With the illustration of the L-Arg-NO-cGMP pathway in penile erection,the researches on nitric oxide synthases(NOSs),which produce nitric oxide(NO)as the main messenger to control the process,has stayed in the spotlight.nNOS locates in neuroal tissues and eNOS is binding on the membrane of cavernous sinus endothelium with caveolin.The nNOS & eNOS are expressed constitutively.The activation of nNOS & eNOS is more dependent on Ca2+ concentration than iNOS,which is seldom expressed except in aged or pathological conditions.It is now clear that nNOS may initiate cavernosal tissue relaxation under the regulation of PDZ zone when transcripted,while phosphorylated eNOS may facilitate attainment and maintenance of full erection.iNOS expression stimulated by the cytokines may be beneficial(cytoprotective or anti-fibrotic)or deleterious(cytotoxic)according to the tissue environment.Gene therapies based on NOSs have attained a great deal of achievements in animal experiments and will promise a wonderful future.

Objective　To study the roles of P-selectin and nitric oxide (NO) on the etiology of pregnancy induced hypertension (PIH). Methods　The levels of plasma P-selectin and nitrite and nitrate (NO2-/NO3-) were determined in 36 patients with PIH. The data were compared with those of normal pregnant group (n=18) and healthy non-pregnant group (n=19). The levels of plasma P-selectin were measured by means of enzyme-linked immunosorbent assay (ELISA) and levels of plasma NO2-/NO3- were measured by Griess reaction after the nitrate was reduced by a Cadmium-reduction method. Results　 The results showed: （1）The level of P-selectin in the normal pregnant group (48.92±15.16) μg/L was higher than that in non-pregnant group ［(30.16±8.42) μg/L, P<0.001］. There was no significantly statistical difference in the levels of P-selectin between the normal pregnant group and the mild PIH group (P>0.05). The levels of P-selectin in the moderate and severe PIH group (70.11±17.65) μg/L were higher than those in the mild PIH group ［(47.33±17.95) μg/L, P<0.01］ and the normal pregnant group (P<0.001). （2）The concentration of NO2-/NO3- in normal pregnant group was higher than that in non-pregnant group (P<0.01). The concentration of NO2-/NO3- in PIH group were lower than was in normal pregnant group (P<0.001). The NO2-/NO3- in mild group was lower than that in normal pregnant group (P<0.001). The Plasma concentration of NO2-/NO3- in moderate and severe PIH group was lower than that of mild group (P<0.05). （3） There was a negative correlation between the plasma concentration of P-selectin and NO2-/NO3- in the PIH group. Conclusion　The changes of plasma levels of P-selectin and NO are related to the occurrence and development of PIH.

Objective　To study the association of plasma von willebrand factor (vWF) and endothelin-1(ET-1) levels in patients with pregnancy induced hypertension (PIH). Methods　Plasma vWF and ET-1 were studied by ELISA method and immunoradiological method respectively in 36 patients with PIH, 18 normal pregnant women and 19 normal non-pregnant women. Results　Circulation levels of vWF and ET-1 were increased with increasing severity of the PIH. In mild PIH patients, the levels of plasma vWF ［(135.9±30.9)%, （P>0.05)］ weren′t significantly higher than those in healthy pregnant women ［(131.6±39.2)%,（P>0.05)］，but the levels of ET-1 ［(63.7±4.8) pg/L］ were significantly higher than those in healthy pregnant women ［(47.1±4.7) pg/L,（P<0.01)］. There were significant differences between groups of moderate-severe PIH ［vWF (174.4±35.4)%, ET-1 (92.6±19.1) pg/L］. There was a positive correlation between the plasma vWF level and ET-1 level. Conclusion　The results suggested that plasma vWF and ET-1 concentration could be used as indicators for the severity of PIH.

Objectives: To evaluate the effect of thickness and opaque resin on the light transmittance of Artglass. Methods: Samples of Artglass prepared in the thickness(mm) of 0.50,0.75,1.00,1.25,1.50,1.75 and 2.00 with or without opaque resin respectively.The light transmittance of the specimens was measured. The color values of the specimens on the CIE L*a*b* color system were determined by a digital chroma meter. Results: The light transmittance of Artglass decreased logarithmically with the increase of the thickness (R2=0.9834 in the group of samples without opaque and R2=0.9390 in those with opaque). The light transmittance of Artglass with opaque resin was lower than that without opaque resin. Conclusion: Thickness and opaque resin have significant effect on the light transmittance of Artglass.

Objective To explore the curative effect of the pressure sore treated by iodophors associating with oxygen and peptide. Method 36 cases with pressure sore in stage Ⅲ and stage Ⅵ were randomly divided into the observation group and the control group. After ebriding was carried out in both groups, the control group was trea-ted with the rout change dressing and the other group was treated by iodophors associating with oxygen and peptide es-pecially. Result The cicatrization time of the observation group was remarkablely shorter than the control group. Conclusion Treating the pressure sore by iodopbors associating with oxygen and peptide has the advantage of less passion, shorter course of treatment, better curative effect, economy and mote convenience which is worth for the pro-moting of clinical usage.

Objective: To sum up characteristics of hotline counseling about mental health problems in Shanghai. Method: All records of hotline counseling from 1990 to 2000 were input into computer. Retrospective analysis was done. Results: In the past 10 years, the main issues in hotline counseling were associated with love affairs (18.1%), emotional troubles (15.8%), psychosis (11.3%), and interpersonal relationship (8.1%), which were also related to the help-seekers background, such as gender, age, education levels, occupation and marriage status.

Objective To determine whether trefoil factor 1(TFF1) could be associated with healing of gastric mucosa and differentiation of gastric carcinoma. Methods TFF1 in normal and various pathologic gastric mucosa was assessed by immunohistochemical method and analyzed with Motic Images Advanced 3.0 software. Results Compared with normal mucosa, increased TFF1 was detected in gastritis, gastric ulcer and duodenal ulcer. TFF1 was in increased expression in multiple and compound ulcer than simple ulcer. There was no significant difference among gastric ulcer, duodenal ulcer, gastritis and simple ulcer. Increased TFF1 was detected in peripheral mucosa of the gastric adenocarcinoma as compared with normal mucosa. The expression of TFF1 in gastric adenocarcinoma was related to the differentiation of adenocarcinoma, that is, the more poorly differentiated, the lower expression of TFF1. Conclusion TFF1 may play a significant role in gastric mucosa protection and epithelial restitution. TFF1 may also contribute to the differentiation of gastric adenocarcinoma.

Objective To study the method of biological dye injection for detecting sentinel lymph node(SLN) in breast cancer, and explore the accuracy of evaluating axillary lymph node status by SLN biopsy(SLNB). Methods Intro-dermal methylthionium injection during operation was applied to identify SLN in 71 breast cancer patients, and the detection results were compared with the metastasis status of dissected axillary lymph nodes. Results SLN was identified in 70 of 71 patients(97%) using methylthionium injection. Total 188 SLNs were detected in all patients, each patient having 1～4 SLNs(average 2 7). The false negative rate of methylthionium injection for detecting SLN was 2%(1/42). The sensitivity, specificity and accurate rate of SLN to predict axillary lymph node metastasis status were 96%, 100% and 97% respectively. Conclusion Intro-dermal methylthionium injection can identify SLN and accurately predict the axillary lymph node status . This method is cheap and can be widely used.

【Objective】To investigate the relationship between the expression of lymphocyte function-associated antigen-1 (LFA-1) on peripheral lymphocytes and acute rejection after orthotopic liver transplantation (OLTx) in rat.【Methods】Adult male rats were divided into 2 groups.In the non-rejection group,40 SD rats were used as both donors and recipients.In the rejection group,20 Wistar rats were used as donors and 20 SD rats as recipients.Blood samples were collected through the tail vein 1 day before transplantation and on days 1、3、5、7 after OLTx.The expression of LFA-1 (CD11a) on peripheral lymphocytes was analyzed by using indirect immunofluorescent marker-flow cytometry.【Results】The expression level of LFA-1 on peripheral lymphocytes in recipient rats after orthotopic liver transplantation was markedly lower than that before operation (P<0.01);The expression level of LFA-1 on peripheral lymphocytes in rats with acute liver rejection was significantly higher than that in the non-rejection group (P<0.01).【Conclusion】Monitoring the expression level of LFA-1 on peripheral lymphocytes may be helpful to the diagnosis of acute graft rejection.

Objective To investigate the diagnostic value of MSCT with multi-planar reconstruction(MPR)and maximum intensity projection(MIP)in diagnosis of spinal burst fracture.Methods 45 patients(53 vertebras)with vertebral burst fracture were examined by MSCT and processed with MPR and MIP.The imaging features were analyzed comparatively.Results The axial images clearly demonstrated the vertebral body vertically or transversely burst crack in 49 vertebras(92.5%),bony fragment inserted into the spinal canal and stenosis of spinal canal in 34 vertebras(64.2%).The sagittal images showed kyphosis in 28 vertebras(62.2%).The sagittal and coronal images showed decreased height of the vertebral body in 37 vertebras(69.8%)and depressed fracture of vertebral end plate in 19 vertebras(35.8%).Total 44 fractures were located at spinal appendix,39 were showed by axial images,35 by sagittal images and 33 by coronal images.MIP displayed the space changes of bone structures in all cases and rotary dislocation fracture in 6 cases(11.3%).Conclusion MPR and MIP are of significant values in diagnosis and clinical treatment of spinal burst fracture.