain cells were arranged in a dense and orderly manner,the number of NeuN?positive cells and pCaMKⅡα?positive cells were (106.50±20.07), (87.17±16.55) respectively in the brain,and the escape latency was short. Compared with mice in sham group,mice in model group showed more cells loss,less NeuN?positive cells(19.17±3.60) and less pCaMKⅡα?positive cells(13.33±3.62) in the ipsilateral hemisphere,and longer escape latency(P<0.01). Conclu-sion The spacial learning and memory are impaired in hypoxia ischemia,which may be related to the de?creasing expression of pCaMKⅡα in neurons in ipsilateral brain.

A study on the reform of the evaluation method for college students' innovative experiment was carried out on account of the major problems existed in the present evaluation system.A set of evaluation indicators emphasizing process evaluation was constructed based on the theory of developmental evaluation.Also the performance appraisal methods for all indicators were determined.Practice suggested that the evaluation method based on developmental evaluation was able to promote the ability of scientific research and innovation of college students.

Spinal cord injury (SCI) is a kind of severe central nervous system trauma causing motion and/or sensation dysfunction. MicroRNAs are short non-coding RNAs that suppress the translation of target genes, and play an important role in gene regulation involved in spinal cord development and SCI, which constitute novel targets for therapeutic intervention to promote repair and regeneration.

Objective To observe the antioxidative effect of Ginkgo biloba extract (EGb) on motor function after spinal cord injury (SCI) in rats. Methods 36 healthy adult Sprague-Dawley rats were divided randomly into EGb group (group A), methylprednisolone (MP) group (group B) and control group (group C) with 12 rats in each group. SCI model was made by Allen's mode (10 g×25 mm) at the level of the 9th thoracic vertebra (T9). Group A was given 40 mg/kg EGb daily by intraperitoneal injection, while group B was given 30 mg/kg MP at the first injection and then 5.4 mg/kg every 6 hours for 4 times. Group C received an equal volume of normal saline daily. On the 1st, 3rd and 7th days after SCI, 4 rats were selected randomly from each group. The motor function were assessed by Basso-Beattie-Bresnahan scale (BBB). The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum were determined. Results At every time point after surgery, the BBB scores were significantly higher in groups A and B than in group C (P<0.05). Compared to group C,the activity of SOD increased and the content of MDA decreased significantly in groups A and B on the 3rd and 7th days after SCI (P<0.05).Conclusion EGb may reduce the level of oxidative stress and promote the recovery of the motor function in rats after SCI.