Objective To explore the relationship of urinary iodine level between 8-10 year-old children and adults under the same living conditions in coastal and mountain areas in Fujian Province.Methods Representative coastal and mountainous rural areas (Xindian Town Xiang'an District Xiamen City and Yongfu Town Zhangping District Longyan City) were selected as survey sites.Morning fasting urine samples from local 8-10 year-old children and their parents and a random urine sample in the morning from these children were collected.Urinary iodine was determined using the arsenic cerium catalytic spectrophotometric method (WS/T 107-2006).Results The median urinary iodine of fasting urine samples which were collected from 147 8-10 year-old children in coastal rural areas (71 boys and 76 girls) was 163.5 μg/L (boys 161.0 μg/L,girls 170.9 μg/L),and the median urinary iodine of random urine samples was 190.8 μg/L (boys 208.7 μg/L,girls 177.0 μg/L),and the difference was not statistically significant between boys and girls (Z =-0.376,-0.678,all P ＞ 0.05).The median urinary iodine of fasting urine samples which were collected from 116 8-10 year-old children in mountain rural areas (66 boys and 50 girls) was 171.8 μg/L (boys 169.5 μg/L,girls 173.8 μg/L),and the median urinary iodine of random urine samples was 210.8 μg/L (boys 233.2 μg/L,girls 203.4 μg/L),and the difference was not statistically significant between boys and girls (Z =-0.413,-0.881,all P ＞ 0.05).The median urinary iodine of random urine samples which were collected from 8-10 year-old children in mountain rural areas was significantly higher than that of the morning fasting urine samples (Z =-2.789,P ＜ 0.05).The median urinary iodine of children's fathers in coastal rural areas was 177.0 μg/L,and the median urinary iodine of their mothers was 181.7 μg/L.There was no significant difference between coastal rural children and their parents (Z =-0.387,-0.658,all P ＞ 0.05),and there was also no significant difference between fathers and mothers (Z =-0.014,P ＞ 0.05).The median urinary iodine of children's fathers in mountain rural areas was 227.3 μg/L,and the median urinary iodine of their mothers was 237.4 μg/L There was a significant difference between mountain rural children and their parents (Z =-4.176,-4.373,all P ＜ 0.05),and there was no significant difference between fathers and mothers (Z =-0.291,P ＞ 0.05).Conclusions Urinary iodine level of school-age children does not reflect the actual urinary iodine level of adults.There is a difference between urinary iodine level of random urine sample and morning fasting urine sample.Attention should be paid to this difference when monitoring iodine nutrition in population.

Aim To observe the expression of MRTF-A in rat glomerular mesangial cells(GMCs) induced by advanced glycation end products(AGEs) and its effect on ICAM-1 and FN;to explore whether MRTF-A is involved in the process of diabetic nephropathy by affecting NF-κB pathway.Methods Under the condition of AGEs, CCG-1423 and anti-MRTF-A small interfering RNA were used to knock down MRTF-A and MRTF-A plasmid was used to activatt MRTF-A, The expression level of MRTF-A, ICAM-1, FN and p65 in nucleus were detected by Western blot.Results The protein expressions of MRTF-A was increased in AGEs-induced GMCs.The expressions of FN and ICAM-1 and p65 in nucleus were downregulated by knocking down MRTF-A.However, the expressions of FN, ICAM-1 and p65 in nucleus were upregulated by overexpressing MRTF-A.Conclusions AGEs can upregulate the expression of MRTF-A in GMCs, and MRTF-A mediates the protein expressions of FN and ICAM-1 by affecting NF-κB signaling pathway in AGEs-induced GMCs.

Objective Using Beckman assay kit to establish women thyroid function indicators reference interval during pregnancy in Fujian Province.Methods Two iodine sufficient areas were selected in Fujian randomly,and the women who did pregnancy test in local hospital were investigated.A questionnaire study and physical examination were conducted and women's serum samples were collected to detect the thyroid function [thyroid stimulating hormone (TSH),free triiodothyronine (FT3),free thyroxine (FT4),total triiodothyronine (TT3),and total thyroxine (TT4)] using Beckman chemiluminescence analyzer access-2 and assay kit.Reference interval was shown with median (M) and P2.5-P97.5.At the same time,the newly established reference interval was used to evaluate the thyroid function of 830 pregnant women.Result A total of 455 pregnant women were detected (early,middle and late pregnancies were 146,157 and 152,respectively).Most of the thyroid function indexes presented skewed distribution.The thyroid function index reference intervals in early,middle and late pregnancies were TSH:0.80 (0.02-2.96),1.13 (0.07-3.56),1.18 (0.09-3.76) mU/L;FT3:4.28 (3.30-5.76),3.74 (2.78-4.83),3.63 (2.81-4.56) pmol/L;FT4:11.78 (8.66-19.68),9.34 (7.03-12.76),8.55 (6.20-11.73) pmol/L;TT3:1.91 (1.09-2.95),2.00 (0.90-2.96),1.98 (0.91-3.02) nmol/L;and T4:123.00 (79.62-194.85),115.42 (58.78-175.85),117.43 (58.29-192.00) nmol/L.The thyroid dysfunction rate diag,nosed by our reference interval was 22.0％ (183/830),whilch was lower than 28.0％ (232/830) diagnosed by American Thyroid Association (ATA) reference interval (x2 =7.714,P ＜ 0.05).Conclusions Women thyroid function indicators reference interval during pregnancy in Fujian Province is successfully established.The study shows that each area and laboratory should establish the thyroid hormone reference interval of pregnant women for diagnosis and treatment of thyroid disease.

Objective:To construct evaluation index system for the communication effectiveness of nursing WeChat public accounts in hospital, and to provide a scientific and systematic evaluation tool for the communication effectiveness of nursing WeChat public accounts in hospital.Methods:Based on Lasswell′s "5W" communication process model, the evaluation index system for the communication effectiveness of nursing WeChat public accounts in hospital was constructed by applying Delphi expert consultation method and analytic hierarchy process.Results:The the evaluation index system for the communication effect of nursing WeChat public accounts in hospital consists of 5 first-level index, which contained communicator, communication content, communication media, communication audience, communication environment, and 12 second-level index, which contained professional quality, function setting, public trust, using experience, internal environment, etc, and 52 third-level index.Conclusions:The evaluation index system for the communication effectiveness of nursing WeChat public accounts in hospital could provide nursing managers an systematic evaluation tool. It could promote the construction and development of nursing WeChat public accounts and ensure that patients receive the health information which were scientific, authoritative and standard.

Objective To analyze the correlation between gasdermin E(GSDME)and the occurrence and development of cervical cancer.Methods HPA database and GEPIA2 online website were used to analyze the differential expression of GSDME gene and protein between cervical cancer tissues and normal cervical tissues.GEPIA2 and KM plotter online website was used to analyze the correlation be-tween GSDME gene expression and the pathological staging,survival and prognosis of cervical cancer.cBioPortal online website was used to analyze the GSDME gene mutation in cervical cancer tissues.TIMER2 online website and R-package were used to analyze the correla-tion between GSDME and immune infiltration in cervical cancer tissues.Gene set enrichment analysis was used to analyze the biological function of GSDME in development of cervical cancer.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to verify the transcription and translation of GSDME in cervical cancer cell lines.Results Compared with normal cervical tis-sues,the expression of GSDME mRNA and protein in cervical cancer tissues was lower(P＜0.05).Survival analysis showed that the high expression of GSDME gene may be associated with poor prognosis of cervical cancer patients.0.36％of GSDME amplification muta-tion was found in cervical squamous cell carcinoma.There was significant negative correlation between high expression of GSDME gene and B lymphocyte infiltration,whereas a significant positive correlation between high GSDME gene and mast cell infiltration in cervical cancer tissues(P＜0.05).The high expression of GSDME in cervical cancer exhibited a significant negative correlation with glutathione peroxidase activity,mRNA modification,keratin filament,and other biological functions(P＜0.001).RT-qPCR and Western blot con-firmed that the transcription and protein expression of GSDME could be detected in both human papilloma virus(HPV)positive and nega-tive cervical cancer cells.Conclusion GSDME may be a risk factor related to cervical cancer,and the correlation analysis between GS-DME and cervical cancer provides a new perspective for the follow-up targeted treatment of cervical cancer based on GSDME.

Objective To investigate the effect of tripterygium glycosides on the resistance to immune rejection after allogeneic islet transplantation in mice.Methods Twenty C57BL/6 mice were treated with STZ diabetes mellitus and transplanted the islets from Balb/c mice donor,then recipient mice were randomly divided into two groups:triptolide group and model control group(n=10),and were intraperitoneal injected with tripterygium glycoside solutin and equivalent solvent of 5 mg·kg-1·d-1 for 14 days.Blood glucose and body weight were measured within 4 weeks after transplantation.Two weeks later,two groups of grafted islets were stained by HE staining and immunohistochemical staining,the expression of IL-2 protein were detected by Western blotting.Results The level of blood glucose were decreased to normal in the triptolide group and model control group after islet transplantation,but blood glucose gradually increased in the model control group after two weeks.Compared with the model control group,the inflammatory cells were less infiltrated and the immunohistochemical staining of insulin was deeper in the triptolide group.The expression of IL-2 in the triptolide group was significantly decreased(P<0.05).Conclusion Tripterygium glucoside could significantly decrease the inflammatory cell infiltration and inflammation factor expression in the allogeneic islet recipients to reduce the immune rejection and improve graft survival.

Objective: To investigate the status of passive smoking among pregnant women in Jinshan District, Shanghai Municipality, so as to provide insights into developing targeted smoking control measures and promoting maternal and infant health. Methods: Pregnant women who had early pregnancy registration at Jinshan District Community Health Service Center from April 2021 to December 2023 were selected as subjects. The basic information, passive smoking and awareness of passive smoking hazards among pregnant women were collected through questionnaire surveys, and passive smoking rate and awareness rate of passive smoking hazards were analyzed. Results: Totally 8 273 questionnaires were allocated, and 8 216 valid questionnaires were recovered, with an effective rate of 99.31%. The mean age of participants was (29.52±4.60) years. There were 4 991 participants with an education of college degree or above, accounting for 60.75%; 3 565 participants with the first pregnancy, accounting for 43.39%; 3 990 primiparas, accounting for 48.56%; 3 193 participants living with smokers, accounting for 38.86%. A total of 3 710 participants passively smoked, with a passive smoking rate of 45.16%. There were 2 817 participants passively smoked in public places, accounting for 75.93%; 2 253 participants passively smoked in workplaces, accounting for 60.73%; 1 563 participants that passively smoked at home, accounting for 42.13%. The awareness rates regarding the hazards of passive smoking to health, causing lung cancer in adults, causing lung diseases in children, causing preterm birth and low birth weight infants, and causing heart diseases in adults were 92.13%, 88.85%, 87.99%, 82.05% and 62.56%, respectively. Conclusion The rate of passive smoking among pregnant women in Jinshan District is comparatively high, while their awareness regarding non-respiratory diseases emanating from passive smoking is comparatively low.

To evaluate the value of left ventricular diastolic function in type 2 diabetes mellitus (DM) using dual-gate Doppler and relative factors, we included 50 non-obesity and hypertension-free DM patients into the controlled group in the study along with 48 age-and-gender-matched healthy volunteer subjects. The peak early diastolic velocity (E), peak later diastolic velocity (A), deceleration time (DT), anterior mitral annulus diastolic peak velocity (e') , isovolumic relaxation time (IVRT), E/A, E/e', Tei index and T(E-e), were measured with dual-gate Doppler. 20 subjects were randomly selected for repetitive analysis. Study showed statistical difference in E/A, DT, e', E/e', IVRT, Tei index and T(E-e), between the two groups (P < 0.05). Linear regression analysis showed positive correlation between T(E-e), and IVRT, course of DM patients and T(E-e), (Beta = 0.295, P = 0.020), and HbA1c control level and T(E-e), (Beta = 0.399, P = 0.010). Repeated analysis showed good reproducibility for both within and between groups. Dual-gate Doppler has clinical value in evaluating left ventricular diastolic function in type 2 diabetes mellitus patients. The course of type 2 diabetes mellitus patients and HbA(1C) control level were both closely related with left ventricular diastolic function.
Case-Control Studies ; Diabetes Mellitus, Type 2 ; physiopathology ; Diastole ; Echocardiography, Doppler ; Humans ; Hypertension ; Mitral Valve ; Regression Analysis ; Reproducibility of Results ; Ventricular Dysfunction, Left ; Ventricular Function, Left

OBJECTIVE:To investigate in vitro antibacterial effect of extracts from Miao medicine Rubus multibracteatus leaves. METHODS:The aqueous extract and 80% ethanol extracts from R. multibracteatus leaves were used to prepare solution with mass concentration of 200 mg/mL. Using ampicillin and fluconazol as positive control (50 mg/mL),cup plate method was used to determine antibacterial effects of the extracts from R. multibracteatus leaves to Staphylococcus aureus,Staphylococcus epi-dermidis,Escherichia coli,Shigella dysenteriae,Proteus vulgaris,Candida albicans and Cryptococcus neoformans. The petroleum ether,acetic ether and n-butyl alcohol were used to extract 80% ethanol extracts in turns. After obtaining relevant extracts (50 mg/mL),cup plate method was used to investigate antibacterial effects of them to above 7 bacterial strains. The parts with antibacte-rial effects and bacterial strains sensitive to drug were screened. Micro-broth dilution method and agar culture medium plate method were used to determine minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC). RESULTS:The aqueous extract of R. multibracteatus leaves almost had no inhibitory effect while 80% ethanol extract showed different degrees of antibacterial effect to tested bacterial strains,and it also had higher activity against 5 bacterial stains than 2 fungus. The ethyl ace-tate and n-butyl alcohol fractions of 80% ethanol extracts showed good effect while the petroleum ether and water layer fractions had no antibacterial effect,and all the fractions of 80% ethanol extracts showed no inhibitory effect on fungus. To 5 bacterial stains,MIC and MBC of 80% ethanol extract were 6.25-12.5,12.5-25 mg/mL,those of ethyl acetate fractions were 3.13,6.25 mg/mL and those of n-butyl alcohol fraction were 3.13-6.25,6.25-12.5 mg/mL,respectively. CONCLUSIONS:80% ethanol ex-tract of Miao medicine R. multibracteatus leaves and its ethyl acetate and n-butyl alcohol fractions have obvious in vitro antibacteri-al effect to bacteria.

Theδ13 C values of volatile organic compounds ( VOCs) in various emission sources and ambient air were analyzed by using thermal desorption coupled with gas chromatography and isotope ratio mass. The lowest sample concentration and peak shape quality needed for high precision and accurate analysis were investigated. Fuel evaporation ( gasoline and diesel) , vehicle exhaust, solvent evaporation, dining fumes and ambient air of different functional zones of Xiamen city were collected using Tenax TA tube, and the significant differences in δ13 C values of VOCs between these sources were observed. The δ13 C value of gasoline exhaust ( 97 # ) was heavier (-25 . 84‰) than that of dining fumes (-30 . 26‰) and theδ13 C values of fuel evaporation were heavier than that of vehicle exhaust after combustion. The average δ13 C value of atmospheric VOCs in Xiamen was at the level of -27 . 03‰ to -25 . 40‰, which was close to the δ13 C value of the evaporation and exhaust of gasoline and diesel, indicating that the VOCs in the atmosphere of Xiamen was highly influenced by transportation related sources.