Objective To analyze the causes of misdiagnesis and mistreatment of traumatic abdominal hernia. Methods In this study,11 cases of traumatic abdominal hernia admitted during 1971 to 2004 were retrospectively analyzed.Hernia located subcutaneously in 8 cases including incarcerated hernia in 2 csses.Hernia located intermuscularly in 2 cases with one case being incarcerated.and the other strangulated.One located retroperitoneally.Among the 11 cases,3 were simple hernia,8 were complicated with injuries of other organs. Results Eight cases were misdiagnosed,5 were operated on with wrong incision,and in one case the herniated intestinal loop,mistaken as hematoma,was incised.All these 5 cases underwent a second surgery due to failure in repairing the hernia. Condusion For traumatic abdominal wall hernia,correct diagnosis,the correct timing of operation and an appropriate incision chosen insure good therapeutic results.

The expression of proliferating cell nuclear antigen (PCNA) was studied in 50 cases of squamous cell carcinoma of the skin using immunohistochemistry and PCNA cyclin monoclonal antibody. PCNA staining was found in the nuclei of tumour cells, two types of staining were seen, a granular pattern and a more diffuse nuclear staining. The expression of PCNA showed a significant positive correlation with degrees of differentiation of the carcinoma cells and lymph node metastasis (P

The expressions of p53 protein, PCNA and macrophage count in 50 cases of skin squamous cell carcinoma were studied by LSAB immunostaining of monoclonal antibodies (PC-10, CD68 and Do-7). The relationship between the proliferation of cancer cell and the count of stroma macrophage to the degree of differentiation was studied and observed in situ. The results showed that the positive rates of expression of both p53 protein and PCNA in the squamous cell carcinoma were 56% and 100%, respectively. The distribution and strength of the expression of p53 protein and PCNA were positively correlated to the degree of cell differentiation. Macrophoge regularly located in the margin of cancer nest. The macrophage count was reversely correlated to the degree of differentiation. It is concluded that the degree of differentiation is parallel to the expression of p53 protein and PCNA, whereas, the macrophages in the stroma to some extent inhibits the cancer cell proliferation.

The skin organ model can be cultrured in ways of organ culture and organotypic raft culture It can be used in skin transplantation,pharmacy or investigate the mechanism of pigmentation. Though there are many ways in culturing skin organ model,how to further improve technology to produce more similar to human's skin organ,and put it in treating dermatosis still needs further exploring. The model of skin organ provides a good platform for new drug development,toxicology experiment and physiological function research. Some tissues engineering skin products have been approved by Food and Drug Administration in America for clinical use. It is believed that following the development of tissue engineering and relative subjects,skin organ culture model in vitro will become one of the effective means on dermatosis study.

The paper makes detailed contrastive analysis of the grade protection system and classified protection system in the process of system construction from the applicable scope , construction procedure , system grading , schematic design , system implementation , sys-tem evaluation and inspection , information system halt, methods of bidding and purchasing , etc.By comparison, these two systems are different in the implementation object , competent department , construction procedure , bidding and purchasing , etc.

Objective To investigate correlation study of serum hs-CRP,UA level and the correlation of dis-ease in elderly acute myocardial infarction patients , and provide a reference basis for clinical judgment for patients with illness development and prognosis prediction .Methods 40 aged patients with acute myocardial infarction pa-tients were selected as the research object ,and protionally random according to time admission the same period ran-domly selected were 40 cases of elderly patients with unstable angina pectoris ,40 cases of elderly patients with stable angina,40 cases of clinic medical normal healthy old people for controlled object .The levels of serum hs-CRP,UA and correlation was analyzed.Results The serum hsCRP,UA in the myocardial infarction group were (34.67 ±12.18)mg/L, (412.46 ±68.93)μmol/L,which in the unstable angina pectoris group were (17.21 ±8.39) mg/L,(274.19 ± 46.62)μmol/L,which in stable angina pectoris group were (5.25 ±1.40) mg/L,(297.41 ±37.43)μmol/L,which in the health group(4.96 ±1.24)mg/L,(279.64 ±34.78)μmol/L,the differences among the myocardial infarction group,unstable angina pectoris,stable angina pectoris group had statistical significance (P<0.05), the differences between myocardial infarction group and health group had statistical significance (P<0.05);Myocardial infarction patients prognosis group hs-CRP,UA group was obviously higher than that of the prognosis was good ,compared with statistical significance difference ( P <0.05 );In myocardial infarction group , unstable angina pectoris group , the hsCRP was associated with positive correlation exists between UA (P<0.05),stable angina pectoris group no correla-tion(P>0.05);Different prognosis in patients with myocardial infarction hsCRP is associated with UA has positive correlation between ( P<0.05) .Conclusion Elderly acute myocardial infarction patients serum hs-CRP,UA obviously increased ,and illness development correlation ,a high level of hs-CRP,UA patients often indicates poor prognosis .

Objective To evaluate the effect of montelukast combined with seretide in treatment of patients with asthma and its influence on balance between Th17 and Treg cells, and expression of IL-4 and IFN-γ.Methods 110 patients with asthma were randomly divided into observation group and control group, observation group of 55 cases, using montelukast combined with seretide, control group of 55 cases, using seretide,compared two groups of patients with pulmonary function changes, peripheral inflammatory factor changes and influence on Th17 and Treg cells balance. Results Before treatment, observation group and control group,FEV1 and FEV1 /FVC value was no significant difference.FEV1 and FEV1 /FVC after treatment were significantly improved compared with before treatment in two groups, differences were statistically significance (P<0.05).Between two groups, FEV1 values were significantly difference (t=8.1156, P=0.0001), FEV1 /FVC also had significant difference (t=4.8933, P=0.0001) .Before treatment, observation group and control group, IL-4 and IFN-γdid not have a statistically significant.After treatment, IL-4 and IFN-γdecreased significantly, with statistical difference compared with before treatment (P<0.05).Between two groups, IL-4 had significant difference (t=6.0273, P=0.0001), IFN-γalso had statistically significant (t=6.6042, P=0.0001).Before treatment, there was no significant difference between Th17 and Treg percentage, after 12 weeks of treatment and 24 weeks, Th17 in observation group were(0.53 ±0.02),(0.32 ±0.01), the control group were (0.78 ±0.03),(0.62 ±0.02), which had differences (P<0.05).After 12 weeks and 24 weeks,observation group Treg respectively(2.21 ± 0.22),(2.47 ±0.10),in control group were(1.50 ±0.11),(1.84 ±0.14), Treg percentage at different time after treatment, the differences had statistically significant (P<0.05).Conclusion Montelukast combined with Seretide in treating with asthma, pulmonary function of patients is obviously improved, and it can improve Th17, Treg cell.

ObjectivesTo evaluate the therapeutic result of various management for severe radiation injury of the bowels.MethodsThe clinical presentation, diagnostic approach, managements and therapeutic effects were analysed retrospectively in 28 severe radiation therapy caused enteric injury patients. In all intestinal stricture cases, partial ileal resection was performed in 14 patients, right hemicolectomy in 1 patient, rectosigmoid resection in 3 patients. Instestinal fistulae was treated by diseased segment resection and reanastomosis in 7 patients, sigmoidostomy was undertaken in 2 patients.ResultsThere was no inhospital mortality. One case suffered from low colonic fistula,4 died of recurrence of the primary tumor.Postoperative bowel function and nutrition status significantly improved in all patients.ConclusionSurgical management combined with nutrition support is safe and effective for the treatment of severe radiation caused enteric injury.

Objective To explore an effective method of repairing the old rupture of patellar tendon. Methods We designed a new 井-shaped apparatus for traction and fixing which was to be used, under the dynamic conditions, to reduce the patella, loosen the quadriceps m. of thigh, repair the tendon of knee cap and facilitate postoperative exercises with the fixing apparatus on. Results We used the apparatus to repair the tendon of knee cap in 6 cases. The follow up of 8 to 56 months (averaging 26 months) showed that their functions of the knee joint were all satisfactory. Conclusions The 井-shaped external fixation device can be used under the dynamic conditions to repair the old rupture of patellar tendon with the advantages of little contracture of the knee cap and quadriceps m. of thigh, easy fixation after repair and suture, and early exercise of the knee joint. This novel method is simple and recommendable.

[Objective]To report the result of percutaneous biopsy in bone tumor and analyze the interrelated influence factors.[Method]A total of 171 patients were subjected to trocar puncture for biopsy of bone tumor in pre-operation,and the results of all cases were reviewed.[Result]Totally 155 cases in 171 cases showed positive outcome,the positive rate was 90.64%.The accuracy of 98 cases was comfirmed in 122 postoperative pathology diagnoses,and the acuracy rate was 80.33%.There were 110 cases of tumor.Biopsy sensitivity was 95.41%,and biopsy diagnostic particularity was 100%.[Conclusion]Percutaneous biopsy is both effective and valuable for the diagnosis of bone tumor in pre-operation,but the biopsy positive rate and accuracy,and postoperative complication was highly related to the operator cognitive level of bone tumor and meticulous manipulation and so on.