Objective To study the feasibility of adult stem cell therapy in motor neuron disease. Methods From May 2008 to March 2010, twenty patients with motor neuron disease were treated with adult stem cell. The patients were divided into two groups with umbilical cord mesenchymal stem cell in 14 cases (group A)and autologous Hematopoietic stem cell in 6 cases( group B). 4 ml stem cell ( ＞ 1.0 × 107) were given through subarachnoid injection. 1.0 × 107 cell were injected each time, and rehabilitation were performed simultaneously. Results Seven patients have improved in the dysphagia, bucking, continuity of language and thinking and reaction after 2 weeks' treatment. Seven patients improved remarkably in myodynamia( from Ⅲ to Ⅳ ). There was no effect observed in 6 patients. Three patients died during the follow up for 3 - 6 months. Three patients were followed up for 18 to 24 months and their condition did not getting worse. Conclusion Adult stem cell transplantation may be a safe and practical new ways to treat motor neuron disease.

Objective To study the effects and mechanism of different administrations of atorvastatin on myocardial ischemia/reperfusion (MI/R) in rats.Methods A total of 160 male SD rats were randomly divided into five groups:sham group,MI/R group,atorvastatin of conventional dose (MI/R + N) group,atorvastatin of preoperative signal loading dose (MI/R+SL) group,and atorvastatin of preoperative continuous loading dose (MI/ R+ML) group.MI/R model was established in the rats.Myocardial infarction size was detected by Evans blue/ TTC staining.The activity of ATPase of cardiac muscle and the levels of serum IL-6 and TNF-α were detected by ELISA.The level of LVEF％ was detected by small animal ultrasound.Results Compared with MI/R+N group,MI/R+ SL and MI/R+ ML groups had significantly smaller myocardial infarction size (P＜0.05),higher activity of ATPase (P＜0.05),lower levels of serum IL-6 andTNF-α (P＜0.05),and more advancedLVEF％ (P＜0.05).However,MI/R+SL group and MI/R+ML group did not differ significantly in the above-mentioned parameters.Conclusion Atorvastatin of loading dose might alleviate MI/R injury by improving ATP metabolism of cardiac muscle and reducing abnormal expressions of inflammation factors.Meanwhile,the administration of preoperative continuous loading dose and preoperative signal loading dose of atorvastatin may not differ in protecting against MI/R injury.

Objective To investigate the relationship between mild cognitive impairment and quality of life in hemodialysis patients with diabetic nephropathy.Methods One hundred and twenty-five hemodialysis patients with diabetic nephropathy were selected in Affiliated Hospital of North China University of Science and Technology from December 2014 to December 2016,and were divided into two groups according to mild cognitive impairment,including 73 patients without mild cognitive impairment as control group,and 52 patients with mild cognitive impairment as observation group.Patients received Montreal cognitive assessment scale and kidney disease quality of life scale.Patients received clinical index detection.Results Montreal cognitive assessment scale(visual space and execution(3.0±0.7) points,naming(2.4±0.3) points,attention(4.3±0.7) points,language(2.1±0.4) points,abstraction(1.1±0.2) points,delayed recall(1.6±0.3) points,orientation(5.4±0.5) points) score,kidney disease quality of life scale(physical(70.3±3.5) points,physical constraints due to work and life(14.6±2.8) points,pain(56.4±7.1) points,general health(36.2±3.4) points,emotional status(51.3±2.6) points,social function(52.7±3.4) points,mental status(43.2±3.6) points,emotional constraints due to work and life(41.3±2.8) points,symptoms(73.6±2.1) points,work status(20.3±1.6) points,cognitive function(72.7±1.8) points,nephropathy effect(49.1±3.2) points,sexual function(53.2±4.0) points,burden of nephropathy(21.3±2.6) points,sleep quality(70.1±1.5) points,social quality(43.3±1.9) points,social support(71.2±2.3) points) score in observation group were lower than control group((4.4±0.8) points,(3.0±0.0) points,(5.4±0.5) points,(2.7±0.3) points,(1.8±0.4) points,(3.4±0.6) points,(6.0±0.0) points),((78.6±4.1) points,(25.7±3.6) points,(69.8±4.2) points,(41.0±2.7) points,(58.5±3.0) points,(61.2±3.8) points,(53.4±2.8) points,(46.5±2.4) points,(82.9±3.2) points,(24.2±2.7) points,(81.6±2.3) points,(65.3±4.0) points,(62.8±3.9) points,(35.7±2.8) points,(79.3±2.0) points,(59.7±2.4) points,(82.4±2.6) points)(t=7.667,5.502,5.558,5.857,9.364,14.250,4.112,4.038,10.603,5.321,4.326,4.403,4.613,5.361,4.262,4.315,4.923,4.224,6.265,4.804,6.712,4.316,6.787,4.573,all P<0.05).Conclusion Hemodialysis patients with diabetic nephropathy has mild cognitive impairment,while quality of life is low.

Objective To evaluate the diagnosis and treatment of urinary tract endometriosis.Methods Retrospective review of 10 female cases of urinary tract endometriosis was carried out. All cases age was from 28-49, and the average age was 39-year-old. The course of this disease was from 6 months to 3 years. Four of 10 cases were bladder endometriosis. The clinical presentations included the urgency, frequency, pain at micturition and lower abdomen pain during menstruation, gross hematuria coinciding with menstruation 1 case. B-ultrasound and CT showed the mass of bladder from 2. 0 -3.5 cm but were not specific. Four of 6 cases ureteral endometriosis were the left side and 2 cases were the right side. This clinical presentation included non-specific flank or abdomenal discomfort in 4 cases, intermittent gross hematuria in 1 case and 1 case renal hydronephrosis was found incidentally by B-ultrasound. B-ultrasound indicated unilateral upper urinary tract dilation and hydrops in all cases,with pyelic separation from 2.0-4.5 cm and ureteral separation from 1-2 cm. CT indicated stenosis of the lower ureter in 5 cases, ureter tumor in 1 case. Results All cases were treated surgically.Partial cystectomy were performed in 4 cases of bladder endometriosis, of which, 1 case bilateral oophorectomy and hysterectomy. Five cases were performed ureteral segmental resection, of which, 3 ureterocystostomy and 2 terminoterminal anastomosis. 1 case was performed radical nephrectomy and ureterectomy. Postoperative pathological findings confirmed the diagnosis of endometriosis. Postoperative oral hormone therapy was given to 9 cases for 6-12 months. All cases were followed for 12-60months. 2 cases ureteral endometriosis had recurrent hydronephrosis in 18-24 months. The 2 cases received ureteral stent and cured by oral hormone therapy or goserelin subcutaneous injection for 3 months. Conclusions Urinary tract endometriosis usually shows non-specific symptoms. The diagnosis can be missed on both clinical examination and preoperative work-up. Surgical treatment is effective and adjuvant hormone therapy is often recommended to prevent the recurrence.

The psychiatric problem has become a global public health issue. The model of mental health service advocate that“Treatment of disease occurred in the hospital, but rehabilitation and management in the community”. This brings great challenge to the community health service center. By comparing the domestic and foreign mental health service system, this paper discusses the necessity of carrying out the standardized training of general practitioners in psychiatry and psychology.

Objective Discuss the interference of injection cefotiam on vanadate oxidation method and dry chemical method assay total bilirubin .Methods Collected 60 examples ,include total bilirubin concentration 20 examples less than 20 μmol/L ,20 examples between 150-220 μmol/L and 20 examples between 350-410 μmol/L ,add an equal volume of various concentrations of cefotiam in each case ,formulated into cefotiam final concentrations of 300 ,150 ,75 mg/L of serum samples as the test group ,add an equal volume of water in each serum samples as the control group ,determine all the samples total bilirubin concentration respectively by vanadate oxidation method and dry chemical method ,compared the interference of cefotiam on determined total bilirubin by two method ,analyze the data by SPSS13 .0 .Results Determined total bilirubin by dry chemical method ,the test group higher than the control group ,the difference was statistically significant(P<0 .05) ,at the same total bilirubin levels ,with cefotiam concentrations decreased ,increased rate of total bilirubin concentration were decreased in the experimental group .Determined total bilirubin by vanadate oxidation method ,when the total bilirubin concentration between 150 -220 μmol/L ,the test group was higher than the control group ,the difference was statistically significant(P<0 .05) .Conclusion Interference of injection cefotiam on determined to‐tal bilirubin by dry chemical method is strong ,and with the drug concentration increased ,effect is more obvious ,but determination of total bilirubin by vanadate oxidation method has almost no effect .

Objective To evaluate the influence of rectal washout on the prognosis of rectal cancer after anterior resection.Methods Data of 144 patients with rectal cancer undergoing anterior resection at Chinese Academy of Medical Science Cancer Hospital between May 2006 and November 2007 were reviewed.69 patients received rectal washout and 75 patients did not.The incidences of recurrence and 5-year disease-free survival rates were compared.Data were analyzed by SPSS 13.0 software packet,using x2 and t inspection.Kaplan-Meier method was used to calculate postoperative survival rate.Results Washing sample was positive for malignant cells in 55 patients (79.7％).The incidences of recurrence were 4.6％ and 6.7％ in washout group and no-washout group respectively (x2 =0.368,P =0.721).The five-year disease-free survival rate was 79.7％ in washout group and 74.7％ in no-washout group(x2 =0.517,P =0.553).Conclusions Free malignant cells were ubiquitous in rectal lumen during anterior resection,and there was a high positive rate of cytological examine for washout sample.Rectal washout may reduce local recurrence and prolong patients' survival.

Objective To summarize CT features , benign and malignant differential main points of the tumors of germ cell origin in the mediastinum.Methods 32 cases with pathologically confirmed the tumors of germ cell origin in the mediastinum were retrospectively analysed.Results Of 32 cases,26 were benign tumors,including cyst mass in 10,solid mass in 9 and cyst-solid mass in 7.6 cases were malignant tumors, including seminoma in 1 ,endodermal sinus tumor or yolk sac tumor in 1,embryo cell casinoma in 1 and malignant teratoma in 3 .Typical benign teratoid tumors appeared as water to soft tissue density masses,with defined margin,fat or calcification inside the tumors,around tissues were compresed by tumors,the malignant tumors appeared as lobular or irregular masses and around organ invaded.Conclusion CT is a reliable method for the diagnosis of the tumors of germ cell origin in the mediastinum accurately.Malignant tumors of germ cell origin in the mediastinum are difficult differentiation from other mediastinal malignant tumors.

Objective To explore the imaging features of primary bone tumors and tumor-like lesions of the skull,so that to improve the diagnostic accuracy of the disease.Methods 47cases with bone tumors and tumor-like lesions of the skull confirmed by pathology or typical imaging manifestations were retrospectively analysed.Results Of 47 cases,there were benign tumors in 22 cases,malignant tumors in 6 cases and tumor-like lesions in 19 cases.Benign tumors included osteoma of 11cases,epidermoid tumor and cyst of 4 cases,osteochondroma and neurofibromatosis of 2 cases respectively,carvernous angioma ,osteoid osteoma,and benign fibrohistiocytoma of 1case respectively.Malignant tumors included multiple myeloma and angiosarcoma of 2 cases respectively,Ewing’s tumor and osteosarcoma each and all in 1 case .Tumor-like lesions included fibrodysplasis of bone in 8 cases, Langerhans’ cell histiocytosis in 7 cases ,brown tumor and deformed osteitis each and all in 2 cases.Conclusion Primary bone tumor and tumor-like lesions of the skull are of a great variety,each one has its own imaging characteristics,the diagnosis of them should be combined with clinic and pathologic data.

Objective To explore the effects of electroacupuncture in different time on the variations of fractional anisotropy (FA) mean value in diffusion tensor tractography (DTT) in spinal cord injured (SCI) rats. Methods 48 Sprague-Dawley rats were performed with Allen's method by NYU blow device, resulting in T10-11 SCI. They were divided into groups according to random table. Control group (n=16) received no treatment. Experimental group (Group A, n=32) received electric stimulation in the scalp surface projection area of motor area and local electric stimulation at damaged site. Group A was divided into Group A1 (n=16) and Group A2 (n=16) again. Group A1 received electric stimulation 3 days after SCI, while Group A2 received electric stimulation 2 weeks after SCI. They were assessed with Basso-Beattie-Bresnahan (BBB) scores and routine MRI and diffusion tensor imaging (DTI). Results There was no significant difference in BBB score among all the groups at the first week (P>0.05). BBB scores of the Groups A1 and A2 were significantly different from the control group at the second and fourth week (P<0.001), and the Groups A1 from A2 (P<0.001). 2~4 hours and 3 days after modeling, there was no significant difference in the FA value among the groups (P>0.05). There was significant difference among the Groups A1, A2 and the control group at the fourth week (P<0.001). Group A1 was better than Group A2 (P<0.001). DTT images of all the groups were successful reconstructed, the Groups A1 and A2 repaired better than the control group. Conclusion The earlier electroacupuncture is taken after spinal cord injury, the better the outcome is.