Objective To explore a practicable method of serial sections of whole CNS in the rat, in which both neurons and nerve fibers were showed at the same section. Methods After perfused fixation and quick freezing, the tissues of whole CNS were cut into serial sections with cryostat followed by hematoxylin stain. Results The nerve fibers were stained in blue color while the neurons were showed black-blue and the background was light-yellow.Conclusion Our method was feasible to show both neurons and nerve fibers at the same section with quick freezing, freezing serial sectioning and improved hematoxylin staining.

Objective To study the mechanism of male infertility. Methods Forty sperms from 8 normal males as control and 60 sperms from 12 males with infertility were observed by SEM.The ultrastructure of acrosomes was analyzed and compared. Results Spermatozoa with obscure ringed rips in infertile group acounted for 66.7%(40/60),serrate 65.0%(39/60);but in control group,the proportions were 10.0 % (4/40) and 5.0%(2/40),respectively.The ratios of acrosome to head in infertile group were ( 70.0 ?8.0)%,greater than controls (57.7?6.4)%.The ratios of acrosome membrane which was not intact were 70.0%(42/60) in infertile group and 22.5%(9/40) in control group.Acrosomes with rough surface were 68.3%(41/60) and 20.0%(8/40) respectively in the 2 groups;Acrosomes with particles adhesive to the surface were 65.0%(39/60) and 27.5%(11/40) respectively. Conclusions Abnormal acrosome structure is one of the causes of male infertility.

The treatments for ankylosing spondylitis (AS) are still not effective, which partially due to its unclear patho?genesis. Interleukin (IL)-23/IL-17 axis is a significant immunologic pathway which has attracted much attention in the past few years. This article reviewed the role of IL-23/IL-17 axis in genetic and immunological pathogenesis of AS, and intro?duced the recent progresses in treatments of AS using anti-IL-23 or anti-IL-17 antibody. The important role of IL-23/IL-17 axis in the pathogenesis and treatment of AS indicates its potential use as a new therapeutic target.

Objective To investigate the efficacy of flupentixol and melitracen(Deanxit) in treatment of non-erosive reflux disease(NERD) and non-responding to proton pump inhibitor. Methods Fifty eight NERD pa-tients were randomly divided into two groups, the treatment group (n = 29) and control group (n = 29). Patients in control group were given esomeprazole 40 mg qd. Patients in the treatment group were given the same drug plus Deanxit 1 Tab qd. The clinical effect was evaluated and scored by Hemihon Anxiety Rating Scale (HAMA) after 4 weeks. Results The total efficacy rate of the treatment group was higher than control group (P < 0.01),and HAMA was lower in the treatment group than in control group (P < 0.01). Conclusions Adjuvant treatment of Deanxit combined with esomeprazole is effective to NERD patients who did not respond to proton pump inhibitor, can greatly improve anxious disorders.

ObjectiveTo investigate the risk factors of early postoperative complications after pancreaticoduodenectomy.MethodsThe clinical data of 159 patients undergoing pancreaticoduodenectomy from March 2005 to October 2010 was analyzed retrospectively.The related factors of early postoperative complications were analyzed.ResultsThe incidence rate of early postoperative complications was 30.8％ (49/159).Univariate analysis showed that preoperative fasting blood glucose level,total serum bilirubin level,serum albumin level,high density lipoproteins level and intraoperative bleeding were closely correlated with early postoperative complications in patients undergoing pancreaticoduodenectomy (P ＜ 0.05),but gender,age,preoperative jaundice,history of diabetes mellitus,operation time and whether portal vein reconstruction were no significantly associated with early postoperative complications in patients undergoing pancreaticoduodenectomy (P ＞ 0.05).Multivariate analysis showed that preoperative fasting blood glucose level( ≥6.1 mmol/L),total serum bilirubin level ( ≥ 171 μ mol/L) and serum albumin level( ＜ 35 g/L) were the important independent risk factors of early postoperative complications in patients undergoing pancreaticoduodenectomy.ConclusionThe risk factors of pancreaticoduodenectomy should be fully recognized and dealt with promptly.

The endothelial cells activated by tumor cells are genetically stable and regress easily. Moreover, at the surface there are specific markers, which are easily accessible by system administration. This report review the studies of tumor treatment targeting at the activated endothelial cells.

Objective To explore the clinical value of using heparin in DIC caused by Tsutsugamushi.Methods 27 cases of Tsutsugamushi with DIC were divided into two groups. The heparin treatment group(n = 15) and non-heparin treatment group(n = 12). The two groups were compared and analyzed. All the cases were diagnosed definitely depended on the "Tsutsugamushi-Disease-Diagnosis Standard".Results The group which use heparin improved in rectifying shock,terminating heorrhage,restoring the time of lab index of DIC,rating of MOF and death. Compared with the control group, the difference had statistical significance(P

Objective To investigate the effects of propofol on ketamine-induced HSP 70 mRNA and protein expression in the rat posterior cingulate cortex and to explore the possibility of using propofol to prevent or treat ketamine-induced psychotomimetic effects and neuronal damage. Methods Thirty male Wistar rats weighing 250-300 g were randomly divided into 5 groups with 6 animals in each group: group 1 received normal saline intraperitoneally ip (NS); group 2 received ketamine 100 mg? kg-1 ip (K); group 3 received propofol 100 mg ? kg-1 ip (P); group 4 received propofol 50 mg?kg-1 + ketamine 100 mg?kg-1 ip (P1 K) and group 5 received propofol 100 mg?kg + ketamine 100 mg?kg-1 ip (P2K) . In group 4 and 5 the interval between propofol and ketamine administration was 15 min. Twenty-four hours after ketamine and/or propofol administration, the animals were decapitated and brain was removed. HSP 70 mRNA expression in the posterior cingulate cortex was detected by using semi-quantitative RT-PCR technique; HSP 70 protein expression in posterior cingulated cortex was determined by immuno-histochemical method. Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner. Conclusions Propofol pretreatment can significantly inhibit ketamine-induced HSP 70 mRNA and protein expression in the posterior cingulated cortex. It may be one of the mechanisms of inhibition of ketamine-induced psychotomimetic effect and neuronal damage by propofol.

Objective To study the effects of intrathecally administered morphine and ketamine on nitric oxide synthase (NOS) activity and nitric oxide content in the spinal cord.Methods Thirty-two male Wistar rats weighing 220-260 g were anesthetized with intraperitoneal chloral hydrate 300 mg?kg-1 . A catheter was implanted in subarachnoid space at lumbal region. Sciatic constriction injury (SCI) was produced by loose ligation of right sciatic nerve trunk with 4-0 cutgut. On the 4th postoperative day the animals were randomly divided into four groups of 8 animals :(1) control group (C); (2) morphine group (M);(3) ketamine group (K) and morphine-ketamine group (KM) . Morphine 20 ?g / ketamine 20 ?g / morphine 10 ?g + ketamine 10 ?g were injected intrathecally every day for 7 consecutive days in group M, K and KM. In control group normal saline was injected intrathecally instead of morphine and / or ketamine. The withdrawal latenvies to radiant heat focused on plantar surface were measured as radiant heat threshold before intrathecal administration of the analgesic (baseline) and 30 min after intrathecal administration of ketamine and / or morphine every day for 7consecutive days. The percentage of maximal possible effect ( MPE % ) was calculated : MPE % = (latency after intrathecal administration-baseline latency) / (radiant heat cut-off time-baseline latency) X 100% . After 7 days of intrathecal administration the animals were decapitated and the spinal cord was immediately removed and the lumbal spinal cord was dissected on ice. NOS activity and nitric oxide ( NO) content were measured by spectrophotometry. Results MPE % was significantly higher in group M and KM than in group C and K ( P

According to the actual situation of taking part in the earthquake relief mission followed by the mobile field hospital,the characteristics of medical support mission and the main problems in the use of medical equipment of the mobile field hospital are summarized.Aimed at the problems,equipments are suggested to be added especially used for surgery,first aid,blood transfusion and some special subjects,and improvement should be made on the equipment of ultrasonic diagnosis,inspection and X-ray examination.Finally,the necessary sorts and quantity of the medical equipments needed in the mission are listed.