Liaoning University of Traditional Chinese Medicine has conducted pharmaceutical botany extracurricular activities such as the experiment skill competition,fieldwork,specimen preparation,resources investigation,database construction,pharmaceutical plants cultivating and participating in scientific research projects etc.These activities have improved the students' learning enthusiasm,strengthened the effect of first classroom teaching,and have played a positive role especially in improving students' experimental skills and cultivating their innovative thinking and abilities,and improving teachers' quality.

Objective To investigate the laboratory diagnosis of filamentous fungi which are rarely seen in clinical practice.Methods Five strains of fungi were isolated from clinical samples and initially identified by the morphological method.Total DNA of fungi was extracted and amplified by the PCR method using universal primers of ITS2-ITS4 gene, respectively.The PCR products were sequenced and the obtained sequences were then analyzed by the blastn program incorporated in NCBI.Results The five strains of fungi were diagnosed as Scedosporium apiospermum,Schizophyllum commune,Scopulariopsis brevicaulis,Rhizopus stolonifer,and Fusarium solani.Conclusion The laboratory diagnosis of filamentous fungi which rarely occur in clinical practice should integrate various methods,including morphological, microbiological,and molecular biological methods.

Objective : To explore season-specific association between ambient PM2.5 and outpatient visits for asthma in the urban areas of Ningbo. Methods : Based on the platform of population health information,we collected daily outpatient visits for asthma in four general hospitals in the urban areas of Ningbo from 2014 to 2016. We also collected data of meteorological indicators and air pollutants during the same period from local weather bureau and environmental monitoring center. Generalized additive model（GAM）was applied to examining the relationship and lag effects between ambient PM2.5 and outpatient visits for asthma in different seasons. Results : A total of 45 184 outpatients with asthma were reported from 2014 to 2016,with an average of 41.23 cases per day. The annual average concentration of PM2.5 in Ningbo was（43.12± 26.40）μg/m3 ,with the peak as（65.27± 34.51）μg/m3 in winter and the though as（27.34± 12.70）μg/m3 in summer. A 10 μg/m3 increase of PM2.5 were associated with 1.14%（95%CI：0.48%-1.81%）,2.40%（95%CI：0.22%-4.63%）and 1.37%（95%CI：0.28%-2.48%）increase of outpatient volume for asthma on the day in the whole year,summer and winter,respectively. In summer,males were at higher risk compared to females（P< 0.05）;in winter,people under 65 years old were at higher risk（P< 0.05）. Including NO2,the effect of PM2.5 on the excess risk of outpatient visits for asthma in summer was enhanced in two-pollutant models（P< 0.05）. Conclusion PM2.5 pollution might increase the outpatient volume for asthma in summer and winter. NO2 might have synergistic effect with PM2.5 on asthma.

OBJECTIVETo detect the expression of Nusap1 of surgical margins in hepatocellular carcinoma (HCC) and investigate its association with early tumor recurrence.

METHODSThe expression of Nusap1 in the surgical margins of HCC, which were histopathologically negative for tumor cells, was examined using immunohistochemistry in 61 HCC cases.

RESULTSFifteen of 21 (71.4%) cases with immunohistochemical positivity for Nusap1 expression in the surgical margins had early recurrence of HCC, a rate significantly higher than that in patients with negative Nusap1 expression (12/40, 30%) (P<0.05).

CONCLUSIONSNusap1 expression in the surgical margins of HCC is closely correlated to early postoperative recurrence and can serve as an indicator for predicting early recurrence of HCC.

Adolescent ; Adult ; Aged ; Carcinoma, Hepatocellular ; metabolism ; pathology ; surgery ; Female ; Humans ; Immunohistochemistry ; Liver Neoplasms ; metabolism ; pathology ; surgery ; Male ; Microtubule-Associated Proteins ; metabolism ; Middle Aged ; Neoplasm Recurrence, Local ; pathology ; Young Adult