Objective To investigate the relationship between endothelin-1(ET-1)and NO in cerebral spinal fluid(CSF) and cerebral vasospasm(CVS) following experimental subarachnoid hemorrhage(SAH).Methods An experimental CVS model was achieved by twice injections of fresh autologous arterial blood into the cisterna magna of each animal. The contents of ET-1 and NO in CSF were measured by radioimmunological analysis and activated cadmium reduction method respectively.The degrees of CVS were analyzed through cerebral angiography(%reduction of basilar artery diamiter,%RBAD). Results The contents of ET-1 in CSF increased significantly than that before injection after SAH(P

Objective To investigate the effect of lumbar subarachnoid space continuous drainage on cerebral vasospasm(CVS) prevention and treatment following experimental subarachnoid hemorrhage(SAH), and further explore the mechanism of CVS prevention and treatment.Methods An experimental SAH was achieved by twice injections of fresh autologous arterial blood into cisterna magna of each dog. The dogs were randomly divided into drainage group and control group and it was just after the second injection of fresh blood that the drainage group began to drain blood cerebral spinal fluid(CSF).The contents of red blood cell(RBC), endothelin-1(ET-1) and nitric oxygen(NO) in CSF were measured respectively. The degree of CVS was analyzed through angiography (%reduction of basilar artery diamiter,%RBAD). The angiographic results of CVS were analyzed and the changes of RBC, ET-1 and NO in CSF at different stages were compared between two groups. Results The drainage group had fewer cases of CVS and the severity of CVS was more slight compared with control group.Drainage group had a significantly higher cleaning rate of RBC.In drainage group,the content of ET-1 was lower and the content of NO was higher significantly than control group.Conclusions Through cleaning the vasogenic substances in subarachnoid space,lumbar subarachnoid space continuous drainage can change the levels of ET-1 and NO in CSF and may prevent and reverse CVS following.

Objective In this study,the authers investigate the effect of lumbar arachnoid space continuous drainage to prevent and reverse cerebral vasospasm (CVS) angiographically following experimental subarachnoid hemorrhage (SAH).Methods SAH model was achieved by cisternal magna twice injections of autolugous blood.Thirty adult mongrel dogs presented with SAH were randomly divided into five groups: early-drainage group,later-drainage group,early-puncture group,later-puncture group and control group. The lumbar puncture two groups started to puncture and exclude blood cerebral spinal fluid(CSF) 2 hours later following the second injection and on day 5 respectively,while the drainage two groups started to drain at the same time.The degrees of CVS were analyzed through cerebral angiography(%reduction of basilar artery diamiter,%RBAD). Results ①Compared with early puncture group and control group,early drainage group had less cases of CVS and the degrees of CVS were more slight which relieved within shorter period than other two groups.②Compared with later puncture group and control group,later drainage group had less cases of CVS and the period of CVS was shorter.③Compared with later drainage group,there were less cases of CVS and the degrees of CVS were more slight in early drainage group.Conclusions Lumbar subarachnoid space continuous drainage may prevent and reverse the CVS following SAH.Early lumbar subarachnoid space continuous drainage should be presented with better consequence.

To observe the effect of aerobic exercise on the body shape,cardiovascular,physiology and biochemical indexes of elderly women,we trained 76 old women (volunteers,mean age 58 years old) from urban area of Yulin from 14th July 2005 to 14th January 2006,60 minutes every time,3 times a week. The heart rate during exercise was controlled within 110-125 times per minute. The changes in their body shape (body mass and waistline),cardiovascular function (resting heart rate and blood pressure) and biochemical indexes before and after exercise were observed. The results showed that after 24 weeks of aerobic exercise,the body mass and waistline of the subjects were decreased compared with that before exercise (P

Objective Investigation of the clearance rate of RBC in cerebral spinal fluid(CSF)after lumber subarachnoid space continuous drainage following subarachnoid hemorrhage(SAH)and the relationship with cerebral vasospasm(CVS) were carried out. Methods An experimental SAH was achieved in dogs by twice injecting fresh autologous arterial blood into cisterna magna of each animal.RBC counts in CSF were measured in the drainage group, puncture group and control group respectively.The degrees of CVS were analyzed through cerebral angiography(%reduction of basilar artery diameter,%RBAD). Results Comparing with puncture group and control group, the drainage group showed a significantly higher clearance rate of RBC with lesser occurrance and degree of CVS. Conclusions RBC and its degeneratives in CSF may be involed in the process of CVS following experimental SAH. Continuous lumber subarachnoid space drainage may prevent CVS.

Objective:To observe the effects of toadpoison and arsenic on the ultrastructure of dental pulp,Methods :Dental pulp of 36 teeth in 3 dogs was exposed by operation and then direct dressing with toadpoison paste or arsenic paste was performed. 1,2,6 and 24 hours after operation ,the pulps were processed and examinedunder a trasmission electron microscope (JEM-2000EX). Results:In toadpoison treated group ,the deformityof the basernembrane of pulp nerve ,swelling of metochondrion,degeneration of nerve axite were observed 1 h after dressing,and were more obvious in 2, 6 and 24 h. Damage of capillary and pulp cells were alsoobserved.In arsenic treated group ,swelling and break of capillaries ,deformity and degeneration of mitochondrion and endoplasmic reticulum in endoepithelial cells were observed,and were more obvious following time lasting. Damages of pulp nerve and pulp cells were also observed. Conclusion:Toadpoison and arsenic are toxic to pulp tissues. But the angiotoxicity of arsenic is stronger than that of toadpoison,the neurotoxicity and protoplasmic toxicity of toadpoison are stronger than those of arsenic.

BACKGROUND:Pathological examination and MRI have been widely used in clinic, but their combination is rarely reported in discrimination of early spine infections.
OBJECTIVE:To determine the accuracy of pathology and MRI for discrimination between early pyogenic spondylitis and brucella spondylitis.
METHODS:Twenty-two patients with pyogenic spondylitis and 20 patients with brucella spondylitis who had CT-guided percutaneous biopsy and MRI of the spine were retrospectively reviewed. Pathological observations included structure and activity of bone lesions, tissue cells and their main components;MRI observations included signal and sign changes at lesion sites. Statistical analysis was performed with the chi-square test.
RESULTS AND CONCLUSION:The patients with pyogenic spondylitis had a significantly higher incidence of pathological and MRI findings as fol ows (P<0.05):neutrophil infiltration;intervertebral disc abnormal signal, location of vertebral body lesions anterior+posterior, obviously shape change in the vertebral body, paraspinal abnormal signal, presence of intraosseous or paraspinal abscess. Pathological and MRI examination was accurate for early differentiation of pyogenic spondylitis from brucella spondylitis.

Objective To calibrate the dose of 12MeV high-energy electron for Siemens MD7745 medical linear accelerator.Methods The international protocols,contents and technical standards were expatiated.Some existing problems in dose calibration of high-energy electron were analyzed.Results The working precision met the requirement of quality assurance in radiotherapy by WHO.Conclusion In order to improve the therapeutic effect,the dose must be calibrated,and the error in dose calibration of high-energy electron should be reduced to the lowest.

Objective: To investigate the hyponatremia incidence in patients with ruptured intracranial aneurysms and the correlation with Fisher grading and cerebral vasospasm. Methods: Totally 150 patients with ruptured intracranial aneurysms from March 1998 to May 2001 were divided into anterior communicating artery (ACoA) group,posterior communicating artery (PCoA) group and middle carotid artery(MCA) group according to the sites of aneurysms.The patients were treated with GDCs interventionally and the incidences of hyponatremia were analyzed. Results: The incidences of hyponatremia in 3 groups were 48%(32/67),15%(8/52) and 16%(5/31),respectively; The incidences of severe hyponatremia of 3 groups were 30%(20/67),6%(3/52) and 6%(2/31), respectively. In ACoA group, the incidences of hyponatremia in Fisher grade Ⅲ-Ⅳ and Ⅰ-Ⅱ were 67%(20/30) and 32%(12/37) and the incidences with and without vasospasm were 71%(24/34) and 24%(8/33), respectively. Conclusion: The ACoA group has a significantly higher incidence of common and severe hyponatremia than the other groups. In ACoA group,hyponatremia is significantly more often in Fisher grade Ⅲ-Ⅳ cases and those with vasospasm.

In this study, the effect of prophylactic anti-inflammation on the development of smoke-induced emphysema was investigated. Young male guinea-pigs aged 1.5-2 months (weighing 198.3+/-26.9 g) were randomly divided into 4 groups: group A (cigarette smoke exposure only), group B (cigarette smoke exposure plus pentoxifylline-rich (PTX, 10 mg/d) forage feeding), group C (cigarette smoke exposure plus intermittent cortical steroid injection (Triamcinolone acetonide, 3 mg, i.m., every three weeks) and control group (group D: animals with sham smoke exposure, raised under the same conditions). Animals in group A, B and C were exposed to smoke of cigarettes for 1 to 1.5 h twice a day, 5 days a week. All animals were killed at the 16th week and followed by morphometrical analysis of the midsagittal sectioned lung slices. Smoke exposure of 16 weeks resulted in visible emphysematous development in Group A but not in Group B and C. It was evidenced by the indicator of air-space size, mean linear intercept (Lm): 120.6+/-16.0 microm in Group A; 89.8+/-9.2 microm in Group B and 102.4+/-17.7 microm in Group C. The average Lm in either group B or group C was shorter than that in Group A (ANOVA and Newman-Keuls test, F=8.80, P=0.0002) but comparable to that (94.8+/-13.2 microm) in group D (P>0.05). It is concluded that long-term prophylactic anti-inflammation inhibits pulmonary emphysema induced by cigarette smoking in the guinea pigs.
Anti-Inflammatory Agents/*pharmacology ; Pentoxifylline/pharmacology ; Pulmonary Emphysema/etiology ; Pulmonary Emphysema/pathology ; Pulmonary Emphysema/*prevention & control ; Random Allocation ; Smoking/*adverse effects ; Triamcinolone Acetonide/*pharmacology