The 340 blood samples from Guangdone area were detected by ultra thin poly-acrylamide gel isoelectric focusing for EAP phenotypes.The phenotypes were20 EAP A,119 EAP BA,201 EAP B.The gene frequencies of EAP were as foll- ows p~(?)0. 2338,p~(?)0. 7662. The 110 bloodstain samples of the cloth kept at roomtemperature for 7 weeks could be phenotyped correctly,The 21 bloodstain samp-les on the porcelain plate kept at room temperature for 9 weeks could be phen-otyped correctly.When the blood volume of bloodstain was equal to or over5?l EAP could be phenotyped.6 out of 20 mouldy bloodstains,the EAP BAphenotype were changed to EAP B.In blind trial,20 bloodstain samples kept atroom temperature for 7 weeds could be phenotped correctly.

The phenotype distribution of human red cell glyoxalase I of a Han population in Guangzhou area was studied using mixed starch/agarose gel electrophoresis. The phenotype frequencies were: GLOI 1-1 2.57％; GLOI 2-1 29.17％; and GLOI 2-2 68.26％. The gene frequencies were: GLOI~1 0.1716; GLOI~2 0,8284. The phenotyping of GLOI was carried out satisfactorily in 35 bloodstains kept in room temperature for 20 days in 7 bloodstains stored in 4℃ for 105 days exposed in sunshine for 8 hours, as well as kept outdoor overnight, and in 10 putrefactive bloodstains kept in room temperature for 9 days.The GLOI were destroyed in 6 of 7 bloodstains washed by runing water for 2 hours.

To investigate haplotype distribution of the Y-chromosome STRs (DYS19, DYS390 and DYS389 locus) in Han population in Guangdong area. The STRs were typed by poplymerase chain reaction followed by discontinuous PAGE system. Among 130 unrelated males, 81 different haplotypes were observed, 52 out of them were found only one time. The haplotype genetic diversity, discrimination power and non-father exclusion chance are 0. 9989, 0. 9824, 0. 9824, respectively. The high informative haplotypes make these STRs useful for the forensic individual identification and paternity testing.

To evaluate species specificity of 20 STRs loci used in our lab. Human DNA and 10 different kinds of common animals' DNA were amplified separately. The PCR products were analysed with PAGE. There are amplified products in DYS388, DYS389, DYS390, D7S809, D13S631 loci of human 'DNA, but not of 10 different kinds of animal DNA tested. There were amplified products in TH01, vWA, AR, CD4, FABP loci for human and animals' DNA, but the lengths of amplified fragment were distinctly different between human and animals. There were products of FES, D12S391, CSF1PO, D19S253, D21S11, FGA, DYS19 loci for human and animals DNA, but the product lengths were not different between human and animals. There were PCR products in SE33 and D11S554 loci for human and animals, their amplified fragment lengths were within the same electrophoretic field. 10 STR loci, such as TH01 etc, were highly specific for human being and the amplified fragments lengths of 8 STR loci, such as FES etc, were different between human and animal tested.

By analysing the 300 cases of paternity test in our lab in the recent year, we have demonstrated that mutation rates in STR loci are very high. The experimental steps include extracting DNA by chelex 100, amplification of DNA, polyacrylamide gel electrophoresis, silver staining, DNA typing by standard samples. The STR mutations in 11 cases among 300 paternity test cases were found, induding D11S554 locus6 cases, D19S253 locus2 cases, SE33 locuslcase, D12S391 locus 1 case, D13S631 locus 1 case. The results indicated that high mutation rates in STR loci should be considered when STR genotyping are applied to paternity test.

To establish a calculation method of paternity probability in cases of absence of mother. Calculating the accumulated non-father exclusion probability of multiple polymorphic DNA loci was performed. The results showed that in cases of inheritance with Mendelian Law, through testing 8 or more polymorphic DNA loci, the paternity probability may reach 0. 9990 or more. In cases of paternity exclusion, exclusion 3 or more than 3 loci were required. For the purpose of identifying paternity in cases of absence of mother, more than 8 polymorphic DNA loci must be tested. The paternity probability must be more than 0. 9990 in cases of paternity inclusion and in cases of paternity exclusion, 3 or more loci of exclusion is needed.

[Objective] To screen and identify the new Y-STR loci from the Y chromosome and examine the polymorphism of these Y-STR loci. [Method] To seek and locate the position of 5 Y-STR loci, including DYS709, DYS720, DYS721, DYS722, and DYS723, and perform sequencing of these 5 Y-STR loci. Then to investigate the polymorphism in unrelated Chinese Han males. [Results] Five Y-STR loci were identified from Y chromosome sequence. By scrutinizing the physical position on Y chromosome of previously reported Y-STRs, we found that three loci were novel and two loci overlapped with two loci published only online. All loci could be male-specifically amplified with a product size ranging from 185 bp to 278 bp. After 108 males of the Chinese Han Population (Guangzhou) were examined, we found 5 DYS709, 11 DYS720 alleles, 4 DYS721 alleles, 6 DYS722 alleles, and 6 DYS723 alleles. A total of 95 haplotypes were identified, 84 of which were unique, and with a haplotype diversity of 0.997 2±0.001 2(HD±SE). [Conclusion] This set of Y-STRs can be used as Y chromosome genetic makers in related fields.

BACKGROUND: Long-term radioactive ray accumulation leads to surrounding tissues fibrosis accompanied by limitation of mouth-opening, which seriously affects life quality of tumor patients. OBJECTIVE: To observe pathological change of soft tissues in rats with radiation masseter injury and the level of transforming growth factor β1 (TGF-β1) mRNA expression in vivo.METHODS: A total of 30 Wistar rats were randomly divided into control group (n=10) and radiated group (n=20). Radiated group were radiated with linear accelerator at a dose of 40 Gy. The pathological changes of vessels were observed under light microscope and electron microscope and expression of TGF-β1 was detected by RT-PCR. RESULTS AND CONCLUSION: The microvessel density of radiated group was obviously decreased than that of the control group (P < 0.01), but the mRNA expression of was higher than that in the control group (P < 0.01). It suggests that radiated injury can induce fibrosis to repair the radiated injury.

Objective To develop a database management system specially applied for medical equipment maintenance administration, Methods Under PowerBuiider/SQL Server development environment, the C/S mode system structure was confirmed and the system function modules were designed. Results The application of C/S mode structure was coincident with the division-cooperation work flow in large and medium hospitals, and the design of function modules met the demand of information collection and utilization of medical equipment maintenance.Conclusion The database management system can preserve the information of medical equipment maintenance effectively and facilitate its usage. Moreover, many statistical indexes about the maintenance administration are available in this system.

10.3969/j.issn.2095-4344.2013.24.021