OBJECTIVE:To study endothelial NO-dependent vasodilation effect of the extracts of Herba Siegesbeckiae. METHODS:SD rat thoracic aorta rings were used to observe contraction of blood vessel, and the effects of the extracts of Herba Siegesbeckiae on the contraction induced by phenylphrine (PE). RESULTS:The extract of Herba Siegesbeckiae could inhibit the contraction of blood vessel induced by PE. After pretreatment of blood vessel by L-NNA, vasodilation effect of the extracts of Herba Siegesbeckiae was significantly attenuated at low concentration condition(P

Objective To perform statistical analysis of low frequency electromagnetic comprehensive therapy apparatus for adverse events,and the characteristics of the adverse events,and investigate the risk factors,thus to provide effective information for the safe of the medical device.Methods Using database software from national MDR monitoring center,preliminary study on safety of low frequency electromagnetic comprehensive therapy apparatus was performed.Results The manifestations of adverse events was 50.0%,mainly included skin allergy and scald,burn, stabbing pain,25.0% included treatment without feeling,parts damaged,other manifestations accounted for 25.0%. Conclusion Monitoring should be strengthened in order to reduce or avoid the vaginal dilator adverse events.

Objective To investigate the characteristics of rifampin(RFP) and isoniazid(INH) resistance genes of Mycobacteri-um tuberculosis in Ganzhou region .Methods Gene chip technology was employed to detect common resistance genes in 130 sputum samples with smear positive acid-fast staining and Ct values less than 30 .Results Among 130 samples ,34 samples(26 .2% ) were found resistance ,including 30 samples(23 .1% ) resistant to RFP ,26 samples(20 .0% ) resistant to INH ,and 21 samples(16 .2% ) resistant to both .The mainly mutation of RFP resistant gene was rpoB gene 531(C→ T ) ,accounting for 42 .4% ,while the mainly mutation of INH resistant gene was katG gene 315(AGC→ACC) ,accounting for 65 .4% .Conclusion The mutation charateristics of RFP and INH resistance-associated genes of Mycobacterium tuberculosis in Ganzhou region is consistent with domestic and foreign research .

Objective:To study the anti-tumor effects by vaccination of mice with murine melanoma B16 cells expressing calreticulin (CRT) and Human papillomavirus 16 E2 fusion protein.Methods:Mice were inoculated with B16 cells expressing CRT,E2,or CRT-E2 by intraperitoneal injection. The inoculation was repeated after one week. The activity of CTLs and NK cells,the proliferation of T cells and the amount of IFN-? secreted by spleen lymphocytes were analysed.Results:The activity of CTLs and NK cells,and the amount of IFN-? secreted by spleen lmyphocytes from mice vaccinated with CRT-E2 fusion protein were significantly higher than those of the other groups(P

Objective To study the blood pressure and cardiovascular conditions in kidney transplant recipients in order to prevent the cardiovascular complications in such patients. Methods The blood pressure and ECG were monitored day and night by noninvasive means for 86 patients after transplantation.The results were analysed and compared with the pretransplantation studies. Results Hypertension occurred in 55 out of 86 patients.There was a significant difference of average blood pressure and the occurrence of abnormal ST segment or T wave between day and night whereas no significant change was observed in blood CsA concentration,blood creatinine,serum cholesterol,blood sugar,triglyceride and K+,N+,Cl-,TCO 2,Ca 2+ ,etc. Conclusions Cardiovascular complications in kidney transplant recipients is related to the high blood pressure during night,the normal day-night blood pressure rhythm being violated.It is not related to the immunosuppresives used.

Objective To evaluate the use of isoptin in kidney transplants recipients. Methods The graft acute rejection rate,the cyclosporin A daily dose and its trough level and toxicity,the blood pressure and cardiovascular complications,the serum creatinine and blood sugar were studied in cadaveric kidney transplant recipients both with and without the additional use of isoptin. Results With the additional use of isoptin in 44 cadaveric kidney transplant recipients,the acute rejection rate has not increased.After using isoptin for 2 weeks,CsA blood level has been increased 25.5% whereas the CsA dose been cut down 32.6 %.So,drug expense dropped about 30.0% and CsA toxicity (hand tremble,insomnia) was slightly decreased. Conclusions Isoptin does not inecease the graft acute rejection rate yet makes the CsA dose and its toxicity less.

OBJECTIVETo investigate the risk factors of vaginal dilators by 567 adverse event reports, and to provide a reference for the reasonable use.

METHODSWith retrospective case study, analyzed 567 reports induced by vaginal dilators by National Adverse Drug Reaction Monitoring Center in 2012.

RESULTSExpected treatment of disease might be relevant with severity of adverse events, while age was not the related factor; the influencing factor of consequences of grading was the classification of the cause of adverse events.

CONCLUSIONMonitoring should be strengthen in order to reduce or avoid the vaginal dilator adverse events.

Adverse Drug Reaction Reporting Systems ; Dilatation ; adverse effects ; Drug-Related Side Effects and Adverse Reactions ; epidemiology ; Female ; Humans ; Retrospective Studies ; Risk Factors ; Vagina ; pathology

Objective To evaluate the efficacy of dezocine for prevention of postoperative cognitive dysfunction (POCD) in elderly patients undergoing total knee arthroplasty under remifentanil-based anesthesia.Methods Sixty-eight patients of both sexes, aged 65-85 yr, weighing 48-78 kg, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ , undergoing elective unilateral total knee arthroplasty under general anesthesia, were randomly divided into 2 groups (n =34 each) using a random number table: control group (group C) and dezocine group (group D).After induction of anesthesia, the patients were tracheally intubated and mechanically ventilated.Anesthesia was maintained with iv infusion of propofol 0.05-0.06 mg · kg-1 · min-1 and remifentanil 0.05-0.10 μg · kg-1 · min-1, and intermittent iv boluses of vecuronium 0.04 mg/kg.Dezocine 0.1 mg/kg was injected intravenously at 30 min before skin incision in group D.At 1 day before operation (T0) , and 1, 3, 5, 7 days after operation (T1 , T2, T3 , T4) , the blood samples from the central vein were collected for determination of serum cortisol (Cor), neuron specific enolase (NSE) and S-100β protein concentrations.Before operation, and 5 and 7 days after operation, the patients' cognitive function was assessed using Mini-Mental State Examination, and the occurrence of POCD was recorded.Results Compared with the baseline value at T0, the serum Cor, NSE and S-100β protein concentrations were significantly increased at T1-3, and MMSE scores were decreased at T3,4 in the two groups.Compared with group C, the serum Cot, NSE and S-100β protein concentrations were significantly decreased at T1-4, and Mini-Mental State Examination scores were increased at 5 and 7 days after operation, and the incidence of POCD was decreased in group D.Conclusion Dezocine 0.1 mg/kg intravenously injected at 30 min before skin incision can prevent the occurrence of POCD in elderly patients undergoing total knee arthroplasty under remifentanil-based anesthesia.

Objective To investigate the effect of the new polyamine analogue tetrabutyl propanediamine (TBP) on cell proliferation and the underlying mechanism. Methods MTT assay was performed to determine cell proliferation. Flow cytometry was performed to detect cell cycle transition. DNA fragmentation and mitochondrial membrane potential determinations were performed to detect cell apoptosis. The activity of key enzymes in polyamine catabolism was detected by chemiluminescence assay. Results TBP could significantly inhibit the proliferation of HL-60 cells by blockingcell cycle transition and by inducing apoptosis. The TBP-induced apoptosis of HL-60 cells was in a dose-dependent manner. The enzyme activities of SMO and APAO were also significantly increased in HL-60 cells after treatment with 100 μM TBP for 24 hours. Conclusions TBP, as a new putrescine analogue, could inhibit proliferation of HL-60 cells by increasing the enzyme activity of SMO and APAO and inducing apoptosis.

Objective To investigate the effect of S-1 on serum thymidine kinase-1 ( TK1 ) , vascular endothelial growth factor ( VEGF ) and insulin-like growth factor-1 (IGF-1) and tumor biomarkers, estrgen levels and quality of life in patients with advanced breast cancer.Methods 69 cases with advanced breast cancer from January 2014 to May 2015 in the hospital were selected and randomly divided into two groups.34 cases in control group were treated by conventional therapy, and 34 cases in experimental group were treated with S-1.Serum TK1, VEGF and IGF-1, tumor biomarkers, estrogen level and quality of life score were compared pre-and post-treatment.ResuIts Compared with control group, the levels of VEGF, IGF-1 and TK1 were lower (P<0.05), serum CA125, CEA and CA15-3 concentrations were lower (P<0.05),the LH, E2 and E1 levels were lower (P<0.05) and the survival quality score was higher in experimental group ( P<0.05 ) .ConcIusion S-1 has better clinical curative effect in treatment of patients with advanced breast cancer, and effectively reduce serum TK1, VEGF, IGF-1, tumor biomarker levels, improve the quality of survival, which has important significance.