Objective To investigate the characteristics of peripheral pulmonary hamartoma with multi-slice CT, in order to improve the accuracy of diagnosis of the disease. Methods The multi-slice CT manifestations in 16 peripheral pulmonary hamartoma patients who were confirmed by pathology were retrospectively analyzed. Results Most of the patients were in middle age, whose ages were 22 - 67 years old, and the average age was 43.2 years old. All the patients had solitary pulmonary nodules, with clear boundary, with 7 cases in right lung and 9 cases in left lung. Pulmonary nodules were round in 6 cases, similarity round in 7 cases and irregular shape in 3 cases, among which 1 case had shallow lobulation and 2 cases had deep lobulation. Two cases had pleural up syndrome, and 1 case had vascular convergence sign. The diameter of the lesions was 1.5 - 5.2 cm, and the average was 2.48 cm, with 1 case >5 cm. One case showed a typical popcorn calcification. There were stippled calcification in 2 cases and annular calcification in 1 case. Intralesional fat only showed in 3 cases, and both fat and calcification in 2 cases. Seven cases showed homogeneous soft tissue density, neither fat nor calcification. Enhanced scan: 5 cases had no abnormal enhancement, 11 had mild enhancement of soft tissue. Conclusions Fat and calcification are the typical signs of peripheral pulmonary hamartoma. Clear boundary and no enhancement or slight enhancement is also the important diagnostic basis, and lobulation and pleural up syndrome do not exclude the possible pulmonary hamartoma.

Objective To analyze the plain and enhanced features of multiple spiral CT in primary splenic tumors,and to evaluate the diagnostic value.Methods The spiral CT findings of 29 cases of splenic primary tumors proved by operation or biopsy were retrospectively analyzed.Results 23 cases of benign tumors (9 cases of splenic cyst,7 cases of hemangioma,3 cases of lymphangioma,2 cases of myofibroblastic tumor,hamartoma in 2 cases),clinical manifestations of mild abdominal discomfort or no symptoms;CT scan for spleen in single or multiple low density or mixed density mass.Hemangioma,wrong structure can show the tumor calcification spots,hamartoma with calcification or fat density.CT enhancement,no enhancement of splenic cyst,hemangioma and delayed enhancement,center filling;lymphangioma without slight strengthening or interval,myofibroblastic tumor or hamartoma to strengthen the characteristic of diversity.6 cases of malignant tumors (3 cases of lymphangioma,2 cases of lymphoma,1 case of malignant fibrous histiocytoma of angiosarcoma),malignant tumors had abdominal pain,weight loss and other symptoms,CT scan,spleen enlargement,mass was larger,the boundary was not clear,less calcification,can strengthen the CT,tumor metastasis;lymph enhanced slightly,angiosarcoma delayed enhancement,uneven enhancement of malignant fibrous histiocytoma.Conclusion Multi slice spiral CT scanning combined with enhancement has a high diagnostic value for primary splenic tumors.

Objective To evaluate the hot and cold characteristics of 10 antibiotics.Methods MTT assay was used to investigate the effects of 10 antibiotics on the growth and proliferation of cultured SMMC7721 cells and MFC-7 cells in vitro .Morphological changes were observed under the inverted microscope.Results The 10 antibiotics,namely,ampicillin,cefixime,cefpodoxime proxetil,cefaclor,cefalexin,azithromycin,clarithromycin, roxithromycin,doxycycline and oxytetracycline showed cold or cool characteristics.Morphological observation showed that cells treated with these drugs presented decreased cell density and turned round.Conclusion The results of this study demonstrated that the cytological method can be used to evaluate the hot and cold characteristics of western drugs.This simple and reliable method will promote research on Chinese medicalization of western drugs.

AIM: To investigate the adhesion between lymphocytes and extracellular matrix (ECM) in delayed-type hypersensitivity (DTH) in mice as well as the regulatory effect of several cytokines on it. METHODS: DTH was induced by the application of picryl chloride (PCl) to mice that had been sensitized twice by PCl, followed by isolating the spleen lymphocytes at different times. The adhesion of spleen lymphocytes to fibronectin or typeⅠcollagen were examined. In some cases, spleen lymphocytes obtained at 18 h were incubated with various concentrations of IL-2, IFN-?, and TNF-? for 4 h at 37℃ before the adhesion assay. T cells purified from the spleen lymphocytes were also used for the assay in the presence of TNF-?. RESULTS: The adhesion of spleen lymphocytes to ECM began to increase at 6 h after PCl challenge, reached a peak at 18 h, then decreased and returned to normal level at 36 h. IL-2 significantly increased the adhesion of lymphocytes to ECM at a dose of 1?10 5 U/L and TNF-? enhanced the adhesion in a concentration-dependent manner. The combined use of TNF-? and IFN-? showed a significantly enhanced adhesion. TNF-? was more effective to accelerate the adhesion of spleen T Cells than that of total spleen lymphocytes. CONCLUSION: In the process of DTH, the adhesion of lymphocytes to ECM was regulated by several cytokines and associated with the development of inflammation.

Objective To establish a new method to identify and analyze Qingdai Powder and its composition.Methods X-ray Diffraction with Fourier pattern was used.Results The standard X-ray diffraction with Fourier pattern and characteristic diffraction peaks of Qingdai Powder and its composition drugs were obtained.Conclusion The powder X-ray diffraction method can be used as a simple and convenient method for the identification of Qingdai Powder and its composition drugs.

Objective To compare the effects of one year treatment of estrogen and tamoxifen, either alone or combined with fluoride on bone metabolism in ovariectomized rats Methods One hundred and forty two virgin female Sprague Dawley rats were ovariectomized (OVX) or sham operated at 6 months of age, and randomly divided into 7 groups (19-21 rats each): (1) sham operated controls; (2) OVX vehicle controls; (3)estrogen group; (4) fluoride group; (5) tamoxifen group; (6) fluoride plus estrogen; (7) fluoride plus tamoxifen The treatments lasted for one year Bone mineral density (BMD) measurement, bone histomorphometry analysis (lumbar vertebrae) and biomechanical test (3 point bending test on right femur) were performed before and after treatment The histological change of uterus were also determined at the same time Results (1) Twelve months of ovariectomy, the OVX group had significantly lower total body (279 mg/cm 2), lumbar vertebra (232 mg/cm 2) than all other groups (total body 286-298 mg/cm 2, lumber 251-266 mg/cm 2, P

Objective To evaluate the effects of sevoflurane preconditioning on caveolin-3 expression during myocardial ischemia-reperfusion (I/R) in rats.Methods Healthy male Wistar rats,aged 6-8 weeks,weighing 250-300 g,were anesthetized with intraperitoneal pentobarbital 30 mg/kg and heparin 1 000 IU/kg.Their hearts were excised and perfused with K-H solution in a Langendorff apparatus.Thirty isolated rat hearts were randomly assigned into 3 groups (n =10 each) using a random number table:control group (group C),I/R group and sevoflurane preconditioning group (group SP).After 30 min of equilibration,group C was continuously perfused with K-H solution for 90 min,group I/R underwent 30 min of ischemia followed by 60 min of reperfusion,and group SP was perfused with K-H solution saturated with 2％ sevoflurane for 10 min followed by 5 min washout with K-H solution,then underwent 30 min of ischemia followed by 60 min of reperfusion.HR,left ventricular enddiastolic pressure (LVEDP),+ dp/dtmax and-dp/dtmax were recorded at the end of equilibration,immediately before ischemia and at 30 and 60 min of reperfusion.Myocardial specimens were obtained from the cardiac apex for microscopic examination.Myocardial specimens were obtained from the left ventricle for determination of caveolin3 expression.Results Compared with group C,+ dp/dtmax and-dp/dtmax were significantly decreased immediately before ischemia,and HR,LVDEP,+ dp/dtmax and-dp/dtmax were decreased at 30 and 60 min of reperfusion in groups I/R and SP,and caveolin-3 expression was down-regulated in group I/R and up-regulated in group SP (P ＜ 0.05).Compared with group I/R,HR,LVDEP,+ dp/dtmax and-dp/dtmax were significantly decreased immediately before ischemia and increased at 30 and 60 min of reperfusion,and caveolin-3 expression was up-regulated in group SP (P ＜ 0.05).The pathological changes were significantly attenuated in group SP as compared with group I/R.Conclusion The mechanism by which sevoflurane preconditioning attenuates myocardial I/R injury in rats may be related to up-regulation of myocardial caveolin-3 expression.

Objective:To study the influence of the DCs from mononuclear cells in axillary draining lymph nodes of patients with breast cancer to the cytotoxicity of the antigenic specific CTLs stimulated by freeze-thrawing antigen from breast cancer cells in vitro.Methods:The mononuclear cells were isolated from axillary draining lymph nodes,and were cultured with cytokines to induce DCs and tumor draining lymph node cells(TDLNCs) respectively.DCs stimulated by the auto-breast cancer freeze-thrawing antigen(DCs-Ag).TDLNCs were co-cultured with DCs-Ag to derive tumor antigenic specific CTLs(DC-Ag-TDLNC).Then the cytotoxicty of specific CTLs to auto-breast cancer cells and MCF-7 cells was detected.Results:The cytotoxicity of DC-Ag-TDLNC,DC-TDLNC and TDLNC to the auto-breast cancer cells was 67.64%、31.25% and 26.36% respectively,P0.05.Conclusion:Typical DCs can be induced from the mononuclear cells from axillary draining lymph nodes after stimulated with cytokines(rhGM-CSF,rhIL-4 and TNF-?).The DCs possess stronger antigen presentation and can obviously increase the killing activity of tumor antigen specific CTLs to auto-breast cancer cells.

Objective To explore the clinical experience of liver retransplantation in management of biliary complications after liver transplantation.Methods The clinical data of patients receiving liver transplantion in our department between April 2002 and Aug. 2004 were retrospectively analyzed, including 5 patients being subjected to liver retransplantation because of serious biliary complication.Results Three patients were cured with the survival time being 5 months, 6 months and 8 months respectively. Two patients died at 8th and 43rd day: one died of liver non-function combining with lung infection and heart failure, and another died of renal failure. Complications included liver absess, biliary infection, wound infection, hydrops abdominis and lung infection.Conclusions Liver retransplantation is an effective treatment for serious biliary complications after liver transplantation. Surgical procedure, indication, operative opportunity, perioperative monitoring and so on contribute to the increase of the survival rate of the patients receiving liver retransplantation.

Objective To analyze the MRI features of intracranial ectopic germinomas compared with pathologic findings.Methods MRI manifestations of intracranial ectopic germinomas in 24 cases verified by operation and pathology were retrospectively analyzed.Results Tumors were located at suprasellar region in 5 cases,basal nucleus region in 6 cases,round lateral ventriculus in 2 cases,pellucid septum and lateral ventriculus in 1 case,3 cases suprasellar and pineal region in 3 cases,basal nucleus and pineal region in 3 cases,pineal region and round lateral ventriculus in 2 cases,lateral ventriculus,and third ventricles as well as pineal region in 2 cases.Lesions were showed marked enhancement in 22 cases and 10 cases had cystic degeneration on MRI.Pathologically,18 of 24 cases were seen clear border,cystic degeneration 11 cases,hemorrhage 2 cases and calcification 2 cases.Conclusion Intracranial ectopic germinomas are common in male children and are of MR characteristics,its MRI findings are correlated with pathology.