Objective To investigate the role of cystatin C in hypertensive disorder complicating pregnancy.Methods Peripheral blood from 39 healthy pregnant women,23 patients with mild gestational hypertension ,25 patients with moderate gestational hypertension and 27 patients with severe gestational hypertension,CysC,BUN,UA and crea were determined .Resultss CysC ,BUN,UA and crea were higher in gestational hypertension group. CysC ,BUN,UA and crea were higher in severe gestational hypertension than mild and moderate gestational hypertension group.CysC had higher positive predictive ratio than UA for severe gestational hypertension.Conclusion CysC was a sensitive index to detect the early defection of kidney in severe gestational hypertension combining with other index such as BUN,UA and crea.

Objectives To localize the initiation site of the transcript driven by the cDNA corresponding to SARS - CoV 5 ' - UTR when it act as a promoter. Methods The plasmid pGI3 -5' - UTR, in which a reporter gene Luc was driven by the cDNA corresponding to SARS - CoV 5' -UTR, was transfected into HepG2 cells, and then the total RNA was extracted. Subsequently, the extracted total RNA was reverse - transcribed using a specific primer and subjected to two turns of semi - nested PCR. The PCR product was cloned into a T - vector for sequencing. Results A 440bps product was harvested by reverse - transcription and two turns of semi - nested PCR, and the 56th nucleotide of SARS - CoV 5 ' - UTR was found being the initiation site, followed downstream with a highly conserved transcription - regulating sequence (TRS). Conclusion The 56th nucleotide and its downstream TRS of SARS - CoV 5 ' - UTR is key roles in regulating gene transcription.

Objective To investigate the efficacy of Xuebijing injection on the septic shock and its impact on the patient's PCT and CRP.Methods According to the digital table,60 patients with septic shock were divided into two groups according to random number table methods.30 patients in the control group were only given conventional treatment,while the research group were used of Xuebijing injection in addition.The clinical efficacy was compared after treatment and the PCT and CRP levels were detected before and 7d after treatment.The days of hosoitalization were recorded.Results The total effective rate of the study group was 70％,significantly higher than 40％ of the control group's (x2 =5.45,P ＜ 0.05),and the study group's PCT and C RP levels were significantly lower than before (t =33.54,48.02,all P ＜ 0.05),after treatment 7d and the extent significantly greater than the control group (t =6.68,7.67,all P ＜ 0.05).Furthermore,the number of days of study group's hospitalization was significantly lower than that of control group(t =10.21,P ＜ 0.05).Conclusion Septic shock treated by Xuebijing injection has a significant effect as well as a greater impact on the PCT and CRP,which is worthy of clinical use.

OBJECTIVE To find out the situation of peri-operational lower respiratory tract infection in inpatients with congenital heart disease of left to right shunt and provide evidence for clinical prevention and treatment.METHODS Peri-operational lower respiratory tract nosocomial infection in 2 564 cases of congenital heart disease with left to right shunt during Jan 2003 to Dec 2005 was analyzed retrospectively.RESULTS The incidence rate of lower respiratory tract infection was 11.27% among 2 564 cases.The incidence rate was 15.51% in patients less than 2 years old.The most common pathogen was Pseudomonas aeruginosa(18.60%).The death rate in cases with lower respiratory tract nosocomial infection was higher than these non-infection cases.CONCLUSIONS Pulmonary congestion,less than 2 years old,mechanical ventilation and antibiotics abuse are main causes of lower respiratory tract nosocomial infection in patients with congenital heart disease with left to right shunt.Nosocomial infection can increase death rate.

The etiology of nasopharyngeal carcinoma is still not very clear.Epigenetics is proved to play an important role in the occurrence and development of nasopharyngeal carcinoma (NPC).Detection of epigenetics can serve as molecular index of NPC,and it is advantageous to the prognosis and disease of NPC.Different intervention measures in the epigenetics can be used as a new treatment of NPC,as well as the development of new NPC radiotherapy sensitization agent and new drugs.Study of epigenetics changes in nasopharyngeal carcinoma provides a new idea for the diagnosis and treatment in NPC and so on.

Based on the report of previous clinical study which showed cholinergic receptor antagonist scopolamine had antidepressant activity, this study was to investigate the antidepressant activity of scopolamine and explore its effective dose in mice, and to evaluate the effect of scopolamine on the central nervous system and learning/memory ability at its antidepressant effective dose. Tail suspension test, forced swimming test, step-down passive avoidance test and open field test were used to evaluate its effects on mice. Compared with the vehicle control group, single-dose administration of scopolamine (0.1-0.4 mg x kg(-1), ip) significantly decreased the immobility time (P < 0.01 or P < 0.001) in tail suspension test, and significantly decreased the immobility time (P < 0.001) in forced swimming test, but had no effect on the step-down latency and errors in step-down passive avoidance test. Scopolamine (0.1 and 0.2 mg x kg(-1), ip) had no influence on the locomotor activity in open field test, while at dose of 0.4 mg x kg(-1) significantly increase the locomotor activity. These results showed that scopolamine produced reliable antidepressant effect at doses of 0.1 and 0.2 mg x kg(-1), without impairment on learning and memory, as well as excitory or inhibitory effect on central nervous system in mice.

Aiming at the problems of cosmetic elective courses teaching,this article discusses some effective teaching methods such as adjusting teaching contents according to students'interest and putting comprehensive examination and evaluation into practice through optimizing teaching means.These measures have improved the teaching quality and effect of the elective course and meanwhile are beneficial attempts for the quality education

BACKGROUND:Human leukocyte antigen F-associated transcription factor 10 (FAT10) is highly expressed in many tumor cel s like colon cancer cel s, but its relationship with esophageal cancer is less reported. OBJECTIVE:To investigate the effects of siRNA interference technique on the invasion, apoptosis and the characteristics of EC9706 cel s, a human esophageal cancer cel line. METHODS:siRNA sequence was designed and synthesized according to the FAT10 mRNA encoding sequence, and the EC9706 cel s were transiently transfected. EC9706 cel s were divided into three groups:siRNA FAT10 group, negative control group, and blank control group. The expression levels of bcl-2 and FAT10 were detected by RT-PCR and western blot assay, respectively. Cel counting kit-8 assay was used to measure the proliferation of cel s in vitro. Flow cytometry was used to observe the changes of cel cycle, cel apoptosis and the expression of CD44+CD133+. TUNEL staining was used to detect the apoptosis of the cel s. Cel invasion in vitro was detected by Transwel invasion assay. RESULTS AND CONCLUSION:RT-PCR and western blot findings showed that compared with the negative control group and blank control group, the expression levels of bcl-2 and FAT10 mRNA and protein were significantly decreased in the siRNA FAT10 group (P<0.05);the percentage of CD44+CD133+cel s was decreased significantly (P<0.05);and significantly increased apoptosis rate, and decreased cel proliferation and invasion were also found in the siRNA FAT10 group (P<0.05). In conclusion, the specific silencing of FAT10 gene can reduce the invasion of esophageal cancer cel s, inhibit cel proliferation, reduce bcl-2 expression, and increase the apoptosis rate. Meanwhile, the proportion of CD44+CD133+cel s is decreased.

BACKGROUND:At present, heterologous serum as a medium is a common method for culture of bone marrow mesenchymal stem cells, but it is limited by the disease transmission between species, the potential immune rejection and even controversial ethic issues. This method is also contrary to the requirements of the Ministry of Health. Autologous platelet-rich plasma is a kind of whole blood extract, containing a variety of growth factors. OBJECTIVE:To explore the osteogenic differentiation of rabbit bone marrow mesenchymal stem cells cultured in autologous platelet rich plasma alternative to traditional heterogeneous serum-free culture. METHODS:8 mL bone marrow from the rabbit iliac crest was extracted and anti-coagulated with heparin, and then bone marrow mesenchymal stem cells were isolated using density gradient centrifugation. The cells were divided into autologous platelet rich plasma group (10%autologous platelet rich plasma) and fetal bovine serum group (10%fetal bovine serum). At the passage 4, the cells in the two groups were respectively subdivided into experimental and control groups. Experimental groups were subjected to osteogenic induction, while no change was done in the control groups. cellproliferation was determined by using growth curves;the activity of alkaline phosphatase was detected to adjust the osteogenic differentiation of cells in different groups. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells at different generations al showed good proliferation. At 12 days of autologous platelet rich plasma culture, the activity of alkaline phosphatase in the passage 4 cells in the experimental group was significantly higher than that in the control group;while the activity of alkaline phosphatase in the autologous platelet rich plasma group was significantly higher than that in the fetal bovine serum group (P<0.01). These findings indicate that autologous platelet rich plasma as a substitute of xenogeneic serum for culture of bone marrow mesenchymal stem cells is a method characterized as safe and reliable, simple operation, high-purity active induction.

Objective To observe effects of BMP-4 gene combined with TGF-β1 in repairing rabbit femur defect resulted from firearm wound. Methods The femur defect was made with firearm steel ball. Plasmid encoded BMP-4 gene identified in vitro and TGF-β1 were injected into the tissue of upper, lower and central parts of the defects at the second week after wound. The mRNA and protein expressions of BMP-4 in vivo were detected by real time-polymerase chain reaction (RT-PCR) and Western blot. Activity of alkaline phosphatase (ALP) and calcium content were measured for evaluating osteogenetic ability. The process and quality of osteogenesis were determined by pathological and X-ray examinations. Results mRNA and protein of BMP-4 could continually express for six weeks in vivo after injection. Activity of ALP in the experimental group was increased to ( 13.17 ±0.51 ) U/100 ml at the 8th week, which was significantly higher than (8.77 ± 0.44) U/100 ml in the control group, indicating that osteogenetic ability was markedly enhanced, which accorded with determination of calcium content. Pathological observation and X-ray proved prominent improvement of osteogenesis, with a shorter time and better quality, in the experimental group. Conclusion BMP-4/TGF-β1 can promote repair of firearm femur defect.