Objective:To investigate the effects of thyroid motilin(MTL) on migrating motor complex(MMC) in fasting rats.Methods: The distribution and quantitative expression of motilin in the thyroids were detected by immunohistochemistry and radioimmunoassay.We also observed the effect of thyriod motilin on the MMC by recording the gastrointestinal motility in conscious rats.Results: Tail vein injection of MTL increased MMC in antrum and duodenum in a dose-dependent manner,as manifested by increased contraction frequency and amplitude(P

Objective To investigate the influence of bifidobacterium on inflammatory response of brain traumatic injury patients.Methods According to the sequence of patients'admission time,a total of 60 patients with traumatic brain injury were randomly divided into the research group and the control group,30 cases in each group.The research group was fed intestinal nutrient solution combined with bifidobacterium,and 1 × 108cfu/d bacteria every day.The control group was skimpily given enteral nutrient solution.The WBC counts,C-reactive protein (CRP) and interleukin-6 (IL-6),GCS score and infection status,ICU length of stay and 30 days mortality in the 1 st,5th,10th,15th day of enteral nutrition were recorded by health care professionals.Results The leukocyte count,CRP and inflammatory factors IL-6 of the research group were significantly lower than those in the control group in the 15th day of enteral nutrition[(8.02 ± 2.47) × 109/L vs (10.54 ± 3.98) × 109/L,(28.02 ± 9.43) mg/L vs (70.54 ± 13.92) mg/L,(61.02 ± 22.43) ng/L vs (83.52 ± 14.94) ng/L; t =5.474,6.231,9.343,P =0.029,0.049,0.001] ;The GCS score of the two groups gradually increased,and which of the first 10 days had no significant differ ence between the two groups.In the 15th day of enteral nutrition,GCS score of the research group was significantly higher than the control group [(12.02 ± 2.12) point vs (10.12 ± 4.94) point,t =9.744,P =0.000].The incidence rate of infection of research group was significantly lower than that of the control group,but ICU length of stay shortened (16/30 vs 27/30,x2 =9.610,P =0.002).No significant difference was observed in 30-day mortality between the two groups.Conclusion Adding an appropriate amount of probiotics in enteral nutrition on regular basis can significantly reduce the body's inflammatory response and the incidence of infection in patients,shorten hospitalization days.

Objective:To study the changes of endothelin-1(ET-1) and malondialdehyde (MDA) in Kawasaki disease (KD) and to probe the relationship between the changes of ET-1, MDA and coronary artery pathological changes. Methods: KD group included 42 patients ( male 25, female 17), with a mean age of (3.25?0.75) years old; control group included 30 normal children (male 19, female 11), with a mean age of (3.58?0.55) years old. Serum contents of ET-1 and MDA were measured by radioimmunology and modified thiabarbituricaid(TBA) method in 2 groups, and the inner diameters of coronary artery and aorta were measured by color echocardiography. KD group was subdivided into dilated-coronary artery group and undilated group for comparison.Results: Serum contents of ET-1 and MDA in KD group were apparently higher than those in control group (-[76.63?18.36-] vs -[41.55?16.68-] pg/ml and -[3.18?0.60-] vs -[1.52?0.24-] ?mol/L,respectively;P

OBJECTIVE:To optimize the basic formula of Compound Salicylic Acid and Chloramphenicol Gel with orthogonal design test.METHODS:The closage of CMC-Na、glycerin and ethanol were taken as the review object with the oligation grade as the evaluation index to conduct L9(33) orthogonal test and the contents of salicyclic acid and the chloramphenicol were determined by HPLC. RESULTS: The optimized basic formula was as follows: sodium carboxymethylcellulose 3 g, glycerine 10 g, and alcohol 35 mL.The average recovery of Salicylic Acid and Chloramphenicol were 99.1% and 101.6%,the RSD were 0.8% and 1.3%.CONCLUSION: The basic formula optimized with orthogonal test is proved to be stable and serve as theoretic basis for the industrialized production of this preparation.

Objective To observe the effects of Chinese herbal compounds (alkaloid of Coptidis Rhizoma 40%, total saponin of panax notoginseng 35%, and polysaccharide of Ophiopogonis Radix 25%) on levels of blood glucose, fructosamine, and insulin of diabetic rats, and explore mechanism of action.Methods Ten rats were randomly chosen as blank control group, and other rats were used to establish diabetic rat models by STZ intraperitoneal injection. Successfully molding rats were randomly divided into Chinese herbal compounds high, medium, low dose groups, metformin group, and model group, 10 rats in each group. Each rat received gavage for continuous 4 weeks. Then rat blood was collected by cutting head and taking caudal vein. FBG was detected by glucose oxidase method;insulin level was detected by radio-immunity double antibody method;fructosamine content was detected by fructosamine method.Results Compared with the blank control group, model group the levels of FBG, froctosamine content increased, while fasting insulin level were lower after 4 weeks'medication. Compared with the model group, the levels of FBG, froctosamine content in Chinese herbal compounds high and medium dose groups and metformin group were lower, while fasting insulin level increased after 4 weeks'medication (P<0.05).Conclusion TCM herbal compounds can increased the basic insulin level by decreasing the contents of FBG and fructosamine.

Objective To investigate the correlation between uric acid level and macrovascular disease in type 2 diabetic patients.Methods Sixty type 2 diabetic patients with lower limb atherosclerosis of carotid artery were randomly selected in study group who hospitalized in the First Hospital of Zibo from Mar.to Feb.2012.Sixty type 2 diabetes mellitus(T2DM) without carotid and lower limb athemsclerosis were served as control group.The blood pressure,blood lipid,blood glucose and other biochemical indexes,including blood uric acid,serum insulin (FNS),fasting blood glucose (FPG),apolipoprotein a (LP (a)),apolipoprotein A1,B (APO-A1,APO-B),glycosylated hemoglobin (HbA1c),high density lipoprotein cholesterol (HDL-C),low density lipopmtein cholesterol (LDL-C),triacylglycerol (TG) and total cholesterol (TC) were measured and determined.Results There was no significant difference in terms of blood pressure,blood lipid levels,APO-A1,APO-B,HbA1C,FNS and FPG in study group patiems (P ＞ 0.05).The level LP(a) in study group was (0.4 ± 0.2) g/L,significantly higher than that in control group ((0.2 ± 0.2) g/L; t =3.842,P ＜ 0.01).The blood uric acid level in study group was (362.3 ± 112.8)mmol/L,significantly higher than that of the control group((284.8 ±68.6)mmol/L;t =3.188,P＜0.01).Conclusion Uric acid and LP(a) are involved in the oocurrence and development of athemsclemsis,which is close related to the development of type 2 diabetic macmangiopathy.Therefore,in the process of preventing type 2 diabetes with macroangiopathy,we should pay attention to uric acid and LP (a) of the patient beside effective control of blood glucose,blood pressure,blood lipid level.

Objective To study the mechanism of the protective effect of lycium barbarum polysaccharide on light aging resistance rats by using the metabolic profile and metabolic target analysis technique.Methods Ultraviolet irradiation induced Wistar rats were induced to produce skin photo aging model and 24 rats were randomly divided into three groups, including control goup, model group, Lycium barbarum polysaccharide group (LBP). After modeling for 24 hours, LBP group was conducted with Lycium barbarum polysaccharide solution of 10 mg/kg. Control group and model group were given same volume of stroke-physiological saline solution for 14 days. The biochemical indexes such as rat serum antioxidant activity of related substances and MDA were measured in model group and drug group; the urine metabolomics study was also investigated for the mechanism of lycium barbarum polysaccharide against light aging band.Results Compared with the model group, the LBP group rats total superoxide dismutase activity (301.51 ± 42.56 U/mgvs.93.41 ± 56.31 U/mg), hydroxyproline (8.91 ± 5.78μg/mgvs.4.74 ± 1.54μg/mg) content significantly increased (P<0.05), but the malondialdehyde (8.54 ± 6.41 nmol/mgvs.21.31 ± 6.58 nmol/mg) decreased without any statistics difference (P<0.05). The urine metabonomics results showed that LBP could regulate the skin photoaging of multiple metabolic pathways and key metabolic enzymes in the process, such as peanut four acid, tyrosine, taurine, citric acid and hippuric acid, L-cysteine, inositol, threonine etc.Conclusions In the process of skin photoaging in rats, multiple metabolic pathways in vivo were disordered, and Lycium barbarum polysaccharide could play a protective role by regulating the key metabolic enzymes in the network.

Objective To describe the diagnostic approach and clinical outcomes of fetal intraabdominal extralobar pulmonary sequestration (IEPS).Methods Three cases of IEPS were diagnosed prenatally by ultrasound in our center.The data of these 3 cases and 43 cases in prior studies from 1986 to 2016 were retrospectively reviewed.The prenatal sonographic features,treatment,and outcomes were collected.Results The gestational age of diagnosis IEPS was (24.5±5.9)weeks,the male-to-female ratio was 3-4∶1,78.3% (36/46) of IEPS masses located on the left side,47.8% (22/46) of lesions were homogenous and hyperechoic,8.7% (4/46) of cases associated anomalies,78.3% (36/46) of patients were preformed resection and postoperative recovery and subsequent course were uncomplicated.Feeding arteries were detected in 15.2% (7/46) cases and confirmed by surgery or autopsy.Conclusions Prenatal ultrasound has the diagnostic accuracy of IEPS.Feeding arteries noted on color Doppler are strongly indicators of IEPS.Postsurgical outcomes are favorable.

Objective Gas chromatography-mass spectrometer (GC-MS) technology combined with Heuristic evolving latent projections (HELP) method were used to qualitatively analyze the volatile oil of fructus lycii (Lycium barbarumL.)Methods With the best temperature-programmed chromatographic condition, overlapping peaks among the total ion chromatogram were separated. Then an automatic mass spectral deconvolution and identification system (AMDIS) was used to identify volatile components comprehensively. Finally, the pure chromatographic peaks were matched with NIST 05a mass spectra library for giving the precise qualitative result. Results Totally, 29 compounds in fructus lycii (Lycium barbarum L.) were identified accurately.Conclusions Compared with the traditional qualitative analysis method, the new established method which GC-MS technology combined with HELP could get tremendous advantages. It not merely enhanced the accuracy of identification but also solved the bottleneck of handling the inseverable compounds.

Objective To observe the clinical efficacy of hydro-medicinal cupping using Chinese medicinal granules in treating discogenic low back pain. Methods Sixty patients with discogenic low back pain were randomized into a treatment group and a control group, 30 in each group. The two groups were both intervened by hydro-medicinal cupping at Yaoyangguan (GV 3) and two sides of the point, while the Chinese medicinal granules were used in the treatment group and the conventional form of medicine was used in the control group. The pain score was observed before treatment and after 4 treatment courses, and the clinical efficacies were also compared. Results The pain scores were significantly changed after intervention in both groups (P<0.01). After intervention, there was no significant difference in comparing pain score between the two groups (P>0.05). The total effective rate was 93.3%in the treatment group versus 96.7% in the control group, and the difference was statistically insignificant (P>0.05). Conclusions Hydro-medicinal cupping is an effective approach in treating discogenic low back pain, and there is no marked difference in efficacy between the Chinese medicinal granules and conventional form of medicine.